Modulation of immunity and inflammatory gene expression in buffalo (Bubalus bubalis) with some digestive disorders
Identifieur interne : 000391 ( Pmc/Checkpoint ); précédent : 000390; suivant : 000392Modulation of immunity and inflammatory gene expression in buffalo (Bubalus bubalis) with some digestive disorders
Auteurs : Mohamed A. Youssef [Égypte] ; Maged R. El-Ashker [Égypte] ; Mohamed F. Ouda [Égypte]Source :
- Comparative Clinical Pathology [ 1618-5641 ] ; 2017.
Abstract
So far, there has been scarce information about the status of immunoglobulins (Ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. The purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. For this reason, 50 native breed water buffaloes were studied. Forty of these buffaloes showed various symptoms of digestive disorders and were allocated into 4 groups of equal sizes (group 1: uncategorized stomatitis; group 2: acute traumatic reticuloperitonitis [TRR]; group 3: acute rumen impaction; and group 4: undifferentiated enteritis). Ten apparently healthy buffaloes were randomly selected and considered as a control group. RNA was firstly extracted from the whole blood then a reverse transcription kits was used to convert the RNA to cDNA. Real-time PCR was used to measure the expression of mRNAs of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α, IgG, and IgA, while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as an internal reference. The results of real-time PCR revealed a significant (
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DOI: 10.1007/s00580-017-2496-1
PubMed: NONE
PubMed Central: 7088432
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Youssef</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000103426662</institution-id><institution-id institution-id-type="GRID">grid.10251.37</institution-id><institution>Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine,</institution><institution>Mansoura University,</institution></institution-wrap>Mansoura, 35516 Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Mansoura</wicri:regionArea></affiliation></author><author><name sortKey="El Ashker, Maged R" sort="El Ashker, Maged R" uniqKey="El Ashker M" first="Maged R." last="El-Ashker">Maged R. El-Ashker</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000000103426662</institution-id><institution-id institution-id-type="GRID">grid.10251.37</institution-id><institution>Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine,</institution><institution>Mansoura University,</institution></institution-wrap>Mansoura, 35516 Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Mansoura</wicri:regionArea></affiliation></author><author><name sortKey="Ouda, Mohamed F" sort="Ouda, Mohamed F" uniqKey="Ouda M" first="Mohamed F." last="Ouda">Mohamed F. Ouda</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura</wicri:regionArea><wicri:noRegion>Mansoura</wicri:noRegion></affiliation></author></analytic><series><title level="j">Comparative Clinical Pathology</title><idno type="ISSN">1618-5641</idno><idno type="eISSN">1618-565X</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">So far, there has been scarce information about the status of immunoglobulins (Ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. The purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. For this reason, 50 native breed water buffaloes were studied. Forty of these buffaloes showed various symptoms of digestive disorders and were allocated into 4 groups of equal sizes (group 1: uncategorized stomatitis; group 2: acute traumatic reticuloperitonitis [TRR]; group 3: acute rumen impaction; and group 4: undifferentiated enteritis). Ten apparently healthy buffaloes were randomly selected and considered as a control group. RNA was firstly extracted from the whole blood then a reverse transcription kits was used to convert the RNA to cDNA. Real-time PCR was used to measure the expression of mRNAs of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α, IgG, and IgA, while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as an internal reference. The results of real-time PCR revealed a significant (<italic>P</italic> ≤ 0.05) upregulation of the gene expression of IL-1β, IL-6, IL-10, and TNF-α in blood of diseased buffaloes compared with those of controls<bold>.</bold> Animals showing acute TRP had peak values of both IL-6 and IL-10; while those exhibiting enteritis and rumen impaction had the highest values of IL-1β and TNF-α, respectively. The results of qPCR also revealed a significant (<italic>P</italic> ≤ 0.05) downregulation of both IgG and IgA gene expression in blood of all diseased buffaloes compared with controls<bold>.</bold> The lowest values of both genes were recorded in buffaloes showing acute TRP. The results herein suggest that the tested genes could have a pivotal role in the pathophysiologic mechanism of the underlying diseases.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Balikci, E" uniqKey="Balikci E">E Balikci</name></author><author><name sortKey="Al, M" uniqKey="Al M">M Al</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baumann, H" uniqKey="Baumann H">H Baumann</name></author><author><name sortKey="Gauldie, J" uniqKey="Gauldie J">J Gauldie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cowan, Km" uniqKey="Cowan K">KM Cowan</name></author><author><name sortKey="Wagner, G" uniqKey="Wagner G">G Wagner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eckersall, P" uniqKey="Eckersall P">P Eckersall</name></author><author><name sortKey="Young, F" uniqKey="Young F">F 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Comp Clin Path</journal-id><journal-id journal-id-type="iso-abbrev">Comp Clin Path</journal-id><journal-title-group><journal-title>Comparative Clinical Pathology</journal-title></journal-title-group><issn pub-type="ppub">1618-5641</issn><issn pub-type="epub">1618-565X</issn><publisher><publisher-name>Springer London</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7088432</article-id><article-id pub-id-type="publisher-id">2496</article-id><article-id pub-id-type="doi">10.1007/s00580-017-2496-1</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Modulation of immunity and inflammatory gene expression in buffalo (<italic>Bubalus bubalis</italic>) with some digestive disorders</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Youssef</surname><given-names>Mohamed A.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>El-Ashker</surname><given-names>Maged R.</given-names></name><address><phone>+2-050-6329195</phone><email>maged_elashker@yahoo.com</email><email>maged.elashker1978@gmail.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ouda</surname><given-names>Mohamed F.</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="ISNI">0000000103426662</institution-id><institution-id institution-id-type="GRID">grid.10251.37</institution-id><institution>Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine,</institution><institution>Mansoura University,</institution></institution-wrap>Mansoura, 35516 Egypt</aff><aff id="Aff2"><label>2</label>Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt</aff></contrib-group><pub-date pub-type="epub"><day>31</day><month>5</month><year>2017</year></pub-date><pub-date pub-type="ppub"><year>2017</year></pub-date><volume>26</volume><issue>5</issue><fpage>1123</fpage><lpage>1128</lpage><history><date date-type="received"><day>27</day><month>1</month><year>2017</year></date><date date-type="accepted"><day>23</day><month>5</month><year>2017</year></date></history><permissions><copyright-statement>© Springer-Verlag London 2017</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">So far, there has been scarce information about the status of immunoglobulins (Ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. The purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. For this reason, 50 native breed water buffaloes were studied. Forty of these buffaloes showed various symptoms of digestive disorders and were allocated into 4 groups of equal sizes (group 1: uncategorized stomatitis; group 2: acute traumatic reticuloperitonitis [TRR]; group 3: acute rumen impaction; and group 4: undifferentiated enteritis). Ten apparently healthy buffaloes were randomly selected and considered as a control group. RNA was firstly extracted from the whole blood then a reverse transcription kits was used to convert the RNA to cDNA. Real-time PCR was used to measure the expression of mRNAs of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α, IgG, and IgA, while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as an internal reference. The results of real-time PCR revealed a significant (<italic>P</italic> ≤ 0.05) upregulation of the gene expression of IL-1β, IL-6, IL-10, and TNF-α in blood of diseased buffaloes compared with those of controls<bold>.</bold> Animals showing acute TRP had peak values of both IL-6 and IL-10; while those exhibiting enteritis and rumen impaction had the highest values of IL-1β and TNF-α, respectively. The results of qPCR also revealed a significant (<italic>P</italic> ≤ 0.05) downregulation of both IgG and IgA gene expression in blood of all diseased buffaloes compared with controls<bold>.</bold> The lowest values of both genes were recorded in buffaloes showing acute TRP. The results herein suggest that the tested genes could have a pivotal role in the pathophysiologic mechanism of the underlying diseases.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Buffaloes</kwd><kwd>Digestive disorders</kwd><kwd>Gene expression</kwd><kwd>Cytokines</kwd><kwd>Immunoglobulins</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer-Verlag London Ltd. 2017</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Égypte</li></country></list><tree><country name="Égypte"><noRegion><name sortKey="Youssef, Mohamed A" sort="Youssef, Mohamed A" uniqKey="Youssef M" first="Mohamed A." last="Youssef">Mohamed A. Youssef</name></noRegion><name sortKey="El Ashker, Maged R" sort="El Ashker, Maged R" uniqKey="El Ashker M" first="Maged R." last="El-Ashker">Maged R. El-Ashker</name><name sortKey="Ouda, Mohamed F" sort="Ouda, Mohamed F" uniqKey="Ouda M" first="Mohamed F." last="Ouda">Mohamed F. Ouda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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