Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells
Identifieur interne : 001627 ( Ncbi/Curation ); précédent : 001626; suivant : 001628Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells
Auteurs : Evelyn Albu ; Kathryn V. HolmesSource :
- Journal of Virology [ 0022-538X ] ; 1973.
Abstract
An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70
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PubMed: 4797266
PubMed Central: 356749
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<author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V." last="Holmes">Kathryn V. Holmes</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells</title>
<author><name sortKey="Albu, Evelyn" sort="Albu, Evelyn" uniqKey="Albu E" first="Evelyn" last="Albu">Evelyn Albu</name>
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<author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V." last="Holmes">Kathryn V. Holmes</name>
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<series><title level="j">Journal of Virology</title>
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<front><div type="abstract" xml:lang="en"><p>An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70<italic>S</italic>
in sucrose density gradients and has a molecular weight of approximately 10<sup>7</sup>
, as estimated by gel electrophoresis. The R-type VLP can be labeled with <sup>3</sup>
H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.</p>
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