Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response
Identifieur interne : 000C15 ( Istex/Corpus ); précédent : 000C14; suivant : 000C16Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response
Auteurs : Takashi Tomoda ; Hideo Morita ; Takanobu Kurashige ; Hunein F. MaassabSource :
- Vaccine [ 0264-410X ] ; 1995.
English descriptors
- Teeft :
- Antibody, Antibody response, Cultured lymphocytes, Examinee, Healthy adults, Hlnl, Hlnl virus, Immunological factors, Important role, Important roles, Influenza, Influenza cytotoxic, Influenza epidemics, Influenza infection, Influenza vaccine, Influenza virus, Influenza virus vaccine, Intranasal administration, Kochi, Kochi prefecture, Lower morbidity rate, Lymphocyte, Lymphocyte activation, Lymphocyte proliferation, Lymphocyte proliferation assay, Nasal, Nasal wash specimen, Nasal wash specimens, Questionnaire survey, Saliva samples, Second vaccination, Significant change, Significant increase, Stimulation index, Untreated groups, Untreated ones, Vaccinated, Vaccinated examinees, Vaccinated individuals, Vaccinated students, Vaccination, Vaccine, Virus, Virus infection.
Abstract
Abstract: To clarify which immunological factors were more effective in preventing influenza virus infection, we measured immunological parameters induced by vaccination and infection in vivo and in vitro. Healthy adult subjects (n = 128) were divided into vaccinated (n = 85) and untreated (n = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus in vitro were correlated with the HI antibody titre. However, the interferon γ (IFN-γ) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-γ production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6–8 weeks after the second vaccination, we examined the above hypothesis. A significantly (p<0.01) lower morbidity rate in the vaccinated examinees was found. The HI antibody, lymphocyte DNA synthesis and IL-2 production, which had been increased by vaccination, showed little further increase in response to the epidemic. In contrast, local IgA response and IFN-γ production, which had been increased by vaccination, showed another increase because of the epidemc.
Url:
DOI: 10.1016/0264-410X(95)93134-U
Links to Exploration step
ISTEX:A29E5FF5D88DFDA97C91FC301EC44CFA1A60B16ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response</title><author><name sortKey="Tomoda, Takashi" sort="Tomoda, Takashi" uniqKey="Tomoda T" first="Takashi" last="Tomoda">Takashi Tomoda</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author><author><name sortKey="Morita, Hideo" sort="Morita, Hideo" uniqKey="Morita H" first="Hideo" last="Morita">Hideo Morita</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author><author><name sortKey="Kurashige, Takanobu" sort="Kurashige, Takanobu" uniqKey="Kurashige T" first="Takanobu" last="Kurashige">Takanobu Kurashige</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author><author><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A29E5FF5D88DFDA97C91FC301EC44CFA1A60B16E</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/0264-410X(95)93134-U</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-X7L6SPWB-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C15</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C15</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response</title><author><name sortKey="Tomoda, Takashi" sort="Tomoda, Takashi" uniqKey="Tomoda T" first="Takashi" last="Tomoda">Takashi Tomoda</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author><author><name sortKey="Morita, Hideo" sort="Morita, Hideo" uniqKey="Morita H" first="Hideo" last="Morita">Hideo Morita</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author><author><name sortKey="Kurashige, Takanobu" sort="Kurashige, Takanobu" uniqKey="Kurashige T" first="Takanobu" last="Kurashige">Takanobu Kurashige</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author><author><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><affiliation><mods:affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">13</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="190">190</biblScope></imprint><idno type="ISSN">0264-410X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antibody</term><term>Antibody response</term><term>Cultured lymphocytes</term><term>Examinee</term><term>Healthy adults</term><term>Hlnl</term><term>Hlnl virus</term><term>Immunological factors</term><term>Important role</term><term>Important roles</term><term>Influenza</term><term>Influenza cytotoxic</term><term>Influenza epidemics</term><term>Influenza infection</term><term>Influenza vaccine</term><term>Influenza virus</term><term>Influenza virus vaccine</term><term>Intranasal administration</term><term>Kochi</term><term>Kochi prefecture</term><term>Lower morbidity rate</term><term>Lymphocyte</term><term>Lymphocyte activation</term><term>Lymphocyte proliferation</term><term>Lymphocyte proliferation assay</term><term>Nasal</term><term>Nasal wash specimen</term><term>Nasal wash specimens</term><term>Questionnaire survey</term><term>Saliva samples</term><term>Second vaccination</term><term>Significant change</term><term>Significant increase</term><term>Stimulation index</term><term>Untreated groups</term><term>Untreated ones</term><term>Vaccinated</term><term>Vaccinated examinees</term><term>Vaccinated individuals</term><term>Vaccinated students</term><term>Vaccination</term><term>Vaccine</term><term>Virus</term><term>Virus infection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: To clarify which immunological factors were more effective in preventing influenza virus infection, we measured immunological parameters induced by vaccination and infection in vivo and in vitro. Healthy adult subjects (n = 128) were divided into vaccinated (n = 85) and untreated (n = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus in vitro were correlated with the HI antibody titre. However, the interferon γ (IFN-γ) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-γ production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6–8 weeks after the second vaccination, we examined the above hypothesis. A significantly (p<0.01) lower morbidity rate in the vaccinated examinees was found. The HI antibody, lymphocyte DNA synthesis and IL-2 production, which had been increased by vaccination, showed little further increase in response to the epidemic. In contrast, local IgA response and IFN-γ production, which had been increased by vaccination, showed another increase because of the epidemc.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>lymphocyte</json:string><json:string>influenza</json:string><json:string>vaccinated</json:string><json:string>vaccine</json:string><json:string>hlnl</json:string><json:string>vaccination</json:string><json:string>examinee</json:string><json:string>influenza virus</json:string><json:string>kochi</json:string><json:string>influenza vaccine</json:string><json:string>intranasal administration</json:string><json:string>kochi prefecture</json:string><json:string>nasal wash specimens</json:string><json:string>vaccinated individuals</json:string><json:string>influenza virus vaccine</json:string><json:string>second vaccination</json:string><json:string>lower morbidity rate</json:string><json:string>nasal wash specimen</json:string><json:string>hlnl virus</json:string><json:string>stimulation index</json:string><json:string>lymphocyte activation</json:string><json:string>virus infection</json:string><json:string>nasal</json:string><json:string>antibody</json:string><json:string>saliva samples</json:string><json:string>influenza infection</json:string><json:string>healthy adults</json:string><json:string>vaccinated students</json:string><json:string>untreated ones</json:string><json:string>important roles</json:string><json:string>questionnaire survey</json:string><json:string>cultured lymphocytes</json:string><json:string>lymphocyte proliferation assay</json:string><json:string>significant change</json:string><json:string>antibody response</json:string><json:string>vaccinated examinees</json:string><json:string>significant increase</json:string><json:string>lymphocyte proliferation</json:string><json:string>untreated groups</json:string><json:string>immunological factors</json:string><json:string>important role</json:string><json:string>influenza epidemics</json:string><json:string>influenza cytotoxic</json:string><json:string>virus</json:string></teeft></keywords><author><json:item><name>Takashi Tomoda</name><affiliations><json:string>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</json:string></affiliations></json:item><json:item><name>Hideo Morita</name><affiliations><json:string>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</json:string></affiliations></json:item><json:item><name>Takanobu Kurashige</name><affiliations><json:string>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</json:string></affiliations></json:item><json:item><name>Hunein F. Maassab</name><affiliations><json:string>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Cold-recombinant</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>live attenuated</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>influenza vaccine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>intranasal administration</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>interferon-γ</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>local IgA</value></json:item></subject><arkIstex>ark:/67375/6H6-X7L6SPWB-S</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: To clarify which immunological factors were more effective in preventing influenza virus infection, we measured immunological parameters induced by vaccination and infection in vivo and in vitro. Healthy adult subjects (n = 128) were divided into vaccinated (n = 85) and untreated (n = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus in vitro were correlated with the HI antibody titre. However, the interferon γ (IFN-γ) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-γ production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6–8 weeks after the second vaccination, we examined the above hypothesis. A significantly (p>0.01) lower morbidity rate in the vaccinated examinees was found. The HI antibody, lymphocyte DNA synthesis and IL-2 production, which had been increased by vaccination, showed little further increase in response to the epidemic. 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Healthy adult subjects (n = 128) were divided into vaccinated (n = 85) and untreated (n = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus in vitro were correlated with the HI antibody titre. However, the interferon γ (IFN-γ) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-γ production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6–8 weeks after the second vaccination, we examined the above hypothesis. A significantly (p<0.01) lower morbidity rate in the vaccinated examinees was found. The HI antibody, lymphocyte DNA synthesis and IL-2 production, which had been increased by vaccination, showed little further increase in response to the epidemic. In contrast, local IgA response and IFN-γ production, which had been increased by vaccination, showed another increase because of the epidemc.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Cold-recombinant</term></item><item><term>live attenuated</term></item><item><term>influenza vaccine</term></item><item><term>intranasal administration</term></item><item><term>interferon-γ</term></item><item><term>local IgA</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994-06-17">Modified</change><change when="1995">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-X7L6SPWB-S/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JVAC</jid><aid>9593134U</aid><ce:pii>0264-410X(95)93134-U</ce:pii><ce:doi>10.1016/0264-410X(95)93134-U</ce:doi><ce:copyright type="unknown" year="1995"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Paper</ce:textfn></ce:dochead><ce:title>Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response</ce:title><ce:author-group><ce:author><ce:given-name>Takashi</ce:given-name><ce:surname>Tomoda</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Hideo</ce:given-name><ce:surname>Morita</ce:surname></ce:author><ce:author><ce:given-name>Takanobu</ce:given-name><ce:surname>Kurashige</ce:surname></ce:author><ce:author><ce:given-name>Hunein F.</ce:given-name><ce:surname>Maassab</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>To whom correspondence should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="7" month="4" year="1994"></ce:date-received><ce:date-revised day="17" month="6" year="1994"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para id="simple-para0005">To clarify which immunological factors were more effective in preventing influenza virus infection, we measured immunological parameters induced by vaccination and infection <ce:italic>in vivo</ce:italic> and <ce:italic>in vitro</ce:italic>. Healthy adult subjects (<ce:italic>n</ce:italic> = 128) were divided into vaccinated (<ce:italic>n</ce:italic> = 85) and untreated (<ce:italic>n</ce:italic> = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus <ce:italic>in vitro</ce:italic> were correlated with the HI antibody titre. However, the interferon γ (IFN-γ) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-γ production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6–8 weeks after the second vaccination, we examined the above hypothesis. A significantly (<ce:italic>p</ce:italic><0.01) lower morbidity rate in the vaccinated examinees was found. The HI antibody, lymphocyte DNA synthesis and IL-2 production, which had been increased by vaccination, showed little further increase in response to the epidemic. In contrast, local IgA response and IFN-γ production, which had been increased by vaccination, showed another increase because of the epidemc.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Cold-recombinant</ce:text></ce:keyword><ce:keyword><ce:text>live attenuated</ce:text></ce:keyword><ce:keyword><ce:text>influenza vaccine</ce:text></ce:keyword><ce:keyword><ce:text>intranasal administration</ce:text></ce:keyword><ce:keyword><ce:text>interferon-γ</ce:text></ce:keyword><ce:keyword><ce:text>local IgA</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Prevention of influenza by the intranasal administration of cold-recombinant, live-attenuated influenza virus vaccine: importance of interferon-γ production and local IgA response</title></titleInfo><name type="personal"><namePart type="given">Takashi</namePart><namePart type="family">Tomoda</namePart><affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</affiliation><description>To whom correspondence should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hideo</namePart><namePart type="family">Morita</namePart><affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Takanobu</namePart><namePart type="family">Kurashige</namePart><affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hunein F.</namePart><namePart type="family">Maassab</namePart><affiliation>Department of Pediatrics, Kochi Medical School, Okoh-cho Nankoku, Kochi, 783 Japan and Department of Epidemiology, School of Public Health, The University of Michigan, Ann Arbor, MI 48109, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><dateModified encoding="w3cdtf">1994-06-17</dateModified><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: To clarify which immunological factors were more effective in preventing influenza virus infection, we measured immunological parameters induced by vaccination and infection in vivo and in vitro. Healthy adult subjects (n = 128) were divided into vaccinated (n = 85) and untreated (n = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus in vitro were correlated with the HI antibody titre. However, the interferon γ (IFN-γ) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-γ production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6–8 weeks after the second vaccination, we examined the above hypothesis. A significantly (p<0.01) lower morbidity rate in the vaccinated examinees was found. The HI antibody, lymphocyte DNA synthesis and IL-2 production, which had been increased by vaccination, showed little further increase in response to the epidemic. In contrast, local IgA response and IFN-γ production, which had been increased by vaccination, showed another increase because of the epidemc.</abstract><note type="content">Section title: Paper</note><subject><genre>Keywords</genre><topic>Cold-recombinant</topic><topic>live attenuated</topic><topic>influenza vaccine</topic><topic>intranasal administration</topic><topic>interferon-γ</topic><topic>local IgA</topic></subject><relatedItem type="host"><titleInfo><title>Vaccine</title></titleInfo><titleInfo type="abbreviated"><title>JVAC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued></originInfo><identifier type="ISSN">0264-410X</identifier><identifier type="PII">S0264-410X(00)X0028-X</identifier><part><date>1995</date><detail type="volume"><number>13</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>139</start><end>232</end></extent><extent unit="pages"><start>185</start><end>190</end></extent></part></relatedItem><identifier type="istex">A29E5FF5D88DFDA97C91FC301EC44CFA1A60B16E</identifier><identifier type="ark">ark:/67375/6H6-X7L6SPWB-S</identifier><identifier type="DOI">10.1016/0264-410X(95)93134-U</identifier><identifier type="PII">0264-410X(95)93134-U</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-X7L6SPWB-S/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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