Platform technology to generate broadly cross-reactive antibodies to α-helical epitopes in hemagglutinin proteins from influenza A viruses.
Identifieur interne : 000C57 ( Ncbi/Checkpoint ); précédent : 000C56; suivant : 000C58Platform technology to generate broadly cross-reactive antibodies to α-helical epitopes in hemagglutinin proteins from influenza A viruses.
Auteurs : Ziqing Jiang ; Lajos Gera ; Colin T. Mant ; Brooke Hirsch ; Zhe Yan ; Jonathan A. Shortt ; David D. Pollock ; Zhaohui Qian [République populaire de Chine] ; Kathryn V. Holmes ; Robert S. HodgesSource :
- Biopolymers [ 1097-0282 ] ; 2016.
Abstract
We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on the surface of the two-stranded α-helical coiled-coil template. This template when used as an immunogen elicits polyclonal antibodies which bind to the α-helix in the native protein. We investigated the highly conserved sequence region 421-476 of HA by inserting 21 or 28 residue sequences from this region into our template. The cross-reactivity of the resulting rabbit polyclonal antibodies prepared to these immunogens was determined using a series of HA proteins from H1N1, H2N2, H3N2, H5N1, H7N7, and H7N9 virus strains which are representative of Group 1 and Group 2 virus subtypes of influenza A. Antibodies from region 449-476 were Group 1 specific. Antibodies to region 421-448 showed the greatest degree of cross-reactivity to Group 1 and Group 2 and suggested that this region has a great potential as a "universal" synthetic peptide vaccine for influenza A. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 144-159, 2016.
DOI: 10.1002/bip.22808
PubMed: 26799790
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000053
- to stream PubMed, to step Curation: 000053
- to stream PubMed, to step Checkpoint: 000045
- to stream Ncbi, to step Merge: 000C57
- to stream Ncbi, to step Curation: 000C57
Links to Exploration step
pubmed:26799790Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Platform technology to generate broadly cross-reactive antibodies to α-helical epitopes in hemagglutinin proteins from influenza A viruses.</title><author><name sortKey="Jiang, Ziqing" sort="Jiang, Ziqing" uniqKey="Jiang Z" first="Ziqing" last="Jiang">Ziqing Jiang</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Gera, Lajos" sort="Gera, Lajos" uniqKey="Gera L" first="Lajos" last="Gera">Lajos Gera</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Mant, Colin T" sort="Mant, Colin T" uniqKey="Mant C" first="Colin T" last="Mant">Colin T. Mant</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Hirsch, Brooke" sort="Hirsch, Brooke" uniqKey="Hirsch B" first="Brooke" last="Hirsch">Brooke Hirsch</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Yan, Zhe" sort="Yan, Zhe" uniqKey="Yan Z" first="Zhe" last="Yan">Zhe Yan</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Shortt, Jonathan A" sort="Shortt, Jonathan A" uniqKey="Shortt J" first="Jonathan A" last="Shortt">Jonathan A. Shortt</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Pollock, David D" sort="Pollock, David D" uniqKey="Pollock D" first="David D" last="Pollock">David D. Pollock</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Qian, Zhaohui" sort="Qian, Zhaohui" uniqKey="Qian Z" first="Zhaohui" last="Qian">Zhaohui Qian</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Pathogen Biology at Chinese Academy of Medical Sciences, Yi Zhuang DiShengBeiLu, BeiGongDa RuanJianYuan, Bldg#7, Beijing, 100176, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Pathogen Biology at Chinese Academy of Medical Sciences, Yi Zhuang DiShengBeiLu, BeiGongDa RuanJianYuan, Bldg#7, Beijing, 100176</wicri:regionArea><wicri:noRegion>100176</wicri:noRegion></affiliation></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name><affiliation><nlm:affiliation>Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Hodges, Robert S" sort="Hodges, Robert S" uniqKey="Hodges R" first="Robert S" last="Hodges">Robert S. Hodges</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26799790</idno><idno type="pmid">26799790</idno><idno type="doi">10.1002/bip.22808</idno><idno type="wicri:Area/PubMed/Corpus">000053</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000053</idno><idno type="wicri:Area/PubMed/Curation">000053</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000053</idno><idno type="wicri:Area/PubMed/Checkpoint">000045</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000045</idno><idno type="wicri:Area/Ncbi/Merge">000C57</idno><idno type="wicri:Area/Ncbi/Curation">000C57</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C57</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Platform technology to generate broadly cross-reactive antibodies to α-helical epitopes in hemagglutinin proteins from influenza A viruses.</title><author><name sortKey="Jiang, Ziqing" sort="Jiang, Ziqing" uniqKey="Jiang Z" first="Ziqing" last="Jiang">Ziqing Jiang</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Gera, Lajos" sort="Gera, Lajos" uniqKey="Gera L" first="Lajos" last="Gera">Lajos Gera</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Mant, Colin T" sort="Mant, Colin T" uniqKey="Mant C" first="Colin T" last="Mant">Colin T. Mant</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Hirsch, Brooke" sort="Hirsch, Brooke" uniqKey="Hirsch B" first="Brooke" last="Hirsch">Brooke Hirsch</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Yan, Zhe" sort="Yan, Zhe" uniqKey="Yan Z" first="Zhe" last="Yan">Zhe Yan</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Shortt, Jonathan A" sort="Shortt, Jonathan A" uniqKey="Shortt J" first="Jonathan A" last="Shortt">Jonathan A. Shortt</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Pollock, David D" sort="Pollock, David D" uniqKey="Pollock D" first="David D" last="Pollock">David D. Pollock</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Qian, Zhaohui" sort="Qian, Zhaohui" uniqKey="Qian Z" first="Zhaohui" last="Qian">Zhaohui Qian</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Pathogen Biology at Chinese Academy of Medical Sciences, Yi Zhuang DiShengBeiLu, BeiGongDa RuanJianYuan, Bldg#7, Beijing, 100176, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Pathogen Biology at Chinese Academy of Medical Sciences, Yi Zhuang DiShengBeiLu, BeiGongDa RuanJianYuan, Bldg#7, Beijing, 100176</wicri:regionArea><wicri:noRegion>100176</wicri:noRegion></affiliation></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name><affiliation><nlm:affiliation>Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author><author><name sortKey="Hodges, Robert S" sort="Hodges, Robert S" uniqKey="Hodges R" first="Robert S" last="Hodges">Robert S. Hodges</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Genetics, University of Colorado, Anschutz Medical Campus, School of Medicine, Aurora, CO, 80045.</nlm:affiliation><wicri:noCountry code="subField">80045</wicri:noCountry></affiliation></author></analytic><series><title level="j">Biopolymers</title><idno type="eISSN">1097-0282</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on the surface of the two-stranded α-helical coiled-coil template. This template when used as an immunogen elicits polyclonal antibodies which bind to the α-helix in the native protein. We investigated the highly conserved sequence region 421-476 of HA by inserting 21 or 28 residue sequences from this region into our template. The cross-reactivity of the resulting rabbit polyclonal antibodies prepared to these immunogens was determined using a series of HA proteins from H1N1, H2N2, H3N2, H5N1, H7N7, and H7N9 virus strains which are representative of Group 1 and Group 2 virus subtypes of influenza A. Antibodies from region 449-476 were Group 1 specific. Antibodies to region 421-448 showed the greatest degree of cross-reactivity to Group 1 and Group 2 and suggested that this region has a great potential as a "universal" synthetic peptide vaccine for influenza A. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 144-159, 2016.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Gera, Lajos" sort="Gera, Lajos" uniqKey="Gera L" first="Lajos" last="Gera">Lajos Gera</name><name sortKey="Hirsch, Brooke" sort="Hirsch, Brooke" uniqKey="Hirsch B" first="Brooke" last="Hirsch">Brooke Hirsch</name><name sortKey="Hodges, Robert S" sort="Hodges, Robert S" uniqKey="Hodges R" first="Robert S" last="Hodges">Robert S. Hodges</name><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name><name sortKey="Jiang, Ziqing" sort="Jiang, Ziqing" uniqKey="Jiang Z" first="Ziqing" last="Jiang">Ziqing Jiang</name><name sortKey="Mant, Colin T" sort="Mant, Colin T" uniqKey="Mant C" first="Colin T" last="Mant">Colin T. Mant</name><name sortKey="Pollock, David D" sort="Pollock, David D" uniqKey="Pollock D" first="David D" last="Pollock">David D. Pollock</name><name sortKey="Shortt, Jonathan A" sort="Shortt, Jonathan A" uniqKey="Shortt J" first="Jonathan A" last="Shortt">Jonathan A. Shortt</name><name sortKey="Yan, Zhe" sort="Yan, Zhe" uniqKey="Yan Z" first="Zhe" last="Yan">Zhe Yan</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Qian, Zhaohui" sort="Qian, Zhaohui" uniqKey="Qian Z" first="Zhaohui" last="Qian">Zhaohui Qian</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C57 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000C57 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:26799790
|texte= Platform technology to generate broadly cross-reactive antibodies to α-helical epitopes in hemagglutinin proteins from influenza A viruses.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:26799790" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |