[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].
Identifieur interne : 001A89 ( Main/Merge ); précédent : 001A88; suivant : 001A90[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].
Auteurs : H. Shimizu ; S. Watanabe ; C. Kawakami ; Y. Hirai ; K. Kimura ; N. Sugaya ; M. ImaiSource :
- Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases [ 0387-5911 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes viraux.
- diagnostic : Grippe humaine.
- immunologie : Virus de la grippe A.
- Enfant, Humains, Sensibilité et spécificité, Test ELISA.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Influenza, Human.
- immunology : Influenza A virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Child, Humans, Sensitivity and Specificity.
Abstract
A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.
DOI: 10.11150/kansenshogakuzasshi1970.72.827
PubMed: 9780586
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pubmed:9780586Le document en format XML
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<term>Humans</term>
<term>Influenza A virus (immunology)</term>
<term>Influenza, Human (diagnosis)</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux (analyse)</term>
<term>Enfant</term>
<term>Grippe humaine (diagnostic)</term>
<term>Humains</term>
<term>Sensibilité et spécificité</term>
<term>Test ELISA ()</term>
<term>Virus de la grippe A (immunologie)</term>
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<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en">A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.</div>
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