Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation
Identifieur interne : 007A53 ( Main/Curation ); précédent : 007A52; suivant : 007A54Cytoskeletal organization of human mesenchymal stem cells (MSC) changes during their osteogenic differentiation
Auteurs : J. Pablo Rodríguez [Chili] ; Mauricio González [Chili] ; Susana Ríos [Chili] ; Ver Nica Cambiazo [Chili]Source :
- Journal of Cellular Biochemistry [ 0730-2312 ] ; 2004-11-01.
Descripteurs français
- Wicri :
- geographic : Chili.
English descriptors
- KwdEn :
- Actin, Actin cytoskeleton, Actin organization, Alexa fluor, Alkaline, Alkaline phosphatase activity, Basal, Basal conditions, Biochem, Biochemical markers, Bone marrow, Calcium deposition, Calcium phosphate deposition, Cell biochem, Cell biochem koukouritaki, Cell layer, Cell morphology, Cell shape, Characteristic phenotype, Chile, Cortical organization, Culture medium, Cytochalasin, Cytoskeletal, Cytoskeletal integrity, Cytoskeletal organization, Cytoskeleton, Cytoskeleton organization, Different donors, Differentiation, Differentiation markers, Entire cytoplasm, Human mesenchymal, Human mscs, Koukouritaki, Mesenchymal, Microtubule, Microtubule network, Morphological changes, Morphology, Msc, Nocodazole, Normal bone, Osteoblast, Osteoblastic, Osteogenic, Osteogenic conditions, Osteogenic differentiation, Osteogenic medium, Osteoporotic, Phenotype, Phosphatase, Rapid changes, Results show, Tubulin, Untreated, Untreated cells, Upper panels.
- Teeft :
- Actin, Actin cytoskeleton, Actin organization, Alexa fluor, Alkaline, Alkaline phosphatase activity, Basal, Basal conditions, Biochem, Biochemical markers, Bone marrow, Calcium deposition, Calcium phosphate deposition, Cell biochem, Cell biochem koukouritaki, Cell layer, Cell morphology, Cell shape, Characteristic phenotype, Chile, Cortical organization, Culture medium, Cytochalasin, Cytoskeletal, Cytoskeletal integrity, Cytoskeletal organization, Cytoskeleton, Cytoskeleton organization, Different donors, Differentiation, Differentiation markers, Entire cytoplasm, Human mesenchymal, Human mscs, Koukouritaki, Mesenchymal, Microtubule, Microtubule network, Morphological changes, Morphology, Msc, Nocodazole, Normal bone, Osteoblast, Osteoblastic, Osteogenic, Osteogenic conditions, Osteogenic differentiation, Osteogenic medium, Osteoporotic, Phenotype, Phosphatase, Rapid changes, Results show, Tubulin, Untreated, Untreated cells, Upper panels.
Abstract
Human MSCs have been studied to define the mechanisms involved in normal bone remodeling and the regulation of osteogenesis. During osteogenic differentiation, MSCs change from their characteristic fibroblast‐like phenotype to near spherical shape. In this study, we analyzed the correlation between the organization of cytoskeleton of MSCs, changes in cell morphology, and the expression of specific markers (alkaline phosphatase activity and calcium deposition) of osteogenic differentiation. For osteoblastic differentiation, cells were cultured in a culture medium supplemented with 100 nM dexamethasone, 10 mM β‐ glycerophosphate, and 50 μg/ml ascorbic acid. The organization of microfilaments and microtubules was examined by inmunofluorescence using Alexa fluor 594 phalloidin and anti α‐tubulin monoclonal antibody. Cytochalasin D and nocodazole were used to alter reversibly the cytoskeleton dynamic. A remarkable change in cytoskeleton organization was observed in human MSCs during osteogenic differentiation. Actin cytoskeleton changed from a large number of thin, parallel microfilament bundles extending across the entire cytoplasm in undifferentiated MSCs to a few thick actin filament bundles located at the outermost periphery in differentiated cells. Under osteogenic culture conditions, a reversible reorganization of microfilaments induced by an initial treatment with cytochalasin D but not with nocodazole reduced the expression of differentiation markers, without affecting the final morphology of the cells. The results indicate that changes in the assembly and disassembly kinetics of microfilaments dynamic of actin network formation may be critical in supporting the osteogenic differentiation of human MSCs; also indicated that the organization of microtubules appears to have a regulatory role on the kinetic of this process. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jcb.20234
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<front><div type="abstract" xml:lang="en">Human MSCs have been studied to define the mechanisms involved in normal bone remodeling and the regulation of osteogenesis. During osteogenic differentiation, MSCs change from their characteristic fibroblast‐like phenotype to near spherical shape. In this study, we analyzed the correlation between the organization of cytoskeleton of MSCs, changes in cell morphology, and the expression of specific markers (alkaline phosphatase activity and calcium deposition) of osteogenic differentiation. For osteoblastic differentiation, cells were cultured in a culture medium supplemented with 100 nM dexamethasone, 10 mM β‐ glycerophosphate, and 50 μg/ml ascorbic acid. The organization of microfilaments and microtubules was examined by inmunofluorescence using Alexa fluor 594 phalloidin and anti α‐tubulin monoclonal antibody. Cytochalasin D and nocodazole were used to alter reversibly the cytoskeleton dynamic. A remarkable change in cytoskeleton organization was observed in human MSCs during osteogenic differentiation. Actin cytoskeleton changed from a large number of thin, parallel microfilament bundles extending across the entire cytoplasm in undifferentiated MSCs to a few thick actin filament bundles located at the outermost periphery in differentiated cells. Under osteogenic culture conditions, a reversible reorganization of microfilaments induced by an initial treatment with cytochalasin D but not with nocodazole reduced the expression of differentiation markers, without affecting the final morphology of the cells. The results indicate that changes in the assembly and disassembly kinetics of microfilaments dynamic of actin network formation may be critical in supporting the osteogenic differentiation of human MSCs; also indicated that the organization of microtubules appears to have a regulatory role on the kinetic of this process. © 2004 Wiley‐Liss, Inc.</div>
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