Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures
Identifieur interne : 001751 ( Main/Merge ); précédent : 001750; suivant : 001752Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures
Auteurs : K. Andries [Belgique] ; M. Pensaert [Belgique]Source :
- Zentralblatt für Veterinärmedizin Reihe B [ 0931-2021 ] ; 1980-05.
English descriptors
- Teeft :
- Andries, Blind passage, Blood cells, Cell cultures, Cell line, Cell lines, Cell type, Cellules primaires, Clinical specimens, Cytopathic effect, Days post inoculation, Different cell cultures, Different criteria, Encephalomyelitis, Equal volume, Growth curve, Hads, Hemadsorption, Hemagglutinating, Hemagglutinating activity, Hemagglutinating encephalomyelitis virus, Hemagglutinating virus, Hemagglutination, Infectious virus, Infective virus, Infectivity, Infectivity titers, Infectivity titrations, Inoculated, Inoculation, Kidney cells, Lung homogenate stock, Maintenance medium, Microtiter plates, Monolayers, Optimal procedure, Optimal time, Pensaert, Porcine cell cultures, Present study, Same medium, Serial dilutions, Sheeted monolayers, Small amounts, Small foci, Spth, Spth cells, Supernatant, Supernatant fluid, Swine kidney, Swine kidney cell line, Swine testicle, Syncytium, Thyroid cells, Tissue culture stock, Titration, Transmissible gastroenteritis virus, Tubes inoculated, Veterinary medicine, Viral, Viral growth, Virus, Virus growth curves, Virus isolation, Virus isolation trials, Virus quantitation trials.
Abstract
This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.
Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien. Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.
Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles. Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.
Url:
DOI: 10.1111/j.1439-0450.1980.tb01693.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000509
- to stream Istex, to step Curation: 000489
- to stream Istex, to step Checkpoint: 000829
Links to Exploration step
ISTEX:70BEDF2F9B697BFF29044C6AA64F84782B378CECLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures</title><author><name sortKey="Andries, K" sort="Andries, K" uniqKey="Andries K" first="K." last="Andries">K. Andries</name></author><author><name sortKey="Pensaert, M" sort="Pensaert, M" uniqKey="Pensaert M" first="M." last="Pensaert">M. Pensaert</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:70BEDF2F9B697BFF29044C6AA64F84782B378CEC</idno><date when="1980" year="1980">1980</date><idno type="doi">10.1111/j.1439-0450.1980.tb01693.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-SFZBH9ZN-K/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000509</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000509</idno><idno type="wicri:Area/Istex/Curation">000489</idno><idno type="wicri:Area/Istex/Checkpoint">000829</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000829</idno><idno type="wicri:doubleKey">0931-2021:1980:Andries K:propagation:of:hemagglutinating</idno><idno type="wicri:Area/Main/Merge">001751</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures</title><author><name sortKey="Andries, K" sort="Andries, K" uniqKey="Andries K" first="K." last="Andries">K. Andries</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Correspondence address: Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent</wicri:regionArea><wicri:noRegion>B‐9000 Gent</wicri:noRegion></affiliation></author><author><name sortKey="Pensaert, M" sort="Pensaert, M" uniqKey="Pensaert M" first="M." last="Pensaert">M. Pensaert</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Correspondence address: Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent</wicri:regionArea><wicri:noRegion>B‐9000 Gent</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Zentralblatt für Veterinärmedizin Reihe B</title><title level="j" type="alt">ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B</title><idno type="ISSN">0931-2021</idno><idno type="eISSN">1439-0450</idno><imprint><biblScope unit="vol">27</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="280">280</biblScope><biblScope unit="page" to="290">290</biblScope><biblScope unit="page-count">11</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1980-05">1980-05</date></imprint><idno type="ISSN">0931-2021</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0931-2021</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Andries</term><term>Blind passage</term><term>Blood cells</term><term>Cell cultures</term><term>Cell line</term><term>Cell lines</term><term>Cell type</term><term>Cellules primaires</term><term>Clinical specimens</term><term>Cytopathic effect</term><term>Days post inoculation</term><term>Different cell cultures</term><term>Different criteria</term><term>Encephalomyelitis</term><term>Equal volume</term><term>Growth curve</term><term>Hads</term><term>Hemadsorption</term><term>Hemagglutinating</term><term>Hemagglutinating activity</term><term>Hemagglutinating encephalomyelitis virus</term><term>Hemagglutinating virus</term><term>Hemagglutination</term><term>Infectious virus</term><term>Infective virus</term><term>Infectivity</term><term>Infectivity titers</term><term>Infectivity titrations</term><term>Inoculated</term><term>Inoculation</term><term>Kidney cells</term><term>Lung homogenate stock</term><term>Maintenance medium</term><term>Microtiter plates</term><term>Monolayers</term><term>Optimal procedure</term><term>Optimal time</term><term>Pensaert</term><term>Porcine cell cultures</term><term>Present study</term><term>Same medium</term><term>Serial dilutions</term><term>Sheeted monolayers</term><term>Small amounts</term><term>Small foci</term><term>Spth</term><term>Spth cells</term><term>Supernatant</term><term>Supernatant fluid</term><term>Swine kidney</term><term>Swine kidney cell line</term><term>Swine testicle</term><term>Syncytium</term><term>Thyroid cells</term><term>Tissue culture stock</term><term>Titration</term><term>Transmissible gastroenteritis virus</term><term>Tubes inoculated</term><term>Veterinary medicine</term><term>Viral</term><term>Viral growth</term><term>Virus</term><term>Virus growth curves</term><term>Virus isolation</term><term>Virus isolation trials</term><term>Virus quantitation trials</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.</div><div type="abstract" xml:lang="de">Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien. Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.</div><div type="abstract" xml:lang="es">Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles. Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001751 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001751 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:70BEDF2F9B697BFF29044C6AA64F84782B378CEC
|texte= Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures
}}
| This area was generated with Dilib version V0.6.33. |  |