Studies with a Cold-Recombinant A/Victoria/3/7S (H3N2) Virus. I. Biologic, Genetic, and Biochemical Characterization
Identifieur interne : 002944 ( Main/Merge ); précédent : 002943; suivant : 002945Studies with a Cold-Recombinant A/Victoria/3/7S (H3N2) Virus. I. Biologic, Genetic, and Biochemical Characterization
Auteurs : P. Reeve [Autriche, États-Unis, Royaume-Uni] ; J. W. Almond [Autriche, États-Unis] ; V. Felsenreich [Autriche, États-Unis] ; M. Pibermann [Autriche, États-Unis] ; H. F. Maassab [Autriche, États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1980.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes viraux.
- génétique : ARN viral, Virus de la grippe A.
- immunologie : Vaccins antigrippaux, Virus de la grippe A.
- Animaux, Basse température, Embryon de poulet, Mutation, Recombinaison génétique, Sous-type H3N2 du virus de la grippe A, Variation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- genetics : Influenza A virus, RNA, Viral.
- immunology : Influenza A virus, Influenza Vaccines.
- Animals, Chick Embryo, Cold Temperature, Genetic Variation, Influenza A Virus, H3N2 Subtype, Mutation, Recombination, Genetic.
Abstract
A cold-recombinant virus, CR 22, was derived from an attenuated cold-adapted parent strain, AIAnn Arbor/6/60 (H2N2), and a wild-type parent strain, A/Victoria/3/75 (H3N2). Antigenic analysis showed that CR 22 possesses the hemagglutinin and neuraminidase surface antigens derived from the A/Victoria/3/75 (H3N2) parent. From studies of virus-induced polypeptides using polyacrylamide gel electrophoresis, it was deduced that a polymerase protein, PI, is coded for by an RNA segment derived from the wild-type parent; all other genetic elements are derived from the attenuated parent. The attenuated parent and the recombinant CR 22 both possess temperature-sensitive (ts) lesions, evident by restriction of multiplication in fertile chicken eggs or in MadinDarby canine kidney cell cultures at ⩾38 C. Genetic analysis of CR 22 by complementation tests using Hong Kong and Wilson Smith neurotropic ts mutants gave evidence for a ts lesion in the genetic elements coding for complementary RNA.
Url:
DOI: 10.1093/infdis/142.6.850
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Antigenic analysis showed that CR 22 possesses the hemagglutinin and neuraminidase surface antigens derived from the A/Victoria/3/75 (H3N2) parent. From studies of virus-induced polypeptides using polyacrylamide gel electrophoresis, it was deduced that a polymerase protein, PI, is coded for by an RNA segment derived from the wild-type parent; all other genetic elements are derived from the attenuated parent. The attenuated parent and the recombinant CR 22 both possess temperature-sensitive (ts) lesions, evident by restriction of multiplication in fertile chicken eggs or in MadinDarby canine kidney cell cultures at ⩾38 C. Genetic analysis of CR 22 by complementation tests using Hong Kong and Wilson Smith neurotropic ts mutants gave evidence for a ts lesion in the genetic elements coding for complementary RNA.</div></front></TEI><double doi="10.1093/infdis/142.6.850"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Studies with a Cold-Recombinant A/Victoria/3/7S (H3N2) Virus. I. Biologic, Genetic, and Biochemical Characterization</title><author><name sortKey="Reeve, P" sort="Reeve, P" uniqKey="Reeve P" first="P." last="Reeve">P. Reeve</name></author><author><name sortKey="Almond, J W" sort="Almond, J W" uniqKey="Almond J" first="J. W." last="Almond">J. W. Almond</name></author><author><name sortKey="Felsenreich, V" sort="Felsenreich, V" uniqKey="Felsenreich V" first="V." last="Felsenreich">V. Felsenreich</name></author><author><name sortKey="Pibermann, M" sort="Pibermann, M" uniqKey="Pibermann M" first="M." last="Pibermann">M. Pibermann</name></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H. F." last="Maassab">H. F. Maassab</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:64779958AC9952D6D42ABEBC4A9078777756FCA4</idno><date when="1980" year="1980">1980</date><idno type="doi">10.1093/infdis/142.6.850</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-HRL1HQ19-7/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000260</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000260</idno><idno type="wicri:Area/Istex/Curation">000260</idno><idno type="wicri:Area/Istex/Checkpoint">001411</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001411</idno><idno type="wicri:doubleKey">0022-1899:1980:Reeve P:studies:with:a</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Studies with a Cold-Recombinant A/Victoria/3/7S (H3N2) Virus. I. Biologic, Genetic, and Biochemical Characterization</title><author><name sortKey="Reeve, P" sort="Reeve, P" uniqKey="Reeve P" first="P." last="Reeve">P. Reeve</name><affiliation wicri:level="3"><country xml:lang="fr">Autriche</country><wicri:regionArea>Sandoz Forschungsinstitut, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>School of Public Health, University of Michigan, Ann Arbor</wicri:cityArea></affiliation><affiliation wicri:level="2"><country>Royaume-Uni</country><placeName><region type="country">Angleterre</region></placeName><wicri:cityArea>Please address requests for reprints to Dr. P. Reeve at his present address: National Institute for Biological Standards and Control, London NW3</wicri:cityArea></affiliation></author><author><name sortKey="Almond, J W" sort="Almond, J W" uniqKey="Almond J" first="J. W." last="Almond">J. W. Almond</name><affiliation wicri:level="3"><country xml:lang="fr">Autriche</country><wicri:regionArea>Sandoz Forschungsinstitut, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>School of Public Health, University of Michigan, Ann Arbor</wicri:cityArea></affiliation></author><author><name sortKey="Felsenreich, V" sort="Felsenreich, V" uniqKey="Felsenreich V" first="V." last="Felsenreich">V. Felsenreich</name><affiliation wicri:level="3"><country xml:lang="fr">Autriche</country><wicri:regionArea>Sandoz Forschungsinstitut, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>School of Public Health, University of Michigan, Ann Arbor</wicri:cityArea></affiliation></author><author><name sortKey="Pibermann, M" sort="Pibermann, M" uniqKey="Pibermann M" first="M." last="Pibermann">M. Pibermann</name><affiliation wicri:level="3"><country xml:lang="fr">Autriche</country><wicri:regionArea>Sandoz Forschungsinstitut, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>School of Public Health, University of Michigan, Ann Arbor</wicri:cityArea></affiliation></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H. F." last="Maassab">H. F. Maassab</name><affiliation wicri:level="3"><country xml:lang="fr">Autriche</country><wicri:regionArea>Sandoz Forschungsinstitut, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>School of Public Health, University of Michigan, Ann Arbor</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Infectious Diseases</title><title level="j" type="abbrev">J Infect Dis.</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date when="1980-12">1980</date><biblScope unit="vol">142</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="850">850</biblScope><biblScope unit="page" to="856">856</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">A cold-recombinant virus, CR 22, was derived from an attenuated cold-adapted parent strain, AIAnn Arbor/6/60 (H2N2), and a wild-type parent strain, A/Victoria/3/75 (H3N2). Antigenic analysis showed that CR 22 possesses the hemagglutinin and neuraminidase surface antigens derived from the A/Victoria/3/75 (H3N2) parent. From studies of virus-induced polypeptides using polyacrylamide gel electrophoresis, it was deduced that a polymerase protein, PI, is coded for by an RNA segment derived from the wild-type parent; all other genetic elements are derived from the attenuated parent. The attenuated parent and the recombinant CR 22 both possess temperature-sensitive (ts) lesions, evident by restriction of multiplication in fertile chicken eggs or in MadinDarby canine kidney cell cultures at ⩾38 C. Genetic analysis of CR 22 by complementation tests using Hong Kong and Wilson Smith neurotropic ts mutants gave evidence for a ts lesion in the genetic elements coding for complementary RNA.</div></front></TEI></ISTEX><PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Studies with a cold-recombinant A/Victoria/3/75 (H3N2) virus. I. biologic, genetic, and biochemical characterization.</title><author><name sortKey="Reeve, P" sort="Reeve, P" uniqKey="Reeve P" first="P" last="Reeve">P. Reeve</name></author><author><name sortKey="Almond, J W" sort="Almond, J W" uniqKey="Almond J" first="J W" last="Almond">J W Almond</name></author><author><name sortKey="Felsenreich, V" sort="Felsenreich, V" uniqKey="Felsenreich V" first="V" last="Felsenreich">V. Felsenreich</name></author><author><name sortKey="Pibermann, M" sort="Pibermann, M" uniqKey="Pibermann M" first="M" last="Pibermann">M. Pibermann</name></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1980">1980</date><idno type="RBID">pubmed:7462696</idno><idno type="pmid">7462696</idno><idno type="doi">10.1093/infdis/142.6.850</idno><idno type="wicri:Area/PubMed/Corpus">000586</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000586</idno><idno type="wicri:Area/PubMed/Curation">000586</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000586</idno><idno type="wicri:Area/PubMed/Checkpoint">000506</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000506</idno><idno type="wicri:Area/Ncbi/Merge">001284</idno><idno type="wicri:Area/Ncbi/Curation">001284</idno><idno type="wicri:Area/Ncbi/Checkpoint">001284</idno><idno type="wicri:doubleKey">0022-1899:1980:Reeve P:studies:with:a</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Studies with a cold-recombinant A/Victoria/3/75 (H3N2) virus. I. biologic, genetic, and biochemical characterization.</title><author><name sortKey="Reeve, P" sort="Reeve, P" uniqKey="Reeve P" first="P" last="Reeve">P. Reeve</name></author><author><name sortKey="Almond, J W" sort="Almond, J W" uniqKey="Almond J" first="J W" last="Almond">J W Almond</name></author><author><name sortKey="Felsenreich, V" sort="Felsenreich, V" uniqKey="Felsenreich V" first="V" last="Felsenreich">V. Felsenreich</name></author><author><name sortKey="Pibermann, M" sort="Pibermann, M" uniqKey="Pibermann M" first="M" last="Pibermann">M. Pibermann</name></author><author><name sortKey="Maassab, H F" sort="Maassab, H F" uniqKey="Maassab H" first="H F" last="Maassab">H F Maassab</name></author></analytic><series><title level="j">The Journal of infectious diseases</title><idno type="ISSN">0022-1899</idno><imprint><date when="1980" type="published">1980</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Viral (analysis)</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Genetic Variation</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Influenza Vaccines (immunology)</term><term>Mutation</term><term>RNA, Viral (genetics)</term><term>Recombination, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Animaux</term><term>Antigènes viraux (analyse)</term><term>Basse température</term><term>Embryon de poulet</term><term>Mutation</term><term>Recombinaison génétique</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Vaccins antigrippaux (immunologie)</term><term>Variation génétique</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Vaccins antigrippaux</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Genetic Variation</term><term>Influenza A Virus, H3N2 Subtype</term><term>Mutation</term><term>Recombination, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Embryon de poulet</term><term>Mutation</term><term>Recombinaison génétique</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cold-recombinant virus CR 22, was derived from an attenuated cold-adapted parent strain. A/Ann Arbor/6/60 (H2N2), and a wild-type parent strain, A/Victoria/3/75 (H3N2). Antigenic analysis showed that CR 22 possesses the hemagglutinin and neruaminidase surface antigens derived from the A/Victoria/3/75 (H3N2) parent. From studies of virus-induced polypeptides using polyacrylamide gel electrophoresis, it was deduced that a polymerase protein, P1, is coded and by an RNA segment derived from the wild-type parent; all other genetic elements are derived from the attenuated parent. The attenuated parent and the recombinant CR 22 both possess temperature-sensitive (ts) lesions, evident by restriction of multiplication in fertile chicken eggs or in Madin-Darby canine kidney cell cultures at greater than or equal to 38 C. Genetic analysis of CR 22 by complementation tests using Hong Kong and Wilson Smith neurotropic ts mutants gave evidence for a ts lesion in the genetic elements coding for complementary RNA.</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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