RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)
Identifieur interne : 000841 ( Pmc/Checkpoint ); précédent : 000840; suivant : 000842RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)
Auteurs : Wan-Cheol Kim ; Dustin King ; Chow H. LeeSource :
- International Journal of Biochemistry and Molecular Biology [ 2152-4114 ] ; 2010.
Abstract
We have recently identified apurinic/apyrimidinic endonuclease 1 (APE1) as an endoribonuclease that cleaves c-
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PubMed: 21968700
PubMed Central: 3180037
Affiliations:
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<author><name sortKey="Kim, Wan Cheol" sort="Kim, Wan Cheol" uniqKey="Kim W" first="Wan-Cheol" last="Kim">Wan-Cheol Kim</name>
</author>
<author><name sortKey="King, Dustin" sort="King, Dustin" uniqKey="King D" first="Dustin" last="King">Dustin King</name>
</author>
<author><name sortKey="Lee, Chow H" sort="Lee, Chow H" uniqKey="Lee C" first="Chow H." last="Lee">Chow H. Lee</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)</title>
<author><name sortKey="Kim, Wan Cheol" sort="Kim, Wan Cheol" uniqKey="Kim W" first="Wan-Cheol" last="Kim">Wan-Cheol Kim</name>
</author>
<author><name sortKey="King, Dustin" sort="King, Dustin" uniqKey="King D" first="Dustin" last="King">Dustin King</name>
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<author><name sortKey="Lee, Chow H" sort="Lee, Chow H" uniqKey="Lee C" first="Chow H." last="Lee">Chow H. Lee</name>
</author>
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<series><title level="j">International Journal of Biochemistry and Molecular Biology</title>
<idno type="eISSN">2152-4114</idno>
<imprint><date when="2010">2010</date>
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<front><div type="abstract" xml:lang="en"><p>We have recently identified apurinic/apyrimidinic endonuclease 1 (APE1) as an endoribonuclease that cleaves c-<italic>myc</italic>
mRNA <italic>in vitro</italic>
and regulates c-<italic>myc</italic>
mRNA levels and half-life in cells. This study was undertaken to further unravel the RNA-cleaving properties of APE1. Here, we show that APE1 cleaves RNA in the absence of divalent metal ions and, at 2 mM, Zn<sup>2+</sup>
, Ni<sup>2+</sup>
, Cu<sup>2+</sup>
, or Co<sup>2+</sup>
inhibited the endoribonuclease activity of APE1. APE1 is able to cleave CD44 mRNA, microRNAs (miR-21, miR-10b), and three RNA components of SARS-corona virus (orf1b, orf3, spike) suggesting that, when challenged, it can cleave any RNAs <italic>in vitro</italic>
. APE1 does not cleave strong doublestranded regions of RNA and it has a strong preference for 3’ of pyrimidine, especially towards UA, CA, and UG sites at single-stranded or weakly paired regions. It also cleaves RNA weakly at UC, CU, AC, and AU sites in single-stranded or weakly paired regions. Finally, we found that APE1 can reduce the ability of the Dicer enzyme to process premiRNAs <italic>in vitro</italic>
. Overall, this study has revealed some previously unknown biochemical properties of APE1 which has implications for its role <italic>in vivo</italic>
.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Biochem Mol Biol</journal-id>
<journal-id journal-id-type="publisher-id">ijbmb</journal-id>
<journal-title-group><journal-title>International Journal of Biochemistry and Molecular Biology</journal-title>
</journal-title-group>
<issn pub-type="epub">2152-4114</issn>
<publisher><publisher-name>e-Century Publishing Corporation</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21968700</article-id>
<article-id pub-id-type="pmc">3180037</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>RNA-cleaving properties of human apurinic/apyrimidinic endonuclease 1 (APE1)</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kim</surname>
<given-names>Wan-Cheol</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>King</surname>
<given-names>Dustin</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Lee</surname>
<given-names>Chow H.</given-names>
</name>
</contrib>
<aff><institution>Chemistry Program, University of Northern British Columbia, 3333 University Way</institution>
<addr-line>Prince George, BC V2N 4Z9</addr-line>
<country>Canada</country>
</aff>
</contrib-group>
<author-notes><corresp>Please address correspondence to: Chow H. Lee, PhD, <institution>Chemistry Program, University of Northern British Columbia</institution>
<addr-line>3333 University Way, Prince George, BC V2N 4Z9</addr-line>
<country>Canada</country>
. Phone: + 1 250 960 5413; Fax: + 1 250 960 5170; E-mail: <email>leec@unbc.ca</email>
.</corresp>
</author-notes>
<pub-date pub-type="collection"><year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>10</day>
<month>3</month>
<year>2010</year>
</pub-date>
<volume>1</volume>
<issue>1</issue>
<fpage>12</fpage>
<lpage>25</lpage>
<history><date date-type="received"><day>11</day>
<month>2</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>03</day>
<month>3</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>IJBMB Copyright © 2010</copyright-statement>
<copyright-year>2010</copyright-year>
</permissions>
<abstract><p>We have recently identified apurinic/apyrimidinic endonuclease 1 (APE1) as an endoribonuclease that cleaves c-<italic>myc</italic>
mRNA <italic>in vitro</italic>
and regulates c-<italic>myc</italic>
mRNA levels and half-life in cells. This study was undertaken to further unravel the RNA-cleaving properties of APE1. Here, we show that APE1 cleaves RNA in the absence of divalent metal ions and, at 2 mM, Zn<sup>2+</sup>
, Ni<sup>2+</sup>
, Cu<sup>2+</sup>
, or Co<sup>2+</sup>
inhibited the endoribonuclease activity of APE1. APE1 is able to cleave CD44 mRNA, microRNAs (miR-21, miR-10b), and three RNA components of SARS-corona virus (orf1b, orf3, spike) suggesting that, when challenged, it can cleave any RNAs <italic>in vitro</italic>
. APE1 does not cleave strong doublestranded regions of RNA and it has a strong preference for 3’ of pyrimidine, especially towards UA, CA, and UG sites at single-stranded or weakly paired regions. It also cleaves RNA weakly at UC, CU, AC, and AU sites in single-stranded or weakly paired regions. Finally, we found that APE1 can reduce the ability of the Dicer enzyme to process premiRNAs <italic>in vitro</italic>
. Overall, this study has revealed some previously unknown biochemical properties of APE1 which has implications for its role <italic>in vivo</italic>
.</p>
</abstract>
<kwd-group><kwd>Endoribonuclease</kwd>
<kwd>APE1</kwd>
<kwd>CD44</kwd>
<kwd>miRNA</kwd>
<kwd>RNA structure</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Kim, Wan Cheol" sort="Kim, Wan Cheol" uniqKey="Kim W" first="Wan-Cheol" last="Kim">Wan-Cheol Kim</name>
<name sortKey="King, Dustin" sort="King, Dustin" uniqKey="King D" first="Dustin" last="King">Dustin King</name>
<name sortKey="Lee, Chow H" sort="Lee, Chow H" uniqKey="Lee C" first="Chow H." last="Lee">Chow H. Lee</name>
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