Bio-nanogate controlled enzymatic reaction for virus sensing.
Identifieur interne : 000587 ( Main/Exploration ); précédent : 000586; suivant : 000588Bio-nanogate controlled enzymatic reaction for virus sensing.
Auteurs : Ronghui Wang [États-Unis] ; Lizhou Xu [République populaire de Chine] ; Yanbin Li [États-Unis]Source :
- Biosensors & bioelectronics [ 1873-4235 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Sous-type H5N1 du virus de la grippe A.
- isolement et purification : Sous-type H5N1 du virus de la grippe A.
- virologie : Grippe chez les oiseaux.
- Animaux, Hémagglutination virale, Limite de détection, Oiseaux, Or, Techniques de biocapteur.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Gold.
- genetics : Influenza A Virus, H5N1 Subtype.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- virology : Influenza in Birds.
- Animals, Biosensing Techniques, Birds, Hemagglutination, Viral, Limit of Detection.
Abstract
The objective of this study was to develop an aptamer-based bifunctional bio-nanogate, which could selectively respond to target molecules, and control enzymatic reaction for electrochemical measurements. It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of other viruses.
DOI: 10.1016/j.bios.2014.08.071
PubMed: 25212377
Affiliations:
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Le document en format XML
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Electronic address: yanbinli@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biosensors & bioelectronics</title><idno type="eISSN">1873-4235</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biosensing Techniques</term><term>Birds</term><term>Gold (chemistry)</term><term>Hemagglutination, Viral</term><term>Influenza A Virus, H5N1 Subtype (genetics)</term><term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term><term>Influenza in Birds (virology)</term><term>Limit of Detection</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Grippe chez les oiseaux (virologie)</term><term>Hémagglutination virale</term><term>Limite de détection</term><term>Oiseaux</term><term>Or ()</term><term>Sous-type H5N1 du virus de la grippe A (génétique)</term><term>Sous-type H5N1 du virus de la grippe A (isolement et purification)</term><term>Techniques de biocapteur</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gold</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe chez les oiseaux</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza in Birds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biosensing Techniques</term><term>Birds</term><term>Hemagglutination, Viral</term><term>Limit of Detection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Hémagglutination virale</term><term>Limite de détection</term><term>Oiseaux</term><term>Or</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of this study was to develop an aptamer-based bifunctional bio-nanogate, which could selectively respond to target molecules, and control enzymatic reaction for electrochemical measurements. It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of other viruses.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></noRegion><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name></country><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Xu, Lizhou" sort="Xu, Lizhou" uniqKey="Xu L" first="Lizhou" last="Xu">Lizhou Xu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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