Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Identifieur interne : 000757 ( Pmc/Curation ); précédent : 000756; suivant : 000758Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Auteurs : Willian F. Zambuzzi [Brésil] ; Estevam A. Bonfante [Brésil] ; Ryo Jimbo [Suède] ; Mariko Hayashi [Suède] ; Martin Andersson [Suède] ; Gutemberg Alves [Brésil] ; Esther R. Takamori [Brésil] ; Paulo J. Beltrão [Brésil] ; Paulo G. Coelho [États-Unis] ; José M. Granjeiro [Brésil]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.
Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.
The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.
It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.
Url:
DOI: 10.1371/journal.pone.0095662
PubMed: 24999733
PubMed Central: 4085036
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Coelho</name><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York</wicri:regionArea></affiliation></author><author><name sortKey="Granjeiro, Jose M" sort="Granjeiro, Jose M" uniqKey="Granjeiro J" first="José M." last="Granjeiro">José M. Granjeiro</name><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24999733</idno><idno type="pmc">4085036</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085036</idno><idno type="RBID">PMC:4085036</idno><idno type="doi">10.1371/journal.pone.0095662</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000757</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000757</idno><idno type="wicri:Area/Pmc/Curation">000757</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000757</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations</title><author><name sortKey="Zambuzzi, Willian F" sort="Zambuzzi, Willian F" uniqKey="Zambuzzi W" first="Willian F." last="Zambuzzi">Willian F. Zambuzzi</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Departmento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departmento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo</wicri:regionArea></affiliation></author><author><name sortKey="Bonfante, Estevam A" sort="Bonfante, Estevam A" uniqKey="Bonfante E" first="Estevam A." last="Bonfante">Estevam A. Bonfante</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo</wicri:regionArea></affiliation></author><author><name sortKey="Jimbo, Ryo" sort="Jimbo, Ryo" uniqKey="Jimbo R" first="Ryo" last="Jimbo">Ryo Jimbo</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea></affiliation></author><author><name sortKey="Hayashi, Mariko" sort="Hayashi, Mariko" uniqKey="Hayashi M" first="Mariko" last="Hayashi">Mariko Hayashi</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea></affiliation></author><author><name sortKey="Andersson, Martin" sort="Andersson, Martin" uniqKey="Andersson M" first="Martin" last="Andersson">Martin Andersson</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Department of Chemical and Biological Engineering, Applied Surface Chemistry, Chalmers University of Technology, Gothenburg, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemical and Biological Engineering, Applied Surface Chemistry, Chalmers University of Technology, Gothenburg</wicri:regionArea></affiliation></author><author><name sortKey="Alves, Gutemberg" sort="Alves, Gutemberg" uniqKey="Alves G" first="Gutemberg" last="Alves">Gutemberg Alves</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Department of Cell and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niteroi, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Cell and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niteroi</wicri:regionArea></affiliation></author><author><name sortKey="Takamori, Esther R" sort="Takamori, Esther R" uniqKey="Takamori E" first="Esther R." last="Takamori">Esther R. Takamori</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Excellion Biomedical Services, Petrópolis, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Excellion Biomedical Services, Petrópolis, Rio de Janeiro</wicri:regionArea></affiliation></author><author><name sortKey="Beltrao, Paulo J" sort="Beltrao, Paulo J" uniqKey="Beltrao P" first="Paulo J." last="Beltrão">Paulo J. Beltrão</name><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro</wicri:regionArea></affiliation></author><author><name sortKey="Coelho, Paulo G" sort="Coelho, Paulo G" uniqKey="Coelho P" first="Paulo G." last="Coelho">Paulo G. Coelho</name><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York</wicri:regionArea></affiliation></author><author><name sortKey="Granjeiro, Jose M" sort="Granjeiro, Jose M" uniqKey="Granjeiro J" first="José M." last="Granjeiro">José M. Granjeiro</name><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.</p></sec><sec><title>Methodology</title><p>Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.</p></sec><sec><title>Principal Findings</title><p>The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.</p></sec><sec><title>Conclusions</title><p>It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chuang, Sk" uniqKey="Chuang S">SK Chuang</name></author><author><name sortKey="Cai, T" uniqKey="Cai T">T Cai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Esposito, M" uniqKey="Esposito M">M Esposito</name></author><author><name sortKey="Murray Curtis, L" uniqKey="Murray Curtis L">L Murray-Curtis</name></author><author><name 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24999733</article-id><article-id pub-id-type="pmc">4085036</article-id><article-id pub-id-type="publisher-id">PONE-D-13-29671</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0095662</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Physical Sciences</subject><subj-group><subject>Chemistry</subject><subj-group><subject>Chemical Properties</subject><subj-group><subject>Physicochemical Properties</subject></subj-group></subj-group><subj-group><subject>Physical Chemistry</subject></subj-group></subj-group><subj-group><subject>Materials Science</subject><subj-group><subject>Material Properties</subject><subject>Materials Characterization</subject><subject>Materials Design</subject><subject>Metallurgy</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology and Life Sciences</subject><subj-group><subject>Biotechnology</subject><subj-group><subject>Biomaterials</subject></subj-group></subj-group><subj-group><subject>Cell Biology</subject><subj-group><subject>Cell Processes</subject><subj-group><subject>Cell Growth</subject></subj-group></subj-group><subj-group><subject>Cell Adhesion</subject><subject>Molecular Cell Biology</subject></subj-group></subj-group><subj-group><subject>Genetics</subject><subj-group><subject>Gene Expression</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations</article-title><alt-title alt-title-type="running-head">Signal Transduction and Titanium Surface Texturing</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zambuzzi</surname><given-names>Willian F.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bonfante</surname><given-names>Estevam A.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jimbo</surname><given-names>Ryo</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hayashi</surname><given-names>Mariko</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Andersson</surname><given-names>Martin</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Alves</surname><given-names>Gutemberg</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Takamori</surname><given-names>Esther R.</given-names></name><xref ref-type="aff" rid="aff6"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Beltrão</surname><given-names>Paulo J.</given-names></name><xref ref-type="aff" rid="aff7"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Coelho</surname><given-names>Paulo G.</given-names></name><xref ref-type="aff" rid="aff8"><sup>8</sup></xref></contrib><contrib contrib-type="author"><name><surname>Granjeiro</surname><given-names>José M.</given-names></name><xref ref-type="aff" rid="aff7"><sup>7</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Departmento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo, Brazil</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo, Brazil</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Department of Chemical and Biological Engineering, Applied Surface Chemistry, Chalmers University of Technology, Gothenburg, Sweden</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Department of Cell and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niteroi, Brazil</addr-line></aff><aff id="aff6"><label>6</label><addr-line>Excellion Biomedical Services, Petrópolis, Rio de Janeiro, Brazil</addr-line></aff><aff id="aff7"><label>7</label><addr-line>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil</addr-line></aff><aff id="aff8"><label>8</label><addr-line>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Egles</surname><given-names>Christophe</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Université de Technologie de Compiègne, France</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>wzambuzzi@ibb.unesp.br</email> (WFZ); <email>jmgranjeiro@inmetro.gov.br</email> (JMG)</corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: WFZ EAB JMG ERT. Performed the experiments: WFZ EAB RJ MH MA GA PJB. Analyzed the data: WFZ EAB GA PJB RJ MH MA ERT. Contributed reagents/materials/analysis tools: WFZ PGC JMG. Wrote the paper: WFZ ERT PGC GA.</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>7</day><month>7</month><year>2014</year></pub-date><volume>9</volume><issue>7</issue><elocation-id>e95662</elocation-id><history><date date-type="received"><day>21</day><month>7</month><year>2013</year></date><date date-type="accepted"><day>30</day><month>3</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Zambuzzi et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><sec><title>Background</title><p>It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.</p></sec><sec><title>Methodology</title><p>Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.</p></sec><sec><title>Principal Findings</title><p>The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.</p></sec><sec><title>Conclusions</title><p>It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.</p></sec></abstract><funding-group><funding-statement>This study was funded by Fapesp, CNPq and Faperj. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="11"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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