Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants
Identifieur interne : 002148 ( Pmc/Corpus ); précédent : 002147; suivant : 002149Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants
Auteurs : Ps Kumar ; Mr Mason ; Mr Brooker ; K. O RienSource :
- Journal of Clinical Periodontology [ 0303-6979 ] ; 2012.
Abstract
Although it is established that peri-implantitis is a bacterially induced disease, little is known about the bacterial profile of peri-implant communities in health and disease. The purpose of the present investigation was to examine the microbial signatures of the peri-implant microbiome in health and disease.
Subgingival and submucosal plaque samples were collected from forty subjects with periodontitis, peri-implantitis, periodontal and peri-implant health and analyzed using 16S pyrosequencing.
Peri-implant biofilms demonstrated significantly lower diversity than subgingival biofilms in both health and disease, however, several species, including previously unsuspected and unknown organisms, were unique to this niche. The predominant species in peri-implant communities belonged to the genera
The peri-implant microbiome differs significantly from the periodontal community in both health and disease. Peri-implantitis is a microbially heterogeneous infection with predominantly gram-negative species, and is less complex than periodontitis.
Url:
DOI: 10.1111/j.1600-051X.2012.01856.x
PubMed: 22417294
PubMed Central: 3323747
Links to Exploration step
PMC:3323747Le document en format XML
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<author><name sortKey="Kumar, Ps" sort="Kumar, Ps" uniqKey="Kumar P" first="Ps" last="Kumar">Ps Kumar</name>
</author>
<author><name sortKey="Mason, Mr" sort="Mason, Mr" uniqKey="Mason M" first="Mr" last="Mason">Mr Mason</name>
</author>
<author><name sortKey="Brooker, Mr" sort="Brooker, Mr" uniqKey="Brooker M" first="Mr" last="Brooker">Mr Brooker</name>
</author>
<author><name sortKey="O Rien, K" sort="O Rien, K" uniqKey="O Rien K" first="K" last="O Rien">K. O Rien</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants</title>
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<author><name sortKey="Mason, Mr" sort="Mason, Mr" uniqKey="Mason M" first="Mr" last="Mason">Mr Mason</name>
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<author><name sortKey="Brooker, Mr" sort="Brooker, Mr" uniqKey="Brooker M" first="Mr" last="Brooker">Mr Brooker</name>
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<author><name sortKey="O Rien, K" sort="O Rien, K" uniqKey="O Rien K" first="K" last="O Rien">K. O Rien</name>
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<series><title level="j">Journal of Clinical Periodontology</title>
<idno type="ISSN">0303-6979</idno>
<idno type="eISSN">1600-051X</idno>
<imprint><date when="2012">2012</date>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Aim</title>
<p id="P1">Although it is established that peri-implantitis is a bacterially induced disease, little is known about the bacterial profile of peri-implant communities in health and disease. The purpose of the present investigation was to examine the microbial signatures of the peri-implant microbiome in health and disease.</p>
</sec>
<sec sec-type="materials|methods" id="S2"><title>Materials and methods</title>
<p id="P2">Subgingival and submucosal plaque samples were collected from forty subjects with periodontitis, peri-implantitis, periodontal and peri-implant health and analyzed using 16S pyrosequencing.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Peri-implant biofilms demonstrated significantly lower diversity than subgingival biofilms in both health and disease, however, several species, including previously unsuspected and unknown organisms, were unique to this niche. The predominant species in peri-implant communities belonged to the genera <italic>Butyrivibrio, Campylobacter, Eubacterium, Prevotella, Selenomonas</italic>
, <italic>Streptococcus</italic>
, <italic>Actinomyces, Leptotrichia, Propionibacterium, Peptococcus, Lactococcus</italic>
and <italic>Treponema.</italic>
Peri-implant disease was associated with lower levels of <italic>Prevotella</italic>
and <italic>Leptotrichia</italic>
and higher levels of <italic>Actinomyces, Peptococcus, Campylobacter,</italic>
non-mutans <italic>Streptococcus, Butyrivibrio,</italic>
and <italic>Streptococcus mutans</italic>
than healthy implants. These communities also demonstrated lower levels of <italic>Prevotella</italic>
, non-mutans <italic>Streptococcus, Lactobacillus, Selenomonas, Leptotrichia, Actinomyces</italic>
and higher levels of <italic>Peptococcus, Mycoplasma, Eubacterium, Campylobacter, Butyrivibrio, Streptococcus mutans,</italic>
and <italic>Treponema</italic>
when compared to periodontitis-associated biofilms.</p>
</sec>
<sec id="S4"><title>Conclusion</title>
<p id="P4">The peri-implant microbiome differs significantly from the periodontal community in both health and disease. Peri-implantitis is a microbially heterogeneous infection with predominantly gram-negative species, and is less complex than periodontitis.</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="en"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0425123</journal-id>
<journal-id journal-id-type="pubmed-jr-id">4645</journal-id>
<journal-id journal-id-type="nlm-ta">J Clin Periodontol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Clin. Periodontol.</journal-id>
<journal-title-group><journal-title>Journal of Clinical Periodontology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0303-6979</issn>
<issn pub-type="epub">1600-051X</issn>
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<article-meta><article-id pub-id-type="pmid">22417294</article-id>
<article-id pub-id-type="pmc">3323747</article-id>
<article-id pub-id-type="doi">10.1111/j.1600-051X.2012.01856.x</article-id>
<article-id pub-id-type="manuscript">NIHMS349691</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kumar</surname>
<given-names>PS</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Mason</surname>
<given-names>MR</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Brooker</surname>
<given-names>MR</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>O’Brien</surname>
<given-names>K</given-names>
</name>
</contrib>
<aff id="A1">Division of Periodontology, College of Dentistry, The Ohio State University, Columbus Ohio</aff>
</contrib-group>
<author-notes><corresp id="FN1"><label>*</label>
Corresponding author: Dr. Purnima Kumar, 4111 Postle Hall, 305, W 12<sup>th</sup>
Avenue, Columbus, Ohio 43210, Phone: 614 247 4532, Fax: 614 292 4612, <email>kumar.83@osu.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>17</day>
<month>1</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>14</day>
<month>3</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="ppub"><month>5</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>5</month>
<year>2013</year>
</pub-date>
<volume>39</volume>
<issue>5</issue>
<fpage>425</fpage>
<lpage>433</lpage>
<abstract><sec id="S1"><title>Aim</title>
<p id="P1">Although it is established that peri-implantitis is a bacterially induced disease, little is known about the bacterial profile of peri-implant communities in health and disease. The purpose of the present investigation was to examine the microbial signatures of the peri-implant microbiome in health and disease.</p>
</sec>
<sec sec-type="materials|methods" id="S2"><title>Materials and methods</title>
<p id="P2">Subgingival and submucosal plaque samples were collected from forty subjects with periodontitis, peri-implantitis, periodontal and peri-implant health and analyzed using 16S pyrosequencing.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Peri-implant biofilms demonstrated significantly lower diversity than subgingival biofilms in both health and disease, however, several species, including previously unsuspected and unknown organisms, were unique to this niche. The predominant species in peri-implant communities belonged to the genera <italic>Butyrivibrio, Campylobacter, Eubacterium, Prevotella, Selenomonas</italic>
, <italic>Streptococcus</italic>
, <italic>Actinomyces, Leptotrichia, Propionibacterium, Peptococcus, Lactococcus</italic>
and <italic>Treponema.</italic>
Peri-implant disease was associated with lower levels of <italic>Prevotella</italic>
and <italic>Leptotrichia</italic>
and higher levels of <italic>Actinomyces, Peptococcus, Campylobacter,</italic>
non-mutans <italic>Streptococcus, Butyrivibrio,</italic>
and <italic>Streptococcus mutans</italic>
than healthy implants. These communities also demonstrated lower levels of <italic>Prevotella</italic>
, non-mutans <italic>Streptococcus, Lactobacillus, Selenomonas, Leptotrichia, Actinomyces</italic>
and higher levels of <italic>Peptococcus, Mycoplasma, Eubacterium, Campylobacter, Butyrivibrio, Streptococcus mutans,</italic>
and <italic>Treponema</italic>
when compared to periodontitis-associated biofilms.</p>
</sec>
<sec id="S4"><title>Conclusion</title>
<p id="P4">The peri-implant microbiome differs significantly from the periodontal community in both health and disease. Peri-implantitis is a microbially heterogeneous infection with predominantly gram-negative species, and is less complex than periodontitis.</p>
</sec>
</abstract>
<kwd-group><kwd>Bacteria</kwd>
<kwd>dental implant</kwd>
<kwd>peri-implantitis</kwd>
<kwd>DNA</kwd>
<kwd>pyrosequencing</kwd>
<kwd>16S periodontitis</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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