Cytotoxic T cells recognize fragments of the influenza nucleoprotein
Identifieur interne : 000F73 ( Istex/Corpus ); précédent : 000F72; suivant : 000F74Cytotoxic T cells recognize fragments of the influenza nucleoprotein
Auteurs : Alain R. M. Townsend ; Frances M. Gotch ; John DaveySource :
- Cell [ 0092-8674 ] ; 1985.
English descriptors
- Teeft :
- Amino, Amino acids, Assay, Cell lines, Cell recognition, Cell surface, Cells cotransfected, Cells transfected, Clone, Cotransfected, Cytotoxic, Davey, Determinant, Epitope, First panel, Fragment, Gene, High expression, Histocompatibility, Immunol, Influenza, Influenza virus, Lymphocyte, Mcmichael, Mmole, Molecular weight, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Mrna, Mrna transcripts, Murine, Nuclear accumulation, Nucleoprotein, Plasma membrane, Plasmid, Polyclonal, Polyclonal cells, Recombinant, Release assay, Same results, Short peptides, Sigma, Skehel, Soluble proteins, Southern analysis, Spleen cells, Townsend, Transfected, Transfected cells, Viral, Viral proteins, Virus, Virus nucleoprotein, Xenopus oocytes, Yewdell.
Abstract
Abstract: Recent work has shown that a major population of murine influenza A specific cytotoxic T lymphocytes (CTL) recognize the viral nucleoprotein. In order to investigate the mechanism by which this nonglycoprotein component of the virus is recognized by CTL, a series of deletion mutants of an A virus NP gene were studied. The results showed that CTL recognize three distinct epitopes of the NP molecule. Both N- and C-terminal fragments of the protein are transported, independently of each other, to the site of recognition by CTL. These findings imply that a mechanism may exist for transport to the cell surface and presentation to CTL, of viral proteins and protein fragments that lack defined signal sequences.
Url:
DOI: 10.1016/0092-8674(85)90103-5
Links to Exploration step
ISTEX:6DCE60103C67668B5E977706D5788E8B1D7E3683Le document en format XML
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Townsend</name><affiliations><json:string>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</json:string></affiliations></json:item><json:item><name>Frances M. Gotch</name><affiliations><json:string>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</json:string></affiliations></json:item><json:item><name>John Davey</name><affiliations><json:string>European Molecular Biology Laboratory Postfach 10.2209 Meyerhofstrasse 1 6900 Heidelberg, Germany</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-QM17956C-7</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Recent work has shown that a major population of murine influenza A specific cytotoxic T lymphocytes (CTL) recognize the viral nucleoprotein. 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These findings imply that a mechanism may exist for transport to the cell surface and presentation to CTL, of viral proteins and protein fragments that lack defined signal sequences.</abstract><qualityIndicators><score>8.392</score><pdfWordCount>7534</pdfWordCount><pdfCharCount>46505</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>576 x 828 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>116</abstractWordCount><abstractCharCount>723</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Cytotoxic T cells recognize fragments of the influenza nucleoprotein</title><pmid><json:string>2411422</json:string></pmid><pii><json:string>0092-8674(85)90103-5</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. 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McMichael</json:string><json:string>John Daveyt</json:string></persName><placeName><json:string>Specificity</json:string><json:string>Lubeck</json:string><json:string>York</json:string><json:string>Cambridge</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Taylor et al., 1970</json:string><json:string>Townsend and Skehel, 1964</json:string><json:string>Maniatis et al. (1982)</json:string><json:string>Zinkernagel and Rosenthal, 1981</json:string><json:string>Townsend et al. (1984a)</json:string><json:string>Davey et al. (1985)</json:string><json:string>Zinkernagel and Doherty, 1979</json:string><json:string>Lubeck et al., 1979</json:string><json:string>McMichael et al., 1980</json:string><json:string>Townsend et al., 1984b</json:string><json:string>Zweerink et al., 1977</json:string><json:string>Virelizier et al., 1977</json:string><json:string>Krieg et al., 1984</json:string><json:string>Ozato and Sachs, 1960</json:string><json:string>Rothbard et al., 1984</json:string><json:string>Bosch et al., 1978</json:string><json:string>reviewed by Goodman and Sercarz, 1983</json:string><json:string>Townsend et al. (1984b)</json:string><json:string>Yewdell et al. (1985)</json:string><json:string>Townsend et al., 1983</json:string><json:string>Unanue et al., 1984</json:string><json:string>Virilizier et al., 1977</json:string><json:string>Chirgwin et al. (1979)</json:string><json:string>Hildreth, 1982</json:string><json:string>Aviv and Leder (1972)</json:string><json:string>Townsend and Skehel(1984)</json:string><json:string>Lancki et al., 1983</json:string><json:string>Bennink et al. (1982)</json:string><json:string>Yewdell et al., 1981</json:string><json:string>Winter and Fields, 1981</json:string><json:string>Townsend et al.. 1984b</json:string><json:string>Townsend and McMichael, 1985</json:string><json:string>Acheson and Mieville, 1978</json:string><json:string>Winter et al., 1981</json:string><json:string>Hood et al., 1985</json:string><json:string>Reddy et al. (1982)</json:string><json:string>Wigler et al. (1978)</json:string><json:string>Simpson, 1984</json:string><json:string>Townsend and Skehel (1984)</json:string><json:string>reviewed by Zinkernagel and Rosenthal, 1981</json:string><json:string>Ciechanover et al., 1984</json:string><json:string>Baez et al., 1980</json:string><json:string>Hershko and Ciechanover, 1982</json:string><json:string>Vitiello and Sherman, 1983</json:string><json:string>Sabatini et al., 1982</json:string><json:string>Townsend et al., 1964b</json:string><json:string>van Wyke et al., 1981</json:string><json:string>Braciale et al., 1984</json:string><json:string>Huddleston and Brownlee, 1982</json:string><json:string>Ey et al. (1978)</json:string><json:string>Askonas et al., 1982</json:string><json:string>Mellor et al., 1982</json:string><json:string>Graham and van der Eb (1973)</json:string><json:string>Flavell et al., 1983</json:string><json:string>Meier-Ewert and Compans, 1974</json:string><json:string>Davey et al., 1985</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-QM17956C-7</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - cell biology</json:string><json:string>2 - biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - General Biochemistry, Genetics and Molecular Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - vertebres: endocrinologie</json:string></inist></categories><publicationDate>1985</publicationDate><copyrightDate>1985</copyrightDate><doi><json:string>10.1016/0092-8674(85)90103-5</json:string></doi><id>6DCE60103C67668B5E977706D5788E8B1D7E3683</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-QM17956C-7/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-QM17956C-7/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-QM17956C-7/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Cytotoxic T cells recognize fragments of the influenza nucleoprotein</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1985</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Cytotoxic T cells recognize fragments of the influenza nucleoprotein</title><author xml:id="author-0000"><persName><forename type="first">Alain R.M.</forename><surname>Townsend</surname></persName><affiliation>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Frances M.</forename><surname>Gotch</surname></persName><affiliation>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</affiliation></author><author xml:id="author-0002"><persName><forename type="first">John</forename><surname>Davey</surname></persName><affiliation>European Molecular Biology Laboratory Postfach 10.2209 Meyerhofstrasse 1 6900 Heidelberg, Germany</affiliation></author><idno type="istex">6DCE60103C67668B5E977706D5788E8B1D7E3683</idno><idno type="ark">ark:/67375/6H6-QM17956C-7</idno><idno type="DOI">10.1016/0092-8674(85)90103-5</idno><idno type="PII">0092-8674(85)90103-5</idno></analytic><monogr><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="pISSN">0092-8674</idno><idno type="PII">S0092-8674(00)X0571-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985"></date><biblScope unit="volume">42</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="457">457</biblScope><biblScope unit="page" to="467">467</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1985</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Recent work has shown that a major population of murine influenza A specific cytotoxic T lymphocytes (CTL) recognize the viral nucleoprotein. In order to investigate the mechanism by which this nonglycoprotein component of the virus is recognized by CTL, a series of deletion mutants of an A virus NP gene were studied. The results showed that CTL recognize three distinct epitopes of the NP molecule. Both N- and C-terminal fragments of the protein are transported, independently of each other, to the site of recognition by CTL. These findings imply that a mechanism may exist for transport to the cell surface and presentation to CTL, of viral proteins and protein fragments that lack defined signal sequences.</p></abstract></profileDesc><revisionDesc><change when="1985-06-25">Modified</change><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-QM17956C-7/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>85901035</aid><ce:pii>0092-8674(85)90103-5</ce:pii><ce:doi>10.1016/0092-8674(85)90103-5</ce:doi><ce:copyright type="unknown" year="1985"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>Cytotoxic T cells recognize fragments of the influenza nucleoprotein</ce:title><ce:author-group><ce:author><ce:given-name>Alain R.M.</ce:given-name><ce:surname>Townsend</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Frances M.</ce:given-name><ce:surname>Gotch</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>John</ce:given-name><ce:surname>Davey</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>European Molecular Biology Laboratory Postfach 10.2209 Meyerhofstrasse 1 6900 Heidelberg, Germany</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="15" month="5" year="1985"></ce:date-received><ce:date-revised day="25" month="6" year="1985"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Recent work has shown that a major population of murine influenza A specific cytotoxic T lymphocytes (CTL) recognize the viral nucleoprotein. In order to investigate the mechanism by which this nonglycoprotein component of the virus is recognized by CTL, a series of deletion mutants of an A virus NP gene were studied. The results showed that CTL recognize three distinct epitopes of the NP molecule. Both N- and C-terminal fragments of the protein are transported, independently of each other, to the site of recognition by CTL. These findings imply that a mechanism may exist for transport to the cell surface and presentation to CTL, of viral proteins and protein fragments that lack defined signal sequences.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Cytotoxic T cells recognize fragments of the influenza nucleoprotein</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Cytotoxic T cells recognize fragments of the influenza nucleoprotein</title></titleInfo><name type="personal"><namePart type="given">Alain R.M.</namePart><namePart type="family">Townsend</namePart><affiliation>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frances M.</namePart><namePart type="family">Gotch</namePart><affiliation>Nuffield Department of Clinical Medicine John Radcliffe Hospital Headington, Oxford, OX3 9DU England</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John</namePart><namePart type="family">Davey</namePart><affiliation>European Molecular Biology Laboratory Postfach 10.2209 Meyerhofstrasse 1 6900 Heidelberg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><dateModified encoding="w3cdtf">1985-06-25</dateModified><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Recent work has shown that a major population of murine influenza A specific cytotoxic T lymphocytes (CTL) recognize the viral nucleoprotein. In order to investigate the mechanism by which this nonglycoprotein component of the virus is recognized by CTL, a series of deletion mutants of an A virus NP gene were studied. The results showed that CTL recognize three distinct epitopes of the NP molecule. Both N- and C-terminal fragments of the protein are transported, independently of each other, to the site of recognition by CTL. These findings imply that a mechanism may exist for transport to the cell surface and presentation to CTL, of viral proteins and protein fragments that lack defined signal sequences.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0571-5</identifier><part><date>1985</date><detail type="volume"><number>42</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>395</start><end>700</end></extent><extent unit="pages"><start>457</start><end>467</end></extent></part></relatedItem><identifier type="istex">6DCE60103C67668B5E977706D5788E8B1D7E3683</identifier><identifier type="ark">ark:/67375/6H6-QM17956C-7</identifier><identifier type="DOI">10.1016/0092-8674(85)90103-5</identifier><identifier type="PII">0092-8674(85)90103-5</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-QM17956C-7/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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