Influenza virus: association of mouse-lung virulence with plaque formation in mouse kidney cells.
Identifieur interne : 002B04 ( Main/Curation ); précédent : 002B03; suivant : 002B05Influenza virus: association of mouse-lung virulence with plaque formation in mouse kidney cells.
Auteurs : B. Flehmig ; A. Vallbracht ; H J GerthSource :
- Intervirology [ 0300-5526 ] ; 1976.
Descripteurs français
- KwdFr :
- Animaux, Antigènes viraux (analyse), Hémagglutinines virales (analyse), Méthode des plages virales, Poumon (microbiologie), Recombinaison génétique, Réplication virale, Sialidase (immunologie), Souris, Techniques de culture, Virulence, Virus de la grippe A (croissance et développement), Virus de la grippe A (immunologie), Virus de la grippe A (pathogénicité).
- MESH :
- analyse : Antigènes viraux, Hémagglutinines virales.
- croissance et développement : Virus de la grippe A.
- immunologie : Sialidase, Virus de la grippe A.
- microbiologie : Poumon.
- pathogénicité : Virus de la grippe A.
- Animaux, Méthode des plages virales, Recombinaison génétique, Réplication virale, Souris, Techniques de culture, Virulence.
English descriptors
- KwdEn :
- Animals, Antigens, Viral (analysis), Culture Techniques, Hemagglutinins, Viral (analysis), Influenza A virus (growth & development), Influenza A virus (immunology), Influenza A virus (pathogenicity), Lung (microbiology), Mice, Neuraminidase (immunology), Recombination, Genetic, Viral Plaque Assay, Virulence, Virus Replication.
- MESH :
- chemical , analysis : Antigens, Viral, Hemagglutinins, Viral.
- growth & development : Influenza A virus.
- immunology : Influenza A virus, Neuraminidase.
- microbiology : Lung.
- pathogenicity : Influenza A virus.
- Animals, Culture Techniques, Mice, Recombination, Genetic, Viral Plaque Assay, Virulence, Virus Replication.
Abstract
In genetic recombination experiments with the mouse-lung-adapted human influenza A/Engl/1/61 (H2N2) and an avian influenza strain A/Rostock/34 (FPV) (Hav1N1) which is avirulent for the mouse lung, recombinants in which hemagglutinin and neuraminidase were either segregated (Hav1N2; H2N1) or not segregated (Hav1N1) were selected. The recombinants were studied for mouse-lung virulence and their ability to propagate in mouse kidney cells, mouse embryo fibroblasts, chick embryo kidney cells and chick embryo fibroblasts. An association between plaque formation in mouse kidney cells and mouse-lung virulence was found.
DOI: 10.1159/000149953
PubMed: 1010711
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pubmed:1010711Le document en format XML
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<term>Antigens, Viral (analysis)</term>
<term>Culture Techniques</term>
<term>Hemagglutinins, Viral (analysis)</term>
<term>Influenza A virus (growth & development)</term>
<term>Influenza A virus (immunology)</term>
<term>Influenza A virus (pathogenicity)</term>
<term>Lung (microbiology)</term>
<term>Mice</term>
<term>Neuraminidase (immunology)</term>
<term>Recombination, Genetic</term>
<term>Viral Plaque Assay</term>
<term>Virulence</term>
<term>Virus Replication</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antigènes viraux (analyse)</term>
<term>Hémagglutinines virales (analyse)</term>
<term>Méthode des plages virales</term>
<term>Poumon (microbiologie)</term>
<term>Recombinaison génétique</term>
<term>Réplication virale</term>
<term>Sialidase (immunologie)</term>
<term>Souris</term>
<term>Techniques de culture</term>
<term>Virulence</term>
<term>Virus de la grippe A (croissance et développement)</term>
<term>Virus de la grippe A (immunologie)</term>
<term>Virus de la grippe A (pathogénicité)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term>
<term>Hemagglutinins, Viral</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term>
<term>Hémagglutinines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A virus</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sialidase</term>
<term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term>
<term>Neuraminidase</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lung</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A virus</term>
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<term>Culture Techniques</term>
<term>Mice</term>
<term>Recombination, Genetic</term>
<term>Viral Plaque Assay</term>
<term>Virulence</term>
<term>Virus Replication</term>
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<term>Méthode des plages virales</term>
<term>Recombinaison génétique</term>
<term>Réplication virale</term>
<term>Souris</term>
<term>Techniques de culture</term>
<term>Virulence</term>
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<front><div type="abstract" xml:lang="en">In genetic recombination experiments with the mouse-lung-adapted human influenza A/Engl/1/61 (H2N2) and an avian influenza strain A/Rostock/34 (FPV) (Hav1N1) which is avirulent for the mouse lung, recombinants in which hemagglutinin and neuraminidase were either segregated (Hav1N2; H2N1) or not segregated (Hav1N1) were selected. The recombinants were studied for mouse-lung virulence and their ability to propagate in mouse kidney cells, mouse embryo fibroblasts, chick embryo kidney cells and chick embryo fibroblasts. An association between plaque formation in mouse kidney cells and mouse-lung virulence was found.</div>
</front>
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