Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
Identifieur interne : 000175 ( Pmc/Curation ); précédent : 000174; suivant : 000176Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
Auteurs : Jin Yang [République populaire de Chine] ; Mei-Xin Fang [République populaire de Chine] ; Jie Li [République populaire de Chine] ; Guo-Qiang Lou [République populaire de Chine] ; Hang-Jun Lu [République populaire de Chine] ; Nan-Ping Wu [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 2011.
Abstract
An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.
Url:
DOI: 10.1007/s00705-011-1001-4
PubMed: 21562883
PubMed Central: 7087303
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000175
Links to Exploration step
PMC:7087303Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay</title><author><name sortKey="Yang, Jin" sort="Yang, Jin" uniqKey="Yang J" first="Jin" last="Yang">Jin Yang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410621.0</institution-id><institution>Department of Medicine,</institution><institution>Blood Center of Zhejiang Province,</institution></institution-wrap>Hangzhou, 310005 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Fang, Mei Xin" sort="Fang, Mei Xin" uniqKey="Fang M" first="Mei-Xin" last="Fang">Mei-Xin Fang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Li, Jie" sort="Li, Jie" uniqKey="Li J" first="Jie" last="Li">Jie Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Lou, Guo Qiang" sort="Lou, Guo Qiang" uniqKey="Lou G" first="Guo-Qiang" last="Lou">Guo-Qiang Lou</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.460074.1</institution-id><institution>Department of Infectious Diseases, School of Medicine,</institution><institution>The Affiliated Hospital of Hangzhou Normal University,</institution></institution-wrap>Hangzhou, 310018 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Hang Jun" sort="Lu, Hang Jun" uniqKey="Lu H" first="Hang-Jun" last="Lu">Hang-Jun Lu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410621.0</institution-id><institution>Department of Medicine,</institution><institution>Blood Center of Zhejiang Province,</institution></institution-wrap>Hangzhou, 310005 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Nan Ping" sort="Wu, Nan Ping" uniqKey="Wu N" first="Nan-Ping" last="Wu">Nan-Ping Wu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21562883</idno><idno type="pmc">7087303</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087303</idno><idno type="RBID">PMC:7087303</idno><idno type="doi">10.1007/s00705-011-1001-4</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000175</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000175</idno><idno type="wicri:Area/Pmc/Curation">000175</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000175</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay</title><author><name sortKey="Yang, Jin" sort="Yang, Jin" uniqKey="Yang J" first="Jin" last="Yang">Jin Yang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410621.0</institution-id><institution>Department of Medicine,</institution><institution>Blood Center of Zhejiang Province,</institution></institution-wrap>Hangzhou, 310005 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Fang, Mei Xin" sort="Fang, Mei Xin" uniqKey="Fang M" first="Mei-Xin" last="Fang">Mei-Xin Fang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Li, Jie" sort="Li, Jie" uniqKey="Li J" first="Jie" last="Li">Jie Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Lou, Guo Qiang" sort="Lou, Guo Qiang" uniqKey="Lou G" first="Guo-Qiang" last="Lou">Guo-Qiang Lou</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.460074.1</institution-id><institution>Department of Infectious Diseases, School of Medicine,</institution><institution>The Affiliated Hospital of Hangzhou Normal University,</institution></institution-wrap>Hangzhou, 310018 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Hang Jun" sort="Lu, Hang Jun" uniqKey="Lu H" first="Hang-Jun" last="Lu">Hang-Jun Lu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410621.0</institution-id><institution>Department of Medicine,</institution><institution>Blood Center of Zhejiang Province,</institution></institution-wrap>Hangzhou, 310005 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Nan Ping" sort="Wu, Nan Ping" uniqKey="Wu N" first="Nan-Ping" last="Wu">Nan-Ping Wu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hangzhou</wicri:regionArea></affiliation></author></analytic><series><title level="j">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Aoi, Y" uniqKey="Aoi Y">Y Aoi</name></author><author><name sortKey="Hosogai, M" uniqKey="Hosogai M">M Hosogai</name></author><author><name sortKey="Tsuneda, S" uniqKey="Tsuneda S">S Tsuneda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ascione, A" uniqKey="Ascione A">A Ascione</name></author><author><name sortKey="Tartaglione, T" uniqKey="Tartaglione T">T Tartaglione</name></author><author><name sortKey="Di Costanzo, Gg" uniqKey="Di Costanzo G">GG Di Costanzo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bashiardes, S" uniqKey="Bashiardes S">S Bashiardes</name></author><author><name sortKey="Richter, J" uniqKey="Richter J">J Richter</name></author><author><name sortKey="Christodoulou, Cg" uniqKey="Christodoulou C">CG Christodoulou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Castelain, S" uniqKey="Castelain S">S Castelain</name></author><author><name sortKey="Descamps, V" uniqKey="Descamps V">V Descamps</name></author><author><name sortKey="Thibault, V" uniqKey="Thibault V">V Thibault</name></author><author><name sortKey="Francois, C" uniqKey="Francois C">C Francois</name></author><author><name sortKey="Bonte, D" uniqKey="Bonte D">D Bonte</name></author><author><name sortKey="Morel, V" uniqKey="Morel V">V Morel</name></author><author><name sortKey="Izopet, J" uniqKey="Izopet J">J Izopet</name></author><author><name sortKey="Capron, D" uniqKey="Capron D">D Capron</name></author><author><name sortKey="Zawadzki, P" uniqKey="Zawadzki P">P Zawadzki</name></author><author><name sortKey="Duverlie, G" uniqKey="Duverlie G">G Duverlie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Crignis, Ed" uniqKey="Crignis E">ED Crignis</name></author><author><name sortKey="Re, Mc" uniqKey="Re M">MC Re</name></author><author><name sortKey="Cimatti, L" uniqKey="Cimatti L">L Cimatti</name></author><author><name sortKey="Zecchi, L" uniqKey="Zecchi L">L Zecchi</name></author><author><name sortKey="Gibellini, D" uniqKey="Gibellini D">D Gibellini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Curtis, Ka" uniqKey="Curtis K">KA Curtis</name></author><author><name sortKey="Rudolph, Dl" uniqKey="Rudolph D">DL Rudolph</name></author><author><name sortKey="Owen, Sm" uniqKey="Owen S">SM Owen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Daniel, Hd" uniqKey="Daniel H">HD Daniel</name></author><author><name sortKey="Grant, Pr" uniqKey="Grant P">PR Grant</name></author><author><name sortKey="Garson, Ja" uniqKey="Garson J">JA Garson</name></author><author><name sortKey="Tedder, Rs" uniqKey="Tedder R">RS Tedder</name></author><author><name sortKey="Chandy, Gm" uniqKey="Chandy G">GM Chandy</name></author><author><name sortKey="Abraham, P" uniqKey="Abraham P">P Abraham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Kupfer, B" uniqKey="Kupfer B">B Kupfer</name></author><author><name sortKey="Petersen, N" uniqKey="Petersen N">N Petersen</name></author><author><name sortKey="Grotto, Rm" uniqKey="Grotto R">RM Grotto</name></author><author><name sortKey="Rodrigues, Sm" uniqKey="Rodrigues S">SM Rodrigues</name></author><author><name sortKey="Grywna, K" uniqKey="Grywna K">K Grywna</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Annan, A" uniqKey="Annan A">A Annan</name></author><author><name sortKey="Silva, Gf" uniqKey="Silva G">GF Silva</name></author><author><name sortKey="Douglas, J" uniqKey="Douglas J">J Douglas</name></author><author><name sortKey="Koay, Es" uniqKey="Koay E">ES Koay</name></author><author><name sortKey="Smuts, H" uniqKey="Smuts H">H Smuts</name></author><author><name sortKey="Netto, Em" uniqKey="Netto E">EM Netto</name></author><author><name sortKey="Simmonds, P" uniqKey="Simmonds P">P Simmonds</name></author><author><name sortKey="Pardini, Mi" uniqKey="Pardini M">MI Pardini</name></author><author><name sortKey="Roth, Wk" uniqKey="Roth W">WK Roth</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fang, X" uniqKey="Fang X">X Fang</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Kong, J" uniqKey="Kong J">J Kong</name></author><author><name sortKey="Jiang, X" uniqKey="Jiang X">X Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glynn, Sa" uniqKey="Glynn S">SA Glynn</name></author><author><name sortKey="Wright, Dj" uniqKey="Wright D">DJ Wright</name></author><author><name sortKey="Kleinman, Sh" uniqKey="Kleinman S">SH Kleinman</name></author><author><name sortKey="Hirschkorn, D" uniqKey="Hirschkorn D">D Hirschkorn</name></author><author><name sortKey="Tu, Y" uniqKey="Tu Y">Y Tu</name></author><author><name sortKey="Heldebrant, C" uniqKey="Heldebrant C">C Heldebrant</name></author><author><name sortKey="Smith, R" uniqKey="Smith R">R Smith</name></author><author><name sortKey="Giachetti, C" uniqKey="Giachetti C">C Giachetti</name></author><author><name sortKey="Gallarda, J" uniqKey="Gallarda J">J Gallarda</name></author><author><name sortKey="Busch, Mp" uniqKey="Busch M">MP Busch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gonzalez Perez, I" uniqKey="Gonzalez Perez I">I Gonzalez-Perez</name></author><author><name sortKey="Vina Rodriguez, A" uniqKey="Vina Rodriguez A">A Vina-Rodriguez</name></author><author><name sortKey="Cayarga, Aa" uniqKey="Cayarga A">AA Cayarga</name></author><author><name sortKey="Rosa, Ig" uniqKey="Rosa I">IG Rosa</name></author><author><name sortKey="Gonzalez, Yj" uniqKey="Gonzalez Y">YJ Gonzalez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guichon, A" uniqKey="Guichon A">A Guichon</name></author><author><name sortKey="Chiparelli, H" uniqKey="Chiparelli H">H Chiparelli</name></author><author><name sortKey="Martinez, A" uniqKey="Martinez A">A Martinez</name></author><author><name sortKey="Rodriguez, C" uniqKey="Rodriguez C">C Rodriguez</name></author><author><name sortKey="Trento, A" uniqKey="Trento A">A Trento</name></author><author><name sortKey="Russi, Jc" uniqKey="Russi J">JC Russi</name></author><author><name sortKey="Carballal, G" uniqKey="Carballal G">G Carballal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kleter, Ge" uniqKey="Kleter G">GE Kleter</name></author><author><name sortKey="Van Doorn, Lj" uniqKey="Van Doorn L">LJ van Doorn</name></author><author><name sortKey="Brouwer, Jt" uniqKey="Brouwer J">JT Brouwer</name></author><author><name sortKey="Schalm, Sw" uniqKey="Schalm S">SW Schalm</name></author><author><name sortKey="Heijtink, Ra" uniqKey="Heijtink R">RA Heijtink</name></author><author><name sortKey="Quint, Wg" uniqKey="Quint W">WG Quint</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lan, X" uniqKey="Lan X">X Lan</name></author><author><name sortKey="Yang, B" uniqKey="Yang B">B Yang</name></author><author><name sortKey="Li, By" uniqKey="Li B">BY Li</name></author><author><name sortKey="Yin, Xp" uniqKey="Yin X">XP Yin</name></author><author><name sortKey="Li, Xr" uniqKey="Li X">XR Li</name></author><author><name sortKey="Liu, Jx" uniqKey="Liu J">JX Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Le Guillou Guillemette, H" uniqKey="Le Guillou Guillemette H">H Le Guillou-Guillemette</name></author><author><name sortKey="Lunel Fabiani, F" uniqKey="Lunel Fabiani F">F Lunel-Fabiani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, L" uniqKey="Lu L">L Lu</name></author><author><name sortKey="Nakano, T" uniqKey="Nakano T">T Nakano</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y Fu</name></author><author><name sortKey="Hagedorn, Ch" uniqKey="Hagedorn C">CH Hagedorn</name></author><author><name sortKey="Robertson, Bh" uniqKey="Robertson B">BH Robertson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meng, S" uniqKey="Meng S">S Meng</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murphy, Dg" uniqKey="Murphy D">DG Murphy</name></author><author><name sortKey="Willems, B" uniqKey="Willems B">B Willems</name></author><author><name sortKey="Deschenes, M" uniqKey="Deschenes M">M Deschenes</name></author><author><name sortKey="Hilzenrat, N" uniqKey="Hilzenrat N">N Hilzenrat</name></author><author><name sortKey="Mousseau, R" uniqKey="Mousseau R">R Mousseau</name></author><author><name sortKey="Sabbah, S" uniqKey="Sabbah S">S Sabbah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name></author><author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author><author><name sortKey="Okayama, H" uniqKey="Okayama H">H Okayama</name></author><author><name sortKey="Masubuchi, H" uniqKey="Masubuchi H">H Masubuchi</name></author><author><name sortKey="Yonekawa, T" uniqKey="Yonekawa T">T Yonekawa</name></author><author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name></author><author><name sortKey="Amino, N" uniqKey="Amino N">N Amino</name></author><author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ozaras, R" uniqKey="Ozaras R">R Ozaras</name></author><author><name sortKey="Tahan, V" uniqKey="Tahan V">V Tahan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parida, M" uniqKey="Parida M">M Parida</name></author><author><name sortKey="Posadas, G" uniqKey="Posadas G">G Posadas</name></author><author><name sortKey="Inoue, S" uniqKey="Inoue S">S Inoue</name></author><author><name sortKey="Hasebe, F" uniqKey="Hasebe F">F Hasebe</name></author><author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parida, M" uniqKey="Parida M">M Parida</name></author><author><name sortKey="Sannarangaiah, S" uniqKey="Sannarangaiah S">S Sannarangaiah</name></author><author><name sortKey="Dash, Pk" uniqKey="Dash P">PK Dash</name></author><author><name sortKey="Rao, Pv" uniqKey="Rao P">PV Rao</name></author><author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sarrazin, C" uniqKey="Sarrazin C">C Sarrazin</name></author><author><name sortKey="Gartner, Bc" uniqKey="Gartner B">BC Gartner</name></author><author><name sortKey="Sizmann, D" uniqKey="Sizmann D">D Sizmann</name></author><author><name sortKey="Babiel, R" uniqKey="Babiel R">R Babiel</name></author><author><name sortKey="Mihm, U" uniqKey="Mihm U">U Mihm</name></author><author><name sortKey="Hofmann, Wp" uniqKey="Hofmann W">WP Hofmann</name></author><author><name sortKey="Von Wagner, M" uniqKey="Von Wagner M">M von Wagner</name></author><author><name sortKey="Zeuzem, S" uniqKey="Zeuzem S">S Zeuzem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tomita, N" uniqKey="Tomita N">N Tomita</name></author><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name></author><author><name sortKey="Kanda, H" uniqKey="Kanda H">H Kanda</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tramarin, A" uniqKey="Tramarin A">A Tramarin</name></author><author><name sortKey="Gennaro, N" uniqKey="Gennaro N">N Gennaro</name></author><author><name sortKey="Compostella, Fa" uniqKey="Compostella F">FA Compostella</name></author><author><name sortKey="Gallo, C" uniqKey="Gallo C">C Gallo</name></author><author><name sortKey="Wendelaar Bonga, Lj" uniqKey="Wendelaar Bonga L">LJ Wendelaar Bonga</name></author><author><name sortKey="Postma, Mj" uniqKey="Postma M">MJ Postma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wei, Y" uniqKey="Wei Y">Y Wei</name></author><author><name sortKey="Yang, C" uniqKey="Yang C">C Yang</name></author><author><name sortKey="Wei, B" uniqKey="Wei B">B Wei</name></author><author><name sortKey="Huang, J" uniqKey="Huang J">J Huang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Meng, S" uniqKey="Meng S">S Meng</name></author><author><name sortKey="Zhang, R" uniqKey="Zhang R">R Zhang</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Sx" uniqKey="Xu S">SX Xu</name></author><author><name sortKey="Lan, K" uniqKey="Lan K">K Lan</name></author><author><name sortKey="Shan, Yl" uniqKey="Shan Y">YL Shan</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Luo, Jy" uniqKey="Luo J">JY Luo</name></author><author><name sortKey="Cui, F" uniqKey="Cui F">F Cui</name></author><author><name sortKey="Huang, Ys" uniqKey="Huang Y">YS Huang</name></author><author><name sortKey="Meng, Jp" uniqKey="Meng J">JP Meng</name></author><author><name sortKey="Zhang, Xm" uniqKey="Zhang X">XM Zhang</name></author><author><name sortKey="Yin, Yb" uniqKey="Yin Y">YB Yin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoshida, N" uniqKey="Yoshida N">N Yoshida</name></author><author><name sortKey="Fujino, M" uniqKey="Fujino M">M Fujino</name></author><author><name sortKey="Miyata, A" uniqKey="Miyata A">A Miyata</name></author><author><name sortKey="Nagai, T" uniqKey="Nagai T">T Nagai</name></author><author><name sortKey="Kamada, M" uniqKey="Kamada M">M Kamada</name></author><author><name sortKey="Sakiyama, H" uniqKey="Sakiyama H">H Sakiyama</name></author><author><name sortKey="Ihara, T" uniqKey="Ihara T">T Ihara</name></author><author><name sortKey="Kumagai, T" uniqKey="Kumagai T">T Kumagai</name></author><author><name sortKey="Okafuji, T" uniqKey="Okafuji T">T Okafuji</name></author><author><name sortKey="Nakayama, T" uniqKey="Nakayama T">T Nakayama</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Arch Virol</journal-id><journal-id journal-id-type="iso-abbrev">Arch. Virol</journal-id><journal-title-group><journal-title>Archives of Virology</journal-title></journal-title-group><issn pub-type="ppub">0304-8608</issn><issn pub-type="epub">1432-8798</issn><publisher><publisher-name>Springer Vienna</publisher-name><publisher-loc>Vienna</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21562883</article-id><article-id pub-id-type="pmc">7087303</article-id><article-id pub-id-type="publisher-id">1001</article-id><article-id pub-id-type="doi">10.1007/s00705-011-1001-4</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Jin</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Fang</surname><given-names>Mei-xin</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Jie</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lou</surname><given-names>Guo-qiang</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Hang-jun</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Wu</surname><given-names>Nan-ping</given-names></name><address><email>flwnp@yahoo.com.cn</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.13402.34</institution-id><institution-id institution-id-type="ISNI">000000041759700X</institution-id><institution>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases,</institution><institution>The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University,</institution></institution-wrap>Hangzhou, 310022 China</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.410621.0</institution-id><institution>Department of Medicine,</institution><institution>Blood Center of Zhejiang Province,</institution></institution-wrap>Hangzhou, 310005 China</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="GRID">grid.460074.1</institution-id><institution>Department of Infectious Diseases, School of Medicine,</institution><institution>The Affiliated Hospital of Hangzhou Normal University,</institution></institution-wrap>Hangzhou, 310018 China</aff></contrib-group><pub-date pub-type="epub"><day>12</day><month>5</month><year>2011</year></pub-date><pub-date pub-type="ppub"><year>2011</year></pub-date><volume>156</volume><issue>8</issue><fpage>1387</fpage><lpage>1396</lpage><history><date date-type="received"><day>27</day><month>12</month><year>2010</year></date><date date-type="accepted"><day>9</day><month>4</month><year>2011</year></date></history><permissions><copyright-statement>© Springer-Verlag 2011</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>West Nile Virus</kwd><kwd>Lamp Assay</kwd><kwd>Lamp Reaction</kwd><kwd>Loop Primer</kwd><kwd>Lamp Method</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer-Verlag 2011</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000175 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000175 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:7087303
|texte= Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:21562883" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |