Patient-specific analysis of periodontal and peri-implant microbiomes.
Identifieur interne : 001019 ( Main/Exploration ); précédent : 001018; suivant : 001020Patient-specific analysis of periodontal and peri-implant microbiomes.
Auteurs : S M Dabdoub [États-Unis] ; A A Tsigarida ; P S KumarSource :
- Journal of dental research [ 1544-0591 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ARN bactérien (analyse), ARN ribosomique 16S (analyse), Analyse de séquence d'ADN, Anaérobiose, Bactéries anaérobies à Gram négatif (), Bactéries à Gram positif (), Biodiversité, Biofilms, Chimère (génétique), Humains, Implants dentaires (microbiologie), Maladies parodontales (microbiologie), Microbiote (génétique), Mâchoire partiellement édentée (microbiologie), Mâchoire partiellement édentée (rééducation et réadaptation), Parodonte (microbiologie), Parodontite (microbiologie), Plaque dentaire (microbiologie), Péri-implantite (microbiologie), Stomatite (microbiologie), Écosystème.
- MESH :
- analyse : ADN bactérien, ARN bactérien, ARN ribosomique 16S.
- génétique : Chimère, Microbiote.
- microbiologie : Implants dentaires, Maladies parodontales, Mâchoire partiellement édentée, Parodonte, Parodontite, Plaque dentaire, Péri-implantite, Stomatite.
- rééducation et réadaptation : Mâchoire partiellement édentée.
- Analyse de séquence d'ADN, Anaérobiose, Bactéries anaérobies à Gram négatif, Bactéries à Gram positif, Biodiversité, Biofilms, Humains, Écosystème.
English descriptors
- KwdEn :
- Anaerobiosis, Biodiversity, Biofilms, Chimera (genetics), DNA, Bacterial (analysis), Dental Implants (microbiology), Dental Plaque (microbiology), Ecosystem, Gram-Negative Anaerobic Bacteria (classification), Gram-Positive Bacteria (classification), Humans, Jaw, Edentulous, Partially (microbiology), Jaw, Edentulous, Partially (rehabilitation), Microbiota (genetics), Peri-Implantitis (microbiology), Periodontal Diseases (microbiology), Periodontitis (microbiology), Periodontium (microbiology), RNA, Bacterial (analysis), RNA, Ribosomal, 16S (analysis), Sequence Analysis, DNA, Stomatitis (microbiology).
- MESH :
- chemical , analysis : DNA, Bacterial, RNA, Bacterial, RNA, Ribosomal, 16S.
- classification : Gram-Negative Anaerobic Bacteria, Gram-Positive Bacteria.
- genetics : Chimera, Microbiota.
- chemical , microbiology : Dental Implants, Dental Plaque, Jaw, Edentulous, Partially, Peri-Implantitis, Periodontal Diseases, Periodontitis, Periodontium, Stomatitis.
- rehabilitation : Jaw, Edentulous, Partially.
- Anaerobiosis, Biodiversity, Biofilms, Ecosystem, Humans, Sequence Analysis, DNA.
Abstract
Periodontally involved teeth have been implicated as 'microbial reservoirs' in the etiology of peri-implant diseases. Therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the degree of congruence between adjacent peri-implant and periodontal microbiomes in states of health and disease. Subgingival and peri-implant biofilm samples were collected from 81 partially edentulous individuals with periodontal and peri-implant health and disease. Bacterial DNA was isolated, and the 16S rRNA gene was amplified and sequenced by pyrotag sequencing. Chimera-depleted sequences were compared against a locally hosted curated database for bacterial identification. Statistical significance was determined by paired Student's t tests between tooth-implant pairs. The 1.9 million sequences identified represented 523 species. Sixty percent of individuals shared less than 50% of all species between their periodontal and peri-implant biofilms, and 85% of individuals shared less than 8% of abundant species between tooth and implant. Additionally, the periodontal microbiome demonstrated significantly higher diversity than the implant, and distinct bacterial lineages were associated with health and disease in each ecosystem. Analysis of our data suggests that simple geographic proximity is not a sufficient determinant of colonization of topographically distinct niches, and that the peri-implant and periodontal microbiomes represent microbiologically distinct ecosystems.
DOI: 10.1177/0022034513504950
PubMed: 24158341
Affiliations:
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Le document en format XML
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positif</term><term>Biodiversité</term><term>Biofilms</term><term>Humains</term><term>Écosystème</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Periodontally involved teeth have been implicated as 'microbial reservoirs' in the etiology of peri-implant diseases. Therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the degree of congruence between adjacent peri-implant and periodontal microbiomes in states of health and disease. Subgingival and peri-implant biofilm samples were collected from 81 partially edentulous individuals with periodontal and peri-implant health and disease. Bacterial DNA was isolated, and the 16S rRNA gene was amplified and sequenced by pyrotag sequencing. Chimera-depleted sequences were compared against a locally hosted curated database for bacterial identification. Statistical significance was determined by paired Student's t tests between tooth-implant pairs. The 1.9 million sequences identified represented 523 species. Sixty percent of individuals shared less than 50% of all species between their periodontal and peri-implant biofilms, and 85% of individuals shared less than 8% of abundant species between tooth and implant. Additionally, the periodontal microbiome demonstrated significantly higher diversity than the implant, and distinct bacterial lineages were associated with health and disease in each ecosystem. Analysis of our data suggests that simple geographic proximity is not a sufficient determinant of colonization of topographically distinct niches, and that the peri-implant and periodontal microbiomes represent microbiologically distinct ecosystems.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><noCountry><name sortKey="Kumar, P S" sort="Kumar, P S" uniqKey="Kumar P" first="P S" last="Kumar">P S Kumar</name><name sortKey="Tsigarida, A A" sort="Tsigarida, A A" uniqKey="Tsigarida A" first="A A" last="Tsigarida">A A Tsigarida</name></noCountry><country name="États-Unis"><region name="Ohio"><name sortKey="Dabdoub, S M" sort="Dabdoub, S M" uniqKey="Dabdoub S" first="S M" last="Dabdoub">S M Dabdoub</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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