Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.
Identifieur interne : 002A35 ( Main/Exploration ); précédent : 002A34; suivant : 002A36Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.
Auteurs : F J Bloomfield ; D R Dunstan ; C L Foster ; F. Serafini-Cessi ; R D MarshallSource :
- Biochemical Journal [ 0264-6021 ] ; 1977.
Abstract
Cultured baby-hamster kidney cells (BHK-21/C13), which are adapted to grow in suspension (strain 2P), roduce a glycoprotein, termed BHK glycoprotein I, which cross-reacts immunologically with hamster urinary (Tamm-Horsfall glycoprotein. BHK glycoprotein I was isolated in an electrophoretically (sodium dodecyl sulphate/polyacrylamide gel) homogeneous form by application of affinity chromatography to the medium in which cells had been cultured. Insolubilized anti-(Tamm-Horsfall glycoprotein immunoglobulin G) was used as the adsorbent. The amount of BHK glycoprotein I associated with the cultured cells was found by both radioimmunoassay and immunofluorescence to be related to the amount of Ca2+ in the medium and to the particular stage of the cell cycle. 5'-Nucleotidase was also shed by the cells into the culture medium in amounts related to the stage of the cell cycle. The turnover of hamster Tamm-Horsfall glycoprotein in vivo appeared to be considerably more rapid than can be accounted for by cell turnover. Hamster Tamm-Horsfall glycoprotein was shown to be ineffective in inhibiting agglutination of chicken erythrocytes caused by influenza virus.
Url:
PubMed: 328011
PubMed Central: 1164756
Affiliations:
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Serafini-Cessi</name></author><author><name sortKey="Marshall, R D" sort="Marshall, R D" uniqKey="Marshall R" first="R D" last="Marshall">R D Marshall</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">328011</idno><idno type="pmc">1164756</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1164756</idno><idno type="RBID">PMC:1164756</idno><date when="1977">1977</date><idno type="wicri:Area/Pmc/Corpus">000269</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000269</idno><idno type="wicri:Area/Pmc/Curation">000269</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000269</idno><idno type="wicri:Area/Pmc/Checkpoint">000E08</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000E08</idno><idno type="wicri:Area/Ncbi/Merge">001062</idno><idno type="wicri:Area/Ncbi/Curation">001062</idno><idno type="wicri:Area/Ncbi/Checkpoint">001062</idno><idno type="wicri:doubleKey">0264-6021:1977:Bloomfield F:some:factors:affecting</idno><idno type="wicri:Area/Main/Merge">002B92</idno><idno type="wicri:Area/Main/Curation">002A35</idno><idno type="wicri:Area/Main/Exploration">002A35</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.</title><author><name sortKey="Bloomfield, F J" sort="Bloomfield, F J" uniqKey="Bloomfield F" first="F J" last="Bloomfield">F J Bloomfield</name></author><author><name sortKey="Dunstan, D R" sort="Dunstan, D R" uniqKey="Dunstan D" first="D R" last="Dunstan">D R Dunstan</name></author><author><name sortKey="Foster, C L" sort="Foster, C L" uniqKey="Foster C" first="C L" last="Foster">C L Foster</name></author><author><name sortKey="Serafini Cessi, F" sort="Serafini Cessi, F" uniqKey="Serafini Cessi F" first="F" last="Serafini-Cessi">F. Serafini-Cessi</name></author><author><name sortKey="Marshall, R D" sort="Marshall, R D" uniqKey="Marshall R" first="R D" last="Marshall">R D Marshall</name></author></analytic><series><title level="j">Biochemical Journal</title><idno type="ISSN">0264-6021</idno><idno type="eISSN">1470-8728</idno><imprint><date when="1977">1977</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cultured baby-hamster kidney cells (BHK-21/C13), which are adapted to grow in suspension (strain 2P), roduce a glycoprotein, termed BHK glycoprotein I, which cross-reacts immunologically with hamster urinary (Tamm-Horsfall glycoprotein. BHK glycoprotein I was isolated in an electrophoretically (sodium dodecyl sulphate/polyacrylamide gel) homogeneous form by application of affinity chromatography to the medium in which cells had been cultured. Insolubilized anti-(Tamm-Horsfall glycoprotein immunoglobulin G) was used as the adsorbent. The amount of BHK glycoprotein I associated with the cultured cells was found by both radioimmunoassay and immunofluorescence to be related to the amount of Ca2+ in the medium and to the particular stage of the cell cycle. 5'-Nucleotidase was also shed by the cells into the culture medium in amounts related to the stage of the cell cycle. The turnover of hamster Tamm-Horsfall glycoprotein in vivo appeared to be considerably more rapid than can be accounted for by cell turnover. Hamster Tamm-Horsfall glycoprotein was shown to be ineffective in inhibiting agglutination of chicken erythrocytes caused by influenza virus.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><label>PLATE 1</label><graphic xlink:href="biochemj00510-0062-a" xlink:role="48-1"></graphic></fig><fig id="F2"><label>PLATE 2</label><graphic xlink:href="biochemj00510-0063-a" xlink:role="48-2"></graphic></fig><fig id="F3"><label>PLATE 3</label><graphic xlink:href="biochemj00510-0064-a" xlink:role="48-3"></graphic></fig><fig id="F4"><label>PLATE 4</label><graphic xlink:href="biochemj00510-0065-a" xlink:role="48-4"></graphic></fig></sec></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bloomfield, F J" sort="Bloomfield, F J" uniqKey="Bloomfield F" first="F J" last="Bloomfield">F J Bloomfield</name><name sortKey="Dunstan, D R" sort="Dunstan, D R" uniqKey="Dunstan D" first="D R" last="Dunstan">D R Dunstan</name><name sortKey="Foster, C L" sort="Foster, C L" uniqKey="Foster C" first="C L" last="Foster">C L Foster</name><name sortKey="Marshall, R D" sort="Marshall, R D" uniqKey="Marshall R" first="R D" last="Marshall">R D Marshall</name><name sortKey="Serafini Cessi, F" sort="Serafini Cessi, F" uniqKey="Serafini Cessi F" first="F" last="Serafini-Cessi">F. 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