Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Identifieur interne : 000F45 ( Ncbi/Checkpoint ); précédent : 000F44; suivant : 000F46Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Auteurs : Willian F. Zambuzzi [Brésil] ; Estevam A. Bonfante [Brésil] ; Ryo Jimbo [Suède] ; Mariko Hayashi [Suède] ; Martin Andersson [Suède] ; Gutemberg Alves [Brésil] ; Esther R. Takamori [Brésil] ; Paulo J. Beltrão [Brésil] ; Paulo G. Coelho [États-Unis] ; José M. Granjeiro [Brésil]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.
Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.
The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.
It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.
Url:
DOI: 10.1371/journal.pone.0095662
PubMed: 24999733
PubMed Central: 4085036
Affiliations:
- Brésil, Suède, États-Unis
- État de New York, État de Rio de Janeiro, État de São Paulo
- Rio de Janeiro, São Paulo
- Université de São Paulo
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Coelho</name><affiliation wicri:level="2"><nlm:aff id="aff8"><addr-line>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Granjeiro, Jose M" sort="Granjeiro, Jose M" uniqKey="Granjeiro J" first="José M." last="Granjeiro">José M. 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Zambuzzi</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Departmento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departmento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, São Paulo</wicri:regionArea><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Bonfante, Estevam A" sort="Bonfante, Estevam A" uniqKey="Bonfante E" first="Estevam A." last="Bonfante">Estevam A. Bonfante</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, São Paulo</wicri:regionArea><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region><settlement type="city">São Paulo</settlement></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Jimbo, Ryo" sort="Jimbo, Ryo" uniqKey="Jimbo R" first="Ryo" last="Jimbo">Ryo Jimbo</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Hayashi, Mariko" sort="Hayashi, Mariko" uniqKey="Hayashi M" first="Mariko" last="Hayashi">Mariko Hayashi</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Andersson, Martin" sort="Andersson, Martin" uniqKey="Andersson M" first="Martin" last="Andersson">Martin Andersson</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Department of Chemical and Biological Engineering, Applied Surface Chemistry, Chalmers University of Technology, Gothenburg, Sweden</addr-line></nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemical and Biological Engineering, Applied Surface Chemistry, Chalmers University of Technology, Gothenburg</wicri:regionArea><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Alves, Gutemberg" sort="Alves, Gutemberg" uniqKey="Alves G" first="Gutemberg" last="Alves">Gutemberg Alves</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Department of Cell and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niteroi, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Cell and Molecular Biology, Institute of Biology, Universidade Federal Fluminense, Niteroi</wicri:regionArea><wicri:noRegion>Niteroi</wicri:noRegion></affiliation></author><author><name sortKey="Takamori, Esther R" sort="Takamori, Esther R" uniqKey="Takamori E" first="Esther R." last="Takamori">Esther R. Takamori</name><affiliation wicri:level="3"><nlm:aff id="aff6"><addr-line>Excellion Biomedical Services, Petrópolis, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Excellion Biomedical Services, Petrópolis, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Beltrao, Paulo J" sort="Beltrao, Paulo J" uniqKey="Beltrao P" first="Paulo J." last="Beltrão">Paulo J. Beltrão</name><affiliation wicri:level="3"><nlm:aff id="aff7"><addr-line>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Coelho, Paulo G" sort="Coelho, Paulo G" uniqKey="Coelho P" first="Paulo G." last="Coelho">Paulo G. Coelho</name><affiliation wicri:level="2"><nlm:aff id="aff8"><addr-line>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomaterials and Biomimetics/Director for Research Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Granjeiro, Jose M" sort="Granjeiro, Jose M" uniqKey="Granjeiro J" first="José M." last="Granjeiro">José M. Granjeiro</name><affiliation wicri:level="3"><nlm:aff id="aff7"><addr-line>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>National Institute of Metrology, Quality and Technology - INMETRO, Xerém, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.</p></sec><sec><title>Methodology</title><p>Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.</p></sec><sec><title>Principal Findings</title><p>The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.</p></sec><sec><title>Conclusions</title><p>It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chuang, Sk" uniqKey="Chuang S">SK Chuang</name></author><author><name sortKey="Cai, T" uniqKey="Cai T">T Cai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Esposito, M" uniqKey="Esposito M">M Esposito</name></author><author><name sortKey="Murray Curtis, L" uniqKey="Murray Curtis L">L Murray-Curtis</name></author><author><name 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sort="Zambuzzi, Willian F" uniqKey="Zambuzzi W" first="Willian F." last="Zambuzzi">Willian F. Zambuzzi</name></region><name sortKey="Alves, Gutemberg" sort="Alves, Gutemberg" uniqKey="Alves G" first="Gutemberg" last="Alves">Gutemberg Alves</name><name sortKey="Beltrao, Paulo J" sort="Beltrao, Paulo J" uniqKey="Beltrao P" first="Paulo J." last="Beltrão">Paulo J. Beltrão</name><name sortKey="Bonfante, Estevam A" sort="Bonfante, Estevam A" uniqKey="Bonfante E" first="Estevam A." last="Bonfante">Estevam A. Bonfante</name><name sortKey="Granjeiro, Jose M" sort="Granjeiro, Jose M" uniqKey="Granjeiro J" first="José M." last="Granjeiro">José M. Granjeiro</name><name sortKey="Takamori, Esther R" sort="Takamori, Esther R" uniqKey="Takamori E" first="Esther R." last="Takamori">Esther R. Takamori</name></country><country name="Suède"><noRegion><name sortKey="Jimbo, Ryo" sort="Jimbo, Ryo" uniqKey="Jimbo R" first="Ryo" last="Jimbo">Ryo Jimbo</name></noRegion><name sortKey="Andersson, Martin" sort="Andersson, Martin" uniqKey="Andersson M" first="Martin" last="Andersson">Martin Andersson</name><name sortKey="Hayashi, Mariko" sort="Hayashi, Mariko" uniqKey="Hayashi M" first="Mariko" last="Hayashi">Mariko Hayashi</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Coelho, Paulo G" sort="Coelho, Paulo G" uniqKey="Coelho P" first="Paulo G." last="Coelho">Paulo G. Coelho</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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