Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Identifieur interne : 000199 ( PubMed/Corpus ); précédent : 000198; suivant : 000200Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Auteurs : Allauddin Siddiqi ; Trudy Milne ; Mary P. Cullinan ; Gregory J. SeymourSource :
- Clinical oral implants research [ 1600-0501 ] ; 2016.
English descriptors
- KwdEn :
- DNA, Bacterial (analysis), Dental Implants, Gingiva (microbiology), Humans, Mouth, Edentulous (microbiology), Porphyromonas gingivalis (isolation & purification), Real-Time Polymerase Chain Reaction, Staphylococcus aureus (isolation & purification), Tannerella forsythia (isolation & purification), Titanium, Tongue (microbiology), Zirconium.
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical : Dental Implants, Titanium, Zirconium.
- isolation & purification : Porphyromonas gingivalis, Staphylococcus aureus, Tannerella forsythia.
- microbiology : Gingiva, Mouth, Edentulous, Tongue.
- Humans, Real-Time Polymerase Chain Reaction.
Abstract
It has been suggested that completely edentulous patients harbour fewer periodontopathic bacteria compared with dentate patients, due to the removal of the subgingival periodontal environment. However, reappearance of certain microbes has been reported after the placement of implants in these patients.
DOI: 10.1111/clr.12536
PubMed: 25529603
Links to Exploration step
pubmed:25529603Le document en format XML
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Cullinan</name><affiliation><nlm:affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</nlm:affiliation></affiliation></author><author><name sortKey="Seymour, Gregory J" sort="Seymour, Gregory J" uniqKey="Seymour G" first="Gregory J" last="Seymour">Gregory J. Seymour</name><affiliation><nlm:affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:25529603</idno><idno type="pmid">25529603</idno><idno type="doi">10.1111/clr.12536</idno><idno type="wicri:Area/PubMed/Corpus">000199</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000199</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.</title><author><name sortKey="Siddiqi, Allauddin" sort="Siddiqi, Allauddin" uniqKey="Siddiqi A" first="Allauddin" last="Siddiqi">Allauddin Siddiqi</name><affiliation><nlm:affiliation>Oral Implantology Research Group, Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</nlm:affiliation></affiliation></author><author><name sortKey="Milne, Trudy" sort="Milne, Trudy" uniqKey="Milne T" first="Trudy" last="Milne">Trudy Milne</name><affiliation><nlm:affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</nlm:affiliation></affiliation></author><author><name sortKey="Cullinan, Mary P" sort="Cullinan, Mary P" uniqKey="Cullinan M" first="Mary P" last="Cullinan">Mary P. Cullinan</name><affiliation><nlm:affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</nlm:affiliation></affiliation></author><author><name sortKey="Seymour, Gregory J" sort="Seymour, Gregory J" uniqKey="Seymour G" first="Gregory J" last="Seymour">Gregory J. Seymour</name><affiliation><nlm:affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Clinical oral implants research</title><idno type="eISSN">1600-0501</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Bacterial (analysis)</term><term>Dental Implants</term><term>Gingiva (microbiology)</term><term>Humans</term><term>Mouth, Edentulous (microbiology)</term><term>Porphyromonas gingivalis (isolation & purification)</term><term>Real-Time Polymerase Chain Reaction</term><term>Staphylococcus aureus (isolation & purification)</term><term>Tannerella forsythia (isolation & purification)</term><term>Titanium</term><term>Tongue (microbiology)</term><term>Zirconium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Dental Implants</term><term>Titanium</term><term>Zirconium</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Porphyromonas gingivalis</term><term>Staphylococcus aureus</term><term>Tannerella forsythia</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Gingiva</term><term>Mouth, Edentulous</term><term>Tongue</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Real-Time Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It has been suggested that completely edentulous patients harbour fewer periodontopathic bacteria compared with dentate patients, due to the removal of the subgingival periodontal environment. However, reappearance of certain microbes has been reported after the placement of implants in these patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25529603</PMID><DateCompleted><Year>2017</Year><Month>07</Month><Day>17</Day></DateCompleted><DateRevised><Year>2017</Year><Month>07</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1600-0501</ISSN><JournalIssue CitedMedium="Internet"><Volume>27</Volume><Issue>3</Issue><PubDate><Year>2016</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Clinical oral implants research</Title><ISOAbbreviation>Clin Oral Implants Res</ISOAbbreviation></Journal><ArticleTitle>Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.</ArticleTitle><Pagination><MedlinePgn>288-94</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/clr.12536</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">It has been suggested that completely edentulous patients harbour fewer periodontopathic bacteria compared with dentate patients, due to the removal of the subgingival periodontal environment. However, reappearance of certain microbes has been reported after the placement of implants in these patients.</AbstractText><AbstractText Label="AIM" NlmCategory="OBJECTIVE">The aim of this study was to determine whether the periodontopathic bacteria Porphyromonas gingivalis and Tannerella forsythia, as well as the non-periodontopathic bacterium, Staphylococcus aureus, emerged in edentulous patients 6 months after placement of one-piece zirconia and titanium implants.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">Twenty-six patients were included in the study (titanium = 13, zirconia = 13). Microbial samples were collected from the tongue prior to implant placement and 6 months after implant placement from both the tongue and from around the implants. A qRT-PCR assay using SYBR green/ROX chemistry was used for the detection and quantification of rgp, nuc and karilysin single-copy gene of P. gingivalis, T. forsythia and S. aureus, respectively. Positive controls used in the study were pure bacterial gDNA purified from cultures of P. gingivalis and S. aureus, a cloned sequence of the karilysin gene for T. forsythia, a plaque sample positive for P. gingivalis and T. forsythia, and nasal gDNA for S. aureus.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The results show that prior to implant placement, all three bacterial species were below the lower limit of quantification in all edentulous patients. The samples collected from the tongue and around the implants remained below the lower limit of quantification for each of the three species. However, all positive controls used in the study were detectable in the samples. qPCR standard curves showed correlation coefficients >0.97 and efficiencies >94.5% (slope range -3.19 to -3.46) for each of the SYBR green PCR assays.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The results of this study indicate that the tested organisms did not emerge 6 months after implant placement irrespective of the nature of the implant biomaterial. A further follow-up of at least 2 years post-implantation of these patients is suggested to determine whether there are any changes in the oral microbiota and whether such changes are associated with the development of peri-implant disease.</AbstractText><CopyrightInformation>© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Siddiqi</LastName><ForeName>Allauddin</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Oral Implantology Research Group, Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Milne</LastName><ForeName>Trudy</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cullinan</LastName><ForeName>Mary P</ForeName><Initials>MP</Initials><AffiliationInfo><Affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seymour</LastName><ForeName>Gregory J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Clin Oral Implants Res</MedlineTA><NlmUniqueID>9105713</NlmUniqueID><ISSNLinking>0905-7161</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015921">Dental Implants</NameOfSubstance></Chemical><Chemical><RegistryNumber>C6V6S92N3C</RegistryNumber><NameOfSubstance UI="D015040">Zirconium</NameOfSubstance></Chemical><Chemical><RegistryNumber>D1JT611TNE</RegistryNumber><NameOfSubstance UI="D014025">Titanium</NameOfSubstance></Chemical><Chemical><RegistryNumber>S38N85C5G0</RegistryNumber><NameOfSubstance UI="C028541">zirconium oxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015921" MajorTopicYN="N">Dental Implants</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005881" MajorTopicYN="N">Gingiva</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009066" MajorTopicYN="N">Mouth, Edentulous</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016966" MajorTopicYN="N">Porphyromonas gingivalis</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000070436" MajorTopicYN="N">Tannerella forsythia</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014025" MajorTopicYN="N">Titanium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014059" MajorTopicYN="N">Tongue</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015040" MajorTopicYN="N">Zirconium</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Porphyromonas gingivalis</Keyword><Keyword MajorTopicYN="N">Staphylococcus aureus</Keyword><Keyword MajorTopicYN="N">Tannerella forsythia</Keyword><Keyword MajorTopicYN="N">biofilm</Keyword><Keyword MajorTopicYN="N">edentulous patients</Keyword><Keyword MajorTopicYN="N">one-piece implant</Keyword><Keyword MajorTopicYN="N">qRT-PCR</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>11</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25529603</ArticleId><ArticleId IdType="doi">10.1111/clr.12536</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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