H2N2V1, Hal, Checkpoint, bibRecord, 000287

Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

Identifieur interne : 000287 ( Hal/Checkpoint ); précédent : 000286; suivant : 000288

Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

Auteurs : Peter P. Cheung [République populaire de Chine] ; Y H Connie Leung [République populaire de Chine] ; Chun-Kin Chow [République populaire de Chine] ; Chi-Fung Ng [République populaire de Chine] ; Chun-Lok Tsang [République populaire de Chine] ; Yu-On Wu [République populaire de Chine] ; Siu-Kit Ma [République populaire de Chine] ; Sin-Fun Sia [République populaire de Chine] ; Yi Guan [République populaire de Chine] ; J. S. M. Peiris [République populaire de Chine]

Source :

Journal of Clinical Virology [ 1386-6532 ] ; 2009-09.

RBID : Hal:pasteur-00588920

Abstract

BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-00588920

DOI: 10.1016/j.jcv.2009.06.016

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000143
to stream Hal, to step Curation: 000143

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Hal:pasteur-00588920

Le document en format XML

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<author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-136023" status="VALID">          <orgName>Department of Microbiology [HKU]</orgName>
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<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-136023" status="VALID">          <orgName>Department of Microbiology [HKU]</orgName>
          <desc>            <address>              <addrLine>Hong Kong Special Administrative Region, China</addrLine>
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          <desc>            <address>              <addrLine>Pokfulam, Hong Kong</addrLine>
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<country>République populaire de Chine</country>
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<idno type="DOI">10.1016/j.jcv.2009.06.016</idno>
<series><title level="j">Journal of Clinical Virology</title>
<idno type="ISSN">1386-6532</idno>
<imprint><date type="datePub">2009-09</date>
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<front><div type="abstract" xml:lang="en">              <p>BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.</p>
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            <author role="aut">              <persName>                <forename type="first">Peter P</forename>
                <surname>Cheung</surname>
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            <author role="aut">              <persName>                <forename type="first">Y H Connie</forename>
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            <author role="aut">              <persName>                <forename type="first">Yu-On</forename>
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            <author role="aut">              <persName>                <forename type="first">Siu-Kit</forename>
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            <author role="aut">              <persName>                <forename type="first">Sin-Fun</forename>
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          <sourceDesc>            <biblStruct>              <analytic>                <title xml:lang="en">Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.</title>
                <author role="aut">                  <persName>                    <forename type="first">Peter P</forename>
                    <surname>Cheung</surname>
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                  <idno type="halauthorid">603601</idno>
                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Y H Connie</forename>
                    <surname>Leung</surname>
                  </persName>
                  <idno type="halauthorid">603602</idno>
                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Chun-Kin</forename>
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                  <affiliation ref="#struct-136023"></affiliation>
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                <author role="aut">                  <persName>                    <forename type="first">Chi-Fung</forename>
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                  <affiliation ref="#struct-136023"></affiliation>
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                <author role="aut">                  <persName>                    <forename type="first">Chun-Lok</forename>
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                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Yu-On</forename>
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                  <idno type="halauthorid">603606</idno>
                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Siu-Kit</forename>
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                  <idno type="halauthorid">603607</idno>
                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Sin-Fun</forename>
                    <surname>Sia</surname>
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                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="aut">                  <persName>                    <forename type="first">Yi</forename>
                    <surname>Guan</surname>
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                  <idno type="halauthorid">603537</idno>
                  <affiliation ref="#struct-136023"></affiliation>
                </author>
                <author role="crp">                  <persName>                    <forename type="first">J. S. M.</forename>
                    <surname>Peiris</surname>
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                  <email type="md5">cc6ecab6abc0bb33758973d51e488f7b</email>
                  <email type="domain">hkucc.hku.hk</email>
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                <idno type="issn">1386-6532</idno>
                <title level="j">Journal of Clinical Virology</title>
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                  <date type="datePub">2009-09</date>
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            <abstract xml:lang="en">              <p>BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.</p>
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Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

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