Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus
Identifieur interne : 000671 ( Pmc/Corpus ); précédent : 000670; suivant : 000672Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus
Auteurs : Kun Yu ; Zhenhua Ming ; Yuanyuan Li ; Cheng Chen ; Zehua Bao ; Zhilin Ren ; Bofeng Liu ; Wei Tao ; Zihe Rao ; Zhiyong LouSource :
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications [ 1744-3091 ] ; 2012.
Abstract
Nonstructural protein 2 from avian infectious bronchitis virus has been overexpressed in
Url:
DOI: 10.1107/S1744309112018623
PubMed: 22684079
PubMed Central: 3370919
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus</title><author><name sortKey="Yu, Kun" sort="Yu, Kun" uniqKey="Yu K" first="Kun" last="Yu">Kun Yu</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Ming, Zhenhua" sort="Ming, Zhenhua" uniqKey="Ming Z" first="Zhenhua" last="Ming">Zhenhua Ming</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Li, Yuanyuan" sort="Li, Yuanyuan" uniqKey="Li Y" first="Yuanyuan" last="Li">Yuanyuan Li</name><affiliation><nlm:aff id="b">National Laboratory of Macromolecules, Institute of Biophysics,<institution>Chinese Academy of Science</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Chen, Cheng" sort="Chen, Cheng" uniqKey="Chen C" first="Cheng" last="Chen">Cheng Chen</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Bao, Zehua" sort="Bao, Zehua" uniqKey="Bao Z" first="Zehua" last="Bao">Zehua Bao</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Ren, Zhilin" sort="Ren, Zhilin" uniqKey="Ren Z" first="Zhilin" last="Ren">Zhilin Ren</name><affiliation><nlm:aff id="c">College of Life Sciences and Tianjin State Laboratory of Protein Science,<institution>Nankai University</institution>, Tianjin,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Liu, Bofeng" sort="Liu, Bofeng" uniqKey="Liu B" first="Bofeng" last="Liu">Bofeng Liu</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Tao, Wei" sort="Tao, Wei" uniqKey="Tao W" first="Wei" last="Tao">Wei Tao</name><affiliation><nlm:aff id="d">College of Life Science,<institution>Hunan Normal University</institution>, Changsha,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Lou, Zhiyong" sort="Lou, Zhiyong" uniqKey="Lou Z" first="Zhiyong" last="Lou">Zhiyong Lou</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22684079</idno><idno type="pmc">3370919</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3370919</idno><idno type="RBID">PMC:3370919</idno><idno type="doi">10.1107/S1744309112018623</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000671</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000671</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus</title><author><name sortKey="Yu, Kun" sort="Yu, Kun" uniqKey="Yu K" first="Kun" last="Yu">Kun Yu</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Ming, Zhenhua" sort="Ming, Zhenhua" uniqKey="Ming Z" first="Zhenhua" last="Ming">Zhenhua Ming</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Li, Yuanyuan" sort="Li, Yuanyuan" uniqKey="Li Y" first="Yuanyuan" last="Li">Yuanyuan Li</name><affiliation><nlm:aff id="b">National Laboratory of Macromolecules, Institute of Biophysics,<institution>Chinese Academy of Science</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Chen, Cheng" sort="Chen, Cheng" uniqKey="Chen C" first="Cheng" last="Chen">Cheng Chen</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Bao, Zehua" sort="Bao, Zehua" uniqKey="Bao Z" first="Zehua" last="Bao">Zehua Bao</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Ren, Zhilin" sort="Ren, Zhilin" uniqKey="Ren Z" first="Zhilin" last="Ren">Zhilin Ren</name><affiliation><nlm:aff id="c">College of Life Sciences and Tianjin State Laboratory of Protein Science,<institution>Nankai University</institution>, Tianjin,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Liu, Bofeng" sort="Liu, Bofeng" uniqKey="Liu B" first="Bofeng" last="Liu">Bofeng Liu</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Tao, Wei" sort="Tao, Wei" uniqKey="Tao W" first="Wei" last="Tao">Wei Tao</name><affiliation><nlm:aff id="d">College of Life Science,<institution>Hunan Normal University</institution>, Changsha,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author><author><name sortKey="Lou, Zhiyong" sort="Lou, Zhiyong" uniqKey="Lou Z" first="Zhiyong" last="Lou">Zhiyong Lou</name><affiliation><nlm:aff id="a">Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff></affiliation></author></analytic><series><title level="j">Acta Crystallographica Section F: Structural Biology and Crystallization Communications</title><idno type="eISSN">1744-3091</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Nonstructural protein 2 from avian infectious bronchitis virus has been overexpressed in <italic>E. coli</italic>, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Acta Crystallogr Sect F Struct Biol Cryst Commun</journal-id><journal-id journal-id-type="iso-abbrev">Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun</journal-id><journal-id journal-id-type="publisher-id">Acta Cryst. F</journal-id><journal-title-group><journal-title>Acta Crystallographica Section F: Structural Biology and Crystallization Communications</journal-title></journal-title-group><issn pub-type="epub">1744-3091</issn><publisher><publisher-name>International Union of Crystallography</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22684079</article-id><article-id pub-id-type="pmc">3370919</article-id><article-id pub-id-type="publisher-id">ft5022</article-id><article-id pub-id-type="doi">10.1107/S1744309112018623</article-id><article-id pub-id-type="coden">ACSFCL</article-id><article-id pub-id-type="pii">S1744309112018623</article-id><article-categories><subj-group subj-group-type="heading"><subject>Crystallization Communications</subject></subj-group></article-categories><title-group><article-title>Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus</article-title><alt-title>Nsp2</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yu</surname><given-names>Kun</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Ming</surname><given-names>Zhenhua</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Yuanyuan</given-names></name><xref ref-type="aff" rid="b">b</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Cheng</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Bao</surname><given-names>Zehua</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Ren</surname><given-names>Zhilin</given-names></name><xref ref-type="aff" rid="c">c</xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Bofeng</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Tao</surname><given-names>Wei</given-names></name><xref ref-type="aff" rid="d">d</xref></contrib><contrib contrib-type="author"><name><surname>Rao</surname><given-names>Zihe</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Lou</surname><given-names>Zhiyong</given-names></name><xref ref-type="aff" rid="a">a</xref><xref ref-type="corresp" rid="cor">*</xref></contrib><aff id="a"><label>a</label>Structural Biology Laboratory and MOE Laboratory of Protein Science, School of Medicine and Life Science,<institution>Tsinghua University</institution>, Beijing,<country>People’s Republic of China</country></aff><aff id="b"><label>b</label>National Laboratory of Macromolecules, Institute of Biophysics,<institution>Chinese Academy of Science</institution>, Beijing,<country>People’s Republic of China</country></aff><aff id="c"><label>c</label>College of Life Sciences and Tianjin State Laboratory of Protein Science,<institution>Nankai University</institution>, Tianjin,<country>People’s Republic of China</country></aff><aff id="d"><label>d</label>College of Life Science,<institution>Hunan Normal University</institution>, Changsha,<country>People’s Republic of China</country></aff></contrib-group><author-notes><corresp id="cor">Correspondence e-mail: <email>louzy@xtal.tsinghua.edu.cn</email></corresp></author-notes><pub-date pub-type="collection"><day>1</day><month>6</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>24</day><month>5</month><year>2012</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>6</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 24 months and 0 days and was based on the
<volume>68</volume><issue>Pt 6</issue><issue-id pub-id-type="publisher-id">f120600</issue-id><fpage>716</fpage><lpage>719</lpage><history><date date-type="received"><day>08</day><month>2</month><year>2012</year></date><date date-type="accepted"><day>25</day><month>4</month><year>2012</year></date></history><permissions><copyright-statement>© International Union of Crystallography 2012</copyright-statement><copyright-year>2012</copyright-year></permissions><self-uri xlink:type="simple" xlink:href="http://dx.doi.org/10.1107/S1744309112018623">A full version of this article is available from Crystallography Journals Online.</self-uri><abstract abstract-type="toc"><p>Nonstructural protein 2 from avian infectious bronchitis virus has been overexpressed in <italic>E. coli</italic>, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.</p></abstract><abstract><p>Avian infectious bronchitis virus (IBV) is a member of the group III coronaviruses, which differ from the other groups of coronaviruses in that they do not encode the essential pathogenic factor nonstructural protein 1 (nsp1) and instead start with nsp2. IBV nsp2 is one of the first replicase proteins to be translated and processed in the viral life cycle; however, it has an entirely unknown function. In order to better understand the structural details and functional mechanism of IBV nsp2, the recombinant protein was cloned, overexpressed in <italic>Escherichia coli</italic>, purified and crystallized. The crystals diffracted to 2.8 Å resolution and belonged to space group <italic>P</italic>2<sub>1</sub>, with unit-cell parameters <italic>a</italic> = 57.0, <italic>b</italic> = 192.3, <italic>c</italic> = 105.7 Å, β = 90.8°. Two molecules were found in the asymmetric unit; the Matthews coefficient was 3.9 Å<sup>3</sup> Da<sup>−1</sup>, corresponding to a solvent content of 68.2%.</p></abstract><kwd-group><kwd>avian infectious bronchitis virus</kwd><kwd>nonstructural protein 2</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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