Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study
Identifieur interne : 000A42 ( Istex/Curation ); précédent : 000A41; suivant : 000A43Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study
Auteurs : Fan Yang [République populaire de Chine] ; Rong Zhang [République populaire de Chine] ; Fuming He [République populaire de Chine] ; Xiao-Xiang Wang [République populaire de Chine] ; Shifang Zhao [République populaire de Chine] ; Guoli Yang [République populaire de Chine]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2012-06.
Descripteurs français
- Wicri :
- topic : Titane.
English descriptors
- KwdEn :
- Assay, Assay solution, Biomed mater, Biomedical materials research, Biomimetic, Biomimetic calcium phosphate coating, Biomimetic coatings, Bone formation, Bone loss, Cell attachment, Cell culture medium sample, Cell lysate, Cellular protein, Cellytic buffer, Chemical compositions, Collagen, Collagen synthesis, Collagen synthesis assay, Collagen type, Contract grant number, Contract grant sponsor, Control group, Crystal growth study, Culture medium, Demineralized water, Detection reagent, Differentiation marker, Estrogen, Extracellular matrix protein, Ftir, High concentrations, Higher concentrations, Implant, Implant osseointegration, Liquid chromatograph, Mater, Microgram protein, Mouse osteocalcin, Octacalcium phosphate, Online issue, Oral maxillofac implants, Original article, Osteoblast, Osteoblast response, Osteoblastic differentiation, Osteocalcin, Osteocalcin production, Osteogenesis, Osteogenic differentiation, Osteogenic effects, Porous titanium implants, Porous titanium surfaces, Porous titanium surfaces yang, Proliferation experiment, Protein content, Puerariae radix, Puerarin, Puerarin concentration, Room temperature, Scanning electron microscope, Standard medium, Statistical difference, Stimulatory effect, Stomatology hospital, Surface characteristics, Test groups, Titanium, Titanium disks, Titanium implants, Titanium plates, Titanium surfaces, Total protein, Wash buffer, Wiley periodicals, Zhejiang, Zhejiang guangci, Zhejiang university.
- Teeft :
- Assay, Assay solution, Biomed mater, Biomedical materials research, Biomimetic, Biomimetic calcium phosphate coating, Biomimetic coatings, Bone formation, Bone loss, Cell attachment, Cell culture medium sample, Cell lysate, Cellular protein, Cellytic buffer, Chemical compositions, Collagen, Collagen synthesis, Collagen synthesis assay, Collagen type, Contract grant number, Contract grant sponsor, Control group, Crystal growth study, Culture medium, Demineralized water, Detection reagent, Differentiation marker, Estrogen, Extracellular matrix protein, Ftir, High concentrations, Higher concentrations, Implant, Implant osseointegration, Liquid chromatograph, Mater, Microgram protein, Mouse osteocalcin, Octacalcium phosphate, Online issue, Oral maxillofac implants, Original article, Osteoblast, Osteoblast response, Osteoblastic differentiation, Osteocalcin, Osteocalcin production, Osteogenesis, Osteogenic differentiation, Osteogenic effects, Porous titanium implants, Porous titanium surfaces, Porous titanium surfaces yang, Proliferation experiment, Protein content, Puerariae radix, Puerarin, Puerarin concentration, Room temperature, Scanning electron microscope, Standard medium, Statistical difference, Stimulatory effect, Stomatology hospital, Surface characteristics, Test groups, Titanium, Titanium disks, Titanium implants, Titanium plates, Titanium surfaces, Total protein, Wash buffer, Wiley periodicals, Zhejiang, Zhejiang guangci, Zhejiang university.
Abstract
Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
Url:
DOI: 10.1002/jbm.a.34080
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<front><div type="abstract">Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.</div>
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