Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.
Identifieur interne : 000143 ( Hal/Corpus ); précédent : 000142; suivant : 000144Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.
Auteurs : Peter P. Cheung ; Y H Connie Leung ; Chun-Kin Chow ; Chi-Fung Ng ; Chun-Lok Tsang ; Yu-On Wu ; Siu-Kit Ma ; Sin-Fun Sia ; Yi Guan ; J. S. M. PeirisSource :
- Journal of Clinical Virology [ 1386-6532 ] ; 2009-09.
Abstract
BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.
Url:
DOI: 10.1016/j.jcv.2009.06.016
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Hal:pasteur-00588920Le document en format XML
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<front><div type="abstract" xml:lang="en"> <p>BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.</p>
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<author role="aut"> <persName> <forename type="first">Peter P</forename>
<surname>Cheung</surname>
</persName>
<idno type="halauthorid">603601</idno>
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<author role="aut"> <persName> <forename type="first">Y H Connie</forename>
<surname>Leung</surname>
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<idno type="halauthorid">603602</idno>
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<author role="aut"> <persName> <forename type="first">Chun-Kin</forename>
<surname>Chow</surname>
</persName>
<idno type="halauthorid">603603</idno>
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<author role="aut"> <persName> <forename type="first">Chi-Fung</forename>
<surname>Ng</surname>
</persName>
<idno type="halauthorid">603604</idno>
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<author role="aut"> <persName> <forename type="first">Chun-Lok</forename>
<surname>Tsang</surname>
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<author role="aut"> <persName> <forename type="first">Yu-On</forename>
<surname>Wu</surname>
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<idno type="halauthorid">603606</idno>
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<author role="aut"> <persName> <forename type="first">Siu-Kit</forename>
<surname>Ma</surname>
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<idno type="halauthorid">603607</idno>
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<author role="aut"> <persName> <forename type="first">Sin-Fun</forename>
<surname>Sia</surname>
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<author role="aut"> <persName> <forename type="first">Yi</forename>
<surname>Guan</surname>
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<idno type="halauthorid">603537</idno>
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<author role="crp"> <persName> <forename type="first">J. S. M.</forename>
<surname>Peiris</surname>
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<email type="md5">cc6ecab6abc0bb33758973d51e488f7b</email>
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<date type="whenReleased">2011-06-09 18:26:24</date>
<date type="whenProduced">2009-09</date>
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<idno type="halRefHtml">Journal of Clinical Virology, Elsevier, 2009, 46 (1), pp.90-3. ⟨10.1016/j.jcv.2009.06.016⟩</idno>
<idno type="halRef">Journal of Clinical Virology, Elsevier, 2009, 46 (1), pp.90-3. ⟨10.1016/j.jcv.2009.06.016⟩</idno>
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<idno type="stamp" n="RIIP_HONGKONG" corresp="RIIP">Centre de Recherche Université de Hong-Kong-Pasteur</idno>
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<sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.</title>
<author role="aut"> <persName> <forename type="first">Peter P</forename>
<surname>Cheung</surname>
</persName>
<idno type="halauthorid">603601</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Y H Connie</forename>
<surname>Leung</surname>
</persName>
<idno type="halauthorid">603602</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Chun-Kin</forename>
<surname>Chow</surname>
</persName>
<idno type="halauthorid">603603</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Chi-Fung</forename>
<surname>Ng</surname>
</persName>
<idno type="halauthorid">603604</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Chun-Lok</forename>
<surname>Tsang</surname>
</persName>
<idno type="halauthorid">603605</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Yu-On</forename>
<surname>Wu</surname>
</persName>
<idno type="halauthorid">603606</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Siu-Kit</forename>
<surname>Ma</surname>
</persName>
<idno type="halauthorid">603607</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Sin-Fun</forename>
<surname>Sia</surname>
</persName>
<idno type="halauthorid">603608</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Yi</forename>
<surname>Guan</surname>
</persName>
<idno type="halauthorid">603537</idno>
<affiliation ref="#struct-136023"></affiliation>
</author>
<author role="crp"> <persName> <forename type="first">J. S. M.</forename>
<surname>Peiris</surname>
</persName>
<email type="md5">cc6ecab6abc0bb33758973d51e488f7b</email>
<email type="domain">hkucc.hku.hk</email>
<idno type="halauthorid">603528</idno>
<affiliation ref="#struct-136023"></affiliation>
<affiliation ref="#struct-55925"></affiliation>
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</analytic>
<monogr> <idno type="halJournalId" status="VALID">15241</idno>
<idno type="issn">1386-6532</idno>
<title level="j">Journal of Clinical Virology</title>
<imprint> <publisher>Elsevier</publisher>
<biblScope unit="volume">46</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="pp">90-3</biblScope>
<date type="datePub">2009-09</date>
<date type="dateEpub">2009-07-14</date>
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</monogr>
<idno type="doi">10.1016/j.jcv.2009.06.016</idno>
<idno type="pubmed">19604718</idno>
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</sourceDesc>
<profileDesc> <langUsage> <language ident="en">English</language>
</langUsage>
<textClass> <classCode scheme="mesh">Animals</classCode>
<classCode scheme="mesh">Bird Diseases</classCode>
<classCode scheme="mesh">Birds</classCode>
<classCode scheme="mesh">DNA Primers</classCode>
<classCode scheme="mesh">Feces</classCode>
<classCode scheme="mesh">Influenza in Birds</classCode>
<classCode scheme="mesh">Reverse Transcriptase Polymerase Chain Reaction</classCode>
<classCode scheme="mesh">Sensitivity and Specificity</classCode>
<classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="en"> <p>BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.</p>
</abstract>
</profileDesc>
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