Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses
Identifieur interne : 000062 ( Pmc/Curation ); précédent : 000061; suivant : 000063Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses
Auteurs : X. Zhang ; W. Herbst [Allemagne] ; K. G. Kousoulas ; J. StorzSource :
- Archives of Virology [ 0304-8608 ] ; 1994.
Abstract
The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.
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DOI: 10.1007/BF01310579
PubMed: 8129626
PubMed Central: 7087011
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X. Zhang<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses</title><author><name sortKey="Zhang, X" sort="Zhang, X" uniqKey="Zhang X" first="X." last="Zhang">X. Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.8664.c</institution-id><institution-id institution-id-type="ISNI">0000000121658627</institution-id><institution>Institut für Hygiene und Infektionskrankheiten der Tiere,</institution><institution>Justus-Liebig Universität Giessen,</institution></institution-wrap>Giessen, Federal Republic of Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Giessen</wicri:regionArea></affiliation></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. 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Storz</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">8129626</idno><idno type="pmc">7087011</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7087011</idno><idno type="RBID">PMC:7087011</idno><idno type="doi">10.1007/BF01310579</idno><date when="1994">1994</date><idno type="wicri:Area/Pmc/Corpus">000062</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000062</idno><idno type="wicri:Area/Pmc/Curation">000062</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000062</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses</title><author><name sortKey="Zhang, X" sort="Zhang, X" uniqKey="Zhang X" first="X." last="Zhang">X. Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author><author><name sortKey="Herbst, W" sort="Herbst, W" uniqKey="Herbst W" first="W." last="Herbst">W. Herbst</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.8664.c</institution-id><institution-id institution-id-type="ISNI">0000000121658627</institution-id><institution>Institut für Hygiene und Infektionskrankheiten der Tiere,</institution><institution>Justus-Liebig Universität Giessen,</institution></institution-wrap>Giessen, Federal Republic of Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Giessen</wicri:regionArea></affiliation></author><author><name sortKey="Kousoulas, K G" sort="Kousoulas, K G" uniqKey="Kousoulas K" first="K. G." last="Kousoulas">K. G. Kousoulas</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author><author><name sortKey="Storz, J" sort="Storz, J" uniqKey="Storz J" first="J." last="Storz">J. Storz</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</nlm:aff><wicri:noCountry code="subfield">Louisiana USA</wicri:noCountry></affiliation></author></analytic><series><title level="j">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><date when="1994">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name></author><author><name sortKey="Brian, Da" uniqKey="Brian D">DA Brian</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name></author><author><name sortKey="Lai, Mm" uniqKey="Lai M">MM Lai</name></author><author><name sortKey="Laude, H" uniqKey="Laude H">H Laude</name></author><author><name sortKey="Siddell, Sg" uniqKey="Siddell S">SG Siddell</name></author><author><name sortKey="Spaan, W" uniqKey="Spaan W">W Spaan</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F Taguchi</name></author><author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Arch Virol</journal-id><journal-id journal-id-type="iso-abbrev">Arch. Virol</journal-id><journal-title-group><journal-title>Archives of Virology</journal-title></journal-title-group><issn pub-type="ppub">0304-8608</issn><issn pub-type="epub">1432-8798</issn><publisher><publisher-name>Springer-Verlag</publisher-name><publisher-loc>Vienna</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">8129626</article-id><article-id pub-id-type="pmc">7087011</article-id><article-id pub-id-type="publisher-id">BF01310579</article-id><article-id pub-id-type="doi">10.1007/BF01310579</article-id><article-categories><subj-group subj-group-type="heading"><subject>Brief Report</subject></subj-group></article-categories><title-group><article-title>Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>X.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Herbst</surname><given-names>W.</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Kousoulas</surname><given-names>K. G.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Storz</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.64337.35</institution-id><institution-id institution-id-type="ISNI">0000000106627451</institution-id><institution>Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine,</institution><institution>Louisiana State University,</institution></institution-wrap>Baton Rouge, Louisiana USA</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.8664.c</institution-id><institution-id institution-id-type="ISNI">0000000121658627</institution-id><institution>Institut für Hygiene und Infektionskrankheiten der Tiere,</institution><institution>Justus-Liebig Universität Giessen,</institution></institution-wrap>Giessen, Federal Republic of Germany</aff></contrib-group><pub-date pub-type="ppub"><year>1994</year></pub-date><volume>134</volume><issue>3</issue><fpage>421</fpage><lpage>426</lpage><history><date date-type="received"><day>23</day><month>6</month><year>1993</year></date><date date-type="accepted"><day>7</day><month>9</month><year>1993</year></date></history><permissions><copyright-statement>© Springer-Verlag 1994</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><title>Summary</title><p>The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Nucleotide</kwd><kwd>Western Blot</kwd><kwd>Monoclonal Antibody</kwd><kwd>Infectious Disease</kwd><kwd>Sequence Analysis</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer-Verlag 1994</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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