The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene
Identifieur interne : 000226 ( Istex/Curation ); précédent : 000225; suivant : 000227The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene
Auteurs : G. A. Tannock [Australie] ; J. R. Romanova [Russie] ; J. A. Paul [Australie]Source :
- Archives of Virology [ 0304-8608 ] ; 1995-01-01.
English descriptors
- Teeft :
- Allantoic fluids, Antigenic characteristics, Antiserum, Dos, Equal volume, Haemagglutinin, Haemagglutinin gene, Haemagglutinin genes, Immunogenic properties, Immunogenicity, Infectious centres, Infectious progeny, Influenza, Inoculated, Lower panel, Lung yields, Maintenance medium, Major determinant, Master strain, Mice immunised, Mice inoculated, Mouse model, Neuraminidase, Neuraminidase gene, Parental, Parental strains, Parental virus, Parental viruses, Plaque assays, Reassortant, Reassortant viruses, Reassortants, Relative immunogenicity, Respiratory tract, Royal melbourne institute, Same concentration, Same surface antigens, Segregant, Segregant viruses, Segregants, Significant role, Similar surface antigens, Small volume, Surface antigen gene segregants, Surface antigen segregant, Surface antigen segregants, Surface antigens, Tannock, Upper panel, Vaccine, Virus.
Abstract
Summary: Two surface antigen segregants were prepared by co-infection of chicken embryo kidney cell cultures with reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60-ca (H2N2) possessing the surface antigens of A/Queensland/6/72 (H3N2) and A/Hong Kong/123/77 (H1N1) and other genes that were common to the master strain. The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.
Url:
DOI: 10.1007/BF01309736
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene</title><author><name sortKey="Tannock, G A" sort="Tannock, G A" uniqKey="Tannock G" first="G. A." last="Tannock">G. A. Tannock</name><affiliation wicri:level="1"><mods:affiliation>Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Melbourne, Victoria, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Melbourne, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Romanova, J R" sort="Romanova, J R" uniqKey="Romanova J" first="J. R." last="Romanova">J. R. Romanova</name><affiliation wicri:level="1"><mods:affiliation>Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</mods:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg</wicri:regionArea></affiliation></author><author><name sortKey="Paul, J A" sort="Paul, J A" uniqKey="Paul J" first="J. A." last="Paul">J. A. Paul</name><affiliation wicri:level="1"><mods:affiliation>Faculty of Medicine, The University of Newcastle, Callaghan, New South Wales, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Medicine, The University of Newcastle, Callaghan, New South Wales</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:45EE15EC9972B4E8F5D1926CA29A1FF3BEC813C1</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF01309736</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-SBHPJ1M4-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000226</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000226</idno><idno type="wicri:Area/Istex/Curation">000226</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene</title><author><name sortKey="Tannock, G A" sort="Tannock, G A" uniqKey="Tannock G" first="G. A." last="Tannock">G. A. Tannock</name><affiliation wicri:level="1"><mods:affiliation>Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Melbourne, Victoria, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Melbourne, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Romanova, J R" sort="Romanova, J R" uniqKey="Romanova J" first="J. R." last="Romanova">J. R. Romanova</name><affiliation wicri:level="1"><mods:affiliation>Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</mods:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg</wicri:regionArea></affiliation></author><author><name sortKey="Paul, J A" sort="Paul, J A" uniqKey="Paul J" first="J. A." last="Paul">J. A. Paul</name><affiliation wicri:level="1"><mods:affiliation>Faculty of Medicine, The University of Newcastle, Callaghan, New South Wales, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Medicine, The University of Newcastle, Callaghan, New South Wales</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1995-01-01">1995-01-01</date><biblScope unit="volume">140</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="201">201</biblScope><biblScope unit="page" to="209">209</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Allantoic fluids</term><term>Antigenic characteristics</term><term>Antiserum</term><term>Dos</term><term>Equal volume</term><term>Haemagglutinin</term><term>Haemagglutinin gene</term><term>Haemagglutinin genes</term><term>Immunogenic properties</term><term>Immunogenicity</term><term>Infectious centres</term><term>Infectious progeny</term><term>Influenza</term><term>Inoculated</term><term>Lower panel</term><term>Lung yields</term><term>Maintenance medium</term><term>Major determinant</term><term>Master strain</term><term>Mice immunised</term><term>Mice inoculated</term><term>Mouse model</term><term>Neuraminidase</term><term>Neuraminidase gene</term><term>Parental</term><term>Parental strains</term><term>Parental virus</term><term>Parental viruses</term><term>Plaque assays</term><term>Reassortant</term><term>Reassortant viruses</term><term>Reassortants</term><term>Relative immunogenicity</term><term>Respiratory tract</term><term>Royal melbourne institute</term><term>Same concentration</term><term>Same surface antigens</term><term>Segregant</term><term>Segregant viruses</term><term>Segregants</term><term>Significant role</term><term>Similar surface antigens</term><term>Small volume</term><term>Surface antigen gene segregants</term><term>Surface antigen segregant</term><term>Surface antigen segregants</term><term>Surface antigens</term><term>Tannock</term><term>Upper panel</term><term>Vaccine</term><term>Virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Two surface antigen segregants were prepared by co-infection of chicken embryo kidney cell cultures with reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60-ca (H2N2) possessing the surface antigens of A/Queensland/6/72 (H3N2) and A/Hong Kong/123/77 (H1N1) and other genes that were common to the master strain. The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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