Attenuation and Phenotypic Stability of Influenza B/Texas/l/84 Cold-Adapted Reassortant Virus: Studies in Hamsters and Chimpanzees
Identifieur interne : 000930 ( Istex/Corpus ); précédent : 000929; suivant : 000931Attenuation and Phenotypic Stability of Influenza B/Texas/l/84 Cold-Adapted Reassortant Virus: Studies in Hamsters and Chimpanzees
Auteurs : Mark H. Snyder ; William T. London ; Hunein F. Maassab ; Brian R. MurphySource :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1989.
Abstract
The temperature sensitivity, level of attenuation, and phenotypic stability of an influenza B/Texas/1/84 x B/Ann Arbor/1/66 cold-adapted (ca) reassortant virus that received the RNA segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the B/Texas/84 wild-type virus and the remaining six RNA segments from the B/Ann Arbor/66 ca donor virus were evaluated. In comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 ca donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the ca virus retained the temperature-sensitive (ts) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the ca reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their ts and attenuation phenotypes even after prolonged replication in vivo. Thus, the B/Ann Arbor/66 ca donor virus can transfer the desired properties of attenuation and phenotype stability to its reassortants. The findings support the continued evaluation of ca reassortant influenza B virus vaccines in humans, including fully susceptible children.
Url:
DOI: 10.1093/infdis/160.4.604
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Snyder</name><affiliation><mods:affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</mods:affiliation></affiliation></author><author><name sortKey="London, William T" sort="London, William T" uniqKey="London W" first="William T." last="London">William T. London</name><affiliation><mods:affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</mods:affiliation></affiliation></author><author><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><affiliation><mods:affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</mods:affiliation></affiliation></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><affiliation><mods:affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</mods:affiliation></affiliation><affiliation><mods:affiliation>Please address requests for reprints to Dr. Brian R. Murphy, Laboratory of Infectious Diseases, Building 7, Room 106, Bethesda, MD 20892.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Infectious Diseases</title><title level="j" type="abbrev">J Infect Dis.</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date when="1989-10">1989</date><biblScope unit="vol">160</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="604">604</biblScope><biblScope unit="page" to="610">610</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The temperature sensitivity, level of attenuation, and phenotypic stability of an influenza B/Texas/1/84 x B/Ann Arbor/1/66 cold-adapted (ca) reassortant virus that received the RNA segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the B/Texas/84 wild-type virus and the remaining six RNA segments from the B/Ann Arbor/66 ca donor virus were evaluated. In comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 ca donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the ca virus retained the temperature-sensitive (ts) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the ca reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their ts and attenuation phenotypes even after prolonged replication in vivo. 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In comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 ca donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the ca virus retained the temperature-sensitive (ts) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the ca reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their ts and attenuation phenotypes even after prolonged replication in vivo. 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<surname>Snyder</surname><given-names>Mark H.</given-names>
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<surname>London</surname><given-names>William T.</given-names>
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</contrib>
<aff><institution>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases</institution>, <addr-line>Bethesda, Maryland</addr-line></aff>
<aff><institution>From the National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health</institution>, <addr-line>Bethesda, Maryland</addr-line></aff>
<aff><institution>From the Department of Epidemiology, School of Public Health, University of Michigan</institution>, <addr-line>Ann Arbor</addr-line></aff>
</contrib-group>
<author-notes>
<corresp id="COR1"><label>1</label>Please address requests for reprints to Dr. Brian R. Murphy, Laboratory of Infectious Diseases, Building 7, Room 106, Bethesda, MD 20892.</corresp>
</author-notes>
<pub-date pub-type="ppub">
<month>10</month>
<year>1989</year>
</pub-date>
<volume>160</volume>
<issue>4</issue>
<fpage>604</fpage>
<lpage>610</lpage>
<history>
<date date-type="received">
<day>30</day>
<month>11</month>
<year>1988</year>
</date>
<date date-type="rev-recd">
<day>17</day>
<month>5</month>
<year>1989</year>
</date>
</history>
<copyright-statement>© 1989 by The University of Chicago</copyright-statement>
<copyright-year>1989</copyright-year>
<abstract>
<p>The temperature sensitivity, level of attenuation, and phenotypic stability of an influenza B/Texas/1/84 x B/Ann Arbor/1/66 cold-adapted (<italic>ca</italic>) reassortant virus that received the RNA segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the B/Texas/84 wild-type virus and the remaining six RNA segments from the B/Ann Arbor/66 <italic>ca</italic> donor virus were evaluated. In comparison to wild-type virus, the <italic>ca</italic> reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 <italic>ca</italic> donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the <italic>ca</italic> virus retained the temperature-sensitive (<italic>ts</italic>) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the <italic>ca</italic> reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their <italic>ts</italic> and attenuation phenotypes even after prolonged replication in vivo. Thus, the B/Ann Arbor/66 <italic>ca</italic> donor virus can transfer the desired properties of attenuation and phenotype stability to its reassortants. The findings support the continued evaluation of <italic>ca</italic> reassortant influenza B virus vaccines in humans, including fully susceptible children.</p>
</abstract>
</article-meta>
</front>
</article>
</istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Attenuation and Phenotypic Stability of Influenza B/Texas/l/84 Cold-Adapted Reassortant Virus: Studies in Hamsters and Chimpanzees</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Attenuation and Phenotypic Stability of Influenza B/Texas/l/84 Cold-Adapted Reassortant Virus: Studies in Hamsters and Chimpanzees</title></titleInfo><name type="personal"><namePart type="given">Mark H.</namePart><namePart type="family">Snyder</namePart><affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William T.</namePart><namePart type="family">London</namePart><affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hunein F.</namePart><namePart type="family">Maassab</namePart><affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Brian R.</namePart><namePart type="family">Murphy</namePart><affiliation>From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, Bethesda, Maryland, Ann Arbor</affiliation><affiliation>Please address requests for reprints to Dr. Brian R. Murphy, Laboratory of Infectious Diseases, Building 7, Room 106, Bethesda, MD 20892.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>The University of Chicago Press</publisher><dateIssued encoding="w3cdtf">1989-10</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><abstract>The temperature sensitivity, level of attenuation, and phenotypic stability of an influenza B/Texas/1/84 x B/Ann Arbor/1/66 cold-adapted (ca) reassortant virus that received the RNA segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the B/Texas/84 wild-type virus and the remaining six RNA segments from the B/Ann Arbor/66 ca donor virus were evaluated. In comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respiratory tracts of hamsters and chimpanzees. This clearly demonstrated that the six transferable RNA segments of the B/Ann Arbor/66 ca donor virus specify the attenuation phenotype for animals with a 37°C core body temperature. In addition, the ca virus retained the temperature-sensitive (ts) phenotype after replication in hamsters and chimpanzees. To further evaluate its phenotypic stability, the ca reassortant virus was isolated after 6-15 d of replication in hamsters that were immunosuppressed with cyclophosphamide, and the isolates were tested for their temperature sensitivity in vitro and for their level of replication in immunocompetent hamsters. The isolated viruses retained their ts and attenuation phenotypes even after prolonged replication in vivo. Thus, the B/Ann Arbor/66 ca donor virus can transfer the desired properties of attenuation and phenotype stability to its reassortants. The findings support the continued evaluation of ca reassortant influenza B virus vaccines in humans, including fully susceptible children.</abstract><note type="author-notes">1Please address requests for reprints to Dr. Brian R. Murphy, Laboratory of Infectious Diseases, Building 7, Room 106, Bethesda, MD 20892.</note><relatedItem type="host"><titleInfo><title>Journal of Infectious Diseases</title></titleInfo><titleInfo type="abbreviated"><title>J Infect Dis.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0022-1899</identifier><identifier type="eISSN">1537-6613</identifier><identifier type="PublisherID">jid</identifier><identifier type="PublisherID-hwp">jinfdis</identifier><part><date>1989</date><detail type="volume"><caption>vol.</caption><number>160</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>604</start><end>610</end></extent></part></relatedItem><identifier type="istex">9EE908783F57A9B3A9BC709ABA51307D1AA5BBB8</identifier><identifier type="ark">ark:/67375/HXZ-3M5HK0TX-V</identifier><identifier type="DOI">10.1093/infdis/160.4.604</identifier><accessCondition type="use and reproduction" contentType="copyright">© 1989 by The University of Chicago</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-GTWS0RDP-M">oup</recordContentSource><recordOrigin>Converted from (version 1.2.0) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-12-09</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/HXZ-3M5HK0TX-V/record.json</uri></json:item></metadata><covers><json:item><extension>tiff</extension><original>true</original><mimetype>image/tiff</mimetype><uri>https://api.istex.fr/document/9EE908783F57A9B3A9BC709ABA51307D1AA5BBB8/covers/tiff</uri></json:item></covers><annexes><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/HXZ-3M5HK0TX-V/annexes.pdf</uri></json:item></annexes><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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