Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells
Identifieur interne : 000475 ( Pmc/Corpus ); précédent : 000474; suivant : 000476Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells
Auteurs : Evelyn Albu ; Kathryn V. HolmesSource :
- Journal of Virology [ 0022-538X ] ; 1973.
Abstract
An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70
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PubMed: 4797266
PubMed Central: 356749
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells</title><author><name sortKey="Albu, Evelyn" sort="Albu, Evelyn" uniqKey="Albu E" first="Evelyn" last="Albu">Evelyn Albu</name></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V." last="Holmes">Kathryn V. Holmes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">4797266</idno><idno type="pmc">356749</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC356749</idno><idno type="RBID">PMC:356749</idno><date when="1973">1973</date><idno type="wicri:Area/Pmc/Corpus">000475</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000475</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells</title><author><name sortKey="Albu, Evelyn" sort="Albu, Evelyn" uniqKey="Albu E" first="Evelyn" last="Albu">Evelyn Albu</name></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V." last="Holmes">Kathryn V. Holmes</name></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="1973">1973</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70<italic>S</italic> in sucrose density gradients and has a molecular weight of approximately 10<sup>7</sup>, as estimated by gel electrophoresis. The R-type VLP can be labeled with <sup>3</sup>H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jvirol00263-0230-a" xlink:role="1166"></graphic></fig><fig id="F2"><graphic xlink:href="jvirol00263-0230-b" xlink:role="1166"></graphic></fig><fig id="F3"><graphic xlink:href="jvirol00263-0232-a" xlink:role="1168"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn></journal-meta><article-meta><article-id pub-id-type="pmid">4797266</article-id><article-id pub-id-type="pmc">356749</article-id><article-categories><subj-group subj-group-type="heading"><subject>Animal Viruses</subject></subj-group></article-categories><title-group><article-title>Isolation and Preliminary Characterization of the RNA-Containing R-Type, Virus-Like Particle of BHK-21 Cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Albu</surname><given-names>Evelyn</given-names></name><xref ref-type="author-notes" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Holmes</surname><given-names>Kathryn V.</given-names></name><xref ref-type="author-notes" rid="au2">2</xref></contrib></contrib-group><aff id="af1"><label>a</label>Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D.C. 20007</aff><author-notes><fn id="au1"><label>1</label><p> Present address: Schering Corporation, Galloping Hill Rd., Kenilworth, N.J. 07033.</p></fn><fn id="au2"><label>2</label><p> Present address: Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Tex. 75235.</p></fn></author-notes><pub-date pub-type="ppub"><month>11</month><year>1973</year></pub-date><volume>12</volume><issue>5</issue><fpage>1164</fpage><lpage>1172</lpage><copyright-statement>Copyright © 1973 American Society for Microbiology</copyright-statement><abstract><p>An R-type virus-like particle (VLP) has been isolated from the medium of BHK-21-F cells by ultracentrifugation and polyethylene glycol precipitation. The R-type VLP contains RNA which sediments at 60 to 70<italic>S</italic> in sucrose density gradients and has a molecular weight of approximately 10<sup>7</sup>, as estimated by gel electrophoresis. The R-type VLP can be labeled with <sup>3</sup>H-uridine in the presence of actinomycin D. On the basis of morphology, site of maturation, and preliminary biochemical characterization, the R-type VLP does not appear to fit into any of the major groups of animal viruses.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jvirol00263-0230-a" xlink:role="1166"></graphic></fig><fig id="F2"><graphic xlink:href="jvirol00263-0230-b" xlink:role="1166"></graphic></fig><fig id="F3"><graphic xlink:href="jvirol00263-0232-a" xlink:role="1168"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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