Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”
Identifieur interne : 000C59 ( Istex/Corpus ); précédent : 000C58; suivant : 000C60Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”
Auteurs : Emmanuelle Trévisiol ; Eric Defrancq ; Jean Lhomme ; Ali Laayoun ; Philippe CrosSource :
- European Journal of Organic Chemistry [ 1434-193X ] ; 2000-01.
English descriptors
- Teeft :
- Acidic conditions, Aldehydic, Aldehydic fluorophore, Aminooxy, Aminooxy function, Anhydrous, Anhydrous conditions, Anhydrous pyridine, Calcd, Cdcl3, Chem, Crude product, Deprotected nucleoside, Ether, Ethyl orthoformate, Fluorescein, Fluorescent label, Fluorophore, Full paper, Hrms, Isobutyl chloroformate, Labelling, Matrix, Methoxyamine hydrochloride, Mmol, Mnba, Mnba matrix, Nucleoside, Nucleoside triphosphates, Nucleotide, Oily residue, Orange powder, Organic layer, Oxime, Oxime ether, Pyridine, Quat, Reaction mixture, Room temp, Room temperature, Silica, Temp, Tetrahedron lett, Transcription products, Tributylammonium pyrophosphate, Triphosphate, Triphosphates, Tris buffer, Uridine, White powder.
Abstract
Preparation of the uridine and adenosine triphosphates 1 and 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. They could each be incorporated into a 330‐mer fragment with T7 RNA polymerase.
Url:
DOI: 10.1002/(SICI)1099-0690(200001)2000:1<211::AID-EJOC211>3.0.CO;2-V
Links to Exploration step
ISTEX:9503F72893DD632AEF4F5F4F1CC1BA06E677A549Le document en format XML
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Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. 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Ruffieux</json:string><json:string>E. Trevisiol</json:string><json:string>H. Ide</json:string><json:string>D. Boturyn</json:string><json:string>P. M. Kaplan</json:string><json:string>P. Vincent</json:string><json:string>E. Defrancq</json:string><json:string>S. Desvarenne</json:string><json:string>M. J. Robins</json:string><json:string>Preparation</json:string><json:string>A. Troesch</json:string><json:string>K. J. M. Moore</json:string><json:string>S. Pochet</json:string><json:string>M. Kajtar</json:string><json:string>J. Clin</json:string><json:string>C. R. Cremo</json:string><json:string>C. G. Miyada</json:string><json:string>C. Guerreiro</json:string><json:string>J. Garcia</json:string><json:string>S. R. Sarfati</json:string><json:string>K. Makino</json:string><json:string>J. Hodgson</json:string><json:string>C. Kessler</json:string><json:string>R. P. Haugland</json:string><json:string>V. A. Korshun</json:string><json:string>Y. A. Berlin</json:string><json:string>A. 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Rep. of Germany</copyright><eventGroup><event type="manuscriptReceived" date="1999-05-03"></event><event type="firstOnline" date="1999-12-13"></event><event type="publishedOnlineFinalForm" date="1999-12-13"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-06"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-24"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst">211</numbering><numbering type="pageLast">217</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:EJOC.EJOC211.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="13"></count></countGroup><titleGroup><title type="main" xml:lang="en">Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Emmanuelle</givenNames><familyName>Trévisiol</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Eric</givenNames><familyName>Defrancq</familyName></personName></creator><creator xml:id="au3" creatorRole="author"><personName><givenNames>Jean</givenNames><familyName>Lhomme</familyName></personName><contactDetails><email normalForm="Jean.Lhomme@ujf-grenoble.fr">Jean.Lhomme@ujf‐grenoble.fr</email></contactDetails></creator><creator xml:id="au4" creatorRole="author"><personName><givenNames>Ali</givenNames><familyName>Laayoun</familyName></personName><contactDetails><email>alaayoun@ml.biomerieux.fr</email></contactDetails></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#a2"><personName><givenNames>Philippe</givenNames><familyName>Cros</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR" type="organization"><unparsedAffiliation>LEDSS, Chimie Bioorganique, UMR CNRS 5616, Université Joseph Fourier, B. 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They could each be incorporated into a 330‐mer fragment with T7 RNA polymerase.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”</title></titleInfo><name type="personal"><namePart type="given">Emmanuelle</namePart><namePart type="family">Trévisiol</namePart><affiliation>LEDSS, Chimie Bioorganique, UMR CNRS 5616, Université Joseph Fourier, B. P. 53, F‐38041 Grenoble Cedex 9, France Fax: (internat.) + 33‐4/76514382</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eric</namePart><namePart type="family">Defrancq</namePart><affiliation>LEDSS, Chimie Bioorganique, UMR CNRS 5616, Université Joseph Fourier, B. P. 53, F‐38041 Grenoble Cedex 9, France Fax: (internat.) + 33‐4/76514382</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean</namePart><namePart type="family">Lhomme</namePart><affiliation>E-mail: Jean.Lhomme@ujf‐grenoble.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ali</namePart><namePart type="family">Laayoun</namePart><affiliation>E-mail: alaayoun@ml.biomerieux.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Philippe</namePart><namePart type="family">Cros</namePart><affiliation>BioMérieux, Laboratoire Sondes Nucléiques, Chemin de ld'Orme, F‐69280 Marcy‐l'Etoile, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>WILEY‐VCH Verlag GmbH</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2000-01</dateIssued><dateCaptured encoding="w3cdtf">1999-05-03</dateCaptured><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">2</extent><extent unit="tables">0</extent><extent unit="references">13</extent></physicalDescription><abstract lang="en">Preparation of the uridine and adenosine triphosphates 1 and 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. They could each be incorporated into a 330‐mer fragment with T7 RNA polymerase.</abstract><subject lang="en"><genre>keywords</genre><topic>Aminooxy group</topic><topic>Phosphorylations</topic><topic>Nucleotides</topic><topic>Fluorescent labelling</topic></subject><relatedItem type="host"><titleInfo><title>European Journal of Organic Chemistry</title></titleInfo><titleInfo type="abbreviated"><title>Eur. J. Org. 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