Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures
Identifieur interne : 000509 ( Istex/Corpus ); précédent : 000508; suivant : 000510Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures
Auteurs : K. Andries ; M. PensaertSource :
- Zentralblatt für Veterinärmedizin Reihe B [ 0931-2021 ] ; 1980-05.
English descriptors
- Teeft :
- Andries, Blind passage, Blood cells, Cell cultures, Cell line, Cell lines, Cell type, Cellules primaires, Clinical specimens, Cytopathic effect, Days post inoculation, Different cell cultures, Different criteria, Encephalomyelitis, Equal volume, Growth curve, Hads, Hemadsorption, Hemagglutinating, Hemagglutinating activity, Hemagglutinating encephalomyelitis virus, Hemagglutinating virus, Hemagglutination, Infectious virus, Infective virus, Infectivity, Infectivity titers, Infectivity titrations, Inoculated, Inoculation, Kidney cells, Lung homogenate stock, Maintenance medium, Microtiter plates, Monolayers, Optimal procedure, Optimal time, Pensaert, Porcine cell cultures, Present study, Same medium, Serial dilutions, Sheeted monolayers, Small amounts, Small foci, Spth, Spth cells, Supernatant, Supernatant fluid, Swine kidney, Swine kidney cell line, Swine testicle, Syncytium, Thyroid cells, Tissue culture stock, Titration, Transmissible gastroenteritis virus, Tubes inoculated, Veterinary medicine, Viral, Viral growth, Virus, Virus growth curves, Virus isolation, Virus isolation trials, Virus quantitation trials.
Abstract
This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.
Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien. Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.
Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles. Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.
Url:
DOI: 10.1111/j.1439-0450.1980.tb01693.x
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Andries</name><affiliation><mods:affiliation>Laboratory of Virology, Faculty of Veterinary Medicine, Gent</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent, Belgium.</mods:affiliation></affiliation></author><author><name sortKey="Pensaert, M" sort="Pensaert, M" uniqKey="Pensaert M" first="M." last="Pensaert">M. Pensaert</name><affiliation><mods:affiliation>Laboratory of Virology, Faculty of Veterinary Medicine, Gent</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent, Belgium.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Zentralblatt für Veterinärmedizin Reihe B</title><title level="j" type="alt">ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B</title><idno type="ISSN">0931-2021</idno><idno type="eISSN">1439-0450</idno><imprint><biblScope unit="vol">27</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="280">280</biblScope><biblScope unit="page" to="290">290</biblScope><biblScope unit="page-count">11</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1980-05">1980-05</date></imprint><idno type="ISSN">0931-2021</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0931-2021</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Andries</term><term>Blind passage</term><term>Blood cells</term><term>Cell cultures</term><term>Cell line</term><term>Cell lines</term><term>Cell type</term><term>Cellules primaires</term><term>Clinical specimens</term><term>Cytopathic effect</term><term>Days post inoculation</term><term>Different cell cultures</term><term>Different criteria</term><term>Encephalomyelitis</term><term>Equal volume</term><term>Growth curve</term><term>Hads</term><term>Hemadsorption</term><term>Hemagglutinating</term><term>Hemagglutinating activity</term><term>Hemagglutinating encephalomyelitis virus</term><term>Hemagglutinating virus</term><term>Hemagglutination</term><term>Infectious virus</term><term>Infective virus</term><term>Infectivity</term><term>Infectivity titers</term><term>Infectivity titrations</term><term>Inoculated</term><term>Inoculation</term><term>Kidney cells</term><term>Lung homogenate stock</term><term>Maintenance medium</term><term>Microtiter plates</term><term>Monolayers</term><term>Optimal procedure</term><term>Optimal time</term><term>Pensaert</term><term>Porcine cell cultures</term><term>Present study</term><term>Same medium</term><term>Serial dilutions</term><term>Sheeted monolayers</term><term>Small amounts</term><term>Small foci</term><term>Spth</term><term>Spth cells</term><term>Supernatant</term><term>Supernatant fluid</term><term>Swine kidney</term><term>Swine kidney cell line</term><term>Swine testicle</term><term>Syncytium</term><term>Thyroid cells</term><term>Tissue culture stock</term><term>Titration</term><term>Transmissible gastroenteritis virus</term><term>Tubes inoculated</term><term>Veterinary medicine</term><term>Viral</term><term>Viral growth</term><term>Virus</term><term>Virus growth curves</term><term>Virus isolation</term><term>Virus isolation trials</term><term>Virus quantitation trials</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.</div><div type="abstract" xml:lang="de">Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien. Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.</div><div type="abstract" xml:lang="es">Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles. Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>spth</json:string><json:string>hads</json:string><json:string>inoculated</json:string><json:string>encephalomyelitis</json:string><json:string>hemagglutinating</json:string><json:string>infectivity</json:string><json:string>viral</json:string><json:string>pensaert</json:string><json:string>monolayers</json:string><json:string>hemagglutination</json:string><json:string>hemagglutinating encephalomyelitis virus</json:string><json:string>andries</json:string><json:string>blind passage</json:string><json:string>syncytium</json:string><json:string>hemadsorption</json:string><json:string>titration</json:string><json:string>spth cells</json:string><json:string>tissue culture stock</json:string><json:string>cytopathic effect</json:string><json:string>supernatant fluid</json:string><json:string>different criteria</json:string><json:string>thyroid cells</json:string><json:string>cell cultures</json:string><json:string>growth curve</json:string><json:string>optimal time</json:string><json:string>cell line</json:string><json:string>viral growth</json:string><json:string>blood cells</json:string><json:string>infective virus</json:string><json:string>inoculation</json:string><json:string>supernatant</json:string><json:string>virus</json:string><json:string>maintenance medium</json:string><json:string>hemagglutinating virus</json:string><json:string>porcine cell cultures</json:string><json:string>cell lines</json:string><json:string>cell type</json:string><json:string>kidney cells</json:string><json:string>infectious virus</json:string><json:string>infectivity titers</json:string><json:string>virus isolation</json:string><json:string>virus quantitation trials</json:string><json:string>swine testicle</json:string><json:string>different cell cultures</json:string><json:string>microtiter plates</json:string><json:string>serial dilutions</json:string><json:string>equal volume</json:string><json:string>infectivity titrations</json:string><json:string>hemagglutinating activity</json:string><json:string>virus growth curves</json:string><json:string>veterinary medicine</json:string><json:string>small foci</json:string><json:string>lung homogenate stock</json:string><json:string>present study</json:string><json:string>swine kidney</json:string><json:string>swine kidney cell line</json:string><json:string>days post inoculation</json:string><json:string>tubes inoculated</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>virus isolation trials</json:string><json:string>same medium</json:string><json:string>small amounts</json:string><json:string>optimal procedure</json:string><json:string>clinical specimens</json:string><json:string>cellules primaires</json:string><json:string>sheeted monolayers</json:string></teeft></keywords><author><json:item><name>K. 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A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. 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<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><head>Summary</head><p>This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.</p><head>Zusammenfassung</head><p>Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen</p><p>Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien.</p><p>Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.</p><head>Résumé</head><p>Culture du virus hémagglutinant de l'encéphalomyélite (HEV) dans des cultures de cellules de porc</p><p>On décrit les propriétés de culture de la souche HEV VW 572 dans des cellules primaires de reins de pores (PPK), dans des cellules primaires de testicules de porcs (PPT), dans des cellules secondaires de glandes tyroïdes de porcs (SPTh) et dans les lignées cellulaires PK‐15 (reins de porcs), SK−6 (reins de porcs) et ST (testicules de porcs). Les courbes de multiplication établies avec l'effet cytopathique (CPE), l'immunofluorescence (IF), l'hémadsorption (Hads) et l'hémagglutination (HA) comme critères pour la réplication du virus ont montré que les cellules SPTh et PPK étaient les plus sensibles pour la culture et la titration du virus. CPE, Hads et HA furent les critères sensibles et utilisables pour la mise en évidence de la multiplication virale en tubes inoculés avec de petites quantités de virus.</p><p>Des titrations du virus répétées ont montré une forte variation du titre final également dans les cultures cellulaires hautement réceptrices. La méthode optimale pour l'isolement de HEV à partir d'un matériel clinique est l'inoculation sur SPTh ou PPK avec passages à l'aveugle si le test HA est négatif 7 jours après l'infection.</p><head>Resumen</head><p>Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos</p><p>Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles.</p><p>Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.</p></abstract><textClass><keywords rend="tocHeading1"><term>Original Article</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-SFZBH9ZN-K/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0450a</doi><issn type="print">0931-2021</issn><issn type="electronic">1439-0450</issn><idGroup><id type="product" value="ZPH"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B">Zentralblatt für Veterinärmedizin Reihe B</title></titleGroup></publicationMeta><publicationMeta level="part" position="05004"><doi origin="wiley">10.1111/zph.1980.27.issue-4</doi><numberingGroup><numbering type="journalVolume" number="27">27</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="1980-05">May 1980</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="3" status="forIssue"><doi origin="wiley">10.1111/j.1439-0450.1980.tb01693.x</doi><idGroup><id type="unit" value="ZPH1693"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Original Article</title></titleGroup><copyright>© 1980 Blackwell Verlag GmbH</copyright><eventGroup><event type="firstOnline" date="2010-05-13"></event><event type="publishedOnlineFinalForm" date="2010-05-13"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:HeaderRef result:HeaderRef" date="2010-05-17"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-11"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-11-04"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="280">280</numbering><numbering type="pageLast" number="290">290</numbering></numberingGroup><correspondenceTo>Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent, Belgium.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ZPH.ZPH1693.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received for publication November 13,1979</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="17"></count><count type="linksCrossRef" number="4"></count></countGroup><titleGroup><title type="main">Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>K.</givenNames><familyName>Andries</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" corresponding="yes"><personName><givenNames>M.</givenNames><familyName>Pensaert</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Laboratory of Virology, Faculty of Veterinary Medicine, Gent</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><section xml:id="abs1-1"><title type="main">Summary</title><p>This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.</p></section><section xml:id="abs1-2"><title type="main">Zusammenfassung</title><p>Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen</p><p>Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien.</p><p>Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.</p></section><section xml:id="abs1-3"><title type="main">Résumé</title><p>Culture du virus hémagglutinant de l'encéphalomyélite (HEV) dans des cultures de cellules de porc</p><p>On décrit les propriétés de culture de la souche HEV VW 572 dans des cellules primaires de reins de pores (PPK), dans des cellules primaires de testicules de porcs (PPT), dans des cellules secondaires de glandes tyroïdes de porcs (SPTh) et dans les lignées cellulaires PK‐15 (reins de porcs), SK−6 (reins de porcs) et ST (testicules de porcs). Les courbes de multiplication établies avec l'effet cytopathique (CPE), l'immunofluorescence (IF), l'hémadsorption (Hads) et l'hémagglutination (HA) comme critères pour la réplication du virus ont montré que les cellules SPTh et PPK étaient les plus sensibles pour la culture et la titration du virus. CPE, Hads et HA furent les critères sensibles et utilisables pour la mise en évidence de la multiplication virale en tubes inoculés avec de petites quantités de virus.</p><p>Des titrations du virus répétées ont montré une forte variation du titre final également dans les cultures cellulaires hautement réceptrices. La méthode optimale pour l'isolement de HEV à partir d'un matériel clinique est l'inoculation sur SPTh ou PPK avec passages à l'aveugle si le test HA est négatif 7 jours après l'infection.</p></section><section xml:id="abs1-4"><title type="main">Resumen</title><p>Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos</p><p>Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles.</p><p>Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.</p></section></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Propagation of Hemagglutinating Encephalomyelitis Virus in Porcine Cell Cultures</title></titleInfo><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Andries</namePart><affiliation>Laboratory of Virology, Faculty of Veterinary Medicine, Gent</affiliation><affiliation>Correspondence address: Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent, Belgium.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Pensaert</namePart><affiliation>Laboratory of Virology, Faculty of Veterinary Medicine, Gent</affiliation><affiliation>Correspondence address: Laboratory of Virology, Faculty of Veterinary Medicine, Casinoplein 24, B‐9000 Gent, Belgium.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1980-05</dateIssued><edition>Received for publication November 13,1979</edition><copyrightDate encoding="w3cdtf">1980</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">17</extent><extent unit="linksCrossRef">4</extent></physicalDescription><abstract lang="en">This study reports some cultural characteristics of the VW 572 strain of hemagglutinating encephalomyelitis virus (HEV) in primary pig kidney (PPK) cells, primary pig testicle (PPT) cells, secondary pig thyroid (SPTh) cells and the cell lines PK‐15 (pig kidney), SK−6 (swine kidney) and ST (swine testicle). A growth curve, based on cytopathic effect (CPE), immunofluorescence (IF), hemadsorption (Hads) and hemagglutination (HA), showed that SPTh and PPK cells were most susceptible for cultivation and quantitation of the virus. For the detection of replication in tubes inoculated with small amounts of virus, CPE, Hads and HA appeared to be useful and sensitive criteria. Repeated virus quantitation trials revealed a high variation in titration end‐points, even in the most susceptible cell types. The optimal procedure for the isolation of HEV from clinical specimens is preferably to inoculate the material on SPTh or PPK cells and to make a blind passage if the HA test at 7 days post inoculation is negative.</abstract><abstract lang="de">Züchtung von Hemagglutinating Encephalomyelitis Virus (HEV) in porcinen Zellkulturen Kulturelle Eigenschaften des HEV‐Stammes VW 572 in primären Schweinenierenzellen (PPK), primären Schweinehodenzellen (PPT), sekundären Schweineschilddrüsenzellen (SPTh) und in den Zellinien PK‐15 (Schweinenieren), SK−6 (Schweinenieren) und ST (Schweinehoden) werden beschrieben. Vermehrungskurven, die mit Hilfe des cytopatischen Effektes (CPE), der Immunofluoreszenz (IF), der Hämadsorption (Hads) und der Hämagglutination als Kriterien für die Virusreplikation erstellt wurden, zeigten, daß SPTh und PPK‐Zellen am empfindlichsten für die Züchtung und Titration des Virus sind. Zum Nachweis der Virusvermehrung in Röhrchen, die mit kleinen Virusmengen beimpft wurden, waren CPE, Hads und HA empfindliche und brauchbare Kriterien. Wiederholte Virustitrationen zeigten eine hohe Variation der Titerendpunkte auch in hochempfänglichen Zellkulturen. Die optimale Methode für die Isolierung von HEV von klinischem Material ist die Verimpfung auf SPTh oder PPK mit anschließender Blindpassage, wenn der HA‐Test sieben Tage p. inf. negativ ist.</abstract>Culture du virus hémagglutinant de l'encéphalomyélite (HEV) dans des cultures de cellules de porc On décrit les propriétés de culture de la souche HEV VW 572 dans des cellules primaires de reins de pores (PPK), dans des cellules primaires de testicules de porcs (PPT), dans des cellules secondaires de glandes tyroïdes de porcs (SPTh) et dans les lignées cellulaires PK‐15 (reins de porcs), SK−6 (reins de porcs) et ST (testicules de porcs). Les courbes de multiplication établies avec l'effet cytopathique (CPE), l'immunofluorescence (IF), l'hémadsorption (Hads) et l'hémagglutination (HA) comme critères pour la réplication du virus ont montré que les cellules SPTh et PPK étaient les plus sensibles pour la culture et la titration du virus. CPE, Hads et HA furent les critères sensibles et utilisables pour la mise en évidence de la multiplication virale en tubes inoculés avec de petites quantités de virus. Des titrations du virus répétées ont montré une forte variation du titre final également dans les cultures cellulaires hautement réceptrices. La méthode optimale pour l'isolement de HEV à partir d'un matériel clinique est l'inoculation sur SPTh ou PPK avec passages à l'aveugle si le test HA est négatif 7 jours après l'infection.<abstract lang="es">Propagación del virus hemoaglutinante de la encefalomielitis (HEV) en los cultivos de células de cerdos Se describen las propiedades culturales de la estirpe HEV VW 572 en células renales primarias de cerdo (PPK), células testiculares primarias de cerdo, células tiroideas secundarias de cerdo (SPTh) y en las líneas celulares PK‐15 (riñones de cerdo), SK−6 (riñones porcinos) y ST (testículos de cerdo). Las curvas de multiplicación, las cuales se establecieron con ayuda del efecto citopático (CPE), la inmunofluorescencia (IF), la hemoadsorción (Hads) y la hemoaglutinación como criterios para la replicación virósica, mostraban que las células SPTh y PPK son las más sensibles para el cultivo y titulación del virus. Para la puesta en evidencia de la multiplicación virósica en tubitos, los cuales se inocularon con cantidades pequeñas de virus, eran CPE, Hads y HA criterios sensibles y útiles. Titulaciones repetidas de virus mostraban una variación elevada de los puntos finales de títulos incluso en cultivos celulares harto receptibles. El método óptimo para el aislamiento de HEV a partir de material clínico consiste en la inoculación a SPTH o PPK con pase ciego inmediato si la prueba HA es negativa 7 días después de la infección.</abstract><relatedItem type="host"><titleInfo><title>Zentralblatt für Veterinärmedizin Reihe B</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0931-2021</identifier><identifier type="eISSN">1439-0450</identifier><identifier type="DOI">10.1111/(ISSN)1439-0450a</identifier><identifier type="PublisherID">ZPH</identifier><part><date>1980</date><detail type="volume"><caption>vol.</caption><number>27</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>280</start><end>290</end><total>11</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Viral encephalomyelitis of swine in Ontario — Experimental and natural transmission</title></titleInfo><name type="personal"><namePart type="given">T. J. L.</namePart><namePart type="family">Alexander</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Alexander, T. J. L., 1962: Viral encephalomyelitis of swine in Ontario — Experimental and natural transmission. Am. J. vet. Res. 23, 756–762.</note><part><date>1962</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>756</start><end>762</end></extent></part><relatedItem type="host"><titleInfo><title>Am. J. vet. Res.</title></titleInfo><part><date>1962</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><extent unit="pages"><start>756</start><end>762</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit2"><titleInfo><title>Pathogenicity of hemagglutinating encephalomyelitis (vomiting and wasting disease) virus of pigs, using different routes of inoculation</title></titleInfo><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Andries</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Pensaert</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Callebaut</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Andries, K., M. Pensaert, and P. Callebaut, 1978: Pathogenicity of hemagglutinating encephalomyelitis (vomiting and wasting disease) virus of pigs, using different routes of inoculation. Zbl. Vet. Med. B 25, 461–468.</note><part><date>1978</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><extent unit="pages"><start>461</start><end>468</end></extent></part><relatedItem type="host"><titleInfo><title>Zbl. Vet. Med. B</title></titleInfo><part><date>1978</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><extent unit="pages"><start>461</start><end>468</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit3"><titleInfo><title>Virus isolation and immunofluorescence in different organs of pigs infected with hemagglutinating encephalomyelitis virus</title></titleInfo><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Andries</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. B.</namePart><namePart type="family">Pensaert</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Andries, K., and M. B. Pensaert: Virus isolation and immunofluorescence in different organs of pigs infected with hemagglutinating encephalomyelitis virus. Am. J. vet. Res., in press.</note><relatedItem type="host"><titleInfo><title>Am. J. vet. Res.</title></titleInfo></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit4"><titleInfo><title>Vomiting and wasting disease of piglets</title></titleInfo><name type="personal"><namePart type="given">Sheila F.</namePart><namePart type="family">Cartwright</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Lucas</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. P.</namePart><namePart type="family">Cavill</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. F.</namePart><namePart type="family">Gush</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Blandford</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Cartwright, Sheila F., M. Lucas, J. P. Cavill, A. F. Gush, and T. Blandford, 1969: Vomiting and wasting disease of piglets. Vet. Rec. 84, 175–176.</note><part><date>1969</date><detail type="volume"><caption>vol.</caption><number>84</number></detail><extent unit="pages"><start>175</start><end>176</end></extent></part><relatedItem type="host"><titleInfo><title>Vet. Rec.</title></titleInfo><part><date>1969</date><detail type="volume"><caption>vol.</caption><number>84</number></detail><extent unit="pages"><start>175</start><end>176</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit5"><titleInfo><title>Isolement en France et identification de la maladie du vomissement et du dépérissement des porcelets (corona‐like virus)</title></titleInfo><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Chappuis</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Tektoff</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Turdu</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Chappuis, G., J. Tektoff, et Y. Turdu, 1975: Isolement en France et identification de la maladie du vomissement et du dépérissement des porcelets (corona‐like virus). Rec. Méd. Vét. 151, 557–566.</note><part><date>1975</date><detail type="volume"><caption>vol.</caption><number>151</number></detail><extent unit="pages"><start>557</start><end>566</end></extent></part><relatedItem type="host"><titleInfo><title>Rec. Méd. Vét.</title></titleInfo><part><date>1975</date><detail type="volume"><caption>vol.</caption><number>151</number></detail><extent unit="pages"><start>557</start><end>566</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit6"><titleInfo><title>An electron microscopic study of haemagglutinating encephalomyelitis virus of pigs</title></titleInfo><name type="personal"><namePart type="given">J. K.</namePart><namePart type="family">Clarke</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. B.</namePart><namePart type="family">McFerran</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Clarke, J. K., and J. B. McFerran, 1971: An electron microscopic study of haemagglutinating encephalomyelitis virus of pigs. J. Gen. Virol. 13, 339–344.</note><part><date>1971</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>339</start><end>344</end></extent></part><relatedItem type="host"><titleInfo><title>J. Gen. Virol.</title></titleInfo><part><date>1971</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>339</start><end>344</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit7"><titleInfo><title>pH stability studies with avian infectious bronchitis virus (corona‐virus) strains</title></titleInfo><name type="personal"><namePart type="given">B. S.</namePart><namePart type="family">Cowen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. B.</namePart><namePart type="family">Hitchner</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Cowen, B. S., and S. B. Hitchner, 1975: pH stability studies with avian infectious bronchitis virus (corona‐virus) strains. J. Virol. 15, 430–432.</note><part><date>1975</date><detail type="volume"><caption>vol.</caption><number>15</number></detail><extent unit="pages"><start>430</start><end>432</end></extent></part><relatedItem type="host"><titleInfo><title>J. Virol.</title></titleInfo><part><date>1975</date><detail type="volume"><caption>vol.</caption><number>15</number></detail><extent unit="pages"><start>430</start><end>432</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit8"><titleInfo><title>Encephalomyelitis of swine caused by a hemagglutinating virus. III. Serological studies</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Girard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. S.</namePart><namePart type="family">Greig</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Mitchell</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Girard, A., A. S. Greig, and D. Mitchell, 1964: Encephalomyelitis of swine caused by a hemagglutinating virus. III. Serological studies. Res. Vet. Sci. 5, 294–302.</note><part><date>1964</date><detail type="volume"><caption>vol.</caption><number>5</number></detail><extent unit="pages"><start>294</start><end>302</end></extent></part><relatedItem type="host"><titleInfo><title>Res. Vet. Sci.</title></titleInfo><part><date>1964</date><detail type="volume"><caption>vol.</caption><number>5</number></detail><extent unit="pages"><start>294</start><end>302</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit9"><titleInfo><title>A hemagglutinating virus producing encephalomyelitis in baby pigs</title></titleInfo><name type="personal"><namePart type="given">A. S.</namePart><namePart type="family">Greig</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Mitchell</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. H.</namePart><namePart type="family">Corner</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. L.</namePart><namePart type="family">Bannister</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E. B.</namePart><namePart type="family">Meads</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. J.</namePart><namePart type="family">Julian</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Greig, A. S., D. Mitchell, A. H. Corner, G. L. Bannister, E. B. Meads, and R. J. Julian, 1962: A hemagglutinating virus producing encephalomyelitis in baby pigs. Can. J. Comp. Med. 26, 49–56.</note><part><date>1962</date><detail type="volume"><caption>vol.</caption><number>26</number></detail><extent unit="pages"><start>49</start><end>56</end></extent></part><relatedItem type="host"><titleInfo><title>Can. J. Comp. Med.</title></titleInfo><part><date>1962</date><detail type="volume"><caption>vol.</caption><number>26</number></detail><extent unit="pages"><start>49</start><end>56</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit10"><titleInfo><title>Encephalomyelitis of swine caused by a hemagglutinating virus. VI. Morphology of the virus</title></titleInfo><name type="personal"><namePart type="given">A. S.</namePart><namePart type="family">Greig</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. M.</namePart><namePart type="family">Johnson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. M. P.</namePart><namePart type="family">Bouillant</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Greig, A. S., C. M. Johnson, and A. M. P. Bouillant, 1971: Encephalomyelitis of swine caused by a hemagglutinating virus. VI. Morphology of the virus. Res. Vet. Sci. 12, 305–307.</note><part><date>1971</date><detail type="volume"><caption>vol.</caption><number>12</number></detail><extent unit="pages"><start>305</start><end>307</end></extent></part><relatedItem type="host"><titleInfo><title>Res. Vet. Sci.</title></titleInfo><part><date>1971</date><detail type="volume"><caption>vol.</caption><number>12</number></detail><extent unit="pages"><start>305</start><end>307</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit11"><titleInfo><title>In vitro differentiation and pH sensitivity of field and cell culture attenuated strains of transmissible gastroenteritis virus</title></titleInfo><name type="personal"><namePart type="given">R. G.</namePart><namePart type="family">Hess</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. A.</namePart><namePart type="family">Bachmann</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Hess, R. G., and P. A. Bachmann, 1976: In vitro differentiation and pH sensitivity of field and cell culture attenuated strains of transmissible gastroenteritis virus. Inf. & Imm. 13, 1642–1646.</note><part><date>1976</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>1642</start><end>1646</end></extent></part><relatedItem type="host"><titleInfo><title>Inf. & Imm.</title></titleInfo><part><date>1976</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>1642</start><end>1646</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit12"><titleInfo><title>Establishment, viral susceptibility and biological characteristics of a swine kidney cell line SK−6</title></titleInfo><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Kasza</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. A.</namePart><namePart type="family">Shadduck</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. J.</namePart><namePart type="family">Cristofinis</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Kasza, L., J. A. Shadduck, and G. J. Cristofinis, 1972: Establishment, viral susceptibility and biological characteristics of a swine kidney cell line SK−6. Res. Vet. Sci. 13, 46–51.</note><part><date>1972</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>46</start><end>51</end></extent></part><relatedItem type="host"><titleInfo><title>Res. Vet. Sci.</title></titleInfo><part><date>1972</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><extent unit="pages"><start>46</start><end>51</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit13"><titleInfo><title>Lenette, E. H., and N. J. Schmidt, 1969: Diagnostic procedures for viral and rickettsial diseases, 4th Ed., p. 90. Amer. publ. Hlth. Ass.</title></titleInfo><note type="citation/reference">Lenette, E. H., and N. J. Schmidt, 1969: Diagnostic procedures for viral and rickettsial diseases, 4th Ed., p. 90. Amer. publ. Hlth. Ass.</note><name type="personal"><namePart type="given">E. H.</namePart><namePart type="family">Lenette</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N. J.</namePart><namePart type="family">Schmidt</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>book</genre><originInfo><publisher>Amer. publ. Hlth. Ass</publisher></originInfo><part><date>1969</date><extent unit="pages"><start>90</start></extent></part></relatedItem><relatedItem type="references" displayLabel="cit14"><titleInfo><title>Fluorescent antibody technique in the study of three porcine viruses transmissible gastroenteritis virus, vomiting and wasting virus and the parvovirus 59 E/63</title></titleInfo><name type="personal"><namePart type="given">Margaret H.</namePart><namePart type="family">Lucas</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Napthine</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Lucas, Margaret H., and P. Napthine, 1971: Fluorescent antibody technique in the study of three porcine viruses transmissible gastroenteritis virus, vomiting and wasting virus and the parvovirus 59 E/63. J. Comp. Path. 81, 111–117.</note><part><date>1971</date><detail type="volume"><caption>vol.</caption><number>81</number></detail><extent unit="pages"><start>111</start><end>117</end></extent></part><relatedItem type="host"><titleInfo><title>J. Comp. Path.</title></titleInfo><part><date>1971</date><detail type="volume"><caption>vol.</caption><number>81</number></detail><extent unit="pages"><start>111</start><end>117</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit15"><titleInfo><title>Characteristics of a coronavirus causing vomition and wasting in pigs</title></titleInfo><name type="personal"><namePart type="given">M. B.</namePart><namePart type="family">Pensaert</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. E.</namePart><namePart type="family">Callebaut</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Pensaert, M. B., and P. E. Callebaut, 1974: Characteristics of a coronavirus causing vomition and wasting in pigs. Arch. ges. Virusforsch. 44, 35–50.</note><part><date>1974</date><detail type="volume"><caption>vol.</caption><number>44</number></detail><extent unit="pages"><start>35</start><end>50</end></extent></part><relatedItem type="host"><titleInfo><title>Arch. ges. Virusforsch.</title></titleInfo><part><date>1974</date><detail type="volume"><caption>vol.</caption><number>44</number></detail><extent unit="pages"><start>35</start><end>50</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit16"><titleInfo><title>Titration of two porcine respiratory viruses in mammalian cell cultures by direct fluorescent antibody staining</title></titleInfo><name type="personal"><namePart type="given">E. C.</namePart><namePart type="family">Pirtle</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Pirtle, E. C., 1974: Titration of two porcine respiratory viruses in mammalian cell cultures by direct fluorescent antibody staining. Am. J. Vet. Res. 35, 249–250.</note><part><date>1974</date><detail type="volume"><caption>vol.</caption><number>35</number></detail><extent unit="pages"><start>249</start><end>250</end></extent></part><relatedItem type="host"><titleInfo><title>Am. J. Vet. Res.</title></titleInfo><part><date>1974</date><detail type="volume"><caption>vol.</caption><number>35</number></detail><extent unit="pages"><start>249</start><end>250</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit17"><titleInfo><title>A simple method of estimating fifty per cent endpoints</title></titleInfo><name type="personal"><namePart type="given">L. J.</namePart><namePart type="family">Reed</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Muench</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Reed, L. J., and H. Muench, 1938: A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27, 493–497.</note><part><date>1938</date><detail type="volume"><caption>vol.</caption><number>27</number></detail><extent unit="pages"><start>493</start><end>497</end></extent></part><relatedItem type="host"><titleInfo><title>Am. J. Hyg.</title></titleInfo><part><date>1938</date><detail type="volume"><caption>vol.</caption><number>27</number></detail><extent unit="pages"><start>493</start><end>497</end></extent></part></relatedItem></relatedItem><identifier type="istex">70BEDF2F9B697BFF29044C6AA64F84782B378CEC</identifier><identifier type="ark">ark:/67375/WNG-SFZBH9ZN-K</identifier><identifier type="DOI">10.1111/j.1439-0450.1980.tb01693.x</identifier><identifier type="ArticleID">ZPH1693</identifier><accessCondition type="use and reproduction" contentType="copyright">© 1980 Blackwell Verlag GmbH</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-17</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-SFZBH9ZN-K/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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