Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study
Identifieur interne : 000A42 ( Istex/Corpus ); précédent : 000A41; suivant : 000A43Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study
Auteurs : Fan Yang ; Rong Zhang ; Fuming He ; Xiao-Xiang Wang ; Shifang Zhao ; Guoli YangSource :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2012-06.
English descriptors
- KwdEn :
- Assay, Assay solution, Biomed mater, Biomedical materials research, Biomimetic, Biomimetic calcium phosphate coating, Biomimetic coatings, Bone formation, Bone loss, Cell attachment, Cell culture medium sample, Cell lysate, Cellular protein, Cellytic buffer, Chemical compositions, Collagen, Collagen synthesis, Collagen synthesis assay, Collagen type, Contract grant number, Contract grant sponsor, Control group, Crystal growth study, Culture medium, Demineralized water, Detection reagent, Differentiation marker, Estrogen, Extracellular matrix protein, Ftir, High concentrations, Higher concentrations, Implant, Implant osseointegration, Liquid chromatograph, Mater, Microgram protein, Mouse osteocalcin, Octacalcium phosphate, Online issue, Oral maxillofac implants, Original article, Osteoblast, Osteoblast response, Osteoblastic differentiation, Osteocalcin, Osteocalcin production, Osteogenesis, Osteogenic differentiation, Osteogenic effects, Porous titanium implants, Porous titanium surfaces, Porous titanium surfaces yang, Proliferation experiment, Protein content, Puerariae radix, Puerarin, Puerarin concentration, Room temperature, Scanning electron microscope, Standard medium, Statistical difference, Stimulatory effect, Stomatology hospital, Surface characteristics, Test groups, Titanium, Titanium disks, Titanium implants, Titanium plates, Titanium surfaces, Total protein, Wash buffer, Wiley periodicals, Zhejiang, Zhejiang guangci, Zhejiang university.
- Teeft :
- Assay, Assay solution, Biomed mater, Biomedical materials research, Biomimetic, Biomimetic calcium phosphate coating, Biomimetic coatings, Bone formation, Bone loss, Cell attachment, Cell culture medium sample, Cell lysate, Cellular protein, Cellytic buffer, Chemical compositions, Collagen, Collagen synthesis, Collagen synthesis assay, Collagen type, Contract grant number, Contract grant sponsor, Control group, Crystal growth study, Culture medium, Demineralized water, Detection reagent, Differentiation marker, Estrogen, Extracellular matrix protein, Ftir, High concentrations, Higher concentrations, Implant, Implant osseointegration, Liquid chromatograph, Mater, Microgram protein, Mouse osteocalcin, Octacalcium phosphate, Online issue, Oral maxillofac implants, Original article, Osteoblast, Osteoblast response, Osteoblastic differentiation, Osteocalcin, Osteocalcin production, Osteogenesis, Osteogenic differentiation, Osteogenic effects, Porous titanium implants, Porous titanium surfaces, Porous titanium surfaces yang, Proliferation experiment, Protein content, Puerariae radix, Puerarin, Puerarin concentration, Room temperature, Scanning electron microscope, Standard medium, Statistical difference, Stimulatory effect, Stomatology hospital, Surface characteristics, Test groups, Titanium, Titanium disks, Titanium implants, Titanium plates, Titanium surfaces, Total protein, Wash buffer, Wiley periodicals, Zhejiang, Zhejiang guangci, Zhejiang university.
Abstract
Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
Url:
DOI: 10.1002/jbm.a.34080
Links to Exploration step
ISTEX:14E8BF4FAEFDC43323233A0517E3A9C96B32D94DLe document en format XML
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water</term><term>Detection reagent</term><term>Differentiation marker</term><term>Estrogen</term><term>Extracellular matrix protein</term><term>Ftir</term><term>High concentrations</term><term>Higher concentrations</term><term>Implant</term><term>Implant osseointegration</term><term>Liquid chromatograph</term><term>Mater</term><term>Microgram protein</term><term>Mouse osteocalcin</term><term>Octacalcium phosphate</term><term>Online issue</term><term>Oral maxillofac implants</term><term>Original article</term><term>Osteoblast</term><term>Osteoblast response</term><term>Osteoblastic differentiation</term><term>Osteocalcin</term><term>Osteocalcin production</term><term>Osteogenesis</term><term>Osteogenic differentiation</term><term>Osteogenic effects</term><term>Porous titanium implants</term><term>Porous titanium surfaces</term><term>Porous titanium surfaces yang</term><term>Proliferation experiment</term><term>Protein content</term><term>Puerariae 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sample</term><term>Cell lysate</term><term>Cellular protein</term><term>Cellytic buffer</term><term>Chemical compositions</term><term>Collagen</term><term>Collagen synthesis</term><term>Collagen synthesis assay</term><term>Collagen type</term><term>Contract grant number</term><term>Contract grant sponsor</term><term>Control group</term><term>Crystal growth study</term><term>Culture medium</term><term>Demineralized water</term><term>Detection reagent</term><term>Differentiation marker</term><term>Estrogen</term><term>Extracellular matrix protein</term><term>Ftir</term><term>High concentrations</term><term>Higher concentrations</term><term>Implant</term><term>Implant osseointegration</term><term>Liquid chromatograph</term><term>Mater</term><term>Microgram protein</term><term>Mouse osteocalcin</term><term>Octacalcium phosphate</term><term>Online issue</term><term>Oral maxillofac implants</term><term>Original article</term><term>Osteoblast</term><term>Osteoblast 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guangci</term><term>Zhejiang university</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. 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concentrations</json:string><json:string>surface characteristics</json:string><json:string>osteocalcin production</json:string><json:string>microgram protein</json:string><json:string>scanning electron microscope</json:string><json:string>differentiation marker</json:string><json:string>osteogenic effects</json:string><json:string>titanium implants</json:string><json:string>oral maxillofac implants</json:string><json:string>puerariae radix</json:string><json:string>biomimetic coatings</json:string><json:string>zhejiang guangci</json:string><json:string>mater</json:string></teeft></keywords><author><json:item><name>Fan Yang</name><affiliations><json:string>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China</json:string></affiliations></json:item><json:item><name>Rong Zhang</name><affiliations><json:string>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China</json:string></affiliations></json:item><json:item><name>Fuming He</name><affiliations><json:string>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China</json:string></affiliations></json:item><json:item><name>Xiao‐Xiang Wang</name><affiliations><json:string>Department of Materials Science and Engineering, Zhejiang University, Zheda Road, Hangzhou, China</json:string></affiliations></json:item><json:item><name>Shifang Zhao</name><affiliations><json:string>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</json:string></affiliations></json:item><json:item><name>Guoli Yang</name><affiliations><json:string>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</json:string><json:string>E-mail: guo_li1977@yahoo.cn</json:string><json:string>Correspondence address: Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>puerarin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>osteoblast</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>octacalcium phosphate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>titanium</value></json:item></subject><articleId><json:string>JBM34080</json:string></articleId><arkIstex>ark:/67375/WNG-F4P3WKCQ-J</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p > 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.</abstract><qualityIndicators><score>9.44</score><pdfWordCount>4440</pdfWordCount><pdfCharCount>28305</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>612 x 810 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>260</abstractWordCount><abstractCharCount>1864</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study</title><pmid><json:string>22374837</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Biomedical Materials Research Part A</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1552-4965</json:string></doi><issn><json:string>1549-3296</json:string></issn><eissn><json:string>1552-4965</json:string></eissn><publisherId><json:string>JBM</json:string></publisherId><volume>100A</volume><issue>6</issue><pages><first>1419</first><last>1426</last><total>8</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>2012</json:string></date><geogName></geogName><orgName><json:string>WILEY PERIODICALS, INC.</json:string><json:string>Zhejiang University</json:string><json:string>Agilent Technologies</json:string><json:string>Biomedical Technologies</json:string><json:string>Zhejiang Provincial Traditional Chinese Medicine Foundation</json:string><json:string>Department of Oral and Maxillofacial Surgery</json:string><json:string>China Department of Materials Science and Engineering</json:string><json:string>Zhejiang Guangci Medical Appliance Co., Ningbo, China</json:string><json:string>Health Department for Excellent Youth Talents</json:string><json:string>Stomatology Hospital, School of Medical, Zhejiang University, Yan</json:string><json:string>Wiley Periodicals, Inc</json:string><json:string>University of Toronto, Canada</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Zhejiang Guangci</json:string><json:string>Professor Sean</json:string><json:string>G. Yang</json:string></persName><placeName><json:string>IL</json:string><json:string>Hangzhou</json:string><json:string>China</json:string><json:string>Chicago</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Wong et al.</json:string><json:string>Zhang et al.</json:string><json:string>Huh et al.</json:string><json:string>Pettersson et al.</json:string><json:string>An et al.</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-F4P3WKCQ-J</json:string></ark><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1002/jbm.a.34080</json:string></doi><id>14E8BF4FAEFDC43323233A0517E3A9C96B32D94D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/14E8BF4FAEFDC43323233A0517E3A9C96B32D94D/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/14E8BF4FAEFDC43323233A0517E3A9C96B32D94D/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/14E8BF4FAEFDC43323233A0517E3A9C96B32D94D/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Osteoblast response to puerarin‐loaded porous titanium surfaces: An <hi rend="italic">in vitro</hi> study<ref type="note" target="#fn1"></ref></title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><licence>Copyright © 2012 Wiley Periodicals, Inc.</licence></availability><date type="published" when="2012-06"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main" xml:lang="en">Osteoblast response to puerarin‐loaded porous titanium surfaces: An <hi rend="italic">in vitro</hi> study<ref type="note" target="#fn1"></ref></title><title level="a" type="short" xml:lang="en">Osteoblast Response to Puerarin‐Loaded Porous Titanium Surfaces</title><author xml:id="author-0000"><persName><forename type="first">Fan</forename><surname>Yang</surname></persName><affiliation>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China<address><country key="CN"></country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">Rong</forename><surname>Zhang</surname></persName><affiliation>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China<address><country key="CN"></country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Fuming</forename><surname>He</surname></persName><affiliation>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China<address><country key="CN"></country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Xiao‐Xiang</forename><surname>Wang</surname></persName><affiliation>Department of Materials Science and Engineering, Zhejiang University, Zheda Road, Hangzhou, China<address><country key="CN"></country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Shifang</forename><surname>Zhao</surname></persName><affiliation>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China<address><country key="CN"></country></address></affiliation></author><author xml:id="author-0005" role="corresp"><persName><forename type="first">Guoli</forename><surname>Yang</surname></persName><email>guo_li1977@yahoo.cn</email><affiliation>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China<address><country key="CN"></country></address></affiliation><affiliation>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</affiliation></author><idno type="istex">14E8BF4FAEFDC43323233A0517E3A9C96B32D94D</idno><idno type="ark">ark:/67375/WNG-F4P3WKCQ-J</idno><idno type="DOI">10.1002/jbm.a.34080</idno><idno type="unit">JBM34080</idno><idno type="toTypesetVersion">file:JBM.JBM34080.pdf</idno></analytic><monogr><title level="j" type="main">Journal of Biomedical Materials Research Part A</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title><idno type="pISSN">1549-3296</idno><idno type="eISSN">1552-4965</idno><idno type="book-DOI">10.1002/(ISSN)1552-4965</idno><idno type="book-part-DOI">10.1002/jbm.a.v100a.6</idno><idno type="product">JBM</idno><imprint><biblScope unit="vol">100A</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1419">1419</biblScope><biblScope unit="page" to="1426">1426</biblScope><biblScope unit="page-count">8</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2012-06"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><head>Abstract</head><p>Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1<hi rend="bold">)</hi>. Puerarin was prepared onto titanium surfaces with varying concentration (10<hi rend="superscript">−9</hi><hi rend="italic">M</hi>, 10<hi rend="superscript">−8</hi><hi rend="italic">M</hi>, 10<hi rend="superscript">−7</hi><hi rend="italic">M</hi>, and 10<hi rend="superscript">−6</hi><hi rend="italic">M</hi>) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10<hi rend="superscript">−8</hi><hi rend="italic">M</hi> group was 10.22 ± 0.32 ng/cm<hi rend="superscript">2</hi>. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10<hi rend="superscript">−8</hi> and 10<hi rend="superscript">−7</hi><hi rend="italic">M</hi> groups on day 4, 10<hi rend="superscript">−8</hi>, 10<hi rend="superscript">−7</hi>, and 10<hi rend="superscript">−6</hi><hi rend="italic">M</hi> groups on day 7, and 10<hi rend="superscript">−8</hi> on day 14. In Type I collagen synthesis assay, 10<hi rend="superscript">−9</hi> and 10<hi rend="superscript">−8</hi><hi rend="italic">M</hi> on day 7, 10<hi rend="superscript">−8</hi> on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (<hi rend="italic">p</hi> < 0.05, at 10<hi rend="superscript">−8</hi><hi rend="italic">M</hi> and 10<hi rend="superscript">−7</hi><hi rend="italic">M</hi>, maximal at 10<hi rend="superscript">−8</hi><hi rend="italic">M</hi>). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">puerarin</term><term xml:id="kwd2">osteoblast</term><term xml:id="kwd3">octacalcium phosphate</term><term xml:id="kwd4">titanium</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/14E8BF4FAEFDC43323233A0517E3A9C96B32D94D/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1552-4965</doi><issn type="print">1549-3296</issn><issn type="electronic">1552-4965</issn><idGroup><id type="product" value="JBM"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A">Journal of Biomedical Materials Research Part A</title><title type="short">J. 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Res.</title></titleGroup><selfCitationGroup><citation type="ancestor" xml:id="cit1"><journalTitle>Journal of Biomedical Materials Research</journalTitle><accessionId ref="info:x-wiley/issn/00219304">0021-9304</accessionId><accessionId ref="info:x-wiley/issn/10974636">1097-4636</accessionId><pubYear year="2004">2004</pubYear></citation></selfCitationGroup></publicationMeta><publicationMeta level="part" position="60"><doi origin="wiley" registered="yes">10.1002/jbm.a.v100a.6</doi><numberingGroup><numbering type="journalVolume" number="100">100A</numbering><numbering type="journalIssue">6</numbering></numberingGroup><coverDate startDate="2012-06">June 2012</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="50" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jbm.a.34080</doi><idGroup><id type="unit" value="JBM34080"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><copyright ownership="publisher">Copyright © 2012 Wiley Periodicals, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="2011-12-14"></event><event type="manuscriptAccepted" date="2012-01-06"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:3.1.3 standalone mode:FullText" date="2012-04-11"></event><event type="publishedOnlineEarlyUnpaginated" date="2012-02-28"></event><event type="firstOnline" date="2012-02-28"></event><event type="publishedOnlineFinalForm" date="2012-04-11"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.3.4 mode:FullText" date="2015-02-24"></event></eventGroup><numberingGroup><numbering type="pageFirst">1419</numbering><numbering type="pageLast">1426</numbering></numberingGroup><correspondenceTo>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JBM.JBM34080.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="8"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="32"></count><count type="wordTotal" number="5350"></count></countGroup><titleGroup><title type="main" xml:lang="en">Osteoblast response to puerarin‐loaded porous titanium surfaces: An <i>in vitro</i> study<link href="#fn1"></link></title><title type="short" xml:lang="en">Osteoblast Response to Puerarin‐Loaded Porous Titanium Surfaces</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Fan</givenNames><familyName>Yang</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Rong</givenNames><familyName>Zhang</familyName></personName></creator><creator xml:id="au3" 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countryCode="CN" type="organization"><unparsedAffiliation>Department of Materials Science and Engineering, Zhejiang University, Zheda Road, Hangzhou, China</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="CN" type="organization"><unparsedAffiliation>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">puerarin</keyword><keyword xml:id="kwd2">osteoblast</keyword><keyword xml:id="kwd3">octacalcium phosphate</keyword><keyword xml:id="kwd4">titanium</keyword></keywordGroup><fundingInfo><fundingAgency>Zhejiang Provincial Medical and Health Department for Excellent Youth Talents</fundingAgency><fundingNumber>2009QN019</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Zhejiang Provincial Traditional Chinese Medicine Foundation</fundingAgency><fundingNumber>2010ZB082</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1<b>)</b>. Puerarin was prepared onto titanium surfaces with varying concentration (10<sup>−9</sup><i>M</i>, 10<sup>−8</sup><i>M</i>, 10<sup>−7</sup><i>M</i>, and 10<sup>−6</sup><i>M</i>) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10<sup>−8</sup><i>M</i> group was 10.22 ± 0.32 ng/cm<sup>2</sup>. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10<sup>−8</sup> and 10<sup>−7</sup><i>M</i> groups on day 4, 10<sup>−8</sup>, 10<sup>−7</sup>, and 10<sup>−6</sup><i>M</i> groups on day 7, and 10<sup>−8</sup> on day 14. In Type I collagen synthesis assay, 10<sup>−9</sup> and 10<sup>−8</sup><i>M</i> on day 7, 10<sup>−8</sup> on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (<i>p</i> < 0.05, at 10<sup>−8</sup><i>M</i> and 10<sup>−7</sup><i>M</i>, maximal at 10<sup>−8</sup><i>M</i>). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p><b>How to cite this article</b>: Yang F, Zhang R, He F, Wang X‐X, Zhao S, Yang G. 2012. Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study. J Biomed Mater Res Part A 2012:100A:1419–1426.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Osteoblast Response to Puerarin‐Loaded Porous Titanium Surfaces</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study</title></titleInfo><name type="personal"><namePart type="given">Fan</namePart><namePart type="family">Yang</namePart><affiliation>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rong</namePart><namePart type="family">Zhang</namePart><affiliation>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fuming</namePart><namePart type="family">He</namePart><affiliation>Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xiao‐Xiang</namePart><namePart type="family">Wang</namePart><affiliation>Department of Materials Science and Engineering, Zhejiang University, Zheda Road, Hangzhou, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shifang</namePart><namePart type="family">Zhao</namePart><affiliation>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Guoli</namePart><namePart type="family">Yang</namePart><affiliation>Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</affiliation><affiliation>E-mail: guo_li1977@yahoo.cn</affiliation><affiliation>Correspondence address: Department of Oral and maxillofacial surgery, Stomatology Hospital, School of Medical, Zhejiang University, Yan'an Road, Hangzhou 310006, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2012-06</dateIssued><dateCaptured encoding="w3cdtf">2011-12-14</dateCaptured><dateValid encoding="w3cdtf">2012-01-06</dateValid><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">8</extent><extent unit="tables">1</extent><extent unit="references">32</extent><extent unit="words">5350</extent></physicalDescription><abstract>Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.</abstract><note type="content">*How to cite this article: Yang F, Zhang R, He F, Wang X‐X, Zhao S, Yang G. 2012. Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study. J Biomed Mater Res Part A 2012:100A:1419–1426.</note><note type="funding">Zhejiang Provincial Medical and Health Department for Excellent Youth Talents - No. 2009QN019; </note><note type="funding">Zhejiang Provincial Traditional Chinese Medicine Foundation - No. 2010ZB082; </note><subject lang="en"><genre>keywords</genre><topic>puerarin</topic><topic>osteoblast</topic><topic>octacalcium phosphate</topic><topic>titanium</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Biomedical Materials Research Part A</title></titleInfo><titleInfo type="abbreviated"><title>J. Biomed. Mater. 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