Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses
Identifieur interne : 001A90 ( Main/Merge ); précédent : 001A89; suivant : 001A91Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses
Auteurs : X. Zhang [États-Unis] ; W. Herbst [Allemagne] ; K. G. Kousoulas [États-Unis] ; J. Storz [États-Unis]Source :
- Archives of Virology [ 0304-8608 ] ; 1994-09-01.
English descriptors
- Teeft :
- Acetylesterase, Acetylesterase activities, Acid changes, Amino, Amino acids, Antigenic properties, Avirulent strains, Becv, Biological properties, Bovine, Bovine coronavirus, Bovine coronaviruses, Brcv, Cell cultures, Cell fusion, Coronavirus, Coronaviruses, Cytopathic, Cytopathic changes, Deletion, Denatured, Erythrocyte, Gastroenteritis, Gastroenteritis virus, Gene sequence, Gene sequences, Glycoprotein, Hemagglutination, Intestinal, Kousoulas, Louisiana state university, Mabs, Monoclonal antibodies, Nasal swabs, Native conditions, Neonatal calf diarrhea, Newborn calves, Nucleotide, Nucleotide changes, Nucleotide sequences, Porcine, Receptor, Respiratory disease, Respiratory tracts, Sequence analysis, Storz, Viral, Virol, Virology, Virus preparation, Western blots, Zhang.
Abstract
Summary: The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.
Url:
- https://api.istex.fr/ark:/67375/1BB-JBRQB606-M/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087011
DOI: 10.1007/BF01310579
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ISTEX:AB13024526B52C301A5F8F691E93608753696F1DLe document en format XML
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Zhang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig Universität Giessen, Giessen</wicri:regionArea><wicri:noRegion>Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. Kousoulas</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Storz, J" sort="Storz, J" uniqKey="Storz J" first="J." last="Storz">J. Storz</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1994-09-01">1994-09-01</date><biblScope unit="volume">134</biblScope><biblScope unit="issue">3-4</biblScope><biblScope unit="page" from="421">421</biblScope><biblScope unit="page" to="426">426</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetylesterase</term><term>Acetylesterase activities</term><term>Acid changes</term><term>Amino</term><term>Amino acids</term><term>Antigenic properties</term><term>Avirulent strains</term><term>Becv</term><term>Biological properties</term><term>Bovine</term><term>Bovine coronavirus</term><term>Bovine coronaviruses</term><term>Brcv</term><term>Cell cultures</term><term>Cell fusion</term><term>Coronavirus</term><term>Coronaviruses</term><term>Cytopathic</term><term>Cytopathic changes</term><term>Deletion</term><term>Denatured</term><term>Erythrocyte</term><term>Gastroenteritis</term><term>Gastroenteritis virus</term><term>Gene sequence</term><term>Gene sequences</term><term>Glycoprotein</term><term>Hemagglutination</term><term>Intestinal</term><term>Kousoulas</term><term>Louisiana state university</term><term>Mabs</term><term>Monoclonal antibodies</term><term>Nasal swabs</term><term>Native conditions</term><term>Neonatal calf diarrhea</term><term>Newborn calves</term><term>Nucleotide</term><term>Nucleotide changes</term><term>Nucleotide sequences</term><term>Porcine</term><term>Receptor</term><term>Respiratory disease</term><term>Respiratory tracts</term><term>Sequence analysis</term><term>Storz</term><term>Viral</term><term>Virol</term><term>Virology</term><term>Virus preparation</term><term>Western blots</term><term>Zhang</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.</div></front></TEI><double doi="10.1007/BF01310579"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses</title><author><name sortKey="Zhang, X" sort="Zhang, X" uniqKey="Zhang X" first="X." last="Zhang">X. Zhang</name></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. Kousoulas</name></author><author><name sortKey="Storz, J" sort="Storz, J" uniqKey="Storz J" first="J." last="Storz">J. Storz</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AB13024526B52C301A5F8F691E93608753696F1D</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1007/BF01310579</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-JBRQB606-M/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000374</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000374</idno><idno type="wicri:Area/Istex/Curation">000341</idno><idno type="wicri:Area/Istex/Checkpoint">000477</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000477</idno><idno type="wicri:doubleKey">0304-8608:1994:Zhang X:comparison:of:the</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses</title><author><name sortKey="Zhang, X" sort="Zhang, X" uniqKey="Zhang X" first="X." last="Zhang">X. Zhang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig Universität Giessen, Giessen</wicri:regionArea><wicri:noRegion>Giessen</wicri:noRegion><wicri:noRegion>Giessen</wicri:noRegion></affiliation></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. Kousoulas</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Storz, J" sort="Storz, J" uniqKey="Storz J" first="J." last="Storz">J. Storz</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1994-09-01">1994-09-01</date><biblScope unit="volume">134</biblScope><biblScope unit="issue">3-4</biblScope><biblScope unit="page" from="421">421</biblScope><biblScope unit="page" to="426">426</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetylesterase</term><term>Acetylesterase activities</term><term>Acid changes</term><term>Amino</term><term>Amino acids</term><term>Antigenic properties</term><term>Avirulent strains</term><term>Becv</term><term>Biological properties</term><term>Bovine</term><term>Bovine coronavirus</term><term>Bovine coronaviruses</term><term>Brcv</term><term>Cell cultures</term><term>Cell fusion</term><term>Coronavirus</term><term>Coronaviruses</term><term>Cytopathic</term><term>Cytopathic changes</term><term>Deletion</term><term>Denatured</term><term>Erythrocyte</term><term>Gastroenteritis</term><term>Gastroenteritis virus</term><term>Gene sequence</term><term>Gene sequences</term><term>Glycoprotein</term><term>Hemagglutination</term><term>Intestinal</term><term>Kousoulas</term><term>Louisiana state university</term><term>Mabs</term><term>Monoclonal antibodies</term><term>Nasal swabs</term><term>Native conditions</term><term>Neonatal calf diarrhea</term><term>Newborn calves</term><term>Nucleotide</term><term>Nucleotide changes</term><term>Nucleotide sequences</term><term>Porcine</term><term>Receptor</term><term>Respiratory disease</term><term>Respiratory tracts</term><term>Sequence analysis</term><term>Storz</term><term>Viral</term><term>Virol</term><term>Virology</term><term>Virus preparation</term><term>Western blots</term><term>Zhang</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.</div></front></TEI></ISTEX><PMC><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses</title><author><name sortKey="Zhang, X" sort="Zhang, X" uniqKey="Zhang X" first="X." last="Zhang">X. Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.8664.c</institution-id><institution-id institution-id-type="ISNI">0000000121658627</institution-id><institution>Institut für Hygiene und Infektionskrankheiten der Tiere,</institution><institution>Justus-Liebig Universität Giessen,</institution></institution-wrap>Giessen, Federal Republic of Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Giessen</wicri:regionArea></affiliation></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. 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Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.8664.c</institution-id><institution-id institution-id-type="ISNI">0000000121658627</institution-id><institution>Institut für Hygiene und Infektionskrankheiten der Tiere,</institution><institution>Justus-Liebig Universität Giessen,</institution></institution-wrap>Giessen, Federal Republic of Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Giessen</wicri:regionArea></affiliation></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. Kousoulas</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author><author><name sortKey="Storz, J" sort="Storz, J" uniqKey="Storz J" first="J." last="Storz">J. Storz</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author></analytic><series><title level="j">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><date when="1994">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author><author><name sortKey="Brian, Da" uniqKey="Brian D">DA Brian</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name></author><author><name sortKey="Lai, Mm" uniqKey="Lai M">MM Lai</name></author><author><name sortKey="Laude, H" uniqKey="Laude H">H Laude</name></author><author><name sortKey="Siddell, Sg" uniqKey="Siddell S">SG Siddell</name></author><author><name sortKey="Spaan, W" uniqKey="Spaan W">W Spaan</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F Taguchi</name></author><author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ 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Storz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Xm" uniqKey="Zhang X">XM Zhang</name></author><author><name sortKey="Kousoulas, Kg" uniqKey="Kousoulas K">KG Kousoulas</name></author><author><name sortKey="Storz, J" uniqKey="Storz J">J Storz</name></author></analytic></biblStruct></listBibl></div1></back></TEI></PMC></double></record>Pour manipuler ce document sous Unix (Dilib)
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