Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study
Identifieur interne : 006D44 ( Ncbi/Curation ); précédent : 006D43; suivant : 006D45Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study
Auteurs : F. S. De Ponte ; G. Cutroneo ; R. Falzea ; G. Rizzo ; L. Catalfamo ; A. Favaloro ; G. Vermiglio ; M. Runci ; A. Centofanti ; G. AnastasiSource :
- European Journal of Histochemistry : EJH [ 1121-760X ] ; 2016.
Abstract
Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor. In conclusion, our findings show that the fresh frozen bone after six month of graft is for the most part viable bone, encouraging its use as an alternative to autogenous bone for reconstructing maxillary bone defects prior to implant.
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DOI: 10.4081/ejh.2016.2642
PubMed: 28076936
PubMed Central: 5178803
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S." last="De Ponte">F. S. De Ponte</name></author><author><name sortKey="Cutroneo, G" sort="Cutroneo, G" uniqKey="Cutroneo G" first="G." last="Cutroneo">G. Cutroneo</name></author><author><name sortKey="Falzea, R" sort="Falzea, R" uniqKey="Falzea R" first="R." last="Falzea">R. Falzea</name></author><author><name sortKey="Rizzo, G" sort="Rizzo, G" uniqKey="Rizzo G" first="G." last="Rizzo">G. Rizzo</name></author><author><name sortKey="Catalfamo, L" sort="Catalfamo, L" uniqKey="Catalfamo L" first="L." last="Catalfamo">L. Catalfamo</name></author><author><name sortKey="Favaloro, A" sort="Favaloro, A" uniqKey="Favaloro A" first="A." last="Favaloro">A. Favaloro</name></author><author><name sortKey="Vermiglio, G" sort="Vermiglio, G" uniqKey="Vermiglio G" first="G." last="Vermiglio">G. Vermiglio</name></author><author><name sortKey="Runci, M" sort="Runci, M" uniqKey="Runci M" first="M." last="Runci">M. Runci</name></author><author><name sortKey="Centofanti, A" sort="Centofanti, A" uniqKey="Centofanti A" first="A." last="Centofanti">A. Centofanti</name></author><author><name sortKey="Anastasi, G" sort="Anastasi, G" uniqKey="Anastasi G" first="G." last="Anastasi">G. Anastasi</name></author></analytic><series><title level="j">European Journal of Histochemistry : EJH</title><idno type="ISSN">1121-760X</idno><idno type="eISSN">2038-8306</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor. In conclusion, our findings show that the fresh frozen bone after six month of graft is for the most part viable bone, encouraging its use as an alternative to autogenous bone for reconstructing maxillary bone defects prior to implant.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Catone, Ga" uniqKey="Catone G">GA Catone</name></author><author><name sortKey="Reimer, Bl" uniqKey="Reimer B">BL Reimer</name></author><author><name sortKey="Mcneir, D" uniqKey="Mcneir D">D McNeir</name></author><author><name sortKey="Ray, R" uniqKey="Ray R">R. 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