CovidV2, Main, Exploration, bibRecord, 001460

RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)

Identifieur interne : 001460 ( Main/Exploration ); précédent : 001459; suivant : 001461

RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)

Auteurs : Wan-Cheol Kim ; Dustin King ; Chow H. Lee

Source :

International Journal of Biochemistry and Molecular Biology [ 2152-4114 ] ; 2010.

RBID : PMC:3180037

Abstract

We have recently identified apurinic/apyrimidinic endonuclease 1 (APE1) as an endoribonuclease that cleaves c-myc mRNA in vitro and regulates c-myc mRNA levels and half-life in cells. This study was undertaken to further unravel the RNA-cleaving properties of APE1. Here, we show that APE1 cleaves RNA in the absence of divalent metal ions and, at 2 mM, Zn²⁺, Ni²⁺, Cu²⁺, or Co²⁺ inhibited the endoribonuclease activity of APE1. APE1 is able to cleave CD44 mRNA, microRNAs (miR-21, miR-10b), and three RNA components of SARS-corona virus (orf1b, orf3, spike) suggesting that, when challenged, it can cleave any RNAs in vitro. APE1 does not cleave strong doublestranded regions of RNA and it has a strong preference for 3’ of pyrimidine, especially towards UA, CA, and UG sites at single-stranded or weakly paired regions. It also cleaves RNA weakly at UC, CU, AC, and AU sites in single-stranded or weakly paired regions. Finally, we found that APE1 can reduce the ability of the Dicer enzyme to process premiRNAs in vitro. Overall, this study has revealed some previously unknown biochemical properties of APE1 which has implications for its role in vivo.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180037

PubMed: 21968700
PubMed Central: 3180037

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)</title>
<author><name sortKey="Kim, Wan Cheol" sort="Kim, Wan Cheol" uniqKey="Kim W" first="Wan-Cheol" last="Kim">Wan-Cheol Kim</name>
</author>
<author><name sortKey="King, Dustin" sort="King, Dustin" uniqKey="King D" first="Dustin" last="King">Dustin King</name>
</author>
<author><name sortKey="Lee, Chow H" sort="Lee, Chow H" uniqKey="Lee C" first="Chow H." last="Lee">Chow H. Lee</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21968700</idno>
<idno type="pmc">3180037</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180037</idno>
<idno type="RBID">PMC:3180037</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">000695</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000695</idno>
<idno type="wicri:Area/Pmc/Curation">000695</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000695</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000841</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000841</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:21968700</idno>
<idno type="wicri:Area/PubMed/Corpus">000D35</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D35</idno>
<idno type="wicri:Area/PubMed/Curation">000D35</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000D35</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000D15</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000D15</idno>
<idno type="wicri:Area/Ncbi/Merge">000286</idno>
<idno type="wicri:Area/Ncbi/Curation">000286</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000286</idno>
<idno type="wicri:Area/Main/Merge">001462</idno>
<idno type="wicri:Area/Main/Curation">001460</idno>
<idno type="wicri:Area/Main/Exploration">001460</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)</title>
<author><name sortKey="Kim, Wan Cheol" sort="Kim, Wan Cheol" uniqKey="Kim W" first="Wan-Cheol" last="Kim">Wan-Cheol Kim</name>
</author>
<author><name sortKey="King, Dustin" sort="King, Dustin" uniqKey="King D" first="Dustin" last="King">Dustin King</name>
</author>
<author><name sortKey="Lee, Chow H" sort="Lee, Chow H" uniqKey="Lee C" first="Chow H." last="Lee">Chow H. Lee</name>
</author>
</analytic>
<series><title level="j">International Journal of Biochemistry and Molecular Biology</title>
<idno type="eISSN">2152-4114</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>We have recently identified apurinic/apyrimidinic endonuclease 1 (APE1) as an endoribonuclease that cleaves c-<italic>myc</italic>
 mRNA <italic>in vitro</italic>
 and regulates c-<italic>myc</italic>
 mRNA levels and half-life in cells. This study was undertaken to further unravel the RNA-cleaving properties of APE1. Here, we show that APE1 cleaves RNA in the absence of divalent metal ions and, at 2 mM, Zn<sup>2+</sup>
, Ni<sup>2+</sup>
, Cu<sup>2+</sup>
, or Co<sup>2+</sup>
 inhibited the endoribonuclease activity of APE1. APE1 is able to cleave CD44 mRNA, microRNAs (miR-21, miR-10b), and three RNA components of SARS-corona virus (orf1b, orf3, spike) suggesting that, when challenged, it can cleave any RNAs <italic>in vitro</italic>
. APE1 does not cleave strong doublestranded regions of RNA and it has a strong preference for 3’ of pyrimidine, especially towards UA, CA, and UG sites at single-stranded or weakly paired regions. It also cleaves RNA weakly at UC, CU, AC, and AU sites in single-stranded or weakly paired regions. Finally, we found that APE1 can reduce the ability of the Dicer enzyme to process premiRNAs <italic>in vitro</italic>
. Overall, this study has revealed some previously unknown biochemical properties of APE1 which has implications for its role <italic>in vivo</italic>
.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Kim, Wan Cheol" sort="Kim, Wan Cheol" uniqKey="Kim W" first="Wan-Cheol" last="Kim">Wan-Cheol Kim</name>
<name sortKey="King, Dustin" sort="King, Dustin" uniqKey="King D" first="Dustin" last="King">Dustin King</name>
<name sortKey="Lee, Chow H" sort="Lee, Chow H" uniqKey="Lee C" first="Chow H." last="Lee">Chow H. Lee</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001460 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001460 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    CovidV2
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3180037
   |texte=   RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:21968700" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a CovidV2

This area was generated with Dilib version V0.6.33.
Data generation: Sat Mar 28 17:51:24 2020. Site generation: Sun Jan 31 15:35:48 2021

Serveur d'exploration Covid (26 mars)

RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)

RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration Covid (26 mars)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.