[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].
Identifieur interne : 000380 ( PubMed/Curation ); précédent : 000379; suivant : 000381[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].
Auteurs : H. Shimizu ; S. Watanabe ; C. Kawakami ; Y. Hirai ; K. Kimura ; N. Sugaya ; M. ImaiSource :
- Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases [ 0387-5911 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes viraux.
- diagnostic : Grippe humaine.
- immunologie : Virus de la grippe A.
- Enfant, Humains, Sensibilité et spécificité, Test ELISA.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Influenza, Human.
- immunology : Influenza A virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Child, Humans, Sensitivity and Specificity.
Abstract
A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.
DOI: 10.11150/kansenshogakuzasshi1970.72.827
PubMed: 9780586
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Kimura</name></author><author><name sortKey="Sugaya, N" sort="Sugaya, N" uniqKey="Sugaya N" first="N" last="Sugaya">N. Sugaya</name></author><author><name sortKey="Imai, M" sort="Imai, M" uniqKey="Imai M" first="M" last="Imai">M. 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Shimizu</name><affiliation><nlm:affiliation>Kawasaki City Institute of Public Health.</nlm:affiliation><wicri:noCountry code="no comma">Kawasaki City Institute of Public Health.</wicri:noCountry></affiliation></author><author><name sortKey="Watanabe, S" sort="Watanabe, S" uniqKey="Watanabe S" first="S" last="Watanabe">S. Watanabe</name></author><author><name sortKey="Kawakami, C" sort="Kawakami, C" uniqKey="Kawakami C" first="C" last="Kawakami">C. Kawakami</name></author><author><name sortKey="Hirai, Y" sort="Hirai, Y" uniqKey="Hirai Y" first="Y" last="Hirai">Y. Hirai</name></author><author><name sortKey="Kimura, K" sort="Kimura, K" uniqKey="Kimura K" first="K" last="Kimura">K. Kimura</name></author><author><name sortKey="Sugaya, N" sort="Sugaya, N" uniqKey="Sugaya N" first="N" last="Sugaya">N. Sugaya</name></author><author><name sortKey="Imai, M" sort="Imai, M" uniqKey="Imai M" first="M" last="Imai">M. Imai</name></author></analytic><series><title level="j">Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases</title><idno type="ISSN">0387-5911</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (analysis)</term><term>Child</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Influenza A virus (immunology)</term><term>Influenza, Human (diagnosis)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux (analyse)</term><term>Enfant</term><term>Grippe humaine (diagnostic)</term><term>Humains</term><term>Sensibilité et spécificité</term><term>Test ELISA ()</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Child</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Enfant</term><term>Humains</term><term>Sensibilité et spécificité</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9780586</PMID><DateCompleted><Year>1998</Year><Month>12</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0387-5911</ISSN><JournalIssue CitedMedium="Print"><Volume>72</Volume><Issue>8</Issue><PubDate><Year>1998</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases</Title><ISOAbbreviation>Kansenshogaku Zasshi</ISOAbbreviation></Journal><ArticleTitle>[Evaluation of a rapid enzyme immunoassay membrane test for diagnosis of influenza A virus infection].</ArticleTitle><Pagination><MedlinePgn>827-33</MedlinePgn></Pagination><Abstract><AbstractText>A rapid enzyme immunoassay membrane test, Directigen Flu A (Becton Dickinson, USA), was evaluated by using virus isolates and clinical specimens. The reference laboratory diagnosis was based on the results of virus isolation. Directigen Flu A was reactive for all subtypes of human influenza A viruses, including reference strains of H1N1, H2N2 and H3N2. Moreover, H5N1 (Hongkong/156/97) was also detected by this kit. No cross reactivity was detected with other respiratory viruses. Directigen Flu A showed positive reaction with the solution containing influenza A virus of 2.4 x 10(3) pfu/assay. The rapid test demonstrated 77.9% sensitivity and 98.4% specificity for testing of throat swabs from children with respiratory symptoms. It showed higher sensitivity and specificity (92.1% and 100%) for testing of nasopharyngeal aspirates. Directigen Flu A should be useful for the rapid diagnosis of influenza A virus infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shimizu</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Kawasaki City Institute of Public Health.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Watanabe</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kawakami</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Hirai</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Kimura</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Sugaya</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Imai</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>jpn</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Kansenshogaku Zasshi</MedlineTA><NlmUniqueID>0236671</NlmUniqueID><ISSNLinking>0387-5911</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>10</Month><Day>22</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>10</Month><Day>22</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9780586</ArticleId><ArticleId IdType="doi">10.11150/kansenshogakuzasshi1970.72.827</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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