A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Identifieur interne : 000047 ( PubMed/Corpus ); précédent : 000046; suivant : 000048A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Auteurs : L A Stepanova ; M V Sergeeva ; M A Shuklina ; A A Shaldzhyan ; M V Potapchuk ; A V Korotkov ; L M TsybalovaSource :
- Acta naturae [ 2075-8251 ]
Abstract
Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses.
PubMed: 27437146
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.</title>
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<front><div type="abstract" xml:lang="en">Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses. </div>
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<Abstract><AbstractText>Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses. </AbstractText>
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<ReferenceList><Reference><Citation>Annu Rev Biochem. 2000;69:531-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10966468</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2001 Jan 1;166(1):226-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11123296</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2002 Oct 1;169(7):3914-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12244190</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 2004 May;72(5):2810-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15102791</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2004 Jun 1;172(11):6922-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15153511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comput Chem. 2004 Oct;25(13):1605-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15264254</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Immunol. 2004 Nov;34(11):2986-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15384042</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Scand J Immunol. 2005 Jun;61(6):503-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15963044</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2006 Apr;12(4):569-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16704803</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2007 Jan 8;25(4):763-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16968658</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 1991 Dec 15;147(12):4054-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1721638</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2007 Mar;88(Pt 3):951-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17325369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008;3(12):e3942</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19079604</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 Mar;83(6):2553-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19109379</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Immunol. 2009 Feb;39(2):359-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19152336</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2009;4(3):363-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19247286</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Immunol. 2009 Jun;10(6):573-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19448659</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2009 Jun 15;182(12):7539-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19494277</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2010 Jan;38(Database issue):D854-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19906713</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2010 Feb 26;6(2):e1000796</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20195520</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunogenetics. 2010 Jun;62(6):357-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20379710</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2010 Jun 11;328(5984):1394-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20538950</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13701-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20615991</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2010 Aug 27;329(5995):1060-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20647428</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18979-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20956293</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 2011 Jan 17;208(1):181-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21220454</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Immunol. 2011 Jun;41(6):1606-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21469112</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2011 Jun 1;186(11):6255-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21515787</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1990 Jun;87(12):4645-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2162051</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2011 Aug 12;333(6044):843-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21737702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2011 Aug 12;333(6044):850-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21798894</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2013 Jan;97(1):24-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23036818</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Immunol. 1995 Dec;7(6):347-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8775460</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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