Identification of an infectious bronchitis coronavirus strain exhibiting a classical genotype but altered antigenicity, pathogenicity, and innate immunity profile
Identifieur interne : 000659 ( Ncbi/Curation ); précédent : 000658; suivant : 000660Identification of an infectious bronchitis coronavirus strain exhibiting a classical genotype but altered antigenicity, pathogenicity, and innate immunity profile
Auteurs : Shu-Yi Lin [Taïwan] ; Yao-Tsun Li [Taïwan] ; You-Ting Chen [Taïwan] ; Ting-Chih Chen [Taïwan] ; Che-Ming J. Hu [Taïwan] ; Hui-Wen Chen [Taïwan]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
Avian coronavirus infectious bronchitis virus (IBV) poses economic threat to the poultry industry worldwide. Pathogenic IBV 3575/08 was isolated from broilers vaccinated with the attenuated viral vaccine derived from a Taiwan strain 2575/98. In this study, extensive investigations were conducted on the genome sequences, antigenicity, pathogenicity, and host immune responses of several IBV strains in specific-pathogen-free chickens. Sequence analyses revealed that 3575/08 and 2575/98 shared high homology in their structural genes, but not in non-structural accessory proteins such as 3a, 3b and 5b. Despite a high degree of homology in their spike protein genes, cross neutralization test showed low cross protection between 3575/08 and 2575/98, suggesting distinct antigenicity for the two strains. Animal challenge experiments exhibited strong respiratory and renal pathogenicity for 3575/08. In addition, early and prolonged viral shedding and rapid viral dissemination were observed. Immune gene expression profiling by PCR array showed chickens infected with 3575/08 had delayed expression of a subset of early innate immune genes, whereas chickens infected with the wild-type or attenuated-type 2575/08 revealed quick gene induction and efficient virus control. In summary, this study reveals a new IBV strain, which harbors a known local genotype but displays remarkably altered antigenicity, pathogenicity and host defenses.
The online version of this article (doi:10.1038/srep37725) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1038/srep37725
PubMed: 27876864
PubMed Central: 5120290
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Pathogenic IBV 3575/08 was isolated from broilers vaccinated with the attenuated viral vaccine derived from a Taiwan strain 2575/98. In this study, extensive investigations were conducted on the genome sequences, antigenicity, pathogenicity, and host immune responses of several IBV strains in specific-pathogen-free chickens. Sequence analyses revealed that 3575/08 and 2575/98 shared high homology in their structural genes, but not in non-structural accessory proteins such as 3a, 3b and 5b. Despite a high degree of homology in their spike protein genes, cross neutralization test showed low cross protection between 3575/08 and 2575/98, suggesting distinct antigenicity for the two strains. Animal challenge experiments exhibited strong respiratory and renal pathogenicity for 3575/08. In addition, early and prolonged viral shedding and rapid viral dissemination were observed. Immune gene expression profiling by PCR array showed chickens infected with 3575/08 had delayed expression of a subset of early innate immune genes, whereas chickens infected with the wild-type or attenuated-type 2575/08 revealed quick gene induction and efficient virus control. In summary, this study reveals a new IBV strain, which harbors a known local genotype but displays remarkably altered antigenicity, pathogenicity and host defenses.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1038/srep37725) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cook, Jk" uniqKey="Cook J">JK Cook</name></author><author><name sortKey="Jackwood, M" uniqKey="Jackwood M">M Jackwood</name></author><author><name sortKey="Jones, Rc" uniqKey="Jones R">RC Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jackwood, Mw" uniqKey="Jackwood M">MW Jackwood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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Muench</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name></author><author><name sortKey="Dudley, J" uniqKey="Dudley J">J Dudley</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Archetti, I" uniqKey="Archetti I">I Archetti</name></author><author><name sortKey="Horsfall, Fl" uniqKey="Horsfall F">FL Horsfall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Choi, Ks" uniqKey="Choi K">KS Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Avellaneda, Ge" uniqKey="Avellaneda G">GE Avellaneda</name></author><author><name sortKey="Villegas, P" uniqKey="Villegas P">P Villegas</name></author><author><name sortKey="Jackwood, Mw" uniqKey="Jackwood M">MW Jackwood</name></author><author><name sortKey="King, Dj" uniqKey="King D">DJ King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapczynski, Dr" uniqKey="Kapczynski D">DR Kapczynski</name></author><author><name sortKey="Jiang, Hj" uniqKey="Jiang H">HJ Jiang</name></author><author><name sortKey="Kogut, Mh" uniqKey="Kogut M">MH Kogut</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Livak, Kj" uniqKey="Livak K">KJ Livak</name></author><author><name sortKey="Schmittgen, Td" uniqKey="Schmittgen T">TD Schmittgen</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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