Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies
Identifieur interne : 001991 ( Main/Merge ); précédent : 001990; suivant : 001992Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies
Auteurs : H. Kasai [Japon] ; E. Morita [Japon] ; K. Hatakeyama [Japon] ; K. Sugiyama [Japon]Source :
- Archives of virology [ 0304-8608 ] ; 1998.
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Abstract
We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3a28. 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5a3, 6a6, 13a4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.
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Morita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author><author><name sortKey="Hatakeyama, K" sort="Hatakeyama, K" uniqKey="Hatakeyama K" first="K." last="Hatakeyama">K. Hatakeyama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author><author><name sortKey="Sugiyama, K" sort="Sugiyama, K" uniqKey="Sugiyama K" first="K." last="Sugiyama">K. Sugiyama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">99-0084586</idno><date when="1998">1998</date><idno type="stanalyst">PASCAL 99-0084586 INIST</idno><idno type="RBID">Pascal:99-0084586</idno><idno type="wicri:Area/PascalFrancis/Corpus">000060</idno><idno type="wicri:Area/PascalFrancis/Curation">000070</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000060</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000060</idno><idno type="wicri:doubleKey">0304-8608:1998:Kasai H:characterization:of:haemagglutinin</idno><idno type="wicri:Area/Main/Merge">001991</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies</title><author><name sortKey="Kasai, H" sort="Kasai, H" uniqKey="Kasai H" first="H." last="Kasai">H. Kasai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author><author><name sortKey="Morita, E" sort="Morita, E" uniqKey="Morita E" first="E." last="Morita">E. Morita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author><author><name sortKey="Hatakeyama, K" sort="Hatakeyama, K" uniqKey="Hatakeyama K" first="K." last="Hatakeyama">K. Hatakeyama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author><author><name sortKey="Sugiyama, K" sort="Sugiyama, K" uniqKey="Sugiyama K" first="K." last="Sugiyama">K. Sugiyama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biology, Faculty of Science, Hirosaki University</s1><s2>Hirosaki</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Hirosaki</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Archives of virology</title><title level="j" type="abbreviated">Arch. virol.</title><idno type="ISSN">0304-8608</idno><imprint><date when="1998">1998</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Archives of virology</title><title level="j" type="abbreviated">Arch. virol.</title><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigen</term><term>Antigenic determinant</term><term>Characterization</term><term>Esterases</term><term>Hemagglutinin</term><term>Monoclonal antibody</term><term>Murine hepatitis virus</term><term>Protein</term><term>Virus surface</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus hépatite murine</term><term>Protéine</term><term>Hémagglutinine</term><term>Esterases</term><term>Caractérisation</term><term>Anticorps monoclonal</term><term>Antigène</term><term>Déterminant antigénique</term><term>Surface virus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3a28. 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5a3, 6a6, 13a4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Kasai, H" sort="Kasai, H" uniqKey="Kasai H" first="H." last="Kasai">H. Kasai</name></noRegion><name sortKey="Hatakeyama, K" sort="Hatakeyama, K" uniqKey="Hatakeyama K" first="K." last="Hatakeyama">K. Hatakeyama</name><name sortKey="Morita, E" sort="Morita, E" uniqKey="Morita E" first="E." last="Morita">E. Morita</name><name sortKey="Sugiyama, K" sort="Sugiyama, K" uniqKey="Sugiyama K" first="K." last="Sugiyama">K. Sugiyama</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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