Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus
Identifieur interne : 000672 ( Pmc/Corpus ); précédent : 000671; suivant : 000673Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus
Auteurs : Anqi Yang ; Lei Wei ; Weiran Zhao ; Yuanyuan Xu ; Zihe RaoSource :
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications [ 1744-3091 ] ; 2009.
Abstract
The N-terminal domain of nsp2 from avian infectious bronchitis virus has been purified and crystallized. The crystals diffracted to 2.5 Å resolution.
Url:
DOI: 10.1107/S1744309109024749
PubMed: 19652340
PubMed Central: 2720334
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Acta Crystallogr Sect F Struct Biol Cryst Commun</journal-id><journal-id journal-id-type="publisher-id">Acta Cryst. F</journal-id><journal-title-group><journal-title>Acta Crystallographica Section F: Structural Biology and Crystallization Communications</journal-title></journal-title-group><issn pub-type="epub">1744-3091</issn><publisher><publisher-name>International Union of Crystallography</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19652340</article-id><article-id pub-id-type="pmc">2720334</article-id><article-id pub-id-type="publisher-id">fw5224</article-id><article-id pub-id-type="doi">10.1107/S1744309109024749</article-id><article-id pub-id-type="coden">ACSFCL</article-id><article-id pub-id-type="pii">S1744309109024749</article-id><article-categories><subj-group subj-group-type="heading"><subject>Crystallization Communications</subject></subj-group></article-categories><title-group><article-title>Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus</article-title><alt-title>N-terminal domain of nsp2 from avian infectious bronchitis virus</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Anqi</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Wei</surname><given-names>Lei</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Weiran</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Yuanyuan</given-names></name><xref ref-type="aff" rid="a">a</xref><xref ref-type="corresp" rid="cor">*</xref></contrib><contrib contrib-type="author"><name><surname>Rao</surname><given-names>Zihe</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><aff id="a"><label>a</label>Tsinghua–Nankai–IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084,<country>People’s Republic of China</country></aff></contrib-group><author-notes><corresp id="cor">Correspondence e-mail: <email>xuyy@mail.xtal.tsinghua.edu.cn </email></corresp></author-notes><pub-date pub-type="collection"><day>1</day><month>8</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>25</day><month>7</month><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>8</month><year>2011</year></pub-date><pmc-comment> PMC Release delay is 24 months and 0 days and was based on the
<volume>65</volume><issue>Pt 8</issue><issue-id pub-id-type="publisher-id">f090800</issue-id><fpage>788</fpage><lpage>790</lpage><history><date date-type="received"><day>21</day><month>5</month><year>2009</year></date><date date-type="accepted"><day>26</day><month>6</month><year>2009</year></date></history><permissions><copyright-statement>© International Union of Crystallography 2009</copyright-statement><copyright-year>2009</copyright-year></permissions><self-uri xlink:type="simple" xlink:href="http://dx.doi.org/10.1107/S1744309109024749">A full version of this article is available from Crystallography Journals Online.</self-uri><abstract abstract-type="toc"><p>The N-terminal domain of nsp2 from avian infectious bronchitis virus has been purified and crystallized. The crystals diffracted to 2.5 Å resolution.</p></abstract><abstract><p>Avian infectious bronchitis virus (IBV) is a prototype of the group III coronaviruses and encodes 15 nonstructural proteins which make up the transcription/replication machinery. The nsp2 protein from IBV has a unique and novel sequence and has no experimentally confirmed function in replication, whereas it has been proposed to be crucial for early viral infection and may inhibit the early host immune response. The gene that encodes a double-mutant IBV nsp2 N-terminal domain (residues 9–393 of the polyprotein, with mutations Q132L and L270F) was cloned and expressed in <italic>Escherichia coli</italic> and the protein was subjected to crystallization trials. The crystals diffracted to 2.5 Å resolution and belonged to space group <italic>P</italic>6<sub>2</sub> or <italic>P</italic>6<sub>4</sub>, with unit-cell parameters <italic>a</italic> = <italic>b</italic> = 114.2, <italic>c</italic> = 61.0 Å, α = β = 90, γ = 120°. Each asymmetric unit contained one molecule.</p></abstract><kwd-group><kwd>coronaviruses</kwd><kwd>avian infectious bronchitis virus</kwd><kwd>nonstructural proteins</kwd><kwd>nsp2</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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