Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
Identifieur interne : 004175 ( PubMed/Corpus ); précédent : 004174; suivant : 004176Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
Auteurs : D. Beighton ; R. Ludford ; D T Clark ; S R Brailsford ; C L Pankhurst ; G F Tinsley ; J. Fiske ; D. Lewis ; B. Daly ; N. KhalifaSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 1995.
English descriptors
- KwdEn :
- MESH :
- chemical : Culture Media.
- isolation & purification : Candida, Saccharomyces cerevisiae.
- microbiology : Candidiasis, Oral, Dental Caries, Mouth Diseases, Mouth, Edentulous.
- Denture, Complete, Humans.
Abstract
A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.
PubMed: 8576366
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Ludford</name></author><author><name sortKey="Clark, D T" sort="Clark, D T" uniqKey="Clark D" first="D T" last="Clark">D T Clark</name></author><author><name sortKey="Brailsford, S R" sort="Brailsford, S R" uniqKey="Brailsford S" first="S R" last="Brailsford">S R Brailsford</name></author><author><name sortKey="Pankhurst, C L" sort="Pankhurst, C L" uniqKey="Pankhurst C" first="C L" last="Pankhurst">C L Pankhurst</name></author><author><name sortKey="Tinsley, G F" sort="Tinsley, G F" uniqKey="Tinsley G" first="G F" last="Tinsley">G F Tinsley</name></author><author><name sortKey="Fiske, J" sort="Fiske, J" uniqKey="Fiske J" first="J" last="Fiske">J. Fiske</name></author><author><name sortKey="Lewis, D" sort="Lewis, D" uniqKey="Lewis D" first="D" last="Lewis">D. Lewis</name></author><author><name sortKey="Daly, B" sort="Daly, B" uniqKey="Daly B" first="B" last="Daly">B. Daly</name></author><author><name sortKey="Khalifa, N" sort="Khalifa, N" uniqKey="Khalifa N" first="N" last="Khalifa">N. Khalifa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:8576366</idno><idno type="pmid">8576366</idno><idno type="wicri:Area/PubMed/Corpus">004175</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">004175</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Use of CHROMagar Candida medium for isolation of yeasts from dental samples.</title><author><name sortKey="Beighton, D" sort="Beighton, D" uniqKey="Beighton D" first="D" last="Beighton">D. Beighton</name><affiliation><nlm:affiliation>RCS Department of Dental Sciences, King's College School of Medicine and Dentistry, London, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Ludford, R" sort="Ludford, R" uniqKey="Ludford R" first="R" last="Ludford">R. Ludford</name></author><author><name sortKey="Clark, D T" sort="Clark, D T" uniqKey="Clark D" first="D T" last="Clark">D T Clark</name></author><author><name sortKey="Brailsford, S R" sort="Brailsford, S R" uniqKey="Brailsford S" first="S R" last="Brailsford">S R Brailsford</name></author><author><name sortKey="Pankhurst, C L" sort="Pankhurst, C L" uniqKey="Pankhurst C" first="C L" last="Pankhurst">C L Pankhurst</name></author><author><name sortKey="Tinsley, G F" sort="Tinsley, G F" uniqKey="Tinsley G" first="G F" last="Tinsley">G F Tinsley</name></author><author><name sortKey="Fiske, J" sort="Fiske, J" uniqKey="Fiske J" first="J" last="Fiske">J. Fiske</name></author><author><name sortKey="Lewis, D" sort="Lewis, D" uniqKey="Lewis D" first="D" last="Lewis">D. Lewis</name></author><author><name sortKey="Daly, B" sort="Daly, B" uniqKey="Daly B" first="B" last="Daly">B. Daly</name></author><author><name sortKey="Khalifa, N" sort="Khalifa, N" uniqKey="Khalifa N" first="N" last="Khalifa">N. Khalifa</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Candida (isolation & purification)</term><term>Candidiasis, Oral (microbiology)</term><term>Culture Media</term><term>Dental Caries (microbiology)</term><term>Denture, Complete</term><term>Humans</term><term>Mouth Diseases (microbiology)</term><term>Mouth, Edentulous (microbiology)</term><term>Saccharomyces cerevisiae (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Candida</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Candidiasis, Oral</term><term>Dental Caries</term><term>Mouth Diseases</term><term>Mouth, Edentulous</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Denture, Complete</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8576366</PMID><DateCompleted><Year>1996</Year><Month>03</Month><Day>12</Day></DateCompleted><DateRevised><Year>2013</Year><Month>09</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>11</Issue><PubDate><Year>1995</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Use of CHROMagar Candida medium for isolation of yeasts from dental samples.</ArticleTitle><Pagination><MedlinePgn>3025-7</MedlinePgn></Pagination><Abstract><AbstractText>A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.</AbstractText></Abstract><AuthorList CompleteYN="N"><Author ValidYN="Y"><LastName>Beighton</LastName><ForeName>D</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>RCS Department of Dental Sciences, King's College School of Medicine and Dentistry, London, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ludford</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Clark</LastName><ForeName>D T</ForeName><Initials>DT</Initials></Author><Author ValidYN="Y"><LastName>Brailsford</LastName><ForeName>S R</ForeName><Initials>SR</Initials></Author><Author ValidYN="Y"><LastName>Pankhurst</LastName><ForeName>C L</ForeName><Initials>CL</Initials></Author><Author ValidYN="Y"><LastName>Tinsley</LastName><ForeName>G F</ForeName><Initials>GF</Initials></Author><Author ValidYN="Y"><LastName>Fiske</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Lewis</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Daly</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Khalifa</LastName><ForeName>N</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Community Dent Oral Epidemiol. 1975 May;3(3):115-9</RefSource><PMID Version="1">1056815</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Dent Res. 1981 Dec;60(12):1929-35</RefSource><PMID Version="1">6946107</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Oral Pathol. 1986 Aug;15(7):386-8</RefSource><PMID Version="1">3098945</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 1987 Dec;25(12):2424-5</RefSource><PMID Version="1">3323233</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Oral Microbiol Immunol. 1990 Apr;5(2):82-5</RefSource><PMID Version="1">2087353</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Pathol Bacteriol. 1964 Apr;87:428-31</RefSource><PMID Version="1">14137706</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Spec Care Dentist. 1991 Nov-Dec;11(6):222-6</RefSource><PMID Version="1">1813993</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 1994 Aug;32(8):1923-9</RefSource><PMID Version="1">7989544</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 1994 Dec;32(12):3034-6</RefSource><PMID Version="1">7883894</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Antibiot Annu. 1957-1958;5:137-43</RefSource><PMID Version="1">13521798</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Community Dent Oral Epidemiol. 1991 Oct;19(5):302-7</RefSource><PMID Version="1">1742998</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D002175" MajorTopicYN="N">Candida</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002180" MajorTopicYN="N">Candidiasis, Oral</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="Y">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003731" MajorTopicYN="N">Dental Caries</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003824" MajorTopicYN="N">Denture, Complete</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009059" MajorTopicYN="N">Mouth Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009066" MajorTopicYN="N">Mouth, Edentulous</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC228627</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>11</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8576366</ArticleId><ArticleId IdType="pmc">PMC228627</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document 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