Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100A9), two calcium-binding proteins of the S100 family.
Identifieur interne : 002D21 ( PubMed/Checkpoint ); précédent : 002D20; suivant : 002D22Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100A9), two calcium-binding proteins of the S100 family.
Auteurs : T. Kojima [Suisse] ; E. Andersen ; J C Sanchez ; M R Wilkins ; D F Hochstrasser ; W F Pralong ; G. CimasoniSource :
- Journal of dental research [ 0022-0345 ] ; 2000.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Agents colorants, Analyse de séquence de protéine, Antigènes de différenciation (analyse), Antigènes de différenciation (sang), Argent, Bouche (métabolisme), Bouche édentée (métabolisme), Calgranuline A, Calgranuline B, Cartographie peptidique, Exsudat gingival (), Humains, Lasers, Marqueurs biologiques (analyse), Masse moléculaire, Nourrisson, Nouveau-né, Parodontite (métabolisme), Point isoélectrique, Protéines S100 (analyse), Protéines S100 (sang), Protéines de liaison au calcium (analyse), Protéines de liaison au calcium (sang), Salive (), Spectrométrie de masse, Sujet âgé, Technique de Western, Électrophorèse bidimensionnelle sur gel.
- MESH :
- analyse : Antigènes de différenciation, Marqueurs biologiques, Protéines S100, Protéines de liaison au calcium.
- métabolisme : Bouche, Bouche édentée, Parodontite.
- sang : Antigènes de différenciation, Protéines S100, Protéines de liaison au calcium.
- Adulte, Adulte d'âge moyen, Agents colorants, Analyse de séquence de protéine, Argent, Calgranuline A, Calgranuline B, Cartographie peptidique, Exsudat gingival, Humains, Lasers, Masse moléculaire, Nourrisson, Nouveau-né, Point isoélectrique, Salive, Spectrométrie de masse, Sujet âgé, Technique de Western, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Adult, Aged, Antigens, Differentiation (analysis), Antigens, Differentiation (blood), Biomarkers (analysis), Blotting, Western, Calcium-Binding Proteins (analysis), Calcium-Binding Proteins (blood), Calgranulin A, Calgranulin B, Coloring Agents, Electrophoresis, Gel, Two-Dimensional, Gingival Crevicular Fluid (chemistry), Humans, Infant, Infant, Newborn, Isoelectric Point, Lasers, Mass Spectrometry, Middle Aged, Molecular Weight, Mouth (metabolism), Mouth, Edentulous (metabolism), Peptide Mapping, Periodontitis (metabolism), S100 Proteins (analysis), S100 Proteins (blood), Saliva (chemistry), Sequence Analysis, Protein, Silver.
- MESH :
- chemical , analysis : Antigens, Differentiation, Biomarkers, Calcium-Binding Proteins, S100 Proteins.
- chemical , blood : Antigens, Differentiation, Calcium-Binding Proteins, S100 Proteins.
- chemistry : Gingival Crevicular Fluid, Saliva.
- metabolism : Mouth, Mouth, Edentulous, Periodontitis.
- Adult, Aged, Blotting, Western, Calgranulin A, Calgranulin B, Coloring Agents, Electrophoresis, Gel, Two-Dimensional, Humans, Infant, Infant, Newborn, Isoelectric Point, Lasers, Mass Spectrometry, Middle Aged, Molecular Weight, Peptide Mapping, Sequence Analysis, Protein, Silver.
Abstract
Human gingival crevicular fluid contains unidentified proteins which might play a role as markers in periodontal diseases. Therefore, low-molecular-weight proteins found in human gingival crevicular fluid (GCF), but absent from serum, were identified in the present study by means of two-dimensional electrophoresis (2-D PAGE) analysis. GCF, serum, and whole saliva were collected from periodontitis and healthy subjects, as well as from edentulous and newborn subjects. Protein samples were separated by two-dimensional polyacrylamide gel electrophoresis, stained with silver, and compared with reference protein maps in the SWISS-2D PAGE database. In GCF and saliva from periodontitis patients and healthy subjects, four dominant low-molecular-mass (from 8 to 14 kDa) acidic spots were observed. They were not found in serum and were less visible in saliva from edentulous and newborn subjects. From N-terminal amino acid sequencing, the two 2-D protein spots of 8 kDa and isoelectric points between 6.5 and 7.0 were both identified as protein MRP8 (SI00A8), a member of the S100 family of calcium-binding proteins. Using peptide mass fingerprinting and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), we identified the other two protein spots, with mass of 14 kDa and isoelectric points between 5.5 and 6.0, as protein MRP14 (S100A9), also belonging to the S100 family. The presence of MRP8 and MRP14 in GCF was confirmed by Western blot, with monoclonal antibodies. The two polypeptides, MRP8 and MRP14, identified in GCF represent the major difference between the 2-D PAGE patterns of serum and GCF, and we hypothesize that they may play an important role in the gingival sulcus and could represent possible markers for periodontal diseases.
DOI: 10.1177/00220345000790020701
PubMed: 10728975
Affiliations:
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Kojima</name><affiliation wicri:level="4"><nlm:affiliation>Division of Oral Physiopathology and Periodontology, School of Dentistry, Faculty of Medicine, University of Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Division of Oral Physiopathology and Periodontology, School of Dentistry, Faculty of Medicine, University of Geneva</wicri:regionArea><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName><orgName type="university">Université de Genève</orgName></affiliation></author><author><name sortKey="Andersen, E" sort="Andersen, E" uniqKey="Andersen E" first="E" last="Andersen">E. Andersen</name></author><author><name sortKey="Sanchez, J C" sort="Sanchez, J C" uniqKey="Sanchez J" first="J C" last="Sanchez">J C Sanchez</name></author><author><name sortKey="Wilkins, M R" sort="Wilkins, M R" uniqKey="Wilkins M" first="M R" last="Wilkins">M R Wilkins</name></author><author><name sortKey="Hochstrasser, D F" sort="Hochstrasser, D F" uniqKey="Hochstrasser D" first="D F" last="Hochstrasser">D F Hochstrasser</name></author><author><name sortKey="Pralong, W F" sort="Pralong, W F" uniqKey="Pralong W" first="W F" last="Pralong">W F Pralong</name></author><author><name sortKey="Cimasoni, G" sort="Cimasoni, G" uniqKey="Cimasoni G" first="G" last="Cimasoni">G. 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Therefore, low-molecular-weight proteins found in human gingival crevicular fluid (GCF), but absent from serum, were identified in the present study by means of two-dimensional electrophoresis (2-D PAGE) analysis. GCF, serum, and whole saliva were collected from periodontitis and healthy subjects, as well as from edentulous and newborn subjects. Protein samples were separated by two-dimensional polyacrylamide gel electrophoresis, stained with silver, and compared with reference protein maps in the SWISS-2D PAGE database. In GCF and saliva from periodontitis patients and healthy subjects, four dominant low-molecular-mass (from 8 to 14 kDa) acidic spots were observed. They were not found in serum and were less visible in saliva from edentulous and newborn subjects. From N-terminal amino acid sequencing, the two 2-D protein spots of 8 kDa and isoelectric points between 6.5 and 7.0 were both identified as protein MRP8 (SI00A8), a member of the S100 family of calcium-binding proteins. Using peptide mass fingerprinting and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), we identified the other two protein spots, with mass of 14 kDa and isoelectric points between 5.5 and 6.0, as protein MRP14 (S100A9), also belonging to the S100 family. The presence of MRP8 and MRP14 in GCF was confirmed by Western blot, with monoclonal antibodies. The two polypeptides, MRP8 and MRP14, identified in GCF represent the major difference between the 2-D PAGE patterns of serum and GCF, and we hypothesize that they may play an important role in the gingival sulcus and could represent possible markers for periodontal diseases.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10728975</PMID><DateCompleted><Year>2000</Year><Month>04</Month><Day>04</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-0345</ISSN><JournalIssue CitedMedium="Print"><Volume>79</Volume><Issue>2</Issue><PubDate><Year>2000</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of dental research</Title><ISOAbbreviation>J. Dent. Res.</ISOAbbreviation></Journal><ArticleTitle>Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100A9), two calcium-binding proteins of the S100 family.</ArticleTitle><Pagination><MedlinePgn>740-7</MedlinePgn></Pagination><Abstract><AbstractText>Human gingival crevicular fluid contains unidentified proteins which might play a role as markers in periodontal diseases. Therefore, low-molecular-weight proteins found in human gingival crevicular fluid (GCF), but absent from serum, were identified in the present study by means of two-dimensional electrophoresis (2-D PAGE) analysis. GCF, serum, and whole saliva were collected from periodontitis and healthy subjects, as well as from edentulous and newborn subjects. Protein samples were separated by two-dimensional polyacrylamide gel electrophoresis, stained with silver, and compared with reference protein maps in the SWISS-2D PAGE database. In GCF and saliva from periodontitis patients and healthy subjects, four dominant low-molecular-mass (from 8 to 14 kDa) acidic spots were observed. They were not found in serum and were less visible in saliva from edentulous and newborn subjects. From N-terminal amino acid sequencing, the two 2-D protein spots of 8 kDa and isoelectric points between 6.5 and 7.0 were both identified as protein MRP8 (SI00A8), a member of the S100 family of calcium-binding proteins. Using peptide mass fingerprinting and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), we identified the other two protein spots, with mass of 14 kDa and isoelectric points between 5.5 and 6.0, as protein MRP14 (S100A9), also belonging to the S100 family. The presence of MRP8 and MRP14 in GCF was confirmed by Western blot, with monoclonal antibodies. The two polypeptides, MRP8 and MRP14, identified in GCF represent the major difference between the 2-D PAGE patterns of serum and GCF, and we hypothesize that they may play an important role in the gingival sulcus and could represent possible markers for periodontal diseases.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kojima</LastName><ForeName>T</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Division of Oral Physiopathology and Periodontology, School of Dentistry, Faculty of Medicine, University of Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andersen</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Sanchez</LastName><ForeName>J C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Wilkins</LastName><ForeName>M R</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Hochstrasser</LastName><ForeName>D F</ForeName><Initials>DF</Initials></Author><Author ValidYN="Y"><LastName>Pralong</LastName><ForeName>W F</ForeName><Initials>WF</Initials></Author><Author ValidYN="Y"><LastName>Cimasoni</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Dent Res</MedlineTA><NlmUniqueID>0354343</NlmUniqueID><ISSNLinking>0022-0345</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000943">Antigens, Differentiation</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002135">Calcium-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D040501">Calgranulin A</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D040502">Calgranulin B</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004396">Coloring Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009418">S100 Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000943" MajorTopicYN="N">Antigens, Differentiation</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002135" MajorTopicYN="N">Calcium-Binding Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040501" MajorTopicYN="N">Calgranulin A</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040502" MajorTopicYN="N">Calgranulin B</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004396" MajorTopicYN="N">Coloring Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005883" MajorTopicYN="N">Gingival Crevicular Fluid</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007231" MajorTopicYN="N">Infant, Newborn</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007526" MajorTopicYN="N">Isoelectric Point</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007834" MajorTopicYN="N">Lasers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009055" MajorTopicYN="N">Mouth</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009066" MajorTopicYN="N">Mouth, Edentulous</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010449" MajorTopicYN="N">Peptide Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010518" MajorTopicYN="N">Periodontitis</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009418" MajorTopicYN="N">S100 Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012463" MajorTopicYN="N">Saliva</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020539" MajorTopicYN="N">Sequence Analysis, Protein</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>3</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>3</Month><Day>23</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>3</Month><Day>23</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10728975</ArticleId><ArticleId IdType="doi">10.1177/00220345000790020701</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Genève</li></region><settlement><li>Genève</li></settlement><orgName><li>Université de Genève</li></orgName></list><tree><noCountry><name sortKey="Andersen, E" sort="Andersen, E" uniqKey="Andersen E" first="E" last="Andersen">E. Andersen</name><name sortKey="Cimasoni, G" sort="Cimasoni, G" uniqKey="Cimasoni G" first="G" last="Cimasoni">G. Cimasoni</name><name sortKey="Hochstrasser, D F" sort="Hochstrasser, D F" uniqKey="Hochstrasser D" first="D F" last="Hochstrasser">D F Hochstrasser</name><name sortKey="Pralong, W F" sort="Pralong, W F" uniqKey="Pralong W" first="W F" last="Pralong">W F Pralong</name><name sortKey="Sanchez, J C" sort="Sanchez, J C" uniqKey="Sanchez J" first="J C" last="Sanchez">J C Sanchez</name><name sortKey="Wilkins, M R" sort="Wilkins, M R" uniqKey="Wilkins M" first="M R" last="Wilkins">M R Wilkins</name></noCountry><country name="Suisse"><region name="Canton de Genève"><name sortKey="Kojima, T" sort="Kojima, T" uniqKey="Kojima T" first="T" last="Kojima">T. Kojima</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|clé= pubmed:10728975
|texte= Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100A9), two calcium-binding proteins of the S100 family.
}}
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