Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles
Identifieur interne : 000420 ( Pmc/Checkpoint ); précédent : 000419; suivant : 000421Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles
Auteurs : Cintia Hiromi Okino [Brésil] ; Marcos Antônio Zanella Mores [Brésil] ; Iara Maria Trevisol [Brésil] ; Arlei Coldebella [Brésil] ; Hélio José Montassier [Brésil] ; Liana Brentano [Brésil]Source :
- PLoS ONE [ 1932-6203 ] ; 2017.
Abstract
Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.
Url:
DOI: 10.1371/journal.pone.0172275
PubMed: 28199419
PubMed Central: 5310907
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:5310907Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles</title><author><name sortKey="Okino, Cintia Hiromi" sort="Okino, Cintia Hiromi" uniqKey="Okino C" first="Cintia Hiromi" last="Okino">Cintia Hiromi Okino</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Mores, Marcos Antonio Zanella" sort="Mores, Marcos Antonio Zanella" uniqKey="Mores M" first="Marcos Antônio Zanella" last="Mores">Marcos Antônio Zanella Mores</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Trevisol, Iara Maria" sort="Trevisol, Iara Maria" uniqKey="Trevisol I" first="Iara Maria" last="Trevisol">Iara Maria Trevisol</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Coldebella, Arlei" sort="Coldebella, Arlei" uniqKey="Coldebella A" first="Arlei" last="Coldebella">Arlei Coldebella</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Montassier, Helio Jose" sort="Montassier, Helio Jose" uniqKey="Montassier H" first="Hélio José" last="Montassier">Hélio José Montassier</name><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Laboratory of Immunology and Virology (Imunovir), Department of Veterinary Pathology, Universidade Estadual Paulista—UNESP, Jaboticabal, SP, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Immunology and Virology (Imunovir), Department of Veterinary Pathology, Universidade Estadual Paulista—UNESP, Jaboticabal, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Brentano, Liana" sort="Brentano, Liana" uniqKey="Brentano L" first="Liana" last="Brentano">Liana Brentano</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28199419</idno><idno type="pmc">5310907</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310907</idno><idno type="RBID">PMC:5310907</idno><idno type="doi">10.1371/journal.pone.0172275</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000988</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000988</idno><idno type="wicri:Area/Pmc/Curation">000988</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000988</idno><idno type="wicri:Area/Pmc/Checkpoint">000420</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000420</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles</title><author><name sortKey="Okino, Cintia Hiromi" sort="Okino, Cintia Hiromi" uniqKey="Okino C" first="Cintia Hiromi" last="Okino">Cintia Hiromi Okino</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Mores, Marcos Antonio Zanella" sort="Mores, Marcos Antonio Zanella" uniqKey="Mores M" first="Marcos Antônio Zanella" last="Mores">Marcos Antônio Zanella Mores</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Trevisol, Iara Maria" sort="Trevisol, Iara Maria" uniqKey="Trevisol I" first="Iara Maria" last="Trevisol">Iara Maria Trevisol</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Coldebella, Arlei" sort="Coldebella, Arlei" uniqKey="Coldebella A" first="Arlei" last="Coldebella">Arlei Coldebella</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author><author><name sortKey="Montassier, Helio Jose" sort="Montassier, Helio Jose" uniqKey="Montassier H" first="Hélio José" last="Montassier">Hélio José Montassier</name><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Laboratory of Immunology and Virology (Imunovir), Department of Veterinary Pathology, Universidade Estadual Paulista—UNESP, Jaboticabal, SP, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Immunology and Virology (Imunovir), Department of Veterinary Pathology, Universidade Estadual Paulista—UNESP, Jaboticabal, SP</wicri:regionArea><placeName><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Brentano, Liana" sort="Brentano, Liana" uniqKey="Brentano L" first="Liana" last="Brentano">Liana Brentano</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Swine and Poultry, Concórdia, SC</wicri:regionArea><placeName><region type="state">Santa Catarina</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kotani, T" uniqKey="Kotani T">T Kotani</name></author><author><name sortKey="Wada, S" uniqKey="Wada S">S Wada</name></author><author><name sortKey="Tsukamoto, Y" uniqKey="Tsukamoto Y">Y Tsukamoto</name></author><author><name sortKey="Kuwamura, M" uniqKey="Kuwamura M">M Kuwamura</name></author><author><name sortKey="Yamate, J" uniqKey="Yamate J">J Yamate</name></author><author><name sortKey="Sakuma, S" uniqKey="Sakuma S">S Sakuma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okino, Ch" uniqKey="Okino C">CH Okino</name></author><author><name sortKey="Dos Santos, Il" uniqKey="Dos Santos I">IL dos Santos</name></author><author><name sortKey="Fernando, Fs" uniqKey="Fernando F">FS Fernando</name></author><author><name sortKey="Alessi, Ac" uniqKey="Alessi A">AC Alessi</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Montassier, Hj" uniqKey="Montassier H">HJ Montassier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Franca, M" uniqKey="Franca M">M França</name></author><author><name sortKey="Woolcock, Pr" uniqKey="Woolcock P">PR Woolcock</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M Yu</name></author><author><name sortKey="Jackwood, Mw" uniqKey="Jackwood M">MW Jackwood</name></author><author><name sortKey="Shivaprasad, Hl" uniqKey="Shivaprasad H">HL Shivaprasad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cong, F" uniqKey="Cong F">F Cong</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Han, Z" uniqKey="Han Z">Z Han</name></author><author><name sortKey="Shao, Y" uniqKey="Shao Y">Y Shao</name></author><author><name sortKey="Kong, X" uniqKey="Kong X">X Kong</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gelb, J" uniqKey="Gelb J">J Gelb</name></author><author><name sortKey="Landman, Bs" uniqKey="Landman B">BS Landman</name></author><author><name sortKey="Pope, Cr" uniqKey="Pope C">CR Pope</name></author><author><name sortKey="Ruano, Jm" uniqKey="Ruano J">JM Ruano</name></author><author><name sortKey="Brannik, Em" uniqKey="Brannik E">EM Brannik</name></author><author><name sortKey="Bautista, Da" uniqKey="Bautista D">DA Bautista</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fernando, Fs" uniqKey="Fernando F">FS Fernando</name></author><author><name sortKey="Okino, Ch" uniqKey="Okino C">CH Okino</name></author><author><name sortKey="Silva, Kr" uniqKey="Silva K">KR Silva</name></author><author><name sortKey="Fernandes, Cc" uniqKey="Fernandes C">CC Fernandes</name></author><author><name sortKey="Goncalves, Mcm" uniqKey="Goncalves M">MCM Gonçalves</name></author><author><name sortKey="Montassier, Mfs" uniqKey="Montassier M">MFS Montassier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, X" uniqKey="Guo X">X Guo</name></author><author><name sortKey="Rosa, Aj" uniqKey="Rosa A">AJ Rosa</name></author><author><name sortKey="Chen, Dg" uniqKey="Chen D">DG Chen</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okino, Ch" uniqKey="Okino C">CH Okino</name></author><author><name sortKey="Alessi, Ac" uniqKey="Alessi A">AC Alessi</name></author><author><name sortKey="Montassier, Mfs" uniqKey="Montassier M">MFS Montassier</name></author><author><name sortKey="Rosa, Ajm" uniqKey="Rosa A">AJM Rosa</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Montassier, Hj" uniqKey="Montassier H">HJ Montassier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chhabra, R" uniqKey="Chhabra R">R Chhabra</name></author><author><name sortKey="Forrester, A" uniqKey="Forrester A">A Forrester</name></author><author><name sortKey="Lemiere, S" uniqKey="Lemiere S">S Lemiere</name></author><author><name sortKey="Awad, F" uniqKey="Awad F">F Awad</name></author><author><name sortKey="Chantrey, J" uniqKey="Chantrey J">J Chantrey</name></author><author><name sortKey="Ganapathy, K" uniqKey="Ganapathy K">K Ganapathy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fraga, Ap" uniqKey="Fraga A">AP Fraga</name></author><author><name sortKey="Balestrin, E" uniqKey="Balestrin E">E Balestrin</name></author><author><name sortKey="Ikuta, N" uniqKey="Ikuta N">N Ikuta</name></author><author><name sortKey="Fonseca, As" uniqKey="Fonseca A">AS Fonseca</name></author><author><name sortKey="Spilki, Fr" uniqKey="Spilki F">FR Spilki</name></author><author><name sortKey="Canal, Cw" uniqKey="Canal C">CW Canal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luo, H" uniqKey="Luo H">H Luo</name></author><author><name sortKey="Qin, J" uniqKey="Qin J">J Qin</name></author><author><name sortKey="Chen, F" uniqKey="Chen F">F Chen</name></author><author><name sortKey="Xie, Q" uniqKey="Xie Q">Q Xie</name></author><author><name sortKey="Bi, Y" uniqKey="Bi Y">Y Bi</name></author><author><name sortKey="Cao, Y" uniqKey="Cao Y">Y Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, By" uniqKey="Kim B">BY Kim</name></author><author><name sortKey="Lee, Dh" uniqKey="Lee D">DH Lee</name></author><author><name sortKey="Jang, Jh" uniqKey="Jang J">JH Jang</name></author><author><name sortKey="Lim, Th" uniqKey="Lim T">TH Lim</name></author><author><name sortKey="Choi, Sw" uniqKey="Choi S">SW Choi</name></author><author><name sortKey="Youn, Hn" uniqKey="Youn H">HN Youn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdel Moneim, As" uniqKey="Abdel Moneim A">AS Abdel-Moneim</name></author><author><name sortKey="Afifi, Ma" uniqKey="Afifi M">MA Afifi</name></author><author><name sortKey="El Kady, Mf" uniqKey="El Kady M">MF El-Kady</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Wit, Jj" uniqKey="De Wit J">JJ De Wit</name></author><author><name sortKey="Cook, Jka" uniqKey="Cook J">JKA Cook</name></author><author><name sortKey="Heijden, Hmjf" uniqKey="Heijden H">HMJF Heijden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, J" uniqKey="Smith J">J Smith</name></author><author><name sortKey="Sadeyen, Jr" uniqKey="Sadeyen J">JR Sadeyen</name></author><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author><author><name sortKey="Kaiser, P" uniqKey="Kaiser P">P Kaiser</name></author><author><name sortKey="Burt, Dw" uniqKey="Burt D">DW Burt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kameka, Am" uniqKey="Kameka A">AM Kameka</name></author><author><name sortKey="Haddadi, S" uniqKey="Haddadi S">S Haddadi</name></author><author><name sortKey="Kim, Dsk" uniqKey="Kim D">DSK Kim</name></author><author><name sortKey="Cork, Sc" uniqKey="Cork S">SC Cork</name></author><author><name sortKey="Abdul Careem, Mf" uniqKey="Abdul Careem M">MF Abdul-Careem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Rosa, Aj" uniqKey="Rosa A">AJ Rosa</name></author><author><name sortKey="Oliverira, Hn" uniqKey="Oliverira H">HN Oliverira</name></author><author><name sortKey="Rosa, Gj" uniqKey="Rosa G">GJ Rosa</name></author><author><name sortKey="Guo, X" uniqKey="Guo X">X Guo</name></author><author><name sortKey="Travnicek, M" uniqKey="Travnicek M">M Travnicek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Burggraaf, S" uniqKey="Burggraaf S">S Burggraaf</name></author><author><name sortKey="Bingham, J" uniqKey="Bingham J">J Bingham</name></author><author><name sortKey="Payne, J" uniqKey="Payne J">J Payne</name></author><author><name sortKey="Kimpton, Wg" uniqKey="Kimpton W">WG Kimpton</name></author><author><name sortKey="Lowenthal, Jw" uniqKey="Lowenthal J">JW Lowenthal</name></author><author><name sortKey="Bean, Ag" uniqKey="Bean A">AG Bean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Macmicking, J" uniqKey="Macmicking J">J MacMicking</name></author><author><name sortKey="Xie, Qw" uniqKey="Xie Q">QW Xie</name></author><author><name sortKey="Nathan, C" uniqKey="Nathan C">C Nathan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rue, Ca" uniqKey="Rue C">CA Rue</name></author><author><name sortKey="Susta, L" uniqKey="Susta L">L Susta</name></author><author><name sortKey="Cornax, I" uniqKey="Cornax I">I Cornax</name></author><author><name sortKey="Brown, Cc" uniqKey="Brown C">CC Brown</name></author><author><name sortKey="Kapczynski, Dr" uniqKey="Kapczynski D">DR Kapczynski</name></author><author><name sortKey="Suarez, Dl" uniqKey="Suarez D">DL Suarez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chhabra, R" uniqKey="Chhabra R">R Chhabra</name></author><author><name sortKey="Chantrey, J" uniqKey="Chantrey J">J Chantrey</name></author><author><name sortKey="Ganapathy, K" uniqKey="Ganapathy K">K Ganapathy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asif, M" uniqKey="Asif M">M Asif</name></author><author><name sortKey="Lowenthal, Jw" uniqKey="Lowenthal J">JW Lowenthal</name></author><author><name sortKey="Ford, Me" uniqKey="Ford M">ME Ford</name></author><author><name sortKey="Schat, Ka" uniqKey="Schat K">KA Schat</name></author><author><name sortKey="Kimpton, Wg" uniqKey="Kimpton W">WG Kimpton</name></author><author><name sortKey="Bean, Agd" uniqKey="Bean A">AGD Bean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nii, T" uniqKey="Nii T">T Nii</name></author><author><name sortKey="Isobe, N" uniqKey="Isobe N">N Isobe</name></author><author><name sortKey="Yoshimura, Y" uniqKey="Yoshimura Y">Y Yoshimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Owen, R" uniqKey="Owen R">R Owen</name></author><author><name sortKey="Cowen, Bs" uniqKey="Cowen B">BS Cowen</name></author><author><name sortKey="Hattel, A" uniqKey="Hattel A">A Hattel</name></author><author><name sortKey="Naqi, Sa" uniqKey="Naqi S">SA Naqi</name></author><author><name sortKey="Wilson, Ra" uniqKey="Wilson R">RA Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reed, Lj" uniqKey="Reed L">LJ Reed</name></author><author><name sortKey="Muench, H" uniqKey="Muench H">H Muench</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, H" uniqKey="Li H">H Li</name></author><author><name sortKey="Yang, H" uniqKey="Yang H">H Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chousalkar, Kk" uniqKey="Chousalkar K">KK Chousalkar</name></author><author><name sortKey="Cheetham, Bf" uniqKey="Cheetham B">BF Cheetham</name></author><author><name sortKey="Roberts, Jr" uniqKey="Roberts J">JR Roberts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Livak, Kj" uniqKey="Livak K">KJ Livak</name></author><author><name sortKey="Schmittgen, Td" uniqKey="Schmittgen T">TD Schmittgen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mork, Ak" uniqKey="Mork A">AK Mork</name></author><author><name sortKey="Hesse, M" uniqKey="Hesse M">M Hesse</name></author><author><name sortKey="Abd El Rahman, S" uniqKey="Abd El Rahman S">S Abd El Rahman</name></author><author><name sortKey="Rautenschlein, S" uniqKey="Rautenschlein S">S Rautenschlein</name></author><author><name sortKey="Herrler, G" uniqKey="Herrler G">G Herrler</name></author><author><name sortKey="Winter, C" uniqKey="Winter C">C Winter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chhabra, R" uniqKey="Chhabra R">R Chhabra</name></author><author><name sortKey="Kuchipudi, Sv" uniqKey="Kuchipudi S">SV Kuchipudi</name></author><author><name sortKey="Chantrey, J" uniqKey="Chantrey J">J Chantrey</name></author><author><name sortKey="Ganapathy, K" uniqKey="Ganapathy K">K Ganapathy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhong, Y" uniqKey="Zhong Y">Y Zhong</name></author><author><name sortKey="Tan, Yw" uniqKey="Tan Y">YW Tan</name></author><author><name sortKey="Liu, Dx" uniqKey="Liu D">DX Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vervelde, L" uniqKey="Vervelde L">L Vervelde</name></author><author><name sortKey="Matthijs, Mg" uniqKey="Matthijs M">MG Matthijs</name></author><author><name sortKey="Van Haarlem, Da" uniqKey="Van Haarlem D">DA van Haarlem</name></author><author><name sortKey="De Wit, Jj" uniqKey="De Wit J">JJ de Wit</name></author><author><name sortKey="Jansen, Ca" uniqKey="Jansen C">CA Jansen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28199419</article-id><article-id pub-id-type="pmc">5310907</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0172275</article-id><article-id pub-id-type="publisher-id">PONE-D-16-10083</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Birds</subject><subj-group><subject>Fowl</subject><subj-group><subject>Gamefowl</subject><subj-group><subject>Chickens</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Birds</subject><subj-group><subject>Poultry</subject><subj-group><subject>Chickens</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Livestock</subject><subj-group><subject>Poultry</subject><subj-group><subject>Chickens</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Immunology</subject><subj-group><subject>Immune Response</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Immunology</subject><subj-group><subject>Immune Response</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Respiratory System</subject><subj-group><subject>Trachea</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Respiratory System</subject><subj-group><subject>Trachea</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Genetics</subject><subj-group><subject>Gene Expression</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Genetics</subject><subj-group><subject>Genomics</subject><subj-group><subject>Animal Genomics</subject><subj-group><subject>Bird Genomics</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Renal System</subject><subj-group><subject>Kidneys</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Renal System</subject><subj-group><subject>Kidneys</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Birds</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Pathology and Laboratory Medicine</subject><subj-group><subject>Pathogenesis</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles</article-title><alt-title alt-title-type="running-head">Early immune response, pathogenesis and avian infectious bronchitis</alt-title></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0002-3692-3902</contrib-id><name><surname>Okino</surname><given-names>Cintia Hiromi</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="currentaff001"><sup>¤</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib><contrib contrib-type="author"><name><surname>Mores</surname><given-names>Marcos Antônio Zanella</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Trevisol</surname><given-names>Iara Maria</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Coldebella</surname><given-names>Arlei</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Montassier</surname><given-names>Hélio José</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Brentano</surname><given-names>Liana</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Embrapa Swine and Poultry, Concórdia, SC, Brazil</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Laboratory of Immunology and Virology (Imunovir), Department of Veterinary Pathology, Universidade Estadual Paulista—UNESP, Jaboticabal, SP, Brazil</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Jin</surname><given-names>Dong-Yan</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>University of Hong Kong, HONG KONG</addr-line></aff><author-notes><fn fn-type="COI-statement" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p><list list-type="simple"><list-item><p><bold>Conceptualization:</bold> CHO IMT LB HJM.</p></list-item><list-item><p><bold>Data curation:</bold> CHO IMT.</p></list-item><list-item><p><bold>Formal analysis:</bold> CHO IMT MAZM AC.</p></list-item><list-item><p><bold>Funding acquisition:</bold> CHO IMT LB.</p></list-item><list-item><p><bold>Investigation:</bold> CHO IMT MAZM.</p></list-item><list-item><p><bold>Methodology:</bold> CHO IMT LB.</p></list-item><list-item><p><bold>Project administration:</bold> CHO IMT.</p></list-item><list-item><p><bold>Resources:</bold> CHO IMT LB.</p></list-item><list-item><p><bold>Supervision:</bold> CHO.</p></list-item><list-item><p><bold>Validation:</bold> CHO LB.</p></list-item><list-item><p><bold>Visualization:</bold> CHO.</p></list-item><list-item><p><bold>Writing – original draft:</bold> CHO IMT MAZM AC HJM LB.</p></list-item><list-item><p><bold>Writing – review & editing:</bold> CHO HJM.</p></list-item></list></p></fn><fn fn-type="current-aff" id="currentaff001"><label>¤</label><p>Current address: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil</p></fn><corresp id="cor001">* E-mail: <email>cintia.okino@embrapa.br</email></corresp></author-notes><pub-date pub-type="epub"><day>15</day><month>2</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>12</volume><issue>2</issue><elocation-id>e0172275</elocation-id><history><date date-type="received"><day>9</day><month>3</month><year>2016</year></date><date date-type="accepted"><day>1</day><month>2</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 Okino et al</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>Okino et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0172275.pdf"></self-uri><abstract><p>Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.</p></abstract><funding-group><award-group id="award001"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/501100003046</institution-id><institution>Empresa Brasileira de Pesquisa Agropecuária</institution></institution-wrap></funding-source><award-id>03.12.03.012.0-00</award-id></award-group><funding-statement>The whole study was supported by Brazilian Agricultural Research Corporation (Embrapa), a governamental institution os research from Brazil. The funder had role in study design, data collection and analysis, decision to publish and also on the preparation of the manuscript, as most of authors (CHO, MAZM, IMT, AC and LB) work for the funder.</funding-statement></funding-group><counts><fig-count count="6"></fig-count><table-count count="3"></table-count><page-count count="17"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All data were inserted in the Results section of this manuscript, and some specific information could be found at Supporting Information.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All data were inserted in the Results section of this manuscript, and some specific information could be found at Supporting Information.</p></notes></front></pmc><affiliations><list><country><li>Brésil</li></country><region><li>Santa Catarina</li><li>État de São Paulo</li></region></list><tree><country name="Brésil"><region name="Santa Catarina"><name sortKey="Okino, Cintia Hiromi" sort="Okino, Cintia Hiromi" uniqKey="Okino C" first="Cintia Hiromi" last="Okino">Cintia Hiromi Okino</name></region><name sortKey="Brentano, Liana" sort="Brentano, Liana" uniqKey="Brentano L" first="Liana" last="Brentano">Liana Brentano</name><name sortKey="Coldebella, Arlei" sort="Coldebella, Arlei" uniqKey="Coldebella A" first="Arlei" last="Coldebella">Arlei Coldebella</name><name sortKey="Montassier, Helio Jose" sort="Montassier, Helio Jose" uniqKey="Montassier H" first="Hélio José" last="Montassier">Hélio José Montassier</name><name sortKey="Mores, Marcos Antonio Zanella" sort="Mores, Marcos Antonio Zanella" uniqKey="Mores M" first="Marcos Antônio Zanella" last="Mores">Marcos Antônio Zanella Mores</name><name sortKey="Trevisol, Iara Maria" sort="Trevisol, Iara Maria" uniqKey="Trevisol I" first="Iara Maria" last="Trevisol">Iara Maria Trevisol</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000420 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000420 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:5310907
|texte= Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:28199419" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |