The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus
Identifieur interne : 000427 ( Ncbi/Checkpoint ); précédent : 000426; suivant : 000428The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus
Auteurs : Amorsolo L. Suguitan ; Michael P. Marino ; Purvi D. Desai ; Li-Mei Chen ; Yumiko Matsuoka ; Ruben O. Donis ; Hong Jin ; David E. Swayne ; George Kemble ; Kanta SubbaraoSource :
- Virology [ 0042-6822 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN viral, Adaptation physiologique, Animaux, Basse température, Calendrier d'administration des médicaments, Femelle, Glycoprotéine hémagglutinine du virus influenza (), Infections à Orthomyxoviridae (), Poulets, Protéines recombinantes, Protéines virales, Souris, Souris de lignée BALB C, Sous-type H5N1 du virus de la grippe A (physiologie), Séquence d'acides aminés, Séquence nucléotidique, Vaccins antigrippaux (immunologie), Vaccins atténués (immunologie).
- MESH :
- immunologie : Vaccins antigrippaux, Vaccins atténués.
- physiologie : Sous-type H5N1 du virus de la grippe A.
- ADN viral, Adaptation physiologique, Animaux, Basse température, Calendrier d'administration des médicaments, Femelle, Glycoprotéine hémagglutinine du virus influenza, Infections à Orthomyxoviridae, Poulets, Protéines recombinantes, Protéines virales, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Adaptation, Physiological, Amino Acid Sequence, Animals, Base Sequence, Chickens, Cold Temperature, DNA, Viral, Drug Administration Schedule, Female, Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Influenza A Virus, H5N1 Subtype (physiology), Influenza Vaccines (immunology), Mice, Mice, Inbred BALB C, Orthomyxoviridae Infections (prevention & control), Recombinant Proteins, Vaccines, Attenuated (immunology), Viral Proteins.
- MESH :
- chemical , chemistry : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , immunology : Influenza Vaccines, Vaccines, Attenuated.
- chemical : DNA, Viral, Recombinant Proteins, Viral Proteins.
- physiology : Influenza A Virus, H5N1 Subtype.
- prevention & control : Orthomyxoviridae Infections.
- Adaptation, Physiological, Amino Acid Sequence, Animals, Base Sequence, Chickens, Cold Temperature, Drug Administration Schedule, Female, Mice, Mice, Inbred BALB C.
Abstract
A recombinant live attenuated influenza virus ΔH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA
Url:
DOI: 10.1016/j.virol.2009.09.017
PubMed: 19833372
PubMed Central: 2787913
Affiliations:
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PMC:2787913Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The influence of the multi-basic cleavage site of the H5 hemagglutinin on the attenuation, immunogenicity and efficacy of a live attenuated influenza A H5N1 cold-adapted vaccine virus</title>
<author><name sortKey="Suguitan, Amorsolo L" sort="Suguitan, Amorsolo L" uniqKey="Suguitan A" first="Amorsolo L." last="Suguitan">Amorsolo L. Suguitan</name>
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<wicri:noCountry code="subfield">USA 20892-3203</wicri:noCountry>
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<author><name sortKey="Marino, Michael P" sort="Marino, Michael P" uniqKey="Marino M" first="Michael P." last="Marino">Michael P. Marino</name>
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<wicri:noCountry code="subfield">USA 20892-3203</wicri:noCountry>
</affiliation>
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<author><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
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<wicri:noCountry code="subfield">USA 30333</wicri:noCountry>
</affiliation>
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<author><name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name>
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</affiliation>
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<author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O." last="Donis">Ruben O. Donis</name>
<affiliation><nlm:aff id="A2">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia, USA 30333</nlm:aff>
<wicri:noCountry code="subfield">USA 30333</wicri:noCountry>
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<author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name>
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<wicri:noCountry code="subfield">USA 94043</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E." last="Swayne">David E. Swayne</name>
<affiliation><nlm:aff id="A4">Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, 934 College Station Road, Athens, Georgia, USA 30605</nlm:aff>
<wicri:noCountry code="subfield">USA 30605</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name>
<affiliation><nlm:aff id="A3">MedImmune, 297 N Bernardo Avenue, Mountain View, California, USA 94043</nlm:aff>
<wicri:noCountry code="subfield">USA 94043</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
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<series><title level="j">Virology</title>
<idno type="ISSN">0042-6822</idno>
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<imprint><date when="2009">2009</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological</term>
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Chickens</term>
<term>Cold Temperature</term>
<term>DNA, Viral</term>
<term>Drug Administration Schedule</term>
<term>Female</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (chemistry)</term>
<term>Influenza A Virus, H5N1 Subtype (physiology)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Orthomyxoviridae Infections (prevention & control)</term>
<term>Recombinant Proteins</term>
<term>Vaccines, Attenuated (immunology)</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral</term>
<term>Adaptation physiologique</term>
<term>Animaux</term>
<term>Basse température</term>
<term>Calendrier d'administration des médicaments</term>
<term>Femelle</term>
<term>Glycoprotéine hémagglutinine du virus influenza ()</term>
<term>Infections à Orthomyxoviridae ()</term>
<term>Poulets</term>
<term>Protéines recombinantes</term>
<term>Protéines virales</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Sous-type H5N1 du virus de la grippe A (physiologie)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins atténués (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Influenza Vaccines</term>
<term>Vaccines, Attenuated</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Viral</term>
<term>Recombinant Proteins</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Vaccins antigrippaux</term>
<term>Vaccins atténués</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term>
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Chickens</term>
<term>Cold Temperature</term>
<term>Drug Administration Schedule</term>
<term>Female</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term>
<term>Adaptation physiologique</term>
<term>Animaux</term>
<term>Basse température</term>
<term>Calendrier d'administration des médicaments</term>
<term>Femelle</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Infections à Orthomyxoviridae</term>
<term>Poulets</term>
<term>Protéines recombinantes</term>
<term>Protéines virales</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en"><p id="P4">A recombinant live attenuated influenza virus ΔH5N1 vaccine with a modified hemagglutinin (HA) and intact neuraminidase genes from A/Vietnam/1203/04 (H5N1) and six remaining genome segments from A/Ann Arbor/6/60 (H2N2) cold-adapted (AA <italic>ca</italic>
) virus was previously shown to be attenuated in chickens, mice and ferrets. Evaluation of the recombinant H5N1 viruses in mice indicated that three independent factors contributed to the attenuation of the ΔH5N1 vaccine: the attenuating mutations specified by the AA <italic>ca</italic>
loci had the greatest influence, followed by the deletion of the H5 HA multi-basic cleavage site (MBS), and the constellation effects of the AA genes acting in concert with the H5N1 glycoproteins. Restoring the MBS in the H5 HA of the vaccine virus improved its immunogenicity and efficacy, likely as a consequence of increased virus replication, indicating that removal of the MBS had a deleterious effect on the immunogenicity and efficacy of the ΔH5N1 vaccine in mice.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name>
<name sortKey="Desai, Purvi D" sort="Desai, Purvi D" uniqKey="Desai P" first="Purvi D." last="Desai">Purvi D. Desai</name>
<name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O." last="Donis">Ruben O. Donis</name>
<name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name>
<name sortKey="Kemble, George" sort="Kemble, George" uniqKey="Kemble G" first="George" last="Kemble">George Kemble</name>
<name sortKey="Marino, Michael P" sort="Marino, Michael P" uniqKey="Marino M" first="Michael P." last="Marino">Michael P. Marino</name>
<name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name>
<name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<name sortKey="Suguitan, Amorsolo L" sort="Suguitan, Amorsolo L" uniqKey="Suguitan A" first="Amorsolo L." last="Suguitan">Amorsolo L. Suguitan</name>
<name sortKey="Swayne, David E" sort="Swayne, David E" uniqKey="Swayne D" first="David E." last="Swayne">David E. Swayne</name>
</noCountry>
</tree>
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</record>
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