A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Identifieur interne : 000047 ( PubMed/Corpus ); précédent : 000046; suivant : 000048A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Auteurs : L A Stepanova ; M V Sergeeva ; M A Shuklina ; A A Shaldzhyan ; M V Potapchuk ; A V Korotkov ; L M TsybalovaSource :
- Acta naturae [ 2075-8251 ]
Abstract
Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses.
PubMed: 27437146
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.</title><author><name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Sergeeva, M V" sort="Sergeeva, M V" uniqKey="Sergeeva M" first="M V" last="Sergeeva">M V Sergeeva</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Shuklina, M A" sort="Shuklina, M A" uniqKey="Shuklina M" first="M A" last="Shuklina">M A Shuklina</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Shaldzhyan, A A" sort="Shaldzhyan, A A" uniqKey="Shaldzhyan A" first="A A" last="Shaldzhyan">A A Shaldzhyan</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2016 Apr-Jun</MedlineDate></PubDate></date><idno type="RBID">pubmed:27437146</idno><idno type="pmid">27437146</idno><idno type="wicri:Area/PubMed/Corpus">000047</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000047</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.</title><author><name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Sergeeva, M V" sort="Sergeeva, M V" uniqKey="Sergeeva M" first="M V" last="Sergeeva">M V Sergeeva</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Shuklina, M A" sort="Shuklina, M A" uniqKey="Shuklina M" first="M A" last="Shuklina">M A Shuklina</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Shaldzhyan, A A" sort="Shaldzhyan, A A" uniqKey="Shaldzhyan A" first="A A" last="Shaldzhyan">A A Shaldzhyan</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name><affiliation><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Acta naturae</title><idno type="ISSN">2075-8251</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27437146</PMID><DateCompleted><Year>2016</Year><Month>07</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>11</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">2075-8251</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>2</Issue><PubDate><MedlineDate>2016 Apr-Jun</MedlineDate></PubDate></JournalIssue><Title>Acta naturae</Title><ISOAbbreviation>Acta Naturae</ISOAbbreviation></Journal><ArticleTitle>A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.</ArticleTitle><Pagination><MedlinePgn>116-26</MedlinePgn></Pagination><Abstract><AbstractText>Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stepanova</LastName><ForeName>L A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sergeeva</LastName><ForeName>M V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shuklina</LastName><ForeName>M A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shaldzhyan</LastName><ForeName>A A</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Potapchuk</LastName><ForeName>M V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Korotkov</LastName><ForeName>A V</ForeName><Initials>AV</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsybalova</LastName><ForeName>L M</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Russia (Federation)</Country><MedlineTA>Acta Naturae</MedlineTA><NlmUniqueID>101525823</NlmUniqueID><ISSNLinking>2075-8251</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">HA2</Keyword><Keyword MajorTopicYN="N">flagellin</Keyword><Keyword MajorTopicYN="N">influenza</Keyword><Keyword MajorTopicYN="N">recombinant protein</Keyword><Keyword MajorTopicYN="N">vaccine</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>7</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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