Bio-nanogate controlled enzymatic reaction for virus sensing.
Identifieur interne : 000077 ( PubMed/Checkpoint ); précédent : 000076; suivant : 000078Bio-nanogate controlled enzymatic reaction for virus sensing.
Auteurs : Ronghui Wang [États-Unis] ; Lizhou Xu [République populaire de Chine] ; Yanbin Li [États-Unis]Source :
- Biosensors & bioelectronics [ 1873-4235 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Sous-type H5N1 du virus de la grippe A.
- isolement et purification : Sous-type H5N1 du virus de la grippe A.
- virologie : Grippe chez les oiseaux.
- Animaux, Hémagglutination virale, Limite de détection, Oiseaux, Or, Techniques de biocapteur.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Gold.
- genetics : Influenza A Virus, H5N1 Subtype.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- virology : Influenza in Birds.
- Animals, Biosensing Techniques, Birds, Hemagglutination, Viral, Limit of Detection.
Abstract
The objective of this study was to develop an aptamer-based bifunctional bio-nanogate, which could selectively respond to target molecules, and control enzymatic reaction for electrochemical measurements. It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of other viruses.
DOI: 10.1016/j.bios.2014.08.071
PubMed: 25212377
Affiliations:
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It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of other viruses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25212377</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>26</Day></DateCompleted><DateRevised><Year>2015</Year><Month>02</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4235</ISSN><JournalIssue CitedMedium="Internet"><Volume>67</Volume><PubDate><Year>2015</Year><Month>May</Month><Day>15</Day></PubDate></JournalIssue><Title>Biosensors & bioelectronics</Title><ISOAbbreviation>Biosens Bioelectron</ISOAbbreviation></Journal><ArticleTitle>Bio-nanogate controlled enzymatic reaction for virus sensing.</ArticleTitle><Pagination><MedlinePgn>400-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bios.2014.08.071</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0956-5663(14)00667-8</ELocationID><Abstract><AbstractText>The objective of this study was to develop an aptamer-based bifunctional bio-nanogate, which could selectively respond to target molecules, and control enzymatic reaction for electrochemical measurements. It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of other viruses.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ronghui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Lizhou</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yanbin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: yanbinli@uark.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>09</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosens Bioelectron</MedlineTA><NlmUniqueID>9001289</NlmUniqueID><ISSNLinking>0956-5663</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance UI="D006046">Gold</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="Y">Biosensing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001717" MajorTopicYN="N">Birds</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006046" MajorTopicYN="N">Gold</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006387" MajorTopicYN="Y">Hemagglutination, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053124" MajorTopicYN="N">Influenza A Virus, H5N1 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005585" MajorTopicYN="N">Influenza in Birds</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aptamer</Keyword><Keyword MajorTopicYN="N">Avian influenza</Keyword><Keyword MajorTopicYN="N">Bio-nanogate</Keyword><Keyword MajorTopicYN="N">Enzymatic reaction</Keyword><Keyword MajorTopicYN="N">Virus sensing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>06</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>08</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>08</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>9</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>9</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25212377</ArticleId><ArticleId IdType="pii">S0956-5663(14)00667-8</ArticleId><ArticleId IdType="doi">10.1016/j.bios.2014.08.071</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></noRegion><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name></country><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Xu, Lizhou" sort="Xu, Lizhou" uniqKey="Xu L" first="Lizhou" last="Xu">Lizhou Xu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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