The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene
Identifieur interne : 000226 ( Istex/Corpus ); précédent : 000225; suivant : 000227The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene
Auteurs : G. A. Tannock ; J. R. Romanova ; J. A. PaulSource :
- Archives of Virology [ 0304-8608 ] ; 1995-01-01.
English descriptors
- Teeft :
- Allantoic fluids, Antigenic characteristics, Antiserum, Dos, Equal volume, Haemagglutinin, Haemagglutinin gene, Haemagglutinin genes, Immunogenic properties, Immunogenicity, Infectious centres, Infectious progeny, Influenza, Inoculated, Lower panel, Lung yields, Maintenance medium, Major determinant, Master strain, Mice immunised, Mice inoculated, Mouse model, Neuraminidase, Neuraminidase gene, Parental, Parental strains, Parental virus, Parental viruses, Plaque assays, Reassortant, Reassortant viruses, Reassortants, Relative immunogenicity, Respiratory tract, Royal melbourne institute, Same concentration, Same surface antigens, Segregant, Segregant viruses, Segregants, Significant role, Similar surface antigens, Small volume, Surface antigen gene segregants, Surface antigen segregant, Surface antigen segregants, Surface antigens, Tannock, Upper panel, Vaccine, Virus.
Abstract
Summary: Two surface antigen segregants were prepared by co-infection of chicken embryo kidney cell cultures with reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60-ca (H2N2) possessing the surface antigens of A/Queensland/6/72 (H3N2) and A/Hong Kong/123/77 (H1N1) and other genes that were common to the master strain. The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.
Url:
DOI: 10.1007/BF01309736
Links to Exploration step
ISTEX:45EE15EC9972B4E8F5D1926CA29A1FF3BEC813C1Le document en format XML
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The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. 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A. Tannock</name><affiliations><json:string>Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Melbourne, Victoria, Australia</json:string></affiliations></json:item><json:item><name>J. R. Romanova</name><affiliations><json:string>Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</json:string></affiliations></json:item><json:item><name>J. A. Paul</name><affiliations><json:string>Faculty of Medicine, The University of Newcastle, Callaghan, New South Wales, Australia</json:string></affiliations></json:item></author><articleId><json:string>BF01309736</json:string><json:string>Art19</json:string></articleId><arkIstex>ark:/67375/1BB-SBHPJ1M4-D</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>BriefCommunication</json:string></originalGenre><abstract>Summary: Two surface antigen segregants were prepared by co-infection of chicken embryo kidney cell cultures with reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60-ca (H2N2) possessing the surface antigens of A/Queensland/6/72 (H3N2) and A/Hong Kong/123/77 (H1N1) and other genes that were common to the master strain. The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.</abstract><qualityIndicators><score>7.375</score><pdfWordCount>3143</pdfWordCount><pdfCharCount>19236</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>562 x 774 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>186</abstractWordCount><abstractCharCount>1286</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene</title><pmid><json:string>7646346</json:string></pmid><genre><json:string>brief-communication</json:string></genre><host><title>Archives of Virology</title><language><json:string>unknown</json:string></language><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate><issn><json:string>0304-8608</json:string></issn><eissn><json:string>1432-8798</json:string></eissn><journalId><json:string>705</json:string></journalId><volume>140</volume><issue>1</issue><pages><first>201</first><last>209</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Virology</value></json:item><json:item><value>Infectious Diseases</value></json:item></subject></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/1BB-SBHPJ1M4-D</json:string></ark><categories><wos><json:string>1 - 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The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Virology</term></item><item><term>Infectious Diseases</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1993-09-21">Created</change><change when="1995-01-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-SBHPJ1M4-D/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" 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TocLevels="0"><VolumeIDStart>140</VolumeIDStart><VolumeIDEnd>140</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>21</IssueArticleCount><IssueHistory><CoverDate><DateString>1995</DateString><Year>1995</Year><Month>1</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art19"><ArticleInfo Language="En" ArticleType="BriefCommunication" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF01309736</ArticleID><ArticleDOI>10.1007/BF01309736</ArticleDOI><ArticleSequenceNumber>19</ArticleSequenceNumber><ArticleTitle Language="En">The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene</ArticleTitle><ArticleCategory>Brief Report</ArticleCategory><ArticleFirstPage>201</ArticleFirstPage><ArticleLastPage>209</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>2</Month><Day>28</Day></RegistrationDate><Received><Year>1993</Year><Month>9</Month><Day>21</Day></Received><Accepted><Year>1994</Year><Month>7</Month><Day>21</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>705</JournalID><VolumeIDStart>140</VolumeIDStart><VolumeIDEnd>140</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><GivenName>A.</GivenName><FamilyName>Tannock</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>R.</GivenName><FamilyName>Romanova</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>A.</GivenName><FamilyName>Paul</FamilyName></AuthorName></Author><Affiliation ID="Aff3"><OrgDivision>Faculty of Medicine</OrgDivision><OrgName>The University of Newcastle</OrgName><OrgAddress><City>Callaghan</City><State>New South Wales</State><Country>Australia</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Institute for Experimental Medicine</OrgDivision><OrgName>Russian Academy of Medical Sciences</OrgName><OrgAddress><City>St. Petersburg</City><Country>Russia</Country></OrgAddress></Affiliation><Affiliation ID="Aff1"><OrgDivision>Department of Applied Biology and Biotechnology</OrgDivision><OrgName>Royal Melbourne Institute of Technology</OrgName><OrgAddress><City>Melbourne</City><State>Victoria</State><Country>Australia</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>Two surface antigen segregants were prepared by co-infection of chicken embryo kidney cell cultures with reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60-ca (H2N2) possessing the surface antigens of A/Queensland/6/72 (H3N2) and A/Hong Kong/123/77 (H1N1) and other genes that were common to the master strain. The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The immunogenicity of reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60 is determined by both the genes for cold-adaptation and the haemagglutinin gene</title></titleInfo><name type="personal"><namePart type="given">G.</namePart><namePart type="given">A.</namePart><namePart type="family">Tannock</namePart><affiliation>Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, Melbourne, Victoria, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="given">R.</namePart><namePart type="family">Romanova</namePart><affiliation>Institute for Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="given">A.</namePart><namePart type="family">Paul</namePart><affiliation>Faculty of Medicine, The University of Newcastle, Callaghan, New South Wales, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="BriefCommunication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">1993-09-21</dateCreated><dateIssued encoding="w3cdtf">1995-01-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Summary: Two surface antigen segregants were prepared by co-infection of chicken embryo kidney cell cultures with reassortants of the cold-adapted influenza A master strain A/Ann Arbor/6/60-ca (H2N2) possessing the surface antigens of A/Queensland/6/72 (H3N2) and A/Hong Kong/123/77 (H1N1) and other genes that were common to the master strain. The segregants were shown by serological tests to possess H3N1 and H1N2 surface antigens but it was not possible to determine the presence of H1 or N1 genes by single-stranded RNA polyacrylamide gel electrophoresis. The immunogenic properties of A/Queensland/6/72-ca and H3N1 segregant CR6/35/2/9 were compared by immunising mice intranasally with graded doses of each virus twice at an interval of 3 weeks and then challenging with the wild-type A/Queensland/6/72 (H3N2). Clearance of the challenge virus occurred in mice immunised with the same vaccinating dose, indicating that the immunogenicity of both the ca H3N2 and H3N1 viruses was identical and similar findings were obtained for mice immunised with A/Hong Kong/123/77-ca and the H1N2 segregant CR6/35/1/19 and challenged with the wild-type A/Hong Kong/123/77. Therefore, there appears to be a good correlation between immunogenicity and the inheritance of the haemagglutinin gene.</abstract><note>Brief Report</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title></titleInfo><titleInfo type="abbreviated"><title>Archives of Virology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1995-01-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Infectious Diseases</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">21</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>1995</date><detail type="volume"><number>140</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>201</start><end>209</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1995</recordOrigin></recordInfo></relatedItem><identifier type="istex">45EE15EC9972B4E8F5D1926CA29A1FF3BEC813C1</identifier><identifier type="ark">ark:/67375/1BB-SBHPJ1M4-D</identifier><identifier type="DOI">10.1007/BF01309736</identifier><identifier type="ArticleID">BF01309736</identifier><identifier type="ArticleID">Art19</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1995</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1995</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-SBHPJ1M4-D/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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