Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study
Identifieur interne : 006D44 ( Ncbi/Merge ); précédent : 006D43; suivant : 006D45Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study
Auteurs : F. S. De Ponte ; G. Cutroneo ; R. Falzea ; G. Rizzo ; L. Catalfamo ; A. Favaloro ; G. Vermiglio ; M. Runci ; A. Centofanti ; G. AnastasiSource :
- European Journal of Histochemistry : EJH [ 1121-760X ] ; 2016.
Abstract
Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor. In conclusion, our findings show that the fresh frozen bone after six month of graft is for the most part viable bone, encouraging its use as an alternative to autogenous bone for reconstructing maxillary bone defects prior to implant.
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DOI: 10.4081/ejh.2016.2642
PubMed: 28076936
PubMed Central: 5178803
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study</title><author><name sortKey="De Ponte, F S" sort="De Ponte, F S" uniqKey="De Ponte F" first="F. S." last="De Ponte">F. S. De Ponte</name></author><author><name sortKey="Cutroneo, G" sort="Cutroneo, G" uniqKey="Cutroneo G" first="G." last="Cutroneo">G. Cutroneo</name></author><author><name sortKey="Falzea, R" sort="Falzea, R" uniqKey="Falzea R" first="R." last="Falzea">R. Falzea</name></author><author><name sortKey="Rizzo, G" sort="Rizzo, G" uniqKey="Rizzo G" first="G." last="Rizzo">G. Rizzo</name></author><author><name sortKey="Catalfamo, L" sort="Catalfamo, L" uniqKey="Catalfamo L" first="L." last="Catalfamo">L. Catalfamo</name></author><author><name sortKey="Favaloro, A" sort="Favaloro, A" uniqKey="Favaloro A" first="A." last="Favaloro">A. Favaloro</name></author><author><name sortKey="Vermiglio, G" sort="Vermiglio, G" uniqKey="Vermiglio G" first="G." last="Vermiglio">G. Vermiglio</name></author><author><name sortKey="Runci, M" sort="Runci, M" uniqKey="Runci M" first="M." last="Runci">M. Runci</name></author><author><name sortKey="Centofanti, A" sort="Centofanti, A" uniqKey="Centofanti A" first="A." last="Centofanti">A. Centofanti</name></author><author><name sortKey="Anastasi, G" sort="Anastasi, G" uniqKey="Anastasi G" first="G." last="Anastasi">G. Anastasi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28076936</idno><idno type="pmc">5178803</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5178803</idno><idno type="RBID">PMC:5178803</idno><idno type="doi">10.4081/ejh.2016.2642</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">001959</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001959</idno><idno type="wicri:Area/Pmc/Curation">001959</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001959</idno><idno type="wicri:Area/Pmc/Checkpoint">000669</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000669</idno><idno type="wicri:Area/Ncbi/Merge">006D44</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study</title><author><name sortKey="De Ponte, F S" sort="De Ponte, F S" uniqKey="De Ponte F" first="F. S." last="De Ponte">F. S. De Ponte</name></author><author><name sortKey="Cutroneo, G" sort="Cutroneo, G" uniqKey="Cutroneo G" first="G." last="Cutroneo">G. Cutroneo</name></author><author><name sortKey="Falzea, R" sort="Falzea, R" uniqKey="Falzea R" first="R." last="Falzea">R. Falzea</name></author><author><name sortKey="Rizzo, G" sort="Rizzo, G" uniqKey="Rizzo G" first="G." last="Rizzo">G. Rizzo</name></author><author><name sortKey="Catalfamo, L" sort="Catalfamo, L" uniqKey="Catalfamo L" first="L." last="Catalfamo">L. Catalfamo</name></author><author><name sortKey="Favaloro, A" sort="Favaloro, A" uniqKey="Favaloro A" first="A." last="Favaloro">A. Favaloro</name></author><author><name sortKey="Vermiglio, G" sort="Vermiglio, G" uniqKey="Vermiglio G" first="G." last="Vermiglio">G. Vermiglio</name></author><author><name sortKey="Runci, M" sort="Runci, M" uniqKey="Runci M" first="M." last="Runci">M. Runci</name></author><author><name sortKey="Centofanti, A" sort="Centofanti, A" uniqKey="Centofanti A" first="A." last="Centofanti">A. Centofanti</name></author><author><name sortKey="Anastasi, G" sort="Anastasi, G" uniqKey="Anastasi G" first="G." last="Anastasi">G. Anastasi</name></author></analytic><series><title level="j">European Journal of Histochemistry : EJH</title><idno type="ISSN">1121-760X</idno><idno type="eISSN">2038-8306</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor. In conclusion, our findings show that the fresh frozen bone after six month of graft is for the most part viable bone, encouraging its use as an alternative to autogenous bone for reconstructing maxillary bone defects prior to implant.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Catone, Ga" uniqKey="Catone G">GA Catone</name></author><author><name sortKey="Reimer, Bl" uniqKey="Reimer B">BL Reimer</name></author><author><name sortKey="Mcneir, D" uniqKey="Mcneir D">D McNeir</name></author><author><name sortKey="Ray, R" uniqKey="Ray R">R. Ray</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donovan, Mg" uniqKey="Donovan M">MG Donovan</name></author><author><name sortKey="Dickerson, Nc" uniqKey="Dickerson N">NC Dickerson</name></author><author><name sortKey="Mitchell, Jc" uniqKey="Mitchell J">JC Mitchell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Misch, Cm" uniqKey="Misch C">CM Misch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Capelli, M" uniqKey="Capelli M">M Capelli</name></author><author><name sortKey="Zuffetti, F" uniqKey="Zuffetti F">F Zuffetti</name></author><author><name sortKey="Del Fabbro, M" uniqKey="Del Fabbro M">M Del Fabbro</name></author><author><name sortKey="Testori, T" uniqKey="Testori T">T. Testori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nowzari, H" uniqKey="Nowzari H">H Nowzari</name></author><author><name sortKey="Aalam, Aa" uniqKey="Aalam A">AA Aalam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nkenke, E" uniqKey="Nkenke E">E Nkenke</name></author><author><name sortKey="Radespiel Troger, M" uniqKey="Radespiel Troger M">M Radespiel-Tröger</name></author><author><name sortKey="Wiltfang, J" uniqKey="Wiltfang J">J Wiltfang</name></author><author><name sortKey="Schultze Mosgau, S" uniqKey="Schultze Mosgau S">S Schultze-Mosgau</name></author><author><name sortKey="Winkler, G" uniqKey="Winkler G">G Winkler</name></author><author><name sortKey="Neukam, Fw" uniqKey="Neukam F">FW Neukam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cricchio, G1" uniqKey="Cricchio G">G1 Cricchio</name></author><author><name sortKey="Lundgren, S" uniqKey="Lundgren S">S. Lundgren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Acocella, A" uniqKey="Acocella A">A Acocella</name></author><author><name sortKey="Bertolai, R" uniqKey="Bertolai R">R Bertolai</name></author><author><name sortKey="Colafranceschi, M" uniqKey="Colafranceschi M">M Colafranceschi</name></author><author><name sortKey="Sacco, R" uniqKey="Sacco R">R. Sacco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gazdag, Ar" uniqKey="Gazdag A">AR Gazdag</name></author><author><name sortKey="Lane, Jm" uniqKey="Lane J">JM Lane</name></author><author><name sortKey="Glaser, D" uniqKey="Glaser D">D Glaser</name></author><author><name sortKey="Forster, Ra" uniqKey="Forster R">RA Forster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wong, K" uniqKey="Wong K">K Wong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dagtekin, G" uniqKey="Dagtekin G">G Dagtekin</name></author><author><name sortKey="Schiffer, R" uniqKey="Schiffer R">R Schiffer</name></author><author><name sortKey="Klein, B" uniqKey="Klein B">B Klein</name></author><author><name sortKey="Jahnen Dechent, W" uniqKey="Jahnen Dechent W">W Jahnen-Dechent</name></author><author><name sortKey="Zwadlo Klarwasser, G" uniqKey="Zwadlo Klarwasser G">G. Zwadlo-Klarwasser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Yj" uniqKey="Chen Y">YJ Chen</name></author><author><name sortKey="Wurtz, T" uniqKey="Wurtz T">T Wurtz</name></author><author><name sortKey="Wang, Cj" uniqKey="Wang C">CJ Wang</name></author><author><name sortKey="Kuo, Yr" uniqKey="Kuo Y">YR Kuo</name></author><author><name sortKey="Yang, Kd" uniqKey="Yang K">KD Yang</name></author><author><name sortKey="Huang, Hc" uniqKey="Huang H">HC Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Geiger, D" uniqKey="Geiger D">D Geiger</name></author><author><name sortKey="Debus, Es" uniqKey="Debus E">ES Debus</name></author><author><name sortKey="Ziegler, Ue" uniqKey="Ziegler U">UE Ziegler</name></author><author><name sortKey="Larena Avellaneda, A" uniqKey="Larena Avellaneda A">A Larena-Avellaneda</name></author><author><name sortKey="Frosch, M" uniqKey="Frosch M">M Frosch</name></author><author><name sortKey="Thiede, A" uniqKey="Thiede A">A Thiede</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lalani, Z" uniqKey="Lalani Z">Z Lalani</name></author><author><name sortKey="Wong, M" uniqKey="Wong M">M Wong</name></author><author><name sortKey="Brey, Em" uniqKey="Brey E">EM Brey</name></author><author><name sortKey="Mikos, Ag" uniqKey="Mikos A">AG Mikos</name></author><author><name sortKey="Duke, Pj" uniqKey="Duke P">PJ Duke</name></author><author><name sortKey="Miller, Mj" uniqKey="Miller M">MJ Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mori, S" uniqKey="Mori S">S Mori</name></author><author><name sortKey="Akagi, M" uniqKey="Akagi M">M Akagi</name></author><author><name sortKey="Kikuyama, A" uniqKey="Kikuyama A">A Kikuyama</name></author><author><name sortKey="Yasuda, Y" uniqKey="Yasuda Y">Y Yasuda</name></author><author><name sortKey="Hamanishi, C" uniqKey="Hamanishi C">C. Hamanishi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, H" uniqKey="Huang H">H Huang</name></author><author><name sortKey="Ma, L" uniqKey="Ma L">L Ma</name></author><author><name sortKey="Kyrkanides, S" uniqKey="Kyrkanides S">S. Kyrkanides</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spin Neto, R" uniqKey="Spin Neto R">R Spin-Neto</name></author><author><name sortKey="Landazuri Del Barrio, Ra" uniqKey="Landazuri Del Barrio R">RA Landazuri Del Barrio</name></author><author><name sortKey="Pereira, La" uniqKey="Pereira L">LA Pereira</name></author><author><name sortKey="Marcantonio, Ra" uniqKey="Marcantonio R">RA Marcantonio</name></author><author><name sortKey="Marcantonio, E" uniqKey="Marcantonio E">E Marcantonio</name></author><author><name sortKey="Marcantonio, E" uniqKey="Marcantonio E">E Marcantonio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ullmark, G" uniqKey="Ullmark G">G Ullmark</name></author><author><name sortKey="Obrant, Kj" uniqKey="Obrant K">KJ Obrant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anastasi, G" uniqKey="Anastasi G">G Anastasi</name></author><author><name sortKey="Cutroneo, G" uniqKey="Cutroneo G">G Cutroneo</name></author><author><name sortKey="Rizzo, G" uniqKey="Rizzo G">G Rizzo</name></author><author><name sortKey="Favaloro, A" uniqKey="Favaloro A">A. Favaloro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Di Mauro, D" uniqKey="Di Mauro D">D Di Mauro</name></author><author><name sortKey="Gaeta, R" uniqKey="Gaeta R">R Gaeta</name></author><author><name sortKey="Arco, A" uniqKey="Arco A">A Arco</name></author><author><name sortKey="Milardi, D" uniqKey="Milardi D">D Milardi</name></author><author><name sortKey="Lentini, S" uniqKey="Lentini S">S Lentini</name></author><author><name sortKey="Runci, M" uniqKey="Runci M">M Runci</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kahnberg, Ke" uniqKey="Kahnberg K">KE Kahnberg</name></author><author><name sortKey="Nystrom, E" uniqKey="Nystrom E">E Nystrom</name></author><author><name sortKey="Bartholdsson, L" uniqKey="Bartholdsson L">L. Bartholdsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jensen, J" uniqKey="Jensen J">J Jensen</name></author><author><name sortKey="Sindet Pedersen, S" uniqKey="Sindet Pedersen S">S Sindet-Pedersen</name></author><author><name sortKey="Oliver, Aj" uniqKey="Oliver A">AJ Oliver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Acocella, A" uniqKey="Acocella A">A Acocella</name></author><author><name sortKey="Bertolai, R" uniqKey="Bertolai R">R Bertolai</name></author><author><name sortKey="Nissan, J" uniqKey="Nissan J">J Nissan</name></author><author><name sortKey="Sacco, R" uniqKey="Sacco R">R. Sacco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spin Neto, R" uniqKey="Spin Neto R">R Spin-Neto</name></author><author><name sortKey="Stavropoulos, A" uniqKey="Stavropoulos A">A Stavropoulos</name></author><author><name sortKey="Coletti, Fl" uniqKey="Coletti F">FL Coletti</name></author><author><name sortKey="Pereira, La" uniqKey="Pereira L">LA Pereira</name></author><author><name sortKey="Marcantonio, E" uniqKey="Marcantonio E">E Marcantonio</name></author><author><name sortKey="Wenzel, A" uniqKey="Wenzel A">A. Wenzel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Proussaefs, P" uniqKey="Proussaefs P">P Proussaefs</name></author><author><name sortKey="Lozada, J" uniqKey="Lozada J">J. Lozada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holmquist, P" uniqKey="Holmquist P">P Holmquist</name></author><author><name sortKey="Dasmah, A" uniqKey="Dasmah A">A Dasmah</name></author><author><name sortKey="Sennerby, L" uniqKey="Sennerby L">L Sennerby</name></author><author><name sortKey="Hallman, M" uniqKey="Hallman M">M. Hallman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Contar, Cm" uniqKey="Contar C">CM Contar</name></author><author><name sortKey="Sarot, Jr" uniqKey="Sarot J">JR Sarot</name></author><author><name sortKey="Bordini, J" uniqKey="Bordini J">J Bordini</name></author><author><name sortKey="Galvao, Gh" uniqKey="Galvao G">GH Galvão</name></author><author><name sortKey="Nicolau, Gv" uniqKey="Nicolau G">GV Nicolau</name></author><author><name sortKey="Machado, Ma" uniqKey="Machado M">MA Machado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morelli, T" uniqKey="Morelli T">T Morelli</name></author><author><name sortKey="Neiva, R" uniqKey="Neiva R">R Neiva</name></author><author><name sortKey="Wang, Hl" uniqKey="Wang H">HL Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waasdorp, J" uniqKey="Waasdorp J">J Waasdorp</name></author><author><name sortKey="Reynolds, Ma" uniqKey="Reynolds M">MA Reynolds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nissan, J" uniqKey="Nissan J">J Nissan</name></author><author><name sortKey="Mardinger, O" uniqKey="Mardinger O">O Mardinger</name></author><author><name sortKey="Calderon, S" uniqKey="Calderon S">S Calderon</name></author><author><name sortKey="Romanos, Ge" uniqKey="Romanos G">GE Romanos</name></author><author><name sortKey="Chaushu, G" uniqKey="Chaushu G">G. Chaushu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nissan, J" uniqKey="Nissan J">J Nissan</name></author><author><name sortKey="Ghelfan, O" uniqKey="Ghelfan O">O Ghelfan</name></author><author><name sortKey="Mardinger, O" uniqKey="Mardinger O">O Mardinger</name></author><author><name sortKey="Calderon, S" uniqKey="Calderon S">S Calderon</name></author><author><name sortKey="Chaushu, G" uniqKey="Chaushu G">G. Chaushu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Eur J Histochem</journal-id><journal-id journal-id-type="iso-abbrev">Eur J Histochem</journal-id><journal-id journal-id-type="publisher-id">EJH</journal-id><journal-title-group><journal-title>European Journal of Histochemistry : EJH</journal-title></journal-title-group><issn pub-type="ppub">1121-760X</issn><issn pub-type="epub">2038-8306</issn><publisher><publisher-name>PAGEPress Publications, Pavia, Italy</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28076936</article-id><article-id pub-id-type="pmc">5178803</article-id><article-id pub-id-type="doi">10.4081/ejh.2016.2642</article-id><article-categories><subj-group subj-group-type="heading"><subject>Brief Report</subject></subj-group></article-categories><title-group><article-title>Histochemical and Morphological Aspects of Fresh Frozen Bone: A Preliminary Study</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>De Ponte</surname><given-names>F.S.</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Cutroneo</surname><given-names>G.</given-names></name><xref ref-type="corresp" rid="cor1"></xref></contrib><contrib contrib-type="author"><name><surname>Falzea</surname><given-names>R.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Rizzo</surname><given-names>G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Catalfamo</surname><given-names>L.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Favaloro</surname><given-names>A.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Vermiglio</surname><given-names>G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Runci</surname><given-names>M.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Centofanti</surname><given-names>A.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Anastasi</surname><given-names>G.</given-names></name></contrib></contrib-group><aff>Department of Biomedical and Dental Sciences and Morphofunctional Imaging,<institution>University of Messina</institution>,<addr-line>Italy</addr-line></aff><author-notes><corresp id="cor1">Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, Università di Messina, Policlinico Universitario, Torre Biologica, Via Consolare Valeria 1, 98125 Messina, Italy. <phone>+39.090.2213361</phone> - <fax>+39.090.692449</fax>. <email>gcutroneo@unime.it</email></corresp><fn fn-type="con"><p>Contributions: GC, FSD, GA, RF, LC, study design; GV, AC, AF, GR, experimental procedures, immunohistochemical processing; MR, LC, RF, collection of biopsies; AF, GV, GR, AC, GC, microscopic analysis, data collection and/or preparation of figures. All authors have reviewed and contributed to the different draft versions of the manuscript and have read and approved the final manuscript.</p></fn><fn fn-type="COI-statement"><p>Conflict of interest: the authors declare no conflict of interest.</p></fn></author-notes><pub-date pub-type="epub"><day>06</day><month>12</month><year>2016</year></pub-date><pub-date pub-type="collection"><day>02</day><month>11</month><year>2016</year></pub-date><volume>60</volume><issue>4</issue><elocation-id>2642</elocation-id><history><date date-type="received"><day>16</day><month>3</month><year>2016</year></date><date date-type="accepted"><day>17</day><month>11</month><year>2016</year></date></history><permissions><copyright-statement>©Copyright F.S. De Ponte et al.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Licensee PAGEPress, Italy</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<uri xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</uri>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor. In conclusion, our findings show that the fresh frozen bone after six month of graft is for the most part viable bone, encouraging its use as an alternative to autogenous bone for reconstructing maxillary bone defects prior to implant.</p></abstract><kwd-group><title>Key words</title><kwd>Fresh frozen bone</kwd><kwd>osteoclast</kwd><kwd>osteoblast</kwd><kwd>VEGF</kwd><kwd>fluorescence antibody technique</kwd><kwd>histological techniques</kwd></kwd-group><counts><fig-count count="3"></fig-count><table-count count="0"></table-count><equation-count count="0"></equation-count><ref-count count="31"></ref-count><page-count count="5"></page-count></counts></article-meta></front><floats-group><fig id="fig001" orientation="portrait" position="float"><label>Figure 1.</label><caption><p>Compound panel of FFB grade 0 images obtained by hematoxylin-eosin, scanning electron microscopy and immunofluorescence techniques. A) Hematoxylin-eosin images show bone tissue characterized by empty osteocyte lacunae and also the presence of empty Volkmann’s (head arrow) and Haversian canals (arrow) (B). C) Scanning electron microscopy image shows FFB grade 0 characterized by empty osteocyte lacunae (red arrow) and absence of cellular components. Immunofluorescence reactions images show the absence of fluorescence for RANKR (D) and osteocalcin (E); in F) it is also possible to observe the presence of VEGF fluorescence pattern within some osteocyte lacunae (asterix).</p></caption><graphic xlink:href="ejh-2016-4-2642-g001"></graphic></fig><fig id="fig002" orientation="portrait" position="float"><label>Figure 2.</label><caption><p>Compound panel of FFB after six month of maxillary graft obtained by hematoxylin-eosin and SEM techniques. A) Image shows the presence of two areas: <italic>a</italic> and <italic>b</italic> areas; a areas is characterized by full osteocyte lacunae (magnification in the top right corner). B) Well formed bone marrow in the b area. C) <italic>a</italic> area with bone lamellae parallel to each other and partially overlapping like roof tiles, alternating to bone lamellae with the same architecture, but with opposite orientation (asterisk) and a well formed bone marrow with adipocyte (arrow) and endothelial cells (head arrow). D) <italic>b</italic> areas characterized by empty osteocyte lacunae surrounding the bone island (arrow) with full osteocyte lacunae. SEM images show the presence of full (head arrow) and empty (arrow) osteocyte lacunae (E) and Volkmann's canal (F, asterisk). E) magnification: 1500x.</p></caption><graphic xlink:href="ejh-2016-4-2642-g002"></graphic></fig><fig id="fig003" orientation="portrait" position="float"><label>Figure 3.</label><caption><p>Compound panel of five images of immunofluorescence reactions performed on FFB after six month of graft. A) It is possible to observe the presence of RANKR positive cells (red channel), which are osteoclast located also within Howship’s lacunae (head arrow) (B). C), D) Presence of osteocalcin positive cells (green channel), which correspond to osteoblasts, at mineralization side (head arrow). E) It is possible to observe VEGF fluorescence pattern (red channel) within osteocytic lacunae.</p></caption><graphic xlink:href="ejh-2016-4-2642-g003"></graphic></fig></floats-group></pmc><affiliations><list></list><tree><noCountry><name sortKey="Anastasi, G" sort="Anastasi, G" uniqKey="Anastasi G" first="G." last="Anastasi">G. Anastasi</name><name sortKey="Catalfamo, L" sort="Catalfamo, L" uniqKey="Catalfamo L" first="L." last="Catalfamo">L. Catalfamo</name><name sortKey="Centofanti, A" sort="Centofanti, A" uniqKey="Centofanti A" first="A." last="Centofanti">A. Centofanti</name><name sortKey="Cutroneo, G" sort="Cutroneo, G" uniqKey="Cutroneo G" first="G." last="Cutroneo">G. Cutroneo</name><name sortKey="De Ponte, F S" sort="De Ponte, F S" uniqKey="De Ponte F" first="F. S." last="De Ponte">F. S. De Ponte</name><name sortKey="Falzea, R" sort="Falzea, R" uniqKey="Falzea R" first="R." last="Falzea">R. Falzea</name><name sortKey="Favaloro, A" sort="Favaloro, A" uniqKey="Favaloro A" first="A." last="Favaloro">A. Favaloro</name><name sortKey="Rizzo, G" sort="Rizzo, G" uniqKey="Rizzo G" first="G." last="Rizzo">G. Rizzo</name><name sortKey="Runci, M" sort="Runci, M" uniqKey="Runci M" first="M." last="Runci">M. Runci</name><name sortKey="Vermiglio, G" sort="Vermiglio, G" uniqKey="Vermiglio G" first="G." last="Vermiglio">G. Vermiglio</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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