Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis
Identifieur interne : 002817 ( Istex/Corpus ); précédent : 002816; suivant : 002818Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis
Auteurs : Stephen A. Martin ; William A. Falkler Jr ; Jon B. Suzuici ; Charles E. Hawley ; Bruce F. MacklerSource :
- Journal of Periodontal Research [ 0022-3484 ] ; 1986-07.
English descriptors
- KwdEn :
- Adult periodontitis, Antibody levels, Asaccharolyticus, Asaecharolyticus, Assay, Autologous, Autologous sera, Baap, Bacteroides, Bacteroides gingivalis, Bacteroides melaninogenicus, Bgap, Bgrp, Carbonate buffer, Correlation coefficient, Dental research, Elisa, Falkler, Fusobacterium nucleatum, Gahi, Genco, Genus bacteroides, Gingival, Gingival tissue, Gingivalis, Immunoglobulin, Immunoglobulin levels, Immunoglobulin levels reactive, Immunoglobulin reactivity, Immunoglobulins reactive, Immunologic, Juvenile periodontitis, Lesion, Localized, Lymphocyte, Microbiology, Microorganism, Mouton, Optical density, Oral bacteria, Oral biology, Page schroeder, Patient groups, Patients reactive, Periodontal, Periodontal disease, Periodontal diseases, Periodontal research, Periodontitis, Periodontitis patients, Periodontology, Plasma cells, Reactive, Real differences, Significant difference, Significant differences, Slots genco, Socransky, Sodium dodecyl sulfate, Supernatant fluids, Tissue culture medium.
- Teeft :
- Adult periodontitis, Antibody levels, Asaccharolyticus, Asaecharolyticus, Assay, Autologous, Autologous sera, Baap, Bacteroides, Bacteroides gingivalis, Bacteroides melaninogenicus, Bgap, Bgrp, Carbonate buffer, Correlation coefficient, Dental research, Elisa, Falkler, Fusobacterium nucleatum, Gahi, Genco, Genus bacteroides, Gingival, Gingival tissue, Gingivalis, Immunoglobulin, Immunoglobulin levels, Immunoglobulin levels reactive, Immunoglobulin reactivity, Immunoglobulins reactive, Immunologic, Juvenile periodontitis, Lesion, Localized, Lymphocyte, Microbiology, Microorganism, Mouton, Optical density, Oral bacteria, Oral biology, Page schroeder, Patient groups, Patients reactive, Periodontal, Periodontal disease, Periodontal diseases, Periodontal research, Periodontitis, Periodontitis patients, Periodontology, Plasma cells, Reactive, Real differences, Significant difference, Significant differences, Slots genco, Socransky, Sodium dodecyl sulfate, Supernatant fluids, Tissue culture medium.
Abstract
The purpose of this study was to compare the reactivity of the localized immunoglobulins present in the supernatant fluids from in vitro gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sera of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to Bacteroides gingivalis, an organism often implicated in periodontitis, as well as B. asaccharolyticus, a non‐oral, non‐crossreacting member of the genus Bacteroides, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to B. gingivalis were significantly greater (p < 0.05) than those to B. asaccharolyticus regardless of patient group. Similarly, the AP group always exhibited significantly (p < 0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to B. gingivalis or B. asaccharolyticus when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen B. gingivalis that are not necessarily reflected in the systemic serum antibody responses of these patients.
Url:
DOI: 10.1111/j.1600-0765.1986.tb01469.x
Links to Exploration step
ISTEX:519DC7A10201E4E9A4957E06AC374B6EF0790037Le document en format XML
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Martin</name><affiliation><mods:affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Falkler Jr, William A" sort="Falkler Jr, William A" uniqKey="Falkler Jr W" first="William A." last="Falkler Jr">William A. Falkler Jr</name><affiliation><mods:affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Address:, Dr. William A. Falkler, Jr., Chairman Department of Microbiology, University of Maryland Dental School 666 West Baltimore Street, Baltimore, Maryland 21201 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Suzuici, Jon B" sort="Suzuici, Jon B" uniqKey="Suzuici J" first="Jon B." last="Suzuici">Jon B. Suzuici</name><affiliation><mods:affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Hawley, Charles E" sort="Hawley, Charles E" uniqKey="Hawley C" first="Charles E." last="Hawley">Charles E. Hawley</name><affiliation><mods:affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Mackler, Bruce F" sort="Mackler, Bruce F" uniqKey="Mackler B" first="Bruce F." last="Mackler">Bruce F. Mackler</name><affiliation><mods:affiliation>Department of Oral Pathology, University of Maryland, Baltimore, Maryland U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="ISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><imprint><biblScope unit="vol">21</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="351">351</biblScope><biblScope unit="page" to="364">364</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1986-07">1986-07</date></imprint><idno type="ISSN">0022-3484</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult periodontitis</term><term>Antibody levels</term><term>Asaccharolyticus</term><term>Asaecharolyticus</term><term>Assay</term><term>Autologous</term><term>Autologous sera</term><term>Baap</term><term>Bacteroides</term><term>Bacteroides gingivalis</term><term>Bacteroides melaninogenicus</term><term>Bgap</term><term>Bgrp</term><term>Carbonate buffer</term><term>Correlation coefficient</term><term>Dental research</term><term>Elisa</term><term>Falkler</term><term>Fusobacterium nucleatum</term><term>Gahi</term><term>Genco</term><term>Genus bacteroides</term><term>Gingival</term><term>Gingival tissue</term><term>Gingivalis</term><term>Immunoglobulin</term><term>Immunoglobulin levels</term><term>Immunoglobulin levels reactive</term><term>Immunoglobulin reactivity</term><term>Immunoglobulins reactive</term><term>Immunologic</term><term>Juvenile periodontitis</term><term>Lesion</term><term>Localized</term><term>Lymphocyte</term><term>Microbiology</term><term>Microorganism</term><term>Mouton</term><term>Optical density</term><term>Oral bacteria</term><term>Oral biology</term><term>Page schroeder</term><term>Patient groups</term><term>Patients reactive</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal diseases</term><term>Periodontal research</term><term>Periodontitis</term><term>Periodontitis patients</term><term>Periodontology</term><term>Plasma cells</term><term>Reactive</term><term>Real differences</term><term>Significant difference</term><term>Significant differences</term><term>Slots genco</term><term>Socransky</term><term>Sodium dodecyl sulfate</term><term>Supernatant fluids</term><term>Tissue culture medium</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adult periodontitis</term><term>Antibody levels</term><term>Asaccharolyticus</term><term>Asaecharolyticus</term><term>Assay</term><term>Autologous</term><term>Autologous sera</term><term>Baap</term><term>Bacteroides</term><term>Bacteroides gingivalis</term><term>Bacteroides melaninogenicus</term><term>Bgap</term><term>Bgrp</term><term>Carbonate buffer</term><term>Correlation coefficient</term><term>Dental research</term><term>Elisa</term><term>Falkler</term><term>Fusobacterium nucleatum</term><term>Gahi</term><term>Genco</term><term>Genus bacteroides</term><term>Gingival</term><term>Gingival tissue</term><term>Gingivalis</term><term>Immunoglobulin</term><term>Immunoglobulin levels</term><term>Immunoglobulin levels reactive</term><term>Immunoglobulin reactivity</term><term>Immunoglobulins reactive</term><term>Immunologic</term><term>Juvenile periodontitis</term><term>Lesion</term><term>Localized</term><term>Lymphocyte</term><term>Microbiology</term><term>Microorganism</term><term>Mouton</term><term>Optical density</term><term>Oral bacteria</term><term>Oral biology</term><term>Page schroeder</term><term>Patient groups</term><term>Patients reactive</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal diseases</term><term>Periodontal research</term><term>Periodontitis</term><term>Periodontitis patients</term><term>Periodontology</term><term>Plasma cells</term><term>Reactive</term><term>Real differences</term><term>Significant difference</term><term>Significant differences</term><term>Slots genco</term><term>Socransky</term><term>Sodium dodecyl sulfate</term><term>Supernatant fluids</term><term>Tissue culture medium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this study was to compare the reactivity of the localized immunoglobulins present in the supernatant fluids from in vitro gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sera of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to Bacteroides gingivalis, an organism often implicated in periodontitis, as well as B. asaccharolyticus, a non‐oral, non‐crossreacting member of the genus Bacteroides, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to B. gingivalis were significantly greater (p < 0.05) than those to B. asaccharolyticus regardless of patient group. Similarly, the AP group always exhibited significantly (p < 0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to B. gingivalis or B. asaccharolyticus when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen B. gingivalis that are not necessarily reflected in the systemic serum antibody responses of these patients.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>gingivalis</json:string><json:string>periodontitis</json:string><json:string>immunoglobulin</json:string><json:string>periodontal</json:string><json:string>reactive</json:string><json:string>bacteroides</json:string><json:string>asaccharolyticus</json:string><json:string>gingival</json:string><json:string>elisa</json:string><json:string>genco</json:string><json:string>periodontal disease</json:string><json:string>autologous</json:string><json:string>gingival tissue</json:string><json:string>asaecharolyticus</json:string><json:string>microorganism</json:string><json:string>periodontal research</json:string><json:string>periodontology</json:string><json:string>gahi</json:string><json:string>autologous sera</json:string><json:string>falkler</json:string><json:string>baap</json:string><json:string>lymphocyte</json:string><json:string>adult periodontitis</json:string><json:string>immunologic</json:string><json:string>bgrp</json:string><json:string>dental research</json:string><json:string>socransky</json:string><json:string>microbiology</json:string><json:string>mouton</json:string><json:string>bgap</json:string><json:string>immunoglobulin levels reactive</json:string><json:string>slots genco</json:string><json:string>bacteroides gingivalis</json:string><json:string>immunoglobulin levels</json:string><json:string>correlation coefficient</json:string><json:string>assay</json:string><json:string>localized</json:string><json:string>carbonate buffer</json:string><json:string>optical density</json:string><json:string>supernatant fluids</json:string><json:string>patients reactive</json:string><json:string>oral biology</json:string><json:string>page schroeder</json:string><json:string>plasma cells</json:string><json:string>immunoglobulins reactive</json:string><json:string>antibody levels</json:string><json:string>bacteroides melaninogenicus</json:string><json:string>periodontitis patients</json:string><json:string>tissue culture medium</json:string><json:string>real differences</json:string><json:string>immunoglobulin reactivity</json:string><json:string>genus bacteroides</json:string><json:string>oral bacteria</json:string><json:string>significant differences</json:string><json:string>sodium dodecyl sulfate</json:string><json:string>significant difference</json:string><json:string>fusobacterium nucleatum</json:string><json:string>patient groups</json:string><json:string>juvenile periodontitis</json:string><json:string>periodontal diseases</json:string><json:string>lesion</json:string></teeft></keywords><author><json:item><name>Stephen A. Martin</name><affiliations><json:string>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</json:string></affiliations></json:item><json:item><name>William A. Falkler Jr</name><affiliations><json:string>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</json:string><json:string>Correspondence address: Address:, Dr. William A. Falkler, Jr., Chairman Department of Microbiology, University of Maryland Dental School 666 West Baltimore Street, Baltimore, Maryland 21201 U.S.A.</json:string></affiliations></json:item><json:item><name>Jon B. Suzuici</name><affiliations><json:string>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</json:string><json:string>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</json:string></affiliations></json:item><json:item><name>Charles E. Hawley</name><affiliations><json:string>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</json:string><json:string>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</json:string></affiliations></json:item><json:item><name>Bruce F. Mackler</name><affiliations><json:string>Department of Oral Pathology, University of Maryland, Baltimore, Maryland U.S.A.</json:string></affiliations></json:item></author><articleId><json:string>JRE351</json:string></articleId><arkIstex>ark:/67375/WNG-XNN39X23-P</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The purpose of this study was to compare the reactivity of the localized immunoglobulins present in the supernatant fluids from in vitro gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sera of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to Bacteroides gingivalis, an organism often implicated in periodontitis, as well as B. asaccharolyticus, a non‐oral, non‐crossreacting member of the genus Bacteroides, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to B. gingivalis were significantly greater (p > 0.05) than those to B. asaccharolyticus regardless of patient group. Similarly, the AP group always exhibited significantly (p > 0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to B. gingivalis or B. asaccharolyticus when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen B. gingivalis that are not necessarily reflected in the systemic serum antibody responses of these patients.</abstract><qualityIndicators><score>10</score><pdfWordCount>5989</pdfWordCount><pdfCharCount>39904</pdfCharCount><pdfVersion>1.6</pdfVersion><pdfPageCount>15</pdfPageCount><pdfPageSize>450 x 668 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>266</abstractWordCount><abstractCharCount>1795</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis</title><pmid><json:string>2942663</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Periodontal Research</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1600-0765</json:string></doi><issn><json:string>0022-3484</json:string></issn><eissn><json:string>1600-0765</json:string></eissn><publisherId><json:string>JRE</json:string></publisherId><volume>21</volume><issue>4</issue><pages><first>351</first><last>364</last><total>14</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1986</json:string></date><geogName></geogName><orgName><json:string>Fisher Scientific Co.</json:string><json:string>-Department of Periodontics</json:string><json:string>University of Maryland Dental School</json:string><json:string>Valley Biologies, Inc.</json:string><json:string>Department of Oral Pathology, University of Maryland, Baltimore, Maryland U</json:string><json:string>Eastman Kodak Co.</json:string><json:string>Aldrich Chemical Co.</json:string><json:string>Vacutainer Systems</json:string><json:string>Sigma Chemical Co.</json:string><json:string>Coy Laboratory Products, Inc</json:string><json:string>Johnson Products, Inc.</json:string><json:string>Flow Laboratories, Inc.</json:string><json:string>American Type Culture Collection</json:string><json:string>Dynatech Laboratories</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Joy Cook</json:string><json:string>W. A. Falkler</json:string><json:string>Elaine Romberg</json:string><json:string>ELISA Microorganism</json:string><json:string>Judy Pennington</json:string></persName><placeName><json:string>Rochester</json:string><json:string>Milwaukee</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Slots & Genco 1984</json:string><json:string>Suzuki et al. 1984</json:string><json:string>Syed et al. 1980</json:string><json:string>Giavind & Loe 1967</json:string><json:string>Newman et al. 1976</json:string><json:string>Slots 1982</json:string><json:string>Accepted for publication January 3, 1986</json:string><json:string>Doty et al. 1982</json:string><json:string>Mangan, Won & Lopatin 1983</json:string><json:string>Tanner et al. 1979</json:string><json:string>Falkler et al. 1982</json:string><json:string>Schei et al. 1959</json:string><json:string>Mouton et al. 1981</json:string><json:string>Spiegel et al. 1979</json:string><json:string>Loe & Silness 1963</json:string><json:string>Mackler et al. (1977)</json:string><json:string>Mouton et al. 1980</json:string><json:string>Tolo, Schenck & Johansen 1982</json:string><json:string>Steubing et al. 1982</json:string><json:string>Page & Schroeder 1982</json:string><json:string>Mouton et al. 1980, 1981</json:string><json:string>Patters et al. 1976, 1980</json:string><json:string>Page et al. (1983)</json:string><json:string>Lehner et al. 1974</json:string><json:string>Lowry et al. 1951</json:string><json:string>Silness & Loe 1964</json:string><json:string>Lowry et al. (1951)</json:string><json:string>Mackler et al. 1978</json:string><json:string>Reed et al. 1980</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-XNN39X23-P</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - dentistry, oral surgery & medicine</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - dentistry</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Dentistry</json:string><json:string>3 - Periodontics</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - pathologie infectieuse</json:string></inist></categories><publicationDate>1986</publicationDate><copyrightDate>1986</copyrightDate><doi><json:string>10.1111/j.1600-0765.1986.tb01469.x</json:string></doi><id>519DC7A10201E4E9A4957E06AC374B6EF0790037</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/519DC7A10201E4E9A4957E06AC374B6EF0790037/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/519DC7A10201E4E9A4957E06AC374B6EF0790037/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/519DC7A10201E4E9A4957E06AC374B6EF0790037/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1986-07"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis</title><author xml:id="author-0000"><persName><forename type="first">Stephen A.</forename><surname>Martin</surname></persName><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation></author><author xml:id="author-0001" role="corresp"><persName><genName>Jr</genName><forename type="first">William A.</forename><surname>Falkler</surname></persName><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation><affiliation>Address:, Dr. William A. Falkler, Jr., Chairman Department of Microbiology, University of Maryland Dental School 666 West Baltimore Street, Baltimore, Maryland 21201 U.S.A.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Jon B.</forename><surname>Suzuici</surname></persName><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation><affiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Charles E.</forename><surname>Hawley</surname></persName><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation><affiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Bruce F.</forename><surname>Mackler</surname></persName><affiliation>Department of Oral Pathology, University of Maryland, Baltimore, Maryland U.S.A.<address><country key="US"></country></address></affiliation></author><idno type="istex">519DC7A10201E4E9A4957E06AC374B6EF0790037</idno><idno type="ark">ark:/67375/WNG-XNN39X23-P</idno><idno type="DOI">10.1111/j.1600-0765.1986.tb01469.x</idno><idno type="unit">JRE351</idno><idno type="toTypesetVersion">file:JRE.JRE351.pdf</idno></analytic><monogr><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="pISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><idno type="book-DOI">10.1111/(ISSN)1600-0765</idno><idno type="book-part-DOI">10.1111/jre.1986.21.issue-4</idno><idno type="product">JRE</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">21</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="351">351</biblScope><biblScope unit="page" to="364">364</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1986-07"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><p>The purpose of this study was to compare the reactivity of the localized immunoglobulins present in the supernatant fluids from <hi rend="italic">in vitro</hi> gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sera of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to <hi rend="italic">Bacteroides gingivalis</hi>, an organism often implicated in periodontitis, as well as <hi rend="italic">B. asaccharolyticus</hi>, a non‐oral, non‐crossreacting member of the genus <hi rend="italic">Bacteroides</hi>, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to <hi rend="italic">B. gingivalis</hi> were significantly greater (p < 0.05) than those to <hi rend="italic">B. asaccharolyticus</hi> regardless of patient group. Similarly, the AP group always exhibited significantly (p < 0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to <hi rend="italic">B. gingivalis</hi> or <hi rend="italic">B. asaccharolyticus</hi> when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen <hi rend="italic">B. gingivalis</hi> that are not necessarily reflected in the systemic serum antibody responses of these patients.</p></abstract><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/519DC7A10201E4E9A4957E06AC374B6EF0790037/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1600-0765</doi><issn type="print">0022-3484</issn><issn type="electronic">1600-0765</issn><idGroup><id type="product" value="JRE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF PERIODONTAL RESEARCH">Journal of Periodontal Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="07004"><doi origin="wiley">10.1111/jre.1986.21.issue-4</doi><numberingGroup><numbering type="journalVolume" number="21">21</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="1986-07">July 1986</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0035100" status="forIssue"><doi origin="wiley">10.1111/j.1600-0765.1986.tb01469.x</doi><idGroup><id type="unit" value="JRE351"></id></idGroup><countGroup><count type="pageTotal" number="14"></count></countGroup><eventGroup><event type="firstOnline" date="2006-06-30"></event><event type="publishedOnlineFinalForm" date="2006-06-30"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="351">351</numbering><numbering type="pageLast" number="364">364</numbering></numberingGroup><correspondenceTo>Address:, Dr. William A. Falkler, Jr., Chairman Department of Microbiology, University of Maryland Dental School 666 West Baltimore Street, Baltimore, Maryland 21201 U.S.A.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JRE.JRE351.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>(Accepted for publication January 3, 1986)</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="57"></count><count type="linksCrossRef" number="8"></count></countGroup><titleGroup><title type="main">Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Stephen A.</givenNames><familyName>Martin</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" corresponding="yes"><personName><givenNames>William A.</givenNames><familyName>Falkler</familyName><nameSuffix>Jr</nameSuffix></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1 #a2"><personName><givenNames>Jon B.</givenNames><familyName>Suzuici</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1 #a2"><personName><givenNames>Charles E.</givenNames><familyName>Hawley</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>Bruce F.</givenNames><familyName>Mackler</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US"><unparsedAffiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="US"><unparsedAffiliation>Department of Oral Pathology, University of Maryland, Baltimore, Maryland U.S.A.</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The purpose of this study was to compare the reactivity of the localized immunoglobulins present in the supernatant fluids from <i>in vitro</i> gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sera of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to <i>Bacteroides gingivalis</i>, an organism often implicated in periodontitis, as well as <i>B. asaccharolyticus</i>, a non‐oral, non‐crossreacting member of the genus <i>Bacteroides</i>, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to <i>B. gingivalis</i> were significantly greater (p < 0.05) than those to <i>B. asaccharolyticus</i> regardless of patient group. Similarly, the AP group always exhibited significantly (p < 0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to <i>B. gingivalis</i> or <i>B. asaccharolyticus</i> when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen <i>B. gingivalis</i> that are not necessarily reflected in the systemic serum antibody responses of these patients.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Local and systemic immunoglobulins reactive to Bacteroides gingivalis in rapidly progressive and adult periodontitis</title></titleInfo><name type="personal"><namePart type="given">Stephen A.</namePart><namePart type="family">Martin</namePart><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William A.</namePart><namePart type="family">Falkler Jr</namePart><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><affiliation>Correspondence address: Address:, Dr. William A. Falkler, Jr., Chairman Department of Microbiology, University of Maryland Dental School 666 West Baltimore Street, Baltimore, Maryland 21201 U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jon B.</namePart><namePart type="family">Suzuici</namePart><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><affiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Charles E.</namePart><namePart type="family">Hawley</namePart><affiliation>Department of Microbiology, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><affiliation>Department of Periodontics, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bruce F.</namePart><namePart type="family">Mackler</namePart><affiliation>Department of Oral Pathology, University of Maryland, Baltimore, Maryland U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1986-07</dateIssued><edition>(Accepted for publication January 3, 1986)</edition><copyrightDate encoding="w3cdtf">1986</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">57</extent><extent unit="linksCrossRef">8</extent></physicalDescription><abstract lang="en">The purpose of this study was to compare the reactivity of the localized immunoglobulins present in the supernatant fluids from in vitro gingival organ cultures of rapidly progressive (RP) and adult periodontitis (AP) patients with those immunoglobulins occurring systemically in the sera of these patients. Tissue excised from periodontitis patients was diagnosed and classified as being either RP or AP and placed in the wells of a multiwell microtiter plate to which RPMI 1640 tissue culture medium was added. Supernatant fluids were harvested and fresh culture medium added to the wells at 24 h intervals. Supernatant fluids and autologous sera from RP and AP patients were tested for reactivity to Bacteroides gingivalis, an organism often implicated in periodontitis, as well as B. asaccharolyticus, a non‐oral, non‐crossreacting member of the genus Bacteroides, utilizing an ELISA technique. When immunoglobulins present in gingival tissue supernatant fluids were examined, it was found that mean levels reactive to B. gingivalis were significantly greater (p < 0.05) than those to B. asaccharolyticus regardless of patient group. Similarly, the AP group always exhibited significantly (p < 0.05) increased antibody reactivity to B. gingivalis when compared to the RP periodontitis patients. However, when autologous sera from these patients were assayed, no significant differences were observed for immunoglobulin levels reactive to B. gingivalis or B. asaccharolyticus when comparing the AP and RP patient groups, or within the AP group alone. The results indicate that differences exist in the localized immunological responses of RP and AP patients to the periodontopathogen B. gingivalis that are not necessarily reflected in the systemic serum antibody responses of these patients.</abstract><relatedItem type="host"><titleInfo><title>Journal of Periodontal Research</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0022-3484</identifier><identifier type="eISSN">1600-0765</identifier><identifier type="DOI">10.1111/(ISSN)1600-0765</identifier><identifier type="PublisherID">JRE</identifier><part><date>1986</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>351</start><end>364</end><total>14</total></extent></part></relatedItem><identifier type="istex">519DC7A10201E4E9A4957E06AC374B6EF0790037</identifier><identifier type="ark">ark:/67375/WNG-XNN39X23-P</identifier><identifier type="DOI">10.1111/j.1600-0765.1986.tb01469.x</identifier><identifier type="ArticleID">JRE351</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/519DC7A10201E4E9A4957E06AC374B6EF0790037/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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