Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
Identifieur interne : 000264 ( Ncbi/Checkpoint ); précédent : 000263; suivant : 000265Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
Auteurs : Jin Yang [République populaire de Chine] ; Mei-Xin Fang [République populaire de Chine] ; Jie Li [République populaire de Chine] ; Guo-Qiang Lou [République populaire de Chine] ; Hang-Jun Lu [République populaire de Chine] ; Nan-Ping Wu [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 2011.
Abstract
An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.
Url:
DOI: 10.1007/s00705-011-1001-4
PubMed: 21562883
PubMed Central: 7087303
Affiliations:
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id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author></analytic><series><title level="j">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Aoi, Y" uniqKey="Aoi Y">Y Aoi</name></author><author><name sortKey="Hosogai, M" uniqKey="Hosogai M">M Hosogai</name></author><author><name sortKey="Tsuneda, S" uniqKey="Tsuneda S">S Tsuneda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ascione, A" uniqKey="Ascione A">A Ascione</name></author><author><name sortKey="Tartaglione, T" uniqKey="Tartaglione T">T Tartaglione</name></author><author><name sortKey="Di Costanzo, Gg" uniqKey="Di Costanzo G">GG Di Costanzo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bashiardes, S" uniqKey="Bashiardes S">S Bashiardes</name></author><author><name sortKey="Richter, J" 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Sizmann</name></author><author><name sortKey="Babiel, R" uniqKey="Babiel R">R Babiel</name></author><author><name sortKey="Mihm, U" uniqKey="Mihm U">U Mihm</name></author><author><name sortKey="Hofmann, Wp" uniqKey="Hofmann W">WP Hofmann</name></author><author><name sortKey="Von Wagner, M" uniqKey="Von Wagner M">M von Wagner</name></author><author><name sortKey="Zeuzem, S" uniqKey="Zeuzem S">S Zeuzem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tomita, N" uniqKey="Tomita N">N Tomita</name></author><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name></author><author><name sortKey="Kanda, H" uniqKey="Kanda H">H Kanda</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tramarin, A" uniqKey="Tramarin A">A Tramarin</name></author><author><name sortKey="Gennaro, N" uniqKey="Gennaro N">N Gennaro</name></author><author><name sortKey="Compostella, Fa" uniqKey="Compostella F">FA Compostella</name></author><author><name sortKey="Gallo, C" uniqKey="Gallo C">C Gallo</name></author><author><name sortKey="Wendelaar Bonga, Lj" uniqKey="Wendelaar Bonga L">LJ Wendelaar Bonga</name></author><author><name sortKey="Postma, Mj" uniqKey="Postma M">MJ Postma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wei, Y" uniqKey="Wei Y">Y Wei</name></author><author><name sortKey="Yang, C" uniqKey="Yang C">C Yang</name></author><author><name sortKey="Wei, B" uniqKey="Wei B">B Wei</name></author><author><name sortKey="Huang, J" uniqKey="Huang J">J Huang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Meng, S" uniqKey="Meng S">S Meng</name></author><author><name sortKey="Zhang, R" uniqKey="Zhang R">R Zhang</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Sx" uniqKey="Xu S">SX Xu</name></author><author><name sortKey="Lan, K" uniqKey="Lan K">K Lan</name></author><author><name sortKey="Shan, Yl" uniqKey="Shan Y">YL Shan</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Luo, Jy" uniqKey="Luo J">JY Luo</name></author><author><name sortKey="Cui, F" uniqKey="Cui F">F Cui</name></author><author><name sortKey="Huang, Ys" uniqKey="Huang Y">YS Huang</name></author><author><name sortKey="Meng, Jp" uniqKey="Meng J">JP Meng</name></author><author><name sortKey="Zhang, Xm" uniqKey="Zhang X">XM Zhang</name></author><author><name sortKey="Yin, Yb" uniqKey="Yin Y">YB Yin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoshida, N" uniqKey="Yoshida N">N Yoshida</name></author><author><name sortKey="Fujino, M" uniqKey="Fujino M">M Fujino</name></author><author><name sortKey="Miyata, A" uniqKey="Miyata A">A Miyata</name></author><author><name sortKey="Nagai, T" uniqKey="Nagai T">T Nagai</name></author><author><name sortKey="Kamada, M" uniqKey="Kamada M">M Kamada</name></author><author><name sortKey="Sakiyama, H" uniqKey="Sakiyama H">H Sakiyama</name></author><author><name sortKey="Ihara, T" uniqKey="Ihara T">T Ihara</name></author><author><name sortKey="Kumagai, T" uniqKey="Kumagai T">T Kumagai</name></author><author><name sortKey="Okafuji, T" uniqKey="Okafuji T">T Okafuji</name></author><author><name sortKey="Nakayama, T" uniqKey="Nakayama T">T Nakayama</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yang, Jin" sort="Yang, Jin" uniqKey="Yang J" first="Jin" last="Yang">Jin Yang</name></noRegion><name sortKey="Fang, Mei Xin" sort="Fang, Mei Xin" uniqKey="Fang M" first="Mei-Xin" last="Fang">Mei-Xin Fang</name><name sortKey="Li, Jie" sort="Li, Jie" uniqKey="Li J" first="Jie" last="Li">Jie Li</name><name sortKey="Lou, Guo Qiang" sort="Lou, Guo Qiang" uniqKey="Lou G" first="Guo-Qiang" last="Lou">Guo-Qiang Lou</name><name sortKey="Lu, Hang Jun" sort="Lu, Hang Jun" uniqKey="Lu H" first="Hang-Jun" last="Lu">Hang-Jun Lu</name><name sortKey="Wu, Nan Ping" sort="Wu, Nan Ping" uniqKey="Wu N" first="Nan-Ping" last="Wu">Nan-Ping Wu</name><name sortKey="Yang, Jin" sort="Yang, Jin" uniqKey="Yang J" first="Jin" last="Yang">Jin Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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