Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells
Identifieur interne : 001662 ( Main/Merge ); précédent : 001661; suivant : 001663Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells
Auteurs : Georg Pauli [Allemagne] ; Jens-Peter Gregersen [Allemagne] ; Hanns Ludwig [Allemagne]Source :
- Journal of Immunological Methods [ 0022-1759 ] ; 1984.
English descriptors
- Teeft :
- Antibody, Comparable results, Different fixatives, Fixation, Fixative, Fixative solution, Focus immunoassay, Glutaraldehyde fixation, Growth medium, Horseradish peroxidase, Immune reaction step, Immunoassay, Infectious units, Large scale screening, Microtitre, Microtitre plate, Microtitre plates, Monoclonal antibodies, Plaque, Plaque immunoassay, Secondary antibodies, Substrate solution, Test serum dilutions, Uninfected cells, Viral antigens.
Abstract
Abstract: A new, simple enzyme-linked immunosorbent assay (ELISA) is described which is performed directly on infected and fixed cell cultures in microtitre plates. It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. This immunoassay requires no special equipment and results may be evaluated either with the naked eye or using a light microscope.
Url:
DOI: 10.1016/0022-1759(84)90301-6
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000437
- to stream Istex, to step Curation: 000417
- to stream Istex, to step Checkpoint: 000762
Links to Exploration step
ISTEX:FF9244C8CA94116E1DE47356BA3DFA146211FA5ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells</title>
<author><name sortKey="Pauli, Georg" sort="Pauli, Georg" uniqKey="Pauli G" first="Georg" last="Pauli">Georg Pauli</name>
</author>
<author><name sortKey="Gregersen, Jens Peter" sort="Gregersen, Jens Peter" uniqKey="Gregersen J" first="Jens-Peter" last="Gregersen">Jens-Peter Gregersen</name>
</author>
<author><name sortKey="Ludwig, Hanns" sort="Ludwig, Hanns" uniqKey="Ludwig H" first="Hanns" last="Ludwig">Hanns Ludwig</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:FF9244C8CA94116E1DE47356BA3DFA146211FA5A</idno>
<date when="1984" year="1984">1984</date>
<idno type="doi">10.1016/0022-1759(84)90301-6</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-XJ6HGQ7V-N/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000437</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000437</idno>
<idno type="wicri:Area/Istex/Curation">000417</idno>
<idno type="wicri:Area/Istex/Checkpoint">000762</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000762</idno>
<idno type="wicri:doubleKey">0022-1759:1984:Pauli G:plaque:focus:immunoassay</idno>
<idno type="wicri:Area/Main/Merge">001662</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells</title>
<author><name sortKey="Pauli, Georg" sort="Pauli, Georg" uniqKey="Pauli G" first="Georg" last="Pauli">Georg Pauli</name>
<affiliation wicri:level="3"><country xml:lang="fr" wicri:curation="lc">Allemagne</country>
<wicri:regionArea>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gregersen, Jens Peter" sort="Gregersen, Jens Peter" uniqKey="Gregersen J" first="Jens-Peter" last="Gregersen">Jens-Peter Gregersen</name>
<affiliation wicri:level="3"><country xml:lang="fr" wicri:curation="lc">Allemagne</country>
<wicri:regionArea>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ludwig, Hanns" sort="Ludwig, Hanns" uniqKey="Ludwig H" first="Hanns" last="Ludwig">Hanns Ludwig</name>
<affiliation wicri:level="3"><country xml:lang="fr" wicri:curation="lc">Allemagne</country>
<wicri:regionArea>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Immunological Methods</title>
<title level="j" type="abbrev">JIM</title>
<idno type="ISSN">0022-1759</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1984">1984</date>
<biblScope unit="volume">74</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="337">337</biblScope>
<biblScope unit="page" to="344">344</biblScope>
</imprint>
<idno type="ISSN">0022-1759</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-1759</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antibody</term>
<term>Comparable results</term>
<term>Different fixatives</term>
<term>Fixation</term>
<term>Fixative</term>
<term>Fixative solution</term>
<term>Focus immunoassay</term>
<term>Glutaraldehyde fixation</term>
<term>Growth medium</term>
<term>Horseradish peroxidase</term>
<term>Immune reaction step</term>
<term>Immunoassay</term>
<term>Infectious units</term>
<term>Large scale screening</term>
<term>Microtitre</term>
<term>Microtitre plate</term>
<term>Microtitre plates</term>
<term>Monoclonal antibodies</term>
<term>Plaque</term>
<term>Plaque immunoassay</term>
<term>Secondary antibodies</term>
<term>Substrate solution</term>
<term>Test serum dilutions</term>
<term>Uninfected cells</term>
<term>Viral antigens</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: A new, simple enzyme-linked immunosorbent assay (ELISA) is described which is performed directly on infected and fixed cell cultures in microtitre plates. It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. This immunoassay requires no special equipment and results may be evaluated either with the naked eye or using a light microscope.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001662 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001662 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:FF9244C8CA94116E1DE47356BA3DFA146211FA5A |texte= Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells }}
This area was generated with Dilib version V0.6.33. |