CovidV2, PascalFrancis, Corpus, bibRecord, 000006

Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle

Identifieur interne : 000006 ( PascalFrancis/Corpus ); précédent : 000005; suivant : 000007

Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle

Auteurs : Masaharu Fukuda ; Kazufumi Kuga ; Ayako Miyazaki ; Tohru Suzuki ; Keito Tasei ; Tsunehiko Aita ; Masaji Mase ; Makoto Sugiyama ; Hiroshi Tsunemitsu

Source :

Archives of virology [ 0304-8608 ] ; 2012.

RBID : Pascal:12-0254670

Descripteurs français

Pascal (Inist)
- Bovin, Réaction chaîne polymérase multiplex, Réaction chaîne polymérase RT, Détection, Diarrhée, Vétérinaire.

English descriptors

KwdEn :
- Bovine, Detection, Diarrhea, Multiplex polymerase chain reaction, Reverse transcription polymerase chain reaction, Veterinary.

Abstract

A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine corona-virus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10², 10°, 10¹, and 10² TCID₅₀/ml, respectively, and that for GBR was 10⁶ copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0304-8608`
A03		`1`		`@0 Arch. virol.`
A05				`@2 157`
A06				`@2 6`
A08	`01`	`1`	`ENG`	`@1 Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle`
A11	`01`	`1`		`@1 FUKUDA (Masaharu)`
A11	`02`	`1`		`@1 KUGA (Kazufumi)`
A11	`03`	`1`		`@1 MIYAZAKI (Ayako)`
A11	`04`	`1`		`@1 SUZUKI (Tohru)`
A11	`05`	`1`		`@1 TASEI (Keito)`
A11	`06`	`1`		`@1 AITA (Tsunehiko)`
A11	`07`	`1`		`@1 MASE (Masaji)`
A11	`08`	`1`		`@1 SUGIYAMA (Makoto)`
A11	`09`	`1`		`@1 TSUNEMITSU (Hiroshi)`
A14	`01`			`@1 Saitama Prefectural Chuo Livestock Hygiene Service Center, 107-1 Besshocho, Kita-ku @2 Saitama, Saitama 3310821 @3 JPN @Z 1 aut.`
A14	`02`			`@1 The United Graduate School of Veterinary Sciences, Gifu University @2 Gifu 5011193 @3 JPN @Z 1 aut. @Z 2 aut. @Z 7 aut. @Z 8 aut. @Z 9 aut.`
A14	`03`			`@1 Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5 @2 Tsukuba, Ibaraki 3050856 @3 JPN @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 7 aut. @Z 9 aut.`
A14	`04`			`@1 Saitama Prefectural Chuo Livestock Hygiene Service Center @2 Saitama 3310821 @3 JPN @Z 5 aut.`
A14	`05`			`@1 Niigata Prefectural Chuo Livestock Hygiene Service Center @2 Nishikan-ku, Niigata 9590423 @3 JPN @Z 6 aut.`
A20				`@1 1063-1069`
A21				`@1 2012`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 6355 @5 354000509382170070`
A44				`@0 0000 @1 © 2012 INIST-CNRS. All rights reserved.`
A45				`@0 47 ref.`
A47	`01`	`1`		`@0 12-0254670`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Archives of virology`
A66	`01`			`@0 AUT`
C01	`01`		`ENG`	@0 A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine corona-virus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10², 10°, 10¹, and 10² TCID₅₀/ml, respectively, and that for GBR was 10⁶ copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.
C02	`01`	`X`		`@0 002A05C10`
C03	`01`	`X`	`FRE`	`@0 Bovin @5 01`
C03	`01`	`X`	`ENG`	`@0 Bovine @5 01`
C03	`01`	`X`	`SPA`	`@0 Bovino @5 01`
C03	`02`	`X`	`FRE`	`@0 Réaction chaîne polymérase multiplex @5 05`
C03	`02`	`X`	`ENG`	`@0 Multiplex polymerase chain reaction @5 05`
C03	`02`	`X`	`SPA`	`@0 Reacción cadena polimerasa multiplex @5 05`
C03	`03`	`X`	`FRE`	`@0 Réaction chaîne polymérase RT @5 06`
C03	`03`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 06`
C03	`03`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 06`
C03	`04`	`X`	`FRE`	`@0 Détection @5 07`
C03	`04`	`X`	`ENG`	`@0 Detection @5 07`
C03	`04`	`X`	`SPA`	`@0 Detección @5 07`
C03	`05`	`X`	`FRE`	`@0 Diarrhée @5 14`
C03	`05`	`X`	`ENG`	`@0 Diarrhea @5 14`
C03	`05`	`X`	`SPA`	`@0 Diarrea @5 14`
C03	`06`	`X`	`FRE`	`@0 Vétérinaire @5 45`
C03	`06`	`X`	`ENG`	`@0 Veterinary @5 45`
C03	`06`	`X`	`SPA`	`@0 Veterinario @5 45`
C07	`01`	`X`	`FRE`	`@0 Artiodactyla @2 NS`
C07	`01`	`X`	`ENG`	`@0 Artiodactyla @2 NS`
C07	`01`	`X`	`SPA`	`@0 Artiodactyla @2 NS`
C07	`02`	`X`	`FRE`	`@0 Ungulata @2 NS`
C07	`02`	`X`	`ENG`	`@0 Ungulata @2 NS`
C07	`02`	`X`	`SPA`	`@0 Ungulata @2 NS`
C07	`03`	`X`	`FRE`	`@0 Mammalia @2 NS`
C07	`03`	`X`	`ENG`	`@0 Mammalia @2 NS`
C07	`03`	`X`	`SPA`	`@0 Mammalia @2 NS`
C07	`04`	`X`	`FRE`	`@0 Vertebrata @2 NS`
C07	`04`	`X`	`ENG`	`@0 Vertebrata @2 NS`
C07	`04`	`X`	`SPA`	`@0 Vertebrata @2 NS`
C07	`05`	`X`	`FRE`	`@0 Pathologie de l'appareil digestif @5 13`
C07	`05`	`X`	`ENG`	`@0 Digestive diseases @5 13`
C07	`05`	`X`	`SPA`	`@0 Aparato digestivo patología @5 13`
C07	`06`	`X`	`FRE`	`@0 Pathologie de l'intestin @5 16`
C07	`06`	`X`	`ENG`	`@0 Intestinal disease @5 16`
C07	`06`	`X`	`SPA`	`@0 Intestino patología @5 16`
N21				`@1 191`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 12-0254670 INIST
ET :	Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle
AU :	FUKUDA (Masaharu); KUGA (Kazufumi); MIYAZAKI (Ayako); SUZUKI (Tohru); TASEI (Keito); AITA (Tsunehiko); MASE (Masaji); SUGIYAMA (Makoto); TSUNEMITSU (Hiroshi)
AF :	Saitama Prefectural Chuo Livestock Hygiene Service Center, 107-1 Besshocho, Kita-ku/Saitama, Saitama 3310821/Japon (1 aut.); The United Graduate School of Veterinary Sciences, Gifu University/Gifu 5011193/Japon (1 aut., 2 aut., 7 aut., 8 aut., 9 aut.); Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5/Tsukuba, Ibaraki 3050856/Japon (2 aut., 3 aut., 4 aut., 7 aut., 9 aut.); Saitama Prefectural Chuo Livestock Hygiene Service Center/Saitama 3310821/Japon (5 aut.); Niigata Prefectural Chuo Livestock Hygiene Service Center/Nishikan-ku, Niigata 9590423/Japon (6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Archives of virology; ISSN 0304-8608; Autriche; Da. 2012; Vol. 157; No. 6; Pp. 1063-1069; Bibl. 47 ref.
LA :	Anglais
EA :	A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine corona-virus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10², 10°, 10¹, and 10² TCID₅₀/ml, respectively, and that for GBR was 10⁶ copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.
CC :	002A05C10
FD :	Bovin; Réaction chaîne polymérase multiplex; Réaction chaîne polymérase RT; Détection; Diarrhée; Vétérinaire
FG :	Artiodactyla; Ungulata; Mammalia; Vertebrata; Pathologie de l'appareil digestif; Pathologie de l'intestin
ED :	Bovine; Multiplex polymerase chain reaction; Reverse transcription polymerase chain reaction; Detection; Diarrhea; Veterinary
EG :	Artiodactyla; Ungulata; Mammalia; Vertebrata; Digestive diseases; Intestinal disease
SD :	Bovino; Reacción cadena polimerasa multiplex; Reacción cadena polimerasa transcripción inversa; Detección; Diarrea; Veterinario
LO :	INIST-6355.354000509382170070
ID :	12-0254670

Links to Exploration step

Pascal:12-0254670

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle</title>
<author><name sortKey="Fukuda, Masaharu" sort="Fukuda, Masaharu" uniqKey="Fukuda M" first="Masaharu" last="Fukuda">Masaharu Fukuda</name>
<affiliation><inist:fA14 i1="01"><s1>Saitama Prefectural Chuo Livestock Hygiene Service Center, 107-1 Besshocho, Kita-ku</s1>
<s2>Saitama, Saitama 3310821</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kuga, Kazufumi" sort="Kuga, Kazufumi" uniqKey="Kuga K" first="Kazufumi" last="Kuga">Kazufumi Kuga</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Miyazaki, Ayako" sort="Miyazaki, Ayako" uniqKey="Miyazaki A" first="Ayako" last="Miyazaki">Ayako Miyazaki</name>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Suzuki, Tohru" sort="Suzuki, Tohru" uniqKey="Suzuki T" first="Tohru" last="Suzuki">Tohru Suzuki</name>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tasei, Keito" sort="Tasei, Keito" uniqKey="Tasei K" first="Keito" last="Tasei">Keito Tasei</name>
<affiliation><inist:fA14 i1="04"><s1>Saitama Prefectural Chuo Livestock Hygiene Service Center</s1>
<s2>Saitama 3310821</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Aita, Tsunehiko" sort="Aita, Tsunehiko" uniqKey="Aita T" first="Tsunehiko" last="Aita">Tsunehiko Aita</name>
<affiliation><inist:fA14 i1="05"><s1>Niigata Prefectural Chuo Livestock Hygiene Service Center</s1>
<s2>Nishikan-ku, Niigata 9590423</s2>
<s3>JPN</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Mase, Masaji" sort="Mase, Masaji" uniqKey="Mase M" first="Masaji" last="Mase">Masaji Mase</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Sugiyama, Makoto" sort="Sugiyama, Makoto" uniqKey="Sugiyama M" first="Makoto" last="Sugiyama">Makoto Sugiyama</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tsunemitsu, Hiroshi" sort="Tsunemitsu, Hiroshi" uniqKey="Tsunemitsu H" first="Hiroshi" last="Tsunemitsu">Hiroshi Tsunemitsu</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">12-0254670</idno>
<date when="2012">2012</date>
<idno type="stanalyst">PASCAL 12-0254670 INIST</idno>
<idno type="RBID">Pascal:12-0254670</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000006</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle</title>
<author><name sortKey="Fukuda, Masaharu" sort="Fukuda, Masaharu" uniqKey="Fukuda M" first="Masaharu" last="Fukuda">Masaharu Fukuda</name>
<affiliation><inist:fA14 i1="01"><s1>Saitama Prefectural Chuo Livestock Hygiene Service Center, 107-1 Besshocho, Kita-ku</s1>
<s2>Saitama, Saitama 3310821</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kuga, Kazufumi" sort="Kuga, Kazufumi" uniqKey="Kuga K" first="Kazufumi" last="Kuga">Kazufumi Kuga</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Miyazaki, Ayako" sort="Miyazaki, Ayako" uniqKey="Miyazaki A" first="Ayako" last="Miyazaki">Ayako Miyazaki</name>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Suzuki, Tohru" sort="Suzuki, Tohru" uniqKey="Suzuki T" first="Tohru" last="Suzuki">Tohru Suzuki</name>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tasei, Keito" sort="Tasei, Keito" uniqKey="Tasei K" first="Keito" last="Tasei">Keito Tasei</name>
<affiliation><inist:fA14 i1="04"><s1>Saitama Prefectural Chuo Livestock Hygiene Service Center</s1>
<s2>Saitama 3310821</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Aita, Tsunehiko" sort="Aita, Tsunehiko" uniqKey="Aita T" first="Tsunehiko" last="Aita">Tsunehiko Aita</name>
<affiliation><inist:fA14 i1="05"><s1>Niigata Prefectural Chuo Livestock Hygiene Service Center</s1>
<s2>Nishikan-ku, Niigata 9590423</s2>
<s3>JPN</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Mase, Masaji" sort="Mase, Masaji" uniqKey="Mase M" first="Masaji" last="Mase">Masaji Mase</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Sugiyama, Makoto" sort="Sugiyama, Makoto" uniqKey="Sugiyama M" first="Makoto" last="Sugiyama">Makoto Sugiyama</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tsunemitsu, Hiroshi" sort="Tsunemitsu, Hiroshi" uniqKey="Tsunemitsu H" first="Hiroshi" last="Tsunemitsu">Hiroshi Tsunemitsu</name>
<affiliation><inist:fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bovine</term>
<term>Detection</term>
<term>Diarrhea</term>
<term>Multiplex polymerase chain reaction</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>Veterinary</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Bovin</term>
<term>Réaction chaîne polymérase multiplex</term>
<term>Réaction chaîne polymérase RT</term>
<term>Détection</term>
<term>Diarrhée</term>
<term>Vétérinaire</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine corona-virus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10<sup>2</sup>
, 10°, 10<sup>1</sup>
, and 10<sup>2</sup>
 TCID<sub>50</sub>
/ml, respectively, and that for GBR was 10<sup>6</sup>
 copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0304-8608</s0>
</fA01>
<fA03 i2="1"><s0>Arch. virol.</s0>
</fA03>
<fA05><s2>157</s2>
</fA05>
<fA06><s2>6</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>FUKUDA (Masaharu)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>KUGA (Kazufumi)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>MIYAZAKI (Ayako)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>SUZUKI (Tohru)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>TASEI (Keito)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>AITA (Tsunehiko)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>MASE (Masaji)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>SUGIYAMA (Makoto)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>TSUNEMITSU (Hiroshi)</s1>
</fA11>
<fA14 i1="01"><s1>Saitama Prefectural Chuo Livestock Hygiene Service Center, 107-1 Besshocho, Kita-ku</s1>
<s2>Saitama, Saitama 3310821</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>The United Graduate School of Veterinary Sciences, Gifu University</s1>
<s2>Gifu 5011193</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5</s1>
<s2>Tsukuba, Ibaraki 3050856</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Saitama Prefectural Chuo Livestock Hygiene Service Center</s1>
<s2>Saitama 3310821</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Niigata Prefectural Chuo Livestock Hygiene Service Center</s1>
<s2>Nishikan-ku, Niigata 9590423</s2>
<s3>JPN</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA20><s1>1063-1069</s1>
</fA20>
<fA21><s1>2012</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>6355</s2>
<s5>354000509382170070</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2012 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>47 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>12-0254670</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Archives of virology</s0>
</fA64>
<fA66 i1="01"><s0>AUT</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine corona-virus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10<sup>2</sup>
, 10°, 10<sup>1</sup>
, and 10<sup>2</sup>
 TCID<sub>50</sub>
/ml, respectively, and that for GBR was 10<sup>6</sup>
 copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Bovin</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Bovine</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Bovino</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Réaction chaîne polymérase multiplex</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Multiplex polymerase chain reaction</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Reacción cadena polimerasa multiplex</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Réaction chaîne polymérase RT</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Reverse transcription polymerase chain reaction</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Reacción cadena polimerasa transcripción inversa</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Détection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Detection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Detección</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Diarrhée</s0>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Diarrhea</s0>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Diarrea</s0>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Vétérinaire</s0>
<s5>45</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Veterinary</s0>
<s5>45</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Veterinario</s0>
<s5>45</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Artiodactyla</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Artiodactyla</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Artiodactyla</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Ungulata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Ungulata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Ungulata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Pathologie de l'appareil digestif</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Digestive diseases</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Aparato digestivo patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Pathologie de l'intestin</s0>
<s5>16</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Intestinal disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Intestino patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>191</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 12-0254670 INIST</NO>
<ET>Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle</ET>
<AU>FUKUDA (Masaharu); KUGA (Kazufumi); MIYAZAKI (Ayako); SUZUKI (Tohru); TASEI (Keito); AITA (Tsunehiko); MASE (Masaji); SUGIYAMA (Makoto); TSUNEMITSU (Hiroshi)</AU>
<AF>Saitama Prefectural Chuo Livestock Hygiene Service Center, 107-1 Besshocho, Kita-ku/Saitama, Saitama 3310821/Japon (1 aut.); The United Graduate School of Veterinary Sciences, Gifu University/Gifu 5011193/Japon (1 aut., 2 aut., 7 aut., 8 aut., 9 aut.); Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Kannondai 3-1-5/Tsukuba, Ibaraki 3050856/Japon (2 aut., 3 aut., 4 aut., 7 aut., 9 aut.); Saitama Prefectural Chuo Livestock Hygiene Service Center/Saitama 3310821/Japon (5 aut.); Niigata Prefectural Chuo Livestock Hygiene Service Center/Nishikan-ku, Niigata 9590423/Japon (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Archives of virology; ISSN 0304-8608; Autriche; Da. 2012; Vol. 157; No. 6; Pp. 1063-1069; Bibl. 47 ref.</SO>
<LA>Anglais</LA>
<EA>A one-step multiplex reverse transcription (RT)-PCR method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group A rotavirus (GAR), bovine group B rotavirus (GBR), bovine group C rotavirus (GCR), bovine corona-virus (BCV), and bovine torovirus (BToV). The detection limit of the one-step multiplex RT-PCR for GAR, GCR, BCV, and BToV was 10<sup>2</sup>
, 10°, 10<sup>1</sup>
, and 10<sup>2</sup>
 TCID<sub>50</sub>
/ml, respectively, and that for GBR was 10<sup>6</sup>
 copies/ml. The one-step multiplex RT-PCR with newly designed primers to detect GAR had higher sensitivity than a single RT-PCR with conventional primers, with no false-positive reactions observed for ten other kinds of bovine RNA viruses To assess its field applicability, 59 of 60 fecal samples containing one of these five viruses from all 25 epidemic diarrhea outbreaks in adult cattle were positive in the one-step multiplex RT-PCR assay. Furthermore, using four additional fecal samples containing two viruses (GBR and BCV or BToV), two amplified products of the expected sizes were obtained simultaneously. In contrast, all 80 fecal samples lacking the five target viruses from normal adult cattle were negative in the multiplex assay. Taken together, our results indicate that the one-step multiplex RT-PCR developed here for the detection of GAR, GBR, GCR, BCV, and BToV can be expected to be a useful tool for the rapid and cost-effective diagnosis and surveillance of viral diarrhea in adult cattle.</EA>
<CC>002A05C10</CC>
<FD>Bovin; Réaction chaîne polymérase multiplex; Réaction chaîne polymérase RT; Détection; Diarrhée; Vétérinaire</FD>
<FG>Artiodactyla; Ungulata; Mammalia; Vertebrata; Pathologie de l'appareil digestif; Pathologie de l'intestin</FG>
<ED>Bovine; Multiplex polymerase chain reaction; Reverse transcription polymerase chain reaction; Detection; Diarrhea; Veterinary</ED>
<EG>Artiodactyla; Ungulata; Mammalia; Vertebrata; Digestive diseases; Intestinal disease</EG>
<SD>Bovino; Reacción cadena polimerasa multiplex; Reacción cadena polimerasa transcripción inversa; Detección; Diarrea; Veterinario</SD>
<LO>INIST-6355.354000509382170070</LO>
<ID>12-0254670</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/PascalFrancis/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000006 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000006 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    CovidV2
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:12-0254670
   |texte=   Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle
}}

This area was generated with Dilib version V0.6.33.
Data generation: Sat Mar 28 17:51:24 2020. Site generation: Sun Jan 31 15:35:48 2021

	Serveur d'exploration Covid (26 mars)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Covid (26 mars)

Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle

Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle

Source :

Descripteurs français

English descriptors

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri