Cranial bone regeneration according to different particle sizes and densities of demineralized dentin matrix in the rabbit model
Identifieur interne : 000930 ( Main/Exploration ); précédent : 000929; suivant : 000931Cranial bone regeneration according to different particle sizes and densities of demineralized dentin matrix in the rabbit model
Auteurs : Jin-Woo Nam ; Moon-Young Kim ; Se-Jin HanSource :
- Maxillofacial Plastic and Reconstructive Surgery [ 2288-8101 ] ; 2016.
Abstract
The objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM).
We selected nine healthy male rabbits that were raised under the same conditions and that weighed about 3 kg. Two circular defects 8 mm in diameter were created in each side of the cranium. The defects were grafted with DDM using four different particle sizes and densities: 0.1 mL of 0.25- to 1.0-mm particles (group 1); 0.2 mL of 0.25- to 1.0-mm particles (group 2); 0.1 mL of 1.0- to 2.0-mm particles (group 3); and 0.2 mL of 1.0- to 2.0-mm particles (group 4). After 2, 4, and 8 weeks, the rabbits were sacrificed, and bone samples were evaluated by means of histologic, histomorphometric, and quantitative RT-PCR analysis.
In group 1, osteoblast activity and bone formation were greater than in the other three groups on histological examination. In groups 2, 3, and 4, dense connective tissue was seen around original bone even after 8 weeks. Histomorphometric analysis of representative sections in group 1 showed a higher rate of new bone formation, but the difference from the other groups was not statistically significant. RT-PCR analysis indicated a correlation between bone formation and protein (osteonectin and osteopontin) expression.
DDM with a space between particles of 200 μm was effective in bone formation, suggesting that materials with a small particle size could reasonably be used for bone grafting.
Url:
DOI: 10.1186/s40902-016-0073-1
PubMed: 27441186
PubMed Central: 4932123
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cranial bone regeneration according to different particle sizes and densities of demineralized dentin matrix in the rabbit model</title><author><name sortKey="Nam, Jin Woo" sort="Nam, Jin Woo" uniqKey="Nam J" first="Jin-Woo" last="Nam">Jin-Woo Nam</name><affiliation><nlm:aff id="Aff1">Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam South Korea</nlm:aff><wicri:noCountry code="subfield">Chungnam South Korea</wicri:noCountry></affiliation></author><author><name sortKey="Kim, Moon Young" sort="Kim, Moon Young" uniqKey="Kim M" first="Moon-Young" last="Kim">Moon-Young Kim</name><affiliation><nlm:aff id="Aff1">Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam South Korea</nlm:aff><wicri:noCountry code="subfield">Chungnam South Korea</wicri:noCountry></affiliation></author><author><name sortKey="Han, Se Jin" sort="Han, Se Jin" uniqKey="Han S" first="Se-Jin" last="Han">Se-Jin Han</name><affiliation><nlm:aff id="Aff1">Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam South Korea</nlm:aff><wicri:noCountry code="subfield">Chungnam South Korea</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27441186</idno><idno type="pmc">4932123</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932123</idno><idno type="RBID">PMC:4932123</idno><idno type="doi">10.1186/s40902-016-0073-1</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000D33</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D33</idno><idno type="wicri:Area/Pmc/Curation">000D33</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D33</idno><idno type="wicri:Area/Pmc/Checkpoint">000784</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000784</idno><idno type="wicri:Area/Ncbi/Merge">006902</idno><idno type="wicri:Area/Ncbi/Curation">006902</idno><idno type="wicri:Area/Ncbi/Checkpoint">006902</idno><idno type="wicri:doubleKey">2288-8101:2016:Nam J:cranial:bone:regeneration</idno><idno type="wicri:Area/Main/Merge">000930</idno><idno type="wicri:Area/Main/Curation">000930</idno><idno type="wicri:Area/Main/Exploration">000930</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cranial bone regeneration according to different particle sizes and densities of demineralized dentin matrix in the rabbit model</title><author><name sortKey="Nam, Jin Woo" sort="Nam, Jin Woo" uniqKey="Nam J" first="Jin-Woo" last="Nam">Jin-Woo Nam</name><affiliation><nlm:aff id="Aff1">Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam South Korea</nlm:aff><wicri:noCountry code="subfield">Chungnam South Korea</wicri:noCountry></affiliation></author><author><name sortKey="Kim, Moon Young" sort="Kim, Moon Young" uniqKey="Kim M" first="Moon-Young" last="Kim">Moon-Young Kim</name><affiliation><nlm:aff id="Aff1">Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam South Korea</nlm:aff><wicri:noCountry code="subfield">Chungnam South Korea</wicri:noCountry></affiliation></author><author><name sortKey="Han, Se Jin" sort="Han, Se Jin" uniqKey="Han S" first="Se-Jin" last="Han">Se-Jin Han</name><affiliation><nlm:aff id="Aff1">Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam South Korea</nlm:aff><wicri:noCountry code="subfield">Chungnam South Korea</wicri:noCountry></affiliation></author></analytic><series><title level="j">Maxillofacial Plastic and Reconstructive Surgery</title><idno type="ISSN">2288-8101</idno><idno type="eISSN">2288-8586</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM).</p></sec><sec><title>Methods</title><p>We selected nine healthy male rabbits that were raised under the same conditions and that weighed about 3 kg. Two circular defects 8 mm in diameter were created in each side of the cranium. The defects were grafted with DDM using four different particle sizes and densities: 0.1 mL of 0.25- to 1.0-mm particles (group 1); 0.2 mL of 0.25- to 1.0-mm particles (group 2); 0.1 mL of 1.0- to 2.0-mm particles (group 3); and 0.2 mL of 1.0- to 2.0-mm particles (group 4). After 2, 4, and 8 weeks, the rabbits were sacrificed, and bone samples were evaluated by means of histologic, histomorphometric, and quantitative RT-PCR analysis.</p></sec><sec><title>Results</title><p>In group 1, osteoblast activity and bone formation were greater than in the other three groups on histological examination. In groups 2, 3, and 4, dense connective tissue was seen around original bone even after 8 weeks. Histomorphometric analysis of representative sections in group 1 showed a higher rate of new bone formation, but the difference from the other groups was not statistically significant. RT-PCR analysis indicated a correlation between bone formation and protein (osteonectin and osteopontin) expression.</p></sec><sec><title>Conclusions</title><p>DDM with a space between particles of 200 μm was effective in bone formation, suggesting that materials with a small particle size could reasonably be used for bone grafting.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Boyne, Pj" uniqKey="Boyne P">PJ Boyne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Masters, Dh" uniqKey="Masters D">DH Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jarcho, M" uniqKey="Jarcho M">M Jarcho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arrington, Ed" uniqKey="Arrington E">ED Arrington</name></author><author><name sortKey="Smith, Wj" uniqKey="Smith W">WJ Smith</name></author><author><name sortKey="Chambers, Hg" 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sortKey="Han, Se Jin" sort="Han, Se Jin" uniqKey="Han S" first="Se-Jin" last="Han">Se-Jin Han</name><name sortKey="Kim, Moon Young" sort="Kim, Moon Young" uniqKey="Kim M" first="Moon-Young" last="Kim">Moon-Young Kim</name><name sortKey="Nam, Jin Woo" sort="Nam, Jin Woo" uniqKey="Nam J" first="Jin-Woo" last="Nam">Jin-Woo Nam</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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